Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,106 T→A 6.3% S40T (TCC→ACC)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,1060TA 6.3% 706.1 / 6.1 262S40T (TCC→ACC) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (146/88);  new base A (3/14);  total (151/111)
Fisher's exact test for biased strand distribution p-value = 4.56e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

AGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTG  >  NZ_CP034943/548970‑549244
                                                                                                                                        |                                                                                                                                          
 gTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaa                                                                                                                                        <  1:1875978/140‑1 (MQ=255)
   tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                                                      <  1:2531313/140‑1 (MQ=255)
    aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAg                                                                                                                                     >  2:1250289/1‑140 (MQ=255)
    aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGAAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTGCAAAAg                                                                                                                                     >  1:2822607/1‑140 (MQ=255)
     ggAGAACAACCCAAATGGATATCACGCCTTTATTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGATATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGa                                                                                                                                    <  2:888735/140‑1 (MQ=255)
      gagaACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGAc                                                                                                                                   >  2:285306/1‑140 (MQ=255)
         aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                                                >  1:3485565/1‑140 (MQ=255)
         aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTACCAAAAGACTcc                                                                                                                                >  1:2093801/1‑140 (MQ=255)
          acaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCt                                                                                                                               <  2:172145/140‑1 (MQ=255)
            aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                                             <  1:3903368/140‑1 (MQ=255)
              cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                           >  2:2700869/1‑140 (MQ=255)
                cAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGACTGAAGCTCCCTGCCAAAAGACTCCTGGCGAg                                                                                                                         >  2:3854180/1‑140 (MQ=255)
                  aaTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTc                                                                                                                       <  1:3627051/140‑1 (MQ=255)
                   aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGACAACATGCATCAGGGGCGTCTTCAAATAGGAATGAAGCGCCCTTCCAAACGACTCCTGGCGAGTCa                                                                                                                      >  2:2383398/1‑140 (MQ=255)
                   aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                                      >  1:1087034/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCGCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  1:4174033/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  2:2111358/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  1:4263256/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  1:884836/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  2:142077/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  2:1497930/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  2:2660268/1‑140 (MQ=255)
                        tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                 >  2:859908/1‑140 (MQ=255)
                         atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTCTGCAAAAAGGAATGAAGCTCCCTGCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                >  2:2362822/1‑140 (MQ=255)
                         atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                                                >  2:817109/1‑140 (MQ=255)
                           cccGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGATATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                              <  2:1749806/138‑1 (MQ=255)
                           cccGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTGATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                              <  2:3802327/138‑1 (MQ=255)
                           cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCCGCTTTCCa                                                                                                              >  2:4168710/1‑140 (MQ=255)
                           cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                              >  2:272812/1‑140 (MQ=255)
                           cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                              <  2:4061351/140‑1 (MQ=255)
                             cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                            <  2:1202091/140‑1 (MQ=255)
                              gCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCa                                                                                                           <  1:2353939/140‑1 (MQ=255)
                                cTTTTTTGAATATAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAATGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                         <  2:2574007/140‑1 (MQ=255)
                                cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                         >  1:2694106/1‑140 (MQ=255)
                                cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                         >  1:1008956/1‑140 (MQ=255)
                                cTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                         >  1:4049235/1‑140 (MQ=255)
                                  tttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCa                                                                                                       >  1:1781409/1‑140 (MQ=255)
                                     tttaaTATAAATCTGTATTTTCCGATTTATCAGAAGCTTTATCGCTATTTCAAATAAAACATGCATCTGGGGCTTATTCAAAATGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGATTCAGCTTTCCATCAGTCacac                                                                                                    <  2:2052210/137‑1 (MQ=255)
                                     ttGAATAGAAATCTGTATTTGCCGCTTTACCAGCAGCTTAAGCGCTATTTCAAACAAACCATGAATCAGGGGCTTATTCAAAAAGGAATGAAGCACCCTTCCAACAGACTCCTCGCGAGTCAGCTTTCCATCAGtaacac                                                                                                    <  2:4121651/140‑6 (MQ=255)
                                          tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTg                                                                                               >  1:3538012/1‑140 (MQ=255)
                                                 cgggATATTCCGGTTTATCACCAGCTTTATCGCTATTTCAAACAAAACATGCACCCGGGGCGTATTAAAAACGCAATGAAGCTCCCTTCCAACCGACTCCTGGCGAGTCAGGTTTCCATCTGTCACACGACTGTAGAACg                                                                                        <  1:3583968/138‑1 (MQ=255)
                                                 cTGTATATTCCGCTGTACCCGCAGCTTTATCGCTATTTCAAAGAAAACATGAAGCCGGGGCGTATTAAAAAAGCAATCACGCGCCCGTCCAAACGACGCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACg                                                                                        <  1:192143/140‑1 (MQ=255)
                                                 cTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAACAAAACATGCATCCGGGGCGTATTCAAAACGGAATGACGCACCCTTCCAAACCACGCCGGGCGAGTAAGGTTTCCATCAGTCAAACGACTGTAGAACg                                                                                        <  1:4014748/140‑1 (MQ=255)
                                                 cTGGATATTCCGCTTTAACAGCAGCTTTATCGCTATTTAAAACAAAACATGCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGCCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACg                                                                                        <  2:3096703/140‑1 (MQ=255)
                                                   ggATTTTCCGATTAATCAGCAGCTTTATCGGTATTACAAATAAAACATGATTCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTACCAAAAGACTCCTGGGGAGTCAGATTACCATCAGTCAAACGACTGTAGAACGTg                                                                                      <  2:2554833/140‑1 (MQ=255)
                                                    gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                     >  2:163564/1‑140 (MQ=255)
                                                         tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGt                                                                                                                        >  2:58370/1‑100 (MQ=255)
                                                         tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                                                >  2:178222/1‑140 (MQ=255)
                                                         tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                                                >  2:1889357/1‑140 (MQ=255)
                                                           ctcTTTATCAGCAGATTTATCGATATTTCAAAAAAAACATGCAACCGGGGCTTATTAAAAACGCAATCAAGCTCCCTTCCAAAAGACTCCTGGCGATACAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGaa                                                                              <  1:2666469/138‑1 (MQ=255)
                                                           cGCTTTATCCGCAGCTTTATCGATATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAACGGAATCAAGCTCCCTTCAAAAAGACCCCTGGCGCTTCAGCTTTCCATCAGCCAAACGACTGTAGAACGTGCCTATGaa                                                                              <  1:57369/140‑1 (MQ=255)
                                                           cGCTTTATCAGCAGTTTTTTCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCGCCCTTCCAAACGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGaa                                                                              <  2:533502/140‑1 (MQ=255)
                                                           cGCTTTATCAGCAGCTTTATCGATATTAAAAATAAAAAATGCATCAGGGGCTTATTAAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCGGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGaa                                                                              <  2:1437429/140‑1 (MQ=255)
                                                           cGCTTTACCAGCAGCTTTATCGCTATTACAAACAAAACATGAATCCGGGGCTTATAAAAAAAGCAATGACGCGCCCTACCAAACCACGCCGGGCGAGTCAGCTTTCCACCCGCCACACGACTGTAGAACGTGCCTATGaa                                                                              <  1:1250289/140‑1 (MQ=255)
                                                              tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                                           >  1:2049561/1‑140 (MQ=255)
                                                                tATCACCACCTTTATCCATATTTAAAAGAAAACATCCAACAGGGGGTTATACAAAATGCAATTAAGCTACCTTCAAAAAGACTCCTGGCGTTTCAGGTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCt                                                                         <  2:2746958/140‑1 (MQ=255)
                                                                  tCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCCGGGGCGTATACAAAACGCAATGACGCGCCCTTCCAAAACACCCCCGGCGAGTCAGCTTTCCAACAGACAAACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                       <  1:666670/140‑1 (MQ=255)
                                                                  acagcagATTTATCGCTATTTCAAACAAAACATGCATCCGGGGCGTATTCAAAAAGTAATTAAGCTCCCTTCAAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                       <  1:1982887/139‑1 (MQ=255)
                                                                       cgcTTTACCGATTTTCCAACAAAAACAGCCATCCGGGGCGTTTTAAAAACGCATTGACGCCCCCTACCAAACCCCCCCGGGCGAGTCAGCTTCCCCTCACACACACGACTGTAGAACGTGCCTAGGAACAGCTTgctgct                                                                  <  1:3419210/139‑1 (MQ=255)
                                                                       agcTTTATCGCTTTTTCAAACAAAACCTGCATCCGGGGCGTATAAAAAACGCAATGAAGCGCCCTCCCAAACGCCGCCGGGCGAGTCACCTTGCCACCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                  <  1:3265407/140‑1 (MQ=255)
                                                                       agcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                  >  1:2271405/1‑140 (MQ=255)
                                                                       agcTTTATCGATATTTCAAATAAAACCTGCAACCGGGGCGTTTTAAAAAAGCAATGACGCCCCCTTCCAAAACACGCCGGGCGAGTCAGCTTTCCACCACCCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                  <  1:1445163/140‑1 (MQ=255)
                                                                       agcTTGATCGCTATTAAAAACAAAACACCCAGCCGGGGGGTTTACAACACCGAATCAACCGCCAGTCCAACACACCCCGGCCGAGTAAGCTTCCCACCAGCCCCACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                  <  1:2225629/140‑1 (MQ=255)
                                                                        gcTTTATCGCTATTTCAAAGAAAACAGGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAACGACGCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                 <  2:2509003/140‑1 (MQ=255)
                                                                        gatttATCGATATTTCAAATAAAACATGAATAAGGGGCGTATTAAAAAAGTAATTAAGCTCCTTTCCAAAAGACACCGGGCGAGTAAGGTTTCCAGCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                 <  2:3326803/138‑1 (MQ=255)
                                                                         cTTTCTCGCTATTTCAAAGAAACCATCCATCCGGGGCGTATTAAAAAAGGAAGGAAGCGCCCTTCCAAAACACCCCTGGCGAGTCTGCTTTCCACCAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                <  2:2917775/140‑1 (MQ=255)
                                                                         cTTTATCGCTTTTACAAAGAAAAAATCAAACAGGGGCTTATTAAAAAAGTAATGAAGCTCCATACCAAAAGACACCTGGCGAGTCAGCTTTCCATCCGCCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                <  2:3165661/140‑1 (MQ=255)
                                                                         cTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAACGACTCCGGGCGAGTAAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                <  2:1343835/140‑1 (MQ=255)
                                                                         cTTTAACCCTATTACAAACAAAACATCAATCCGGGGCTAATTCAAAAAGTAATTAAGCCCCCTTCCAAAACACTCCTGGCGAGTCACCTTACCATAATTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                <  2:1570414/140‑1 (MQ=255)
                                                                            tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                                             >  1:3029413/1‑140 (MQ=255)
                                                                            tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                                             >  1:3842471/1‑140 (MQ=255)
                                                                            tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                                             >  1:1510951/1‑140 (MQ=255)
                                                                                   aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                      >  2:1033501/1‑140 (MQ=255)
                                                                                         aacaaaaCATGCATCAGGGGCGTATTCAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                <  1:1896431/137‑1 (MQ=255)
                                                                                              aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGcc                                           >  1:1327939/1‑140 (MQ=255)
                                                                                                 aTGCATCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCAAAAAGACTCCTGGCGAGTCAGCTGTCCAACCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaag                                        <  1:2111358/140‑1 (MQ=255)
                                                                                                      tCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTCTGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCg                                   >  2:1994823/1‑140 (MQ=255)
                                                                                                       ccggggCGTATACAAAACGTAATGAAGCGCCCTTCCAACCCCCTCCTGGCGAGTCTGCTTTCCAACAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCgg                                  <  1:509378/138‑1 (MQ=255)
                                                                                                        aGGGGCGTCTTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGa                                 >  2:1712136/1‑140 (MQ=255)
                                                                                                        aGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGa                                 >  2:281010/1‑140 (MQ=255)
                                                                                                         ggggCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGAt                                >  2:18781/1‑140 (MQ=255)
                                                                                                            gcttATTAAAAACGGAATGAAGCTCCCTCCAAAACCACGCCGGGCGAGTAAGCTTTCAACCCTTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGt                             <  1:1404748/137‑1 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGtgtg                          >  1:2518570/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  1:1624976/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  2:984609/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  2:3912042/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  1:2970108/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  1:3736133/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  1:998199/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  2:2179701/1‑140 (MQ=255)
                                                                                                               tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAAAGTGCCTATGAACAGCTTGCTGCGGAGGGCTATCTCGTGAGCAAGCCAAGAAGCGGATGGTTTg                          >  2:2973667/1‑140 (MQ=255)
                                                                                                                 ttaaaaatGGAATGACGCTCCCTTCCAAAAGACTCCTGGCGAGTAAGGTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt                        <  2:2271405/137‑1 (MQ=255)
                                                                                                                 ttCAAAAAGGAATGACGCGCCCTTCCAAACGACTCCTGGCGAGTCTGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt                        <  2:2453084/140‑1 (MQ=255)
                                                                                                                 ttCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt                        >  2:2735112/1‑140 (MQ=255)
                                                                                                                    aaaaaGTAATTACGCTCCCTTACTACTGTCTCCTTGCTAGTCATCTTTCCATAAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAt                     <  2:3596413/140‑1 (MQ=255)
                                                                                                                    aaaaaGTAATGAAGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCATCAGTAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAt                     <  2:1087034/140‑1 (MQ=255)
                                                                                                                        aGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATc                 >  1:1750366/1‑140 (MQ=255)
                                                                                                                                  cTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGa       >  2:1646283/1‑140 (MQ=255)
                                                                                                                                    cccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCACGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGagt     >  2:3412093/1‑138 (MQ=255)
                                                                                                                                      attCAAAAAGAATCCTGGGGTGTCAGCTTTCCATAAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAtttt   <  1:4112664/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTG  >  NZ_CP034943/548970‑549244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: