Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 845,030 G→A 5.3% G311G (GGC→GGT yfkAB ← radical SAM/CxCxxxxC motif protein YfkAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943845,0300GA 5.3% 649.4 / 16.6 189G311G (GGC→GGTyfkABradical SAM/CxCxxxxC motif protein YfkAB
Reads supporting (aligned to +/- strand):  ref base G (89/90);  new base A (5/5);  total (94/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 195 total aligned reads displayed.

TTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGC  >  NZ_CP034943/844893‑845169
                                                                                                                                         |                                                                                                                                           
ttGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGcc                                                                                                                                           <  1:858443/140‑1 (MQ=255)
         aCATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                  <  1:971391/140‑1 (MQ=255)
                  ccGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAAt                                                                                                                         >  2:3065412/1‑140 (MQ=255)
                    gAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGt                                                                                                                       >  2:69470/1‑140 (MQ=255)
                    gAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGt                                                                                                                       >  2:2803650/1‑140 (MQ=255)
                     aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                      >  2:95702/1‑140 (MQ=255)
                     aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                      >  2:1786982/1‑140 (MQ=255)
                     aGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGtt                                                                                                                      >  2:212924/1‑140 (MQ=255)
                          tCGGACATTCGGACAGTGGGAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGt                                                                                                                 <  2:2310389/140‑1 (MQ=255)
                           cGGACATTCGGACCGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCGGTCTGAAGATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATCATGTTTCCGTc                                                                                                                >  2:3518218/1‑140 (MQ=255)
                           cGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTc                                                                                                                >  1:3277774/1‑140 (MQ=255)
                           cGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCGTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTc                                                                                                                >  2:2824491/1‑140 (MQ=255)
                                aTTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCaaaaa                                                                                                           <  2:1978924/140‑1 (MQ=255)
                                  tCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                                         >  1:2396924/1‑140 (MQ=255)
                                  tCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATg                                                                                                         >  1:2703960/1‑140 (MQ=255)
                                    ggACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGtt                                                                                                       <  1:3870816/140‑1 (MQ=255)
                                    ggACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGtt                                                                                                       <  1:1237320/140‑1 (MQ=255)
                                     gACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTc                                                                                                      <  1:2125014/140‑1 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGt                                                                                                                                  >  1:2840739/1‑107 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  1:1697560/1‑140 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  2:1908799/1‑140 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  2:2744913/1‑140 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  2:534434/1‑140 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  1:2329611/1‑140 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTTGGTCTGTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGACTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  1:1478035/1‑140 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCTGGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  1:3461761/1‑140 (MQ=255)
                                          tGGCAATTCAGTTCTTTCGCAAGCGTGGTCTGTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCCGCGGAATATTGCCGAGCGGCGGTGTATCGCCCAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACAtt                                                                                                 >  1:2613094/1‑140 (MQ=255)
                                            gCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCa                                                                                               >  1:1170007/1‑140 (MQ=255)
                                               aTTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGc                                                                                            <  1:3283441/140‑1 (MQ=255)
                                                ctCAGTTCTTTCGCAAGCTTGGTCTCTCTCCACTTTTCATAGGCGGAAGGGAGGCTGTCCGTCTGAATGTTGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCg                                                                                           >  1:1987208/2‑140 (MQ=255)
                                                    gTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGaa                                                                                       <  1:416692/140‑1 (MQ=255)
                                                     ttCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGTCGGGAAc                                                                                      >  1:2928108/1‑140 (MQ=255)
                                                     ttCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAAc                                                                                      >  1:172009/1‑140 (MQ=255)
                                                      tCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACg                                                                                     <  1:3759504/140‑1 (MQ=255)
                                                       cTTTCGCAAGCTTGGTCTGTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACgg                                                                                    <  2:1793109/140‑1 (MQ=255)
                                                        tttCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGc                                                                                   >  1:3142611/1‑140 (MQ=255)
                                                               aGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCAGATTCAGGAGGGAACGTCCGTCAgg                                                                            >  2:3360008/1‑140 (MQ=255)
                                                                gCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAACATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGa                                                                           <  2:3273472/140‑1 (MQ=255)
                                                                 cTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGAt                                                                          <  1:3264060/140‑1 (MQ=255)
                                                                 cTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGAt                                                                          <  1:335711/140‑1 (MQ=255)
                                                                   tGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCa                                                                        <  1:887595/140‑1 (MQ=255)
                                                                   tGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCa                                                                        <  1:1499468/140‑1 (MQ=255)
                                                                   tGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCa                                                                        <  1:3743152/140‑1 (MQ=255)
                                                                    ggTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCAt                                                                       >  2:3909026/1‑140 (MQ=255)
                                                                        tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                   <  1:1101010/140‑1 (MQ=255)
                                                                        tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                   <  1:4136491/140‑1 (MQ=255)
                                                                        tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                   <  1:3853355/140‑1 (MQ=255)
                                                                        tttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                   <  1:3496807/140‑1 (MQ=255)
                                                                        gttCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTtct                                                                   <  1:3496837/139‑1 (MQ=255)
                                                                          tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                                 <  1:2972878/140‑1 (MQ=255)
                                                                          tctcCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCa                                                                 >  2:2864731/1‑140 (MQ=255)
                                                                               aTTTGTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTCTCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTg                                                            <  1:3980812/140‑1 (MQ=255)
                                                                                tttttCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGa                                                           >  2:2594787/1‑140 (MQ=255)
                                                                                          gCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGc                                                 <  1:3601387/140‑1 (MQ=255)
                                                                                           cGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCt                                                >  2:4202525/1‑140 (MQ=255)
                                                                                             gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCCAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTcc                                              >  2:2951421/1‑140 (MQ=255)
                                                                                             gAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGGCAAAAATGTTCACATTCCGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTGTTTCGCTcc                                              >  2:2656615/1‑140 (MQ=255)
                                                                                                     gCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGCCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGcc                                      >  1:2052577/1‑140 (MQ=255)
                                                                                                     gCTGTCCGGCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGGCTCGATAATGTTTCCGTCAAAAATGTTCACATGCTGGCGGGACCGGCCGTCAGGATCCTTTCTCACGGTGACGTTTTTCGCTGCGTGACGcc                                      >  2:3214615/1‑140 (MQ=255)
                                                                                                      cTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCg                                     >  1:1598033/1‑140 (MQ=255)
                                                                                                        gTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGc                                   >  1:2065360/1‑140 (MQ=255)
                                                                                                        gTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGc                                   >  1:3196481/1‑140 (MQ=255)
                                                                                                         tCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCg                                  >  2:4166967/1‑140 (MQ=255)
                                                                                                         tCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCg                                  >  2:2824403/1‑140 (MQ=255)
                                                                                                            gTCTGAATATTGCCTAGCGGCGTTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                               <  2:3827727/140‑1 (MQ=255)
                                                                                                            gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                               <  1:534434/140‑1 (MQ=255)
                                                                                                            gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                               <  1:471830/140‑1 (MQ=255)
                                                                                                            gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                               <  2:169770/140‑1 (MQ=255)
                                                                                                            gTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAag                               >  1:3937773/1‑140 (MQ=255)
                                                                                                              cTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAagca                             <  1:1366051/140‑1 (MQ=255)
                                                                                                              cTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAagca                             <  1:2479052/140‑1 (MQ=255)
                                                                                                                   tatTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCTGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCttt                        >  1:1898257/1‑140 (MQ=255)
                                                                                                                   tatTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCttt                        >  1:3079558/1‑140 (MQ=255)
                                                                                                                      tGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGa                     >  2:571986/1‑140 (MQ=255)
                                                                                                                      tGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGa                     >  2:1512952/1‑140 (MQ=255)
                                                                                                                      tGCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg                     <  2:2467521/140‑2 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTTTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  1:4212481/140‑1 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  1:2429454/140‑1 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  1:2349715/140‑1 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  2:1076878/140‑1 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  1:904153/140‑1 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  1:1342003/140‑1 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  1:4248954/140‑1 (MQ=255)
                                                                                                                       gCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGAt                    <  1:1262861/140‑1 (MQ=255)
                                                                                                                       gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg                     <  2:3817382/139‑2 (MQ=255)
                                                                                                                       gCCAAGCGGCGGGGTATCACCGAAATCGGTCACGATAATGTTTCCGTCGAAAATGTTCACATTCAGGCGGGATCGGCCGTCAGGGTCATTTCGCACTGTGACGTTTTTCGCCGCGCGAAGCCGGCGAAGCAGCTTTTgg                     >  1:3817382/1‑138 (MQ=255)
                                                                                                                        ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc                   >  1:2749599/1‑140 (MQ=255)
                                                                                                                        ccTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATc                   >  1:627498/1‑140 (MQ=255)
                                                                                                                          tAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGt                 <  1:1674132/140‑1 (MQ=255)
                                                                                                                          tAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGt                 <  1:363184/140‑1 (MQ=255)
                                                                                                                          tAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGt                 <  1:1588680/140‑1 (MQ=255)
                                                                                                                           aGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTc                >  1:1546967/1‑140 (MQ=255)
                                                                                                                                 ggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTTACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTc          <  2:3277774/140‑1 (MQ=255)
                                                                                                                                 ggTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTc          <  1:4171109/140‑1 (MQ=255)
                                                                                                                                 ggTGTAGCGCCAAAATCGTTCACGATAATGTGTCCGTCAAAAATGTTCACATTCAGGGGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTc          <  1:2310577/140‑1 (MQ=255)
                                                                                                                                  gtgtATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTcc         >  2:3600869/1‑140 (MQ=255)
                                                                                                                                       tCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACt    >  1:1867484/1‑140 (MQ=255)
                                                                                                                                         gCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCCCCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGc  <  2:1266288/140‑1 (MQ=255)
                                                                                                                                         gCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGc  <  2:1478035/140‑1 (MQ=255)
                                                                                                                                         gCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGc  <  2:2703960/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TTGACGAGAACATTCGGTCCGAGGCATCGGACATTCGGACAGTGGCAATTCAGTTCTTTCGCAAGCTTGGTCTTTCTCCATTTTTCATAAGCGGAAGGGAGGCTGTCCGTCTGAATATTGCCTAGCGGCGGTGTATCGCCAAAATCGGTCACGATAATGTTTCCGTCAAAAATGTTCACATTCAGGCGGGAACGGCCGTCAGGATCATTTCTCACCGTGACGTTTTTCGCTCCGTGAAGCCGGCGAAGCAGCTTTTGATCGTCTTCGTCCTGACTGC  >  NZ_CP034943/844893‑845169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: