breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 135,162 | C→A | 6.7% | intergenic (+209/‑29) | EO946_RS00715 → / → rpsJ | alpha/beta hydrolase/30S ribosomal protein S10 |
RA | 165,400 | C→T | 6.6% | noncoding (2928/2928 nt) | EO946_RS00950 → | 23S ribosomal RNA |
RA | 240,190 | G→T | 17.1% | F114L (TTC→TTA) | EO946_RS01360 ← | hypothetical protein |
RA | 240,207 | G→T | 11.2% | Q109K (CAA→AAA) | EO946_RS01360 ← | hypothetical protein |
RA | 361,344 | T→G | 6.8% | V144V (GTT→GTG) | EO946_RS01960 → | FAD/NAD(P)‑binding protein |
RA | 361,366 | C→A | 8.1% | Q152K (CAG→AAG) | EO946_RS01960 → | FAD/NAD(P)‑binding protein |
RA | 452,125 | T→A | 42.7% | K146I (AAA→ATA) | EO946_RS02320 ← | dienelactone hydrolase family protein |
RA | 549,085 | C→A | 13.1% | Q33K (CAA→AAA) | EO946_RS02865 → | PLP‑dependent aminotransferase family protein |
RA | 595,180 | G→T | 7.0% | L22F (TTG→TTT) | EO946_RS03130 → | MMPL family transporter |
RA | 607,359 | A→C | 8.9% | T68P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,379 | G→C | 5.7% | T74T (ACG→ACC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,413 | A→C | 9.5% | T86P (ACC→CCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,562 | G→C | 13.4% | G135G (GGG→GGC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,592 | A→C | 21.1% | E145D (GAA→GAC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,594 | C→T | 17.7% | A146V (GCC→GTC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,638 | G→C | 10.0% | A161P (GCC→CCC) | EO946_RS03185 → | collagen‑like protein |
RA | 607,699 | A→C | 5.3% | E181A (GAA→GCA) ‡ | EO946_RS03185 → | collagen‑like protein |
RA | 607,700 | A→C | 12.0% | E181D (GAA→GAC) ‡ | EO946_RS03185 → | collagen‑like protein |
RA | 634,052 | T→G | 17.0% | E573D (GAA→GAC) | EO946_RS03330 ← | ABC‑F family ATP‑binding cassette domain‑containing protein |
RA | 755,530 | A→C | 7.6% | Q22P (CAA→CCA) ‡ | EO946_RS03880 → | rhamnogalacturonan acetylesterase |
RA | 755,531 | A→C | 8.2% | Q22H (CAA→CAC) ‡ | EO946_RS03880 → | rhamnogalacturonan acetylesterase |
RA | 814,356 | T→G | 13.5% | H340Q (CAT→CAG) | nosA → | nitric oxide synthase |
RA | 847,234 | C→A | 6.3% | C284F (TGT→TTT) | EO946_RS04305 ← | NAD(P)‑dependent oxidoreductase |
RA | 986,786 | C→A | 10.0% | Q367K (CAA→AAA) | spoVR → | stage V sporulation protein SpoVR |
RA | 1,097,430 | C→G | 8.7% | S58C (TCT→TGT) | EO946_RS05675 → | MarR family transcriptional regulator |
RA | 1,097,464 | G→T | 7.6% | T69T (ACG→ACT) | EO946_RS05675 → | MarR family transcriptional regulator |
RA | 1,112,969 | C→T | 100% | L1022F (CTT→TTT) | addB → | helicase‑exonuclease AddAB subunit AddB |
RA | 1,293,328 | G→T | 10.7% | R134L (CGA→CTA) | EO946_RS06740 → | sigma‑70 family RNA polymerase sigma factor |
RA | 1,720,712 | C→A | 13.6% | T212T (ACC→ACA) | spoIIIE → | DNA translocase SpoIIIE |
RA | 1,730,080 | A→C | 6.1% | K167N (AAA→AAC) | rodZ → | cell shape determination protein RodZ |
RA | 1,800,874 | A→C | 16.4% | S3519R (AGC→CGC) | EO946_RS09135 → | SDR family NAD(P)‑dependent oxidoreductase |
RA | 1,936,643 | A→C | 5.3% | D3536E (GAT→GAG) ‡ | EO946_RS09735 ← | non‑ribosomal peptide synthetase |
RA | 1,936,644 | T→A | 9.6% | D3536V (GAT→GTT) ‡ | EO946_RS09735 ← | non‑ribosomal peptide synthetase |
RA | 2,073,288 | A→T | 5.5% | G335G (GGT→GGA) | EO946_RS10385 ← | NTTRR‑F1 domain |
RA | 2,073,360 | A→T | 7.3% | G311G (GGT→GGA) | EO946_RS10385 ← | NTTRR‑F1 domain |
RA | 2,103,772 | T→A | 8.7% | V4V (GTA→GTT) | azoJ ← | FMN‑dependent NADH‑azoreductase AzoJ |
RA | 2,139,461 | C→A | 14.7% | A126S (GCG→TCG) | deoD ← | purine‑nucleoside phosphorylase |
RA | 2,147,508 | T→G | 6.3% | T173T (ACA→ACC) | EO946_RS10810 ← | CoA transferase subunit A |
RA | 2,491,058 | T→G | 6.2% | E271D (GAA→GAC) | floA ← | flotillin‑like protein FloA |
RA | 2,627,197 | G→T | 6.1% | intergenic (‑18/‑115) | EO946_RS13595 ← / → EO946_RS13600 | YrzK family protein/SH3 domain‑containing protein |
RA | 2,632,871 | A→G | 5.4% | M575T (ATG→ACG) | recJ ← | single‑stranded‑DNA‑specific exonuclease RecJ |
RA | 2,680,400 | A→T | 10.5% | V417D (GTT→GAT) | EO946_RS13875 ← | LysM peptidoglycan‑binding domain‑containing protein |
RA | 2,812,782 | T→G | 7.1% | K202T (AAG→ACG) | EO946_RS14520 ← | SDR family oxidoreductase |
RA | 2,897,849 | A→C | 6.5% | L106R (CTT→CGT) | EO946_RS14930 ← | hypothetical protein |
RA | 2,978,137 | A→T | 23.3% | noncoding (2664/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
RA | 2,979,628 | T→A | 14.6% | noncoding (1173/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
RA | 2,980,160 | G→A | 16.2% | noncoding (641/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
RA | 2,981,253 | C→T | 37.7% | noncoding (1267/1550 nt) | EO946_RS15435 ← | 16S ribosomal RNA |
RA | 2,982,236 | T→C | 24.2% | noncoding (284/1550 nt) | EO946_RS15435 ← | 16S ribosomal RNA |
RA | 2,991,252 | C→A | 22.5% | intergenic (+53/‑154) | gbsR → / → bslA | transcriptional regulator GbsR/biofilm‑surface layer protein BslA |
RA | 3,111,432 | C→A | 5.1% | intergenic (‑333/+219) | EO946_RS16070 ← / ← EO946_RS16080 | GNAT family N‑acetyltransferase/iron‑sulfur cluster assembly accessory protein |
RA | 3,247,065 | C→T | 100% | intergenic (‑11/+110) | bdbD ← / ← cadA | disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase |
RA | 3,268,725 | C→A | 7.1% | intergenic (+73/+137) | catR → / ← EO946_RS16920 | catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase |
RA | 3,285,567 | C→T | 31.7% | L278L (CTG→CTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,575 | T→G | 32.1% | N276H (AAT→CAT) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,580 | A→G | 35.3% | I274T (ATA→ACA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,647 | C→A | 36.0% | V252L (GTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,285,713 | G→A | 24.5% | L230L (CTA→TTA) | EO946_RS16995 ← | IS3 family transposase |
RA | 3,319,721 | T→G | 5.2% | pseudogene (584/1292 nt) | EO946_RS17170 ← | arabinogalactan endo‑beta‑1,4‑galactanase |
RA | 3,349,666 | T→G | 6.9% | intergenic (+1/+26) | EO946_RS17305 → / ← padC | hypothetical protein/phenolic acid decarboxylase |
RA | 3,375,849 | T→G | 6.7% | N263H (AAC→CAC) | trxB ← | thioredoxin‑disulfide reductase |
RA | 3,446,962 | C→A | 12.2% | M208I (ATG→ATT) | degU ← | two‑component system response regulator DegU |
RA | 3,446,980 | G→T | 14.7% | S202R (AGC→AGA) | degU ← | two‑component system response regulator DegU |
RA | 3,447,038 | T→G | 11.5% | N183T (AAC→ACC) | degU ← | two‑component system response regulator DegU |
RA | 3,498,775 | C→A | 8.0% | Q2K (CAA→AAA) | EO946_RS18005 → | spore germination protein |
RA | 3,517,425 | C→A | 6.5% | H93N (CAT→AAT) | EO946_RS18090 → | SH3 domain‑containing protein |
RA | 3,539,638 | G→T | 8.3% | P105P (CCC→CCA) | EO946_RS18215 ← | T7SS effector LXG polymorphic toxin |
RA | 3,584,291 | G→T | 5.3% | S324S (TCG→TCT) | EO946_RS18420 → | N‑acetylneuraminate synthase family protein |
RA | 3,719,864 | T→G | 7.9% | S79R (AGC→CGC) | EO946_RS19145 ← | YwdI family protein |
RA | 3,721,872 | T→G | 5.0% | E156D (GAA→GAC) | EO946_RS19155 ← | uracil‑DNA glycosylase |
RA | 3,746,893 | T→G | 6.4% | N59H (AAC→CAC) | EO946_RS19275 ← | galactokinase |
RA | 3,746,916 | G→T | 6.4% | T51K (ACA→AAA) | EO946_RS19275 ← | galactokinase |
RA | 3,746,962 | C→A | 8.8% | D36Y (GAT→TAT) | EO946_RS19275 ← | galactokinase |
RA | 3,790,798 | C→A | 5.6% | E191D (GAG→GAT) | EO946_RS19525 ← | BglG family transcription antiterminator |
RA | 3,790,814 | G→T | 9.8% | P186Q (CCA→CAA) | EO946_RS19525 ← | BglG family transcription antiterminator |
RA | 3,846,645 | C→A | 6.1% | D188Y (GAT→TAT) | dbpA ← | ATP‑dependent RNA helicase DbpA |
RA | 3,851,220 | G→T | 9.3% | T52K (ACG→AAG) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,230 | G→T | 6.7% | Q49K (CAG→AAG) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,234 | C→A | 5.1% | M47I (ATG→ATT) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,237 | G→T | 8.1% | I46I (ATC→ATA) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,254 | G→C | 7.8% | L41V (CTT→GTT) | EO946_RS19820 ← | hypothetical protein |
RA | 3,851,262 | C→A | 18.2% | W38L (TGG→TTG) | EO946_RS19820 ← | hypothetical protein |
RA | 3,945,908 | T→G | 9.5% | L388R (CTT→CGT) | EO946_RS20305 → | Na+/H+ antiporter |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP034943 | = 91969 | NA (NA) | 15 (0.050) | 11/250 | NT | NA | intergenic (+131/‑39) | EO946_RS00485/EO946_RS00490 | 16S ribosomal RNA/23S ribosomal RNA |
? | NZ_CP034943 | 91973 = | NA (NA) | intergenic (+135/‑35) | EO946_RS00485/EO946_RS00490 | 16S ribosomal RNA/23S ribosomal RNA | |||||
* | ? | NZ_CP034943 | 607566 = | 207 (0.660) | 87 (0.280) | 37/248 | NT | 22.9% | coding (409/1125 nt) | EO946_RS03185 | collagen‑like protein |
? | NZ_CP034943 | = 607835 | 382 (1.240) | coding (678/1125 nt) | EO946_RS03185 | collagen‑like protein |