Notes

Publication:

10.1371/journal.pone.0151130

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
1 3 4
2 3 4
3 3 4
4 3 4
5 3 4
6 3 4
6 18 24

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 20 46
Multiple Base Substitutions 0 0
Deletions 7 15
Insertions 7 7
Mobile Element Insertions 5 5
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 39 73

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F23 I0 R1 28 0 121.0 2321878 93.0% 2159346 257.6
A1 F23 I1 R1 6 0 120.7 2209470 89.5% 1977475 277.7
A1 F58 I1 R1 6 0 174.1 3313502 94.5% 3131259 259.0
A1 F133 I1 R1 9 0 64.2 1323998 97.6% 1292222 233.4
A2 F23 I0 R1 16 0 118.6 2426804 93.6% 2271488 247.2
A2 F23 I1 R1 6 0 119.8 2393560 93.5% 2237978 246.9
A2 F58 I1 R1 7 0 131.1 2537426 94.2% 2390255 253.5
A2 F134 I1 R1 17 0 226.8 4353178 95.9% 4174697 246.4
A3 F23 I0 R1 27 0 112.4 2123470 92.4% 1962086 262.0
A3 F23 I1 R1 7 0 145.1 2634374 95.1% 2505289 260.6
A3 F57 I1 R1 8 0 141.7 2800920 91.6% 2565642 255.4
A3 F133 I1 R1 8 0 230.9 4597268 94.1% 4326029 246.2
A4 F24 I0 R1 16 0 117.9 2481590 90.1% 2235912 251.0
A4 F24 I1 R1 6 0 97.8 1887382 94.8% 1789238 252.6
A4 F59 I1 R1 8 0 162.1 3105144 95.3% 2959202 252.8
A4 F135 I1 R1 10 0 171.1 3542236 95.1% 3368666 232.2
A5 F24 I0 R1 25 0 119.7 2303815 94.2% 2170193 255.6
A5 F24 I1 R1 9 0 111.5 2050540 94.5% 1937760 264.9
A5 F54 I1 R1 7 0 94.0 1839980 92.2% 1696461 253.6
A5 F135 I1 R1 8 0 249.3 4714410 95.1% 4483403 252.9
A6 F23 I0 R1 23 0 136.8 2742170 94.4% 2588608 254.4
A6 F23 I1 R1 6 0 120.0 2184714 93.1% 2033968 271.0
A6 F58 I1 R1 9 0 94.4 1895438 92.2% 1747593 251.7
A6 F133 I1 R1 14 0 153.6 2843074 95.5% 2715135 257.0

Mutation Needle Plot

Mutation Targets

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