Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 2
2 1 3
3 1 2
4 1 3
5 1 2
6 1 2
7 1 3
8 1 3
9 9 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 74 101
Multiple Base Substitutions 0 0
Deletions 10 39
Insertions 4 10
Mobile Element Insertions 10 14
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 98 164

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 10 4 67.6 2099162 99.4% 2086567 150.4
A1 F50 I2 R1 10 3 82.0 2594444 99.4% 2578877 150.4
A2 F50 I1 R1 13 4 79.4 2453853 99.4% 2439129 150.4
A2 F50 I2 R1 12 3 86.8 2689270 99.2% 2667755 150.4
A2 F50 I3 R1 13 4 61.7 1910099 99.3% 1896728 150.4
A3 F50 I1 R1 47 3 71.7 2268735 99.2% 2250585 150.4
A3 F50 I2 R1 9 3 71.8 2264364 99.3% 2248513 150.4
A4 F50 I1 R1 9 3 69.0 2210911 99.3% 2195434 150.4
A4 F50 I2 R1 9 4 68.8 2134632 99.4% 2121824 150.4
A4 F50 I3 R1 11 3 120.7 3879699 99.0% 3840902 150.4
A5 F50 I1 R1 9 3 70.2 2237825 99.1% 2217684 150.4
A5 F50 I2 R1 9 4 67.2 2152666 99.2% 2135444 150.4
A6 F50 I1 R1 12 4 72.0 2243124 99.1% 2222935 150.4
A6 F50 I2 R1 12 2 61.7 1915694 99.4% 1904199 150.4
A7 F50 I1 R1 8 3 70.3 2237836 99.5% 2226646 150.4
A7 F50 I2 R1 10 3 71.4 2236738 99.2% 2218844 150.4
A7 F50 I3 R1 8 3 65.9 2103080 99.4% 2090461 150.4
A8 F50 I1 R1 11 5 74.5 2292887 99.4% 2279129 150.4
A8 F50 I2 R1 10 5 63.8 1962201 99.4% 1950427 150.4
A8 F50 I3 R1 11 3 75.9 2353494 99.4% 2339373 150.4

Mutation Needle Plot

Mutation Targets

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