Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,014,009 G→T 8.1% R56R (CGC→CGA EO946_RS05230 ← polysaccharide deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,014,0090GT 8.1% 595.7 / 12.0 245R56R (CGC→CGAEO946_RS05230polysaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base G (99/118);  new base T (26/2);  total (125/120)
Fisher's exact test for biased strand distribution p-value = 9.71e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAA  >  NZ_CP034943/1013871‑1014147
                                                                                                                                          |                                                                                                                                          
        ttGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTgg                                                                                                                                   >  1:1176914/1‑140 (MQ=255)
        ttGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTgg                                                                                                                                   >  2:1215911/1‑140 (MQ=255)
         tGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGc                                                                                                                                  <  2:4144393/140‑1 (MQ=255)
         tGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCATCAGGCTTTCCTTGTCATTGAGAAGCTGGc                                                                                                                                  >  1:2396753/1‑140 (MQ=255)
               ggCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTGAGCTTCAGGCTTTCCTTCTCATTGCGAACCTGGCCGtttt                                                                                                                            >  1:3663929/1‑139 (MQ=255)
               ggCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTtg                                                                                                                            <  2:3175116/140‑1 (MQ=255)
               ggCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTtg                                                                                                                            <  2:2997864/140‑1 (MQ=255)
                gCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGttttt                                                                                                                           >  1:452998/1‑138 (MQ=255)
                gCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTtgt                                                                                                                           >  2:1969170/1‑140 (MQ=255)
                gCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTtgt                                                                                                                           >  2:2290485/1‑140 (MQ=255)
                gCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTtgt                                                                                                                           >  1:225274/1‑140 (MQ=255)
                gCTGACGGGCCATCATCGAAGGATAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCACATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTACTTCTCATTGCGAAGCTGTCCGTTtgt                                                                                                                           >  2:3090350/1‑140 (MQ=255)
                 cTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCATGCTTTCCTTCTCATTGCGAAGCTGGCCGTTtttg                                                                                                                          >  1:3278044/1‑140 (MQ=255)
                 cTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCGTAAGGCTTTCCTTCTCATTTCGAAGCTGGCCGTTtgtg                                                                                                                          >  1:522837/1‑140 (MQ=255)
                   gACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATAAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTATCATTGCGAAGCTGGCCGTTtgttgt                                                                                                                        >  2:565655/1‑140 (MQ=255)
                    aCGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGGTTttggtg                                                                                                                       >  2:698018/1‑140 (MQ=255)
                     cgtcacatcatCGAAGGTTAATTAAAAAGTCAAATCTAAAATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      >  2:1895355/6‑140 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTGAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      >  1:1975186/1‑140 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      <  1:3710894/140‑1 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      >  2:1575610/1‑140 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      >  2:2220976/1‑140 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      <  1:3758465/140‑1 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      <  1:2375394/140‑1 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      <  1:4089700/140‑1 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCAATGCGAAGCTGGCCGTTTGtggtgg                                                                                                                      >  1:940725/1‑140 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTCCTTCTCATTTCGAAGCTGGCCGGTTGttgtgg                                                                                                                      >  1:2424366/1‑140 (MQ=255)
                     cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTAATTCTTTCCTTATAATTTCGAACCTTGCCGTTTTttgtgg                                                                                                                      >  1:2348320/1‑140 (MQ=255)
                     cGGGCCATCATAGAAGGTTAAGTAAAACGACTTATCTCCCATGTTCTGCTTTTTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCCGGCTTTCCTTCTCAGTGCGAAGCTGGCCGGTTGtggtgg                                                                                                                      <  1:3681780/140‑1 (MQ=255)
                          catcatCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTTGGGTGGCAGTg                                                                                                                 >  1:1309659/1‑140 (MQ=255)
                          catcatCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCGGCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTGGTGGTGGCAGTg                                                                                                                 >  2:1414985/1‑140 (MQ=255)
                           atcatcGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGGTTGGGGTGGGCGTGa                                                                                                                >  1:2982604/1‑140 (MQ=255)
                                    ggTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTTTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTGAGCTTGAGGCTTTGCTTGTAATTGCGTAGCTGGCCGTTTGTGGTTGCAGTGACTTCCtttt                                                                                                       >  2:1497497/1‑139 (MQ=255)
                                        aaGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCttttgttt                                                                                                   >  1:1476814/1‑135 (MQ=255)
                                         aGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTc                                                                                                  >  2:1816187/1‑140 (MQ=255)
                                         aGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTc                                                                                                  >  2:2075478/1‑140 (MQ=255)
                                             aaCCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTTCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATg                                                                                              >  1:3598114/1‑140 (MQ=255)
                                             aaCCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATg                                                                                              <  2:173090/140‑1 (MQ=255)
                                              aCCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGa                                                                                             <  1:4160420/140‑1 (MQ=255)
                                               ccGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAg                                                                                            >  2:729309/1‑140 (MQ=255)
                                                cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                           <  2:2891959/140‑1 (MQ=255)
                                                cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                           <  1:605146/140‑1 (MQ=255)
                                                cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                           <  2:2950198/140‑1 (MQ=255)
                                                cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTGGACGCGTTGCAGGTCATTGACAAGCTTGCCCGTTTTGGTGGCAGTTAATTACTTTTGTTTAGAttatc                                                                                           >  1:1392333/1‑136 (MQ=255)
                                                cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCACCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                           <  1:549134/140‑1 (MQ=255)
                                                cGTCTTATCTCCCAGGTTCTGCTGTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                           <  2:2345287/140‑1 (MQ=255)
                                                  tCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCGTTACTTCTCATTGCGAAGCTGGCCGTTTTTTGTGGCAGTGACTTACTTTTCTTTAGATGAGGTg                                                                                         >  1:2653327/1‑140 (MQ=255)
                                                   cTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCGTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGt                                                                                        >  2:800387/1‑140 (MQ=255)
                                                       tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                                                                    <  1:1685397/140‑1 (MQ=255)
                                                       tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                                                                    <  2:1176914/140‑1 (MQ=255)
                                                       tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                                                                    <  1:729309/140‑1 (MQ=255)
                                                       tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                                                                    <  1:308044/140‑1 (MQ=255)
                                                        ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                   <  1:1808823/140‑1 (MQ=255)
                                                        ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                   <  1:3913187/140‑1 (MQ=255)
                                                        ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                   <  1:3821739/140‑1 (MQ=255)
                                                        ctcCCATGTTCTGCTTTGTCTGCAGATGTTGTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                   <  2:274834/140‑1 (MQ=255)
                                                            cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTTCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTACATGAGCTGTTGGAATAcc                                                                               >  1:4243662/1‑140 (MQ=255)
                                                            cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTTACTTACTTTGCTTTCGAAGAGCTGTTGGtataac                                                                               >  2:3821739/1‑138 (MQ=255)
                                                            cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCGTAAGGGGTTGCTTCTCATTTCGAAGCTGGGCGGTTTTGGTGGCACTTTCTTACATTTCTTTAGATGAGGTTTTTGAATaac                                                                               >  1:2135558/1‑138 (MQ=255)
                                                               gTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTa                                                                            <  2:3518285/140‑1 (MQ=255)
                                                                ttCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTAAGGCTTTCCTTATCATTGCGAAGCTGGCCGTTTGTGGTGGCAGGTACTTACATTTGTTTCGATGAGCGGTTTGAATAcacaat                                                                           >  1:1640189/1‑135 (MQ=255)
                                                                   tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                                                                        <  1:3020412/140‑1 (MQ=255)
                                                                   tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                                                                        <  2:1292488/140‑1 (MQ=255)
                                                                     cTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGGTTCAGGCTTTGCGTCTCATTGGGAAGCGGGCCGTTTGTGGGGGCAGTGACTTGCTTTTCTTTGGATGTGCTGTTTGTATAACCGAgcagaa                                                                      >  1:2719569/1‑135 (MQ=255)
                                                                     cTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                   <  1:2285194/127‑1 (MQ=255)
                                                                          tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTGCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTTCGTTAGATGAGCTGTTTGAATAACCAAGAAAAACCaaa                                                                 >  1:2096104/1‑138 (MQ=255)
                                                                          tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAATCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCaaa                                                                 >  2:3066970/1‑138 (MQ=255)
                                                                          tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTGCTTTTCTTTCGTTGAGCTGTTGGAATACCCTAGAATCACCATa                                                                 >  2:1164190/1‑140 (MQ=255)
                                                                          tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCGTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGACTACCCTAGAATCACCATa                                                                 >  2:2460699/1‑140 (MQ=255)
                                                                          tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGGTTTCCTTCTCATTTCGAAGCTGGCCGTTTTTGGTGGCACTGACTTCCTTTTCTTTAGATGAGCTGTTGGGATAACCGATAATAACCaaa                                                                 >  1:1850775/1‑138 (MQ=255)
                                                                          tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTACTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTTGTTTCGATGTGCTGTTTGGATAACCTAGAATCACCaaa                                                                 >  1:2746843/1‑138 (MQ=255)
                                                                          tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTCTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATa                                                                 >  2:2783998/1‑140 (MQ=255)
                                                                             gCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGc                                                              <  1:1352333/140‑1 (MQ=255)
                                                                             gCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGc                                                              <  1:104226/140‑1 (MQ=255)
                                                                               aGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTGAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTAGATGATCTGTTTGTATAACCTAGAATCACCATAAGCaa                                                            >  2:4184279/1‑140 (MQ=255)
                                                                               aGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTGTGTGGTGGCACTGACTTACTTTGGGTTGGATGAGCGGTTGGGATACCCAATACTCACCAAAAGCaa                                                            >  1:3196645/1‑140 (MQ=255)
                                                                               aGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTACTCTTCATGCGTTCCTTATCATTGCGGAGCAGGGCGTTTGTGGTTGCAGTGACTTCCATTGCTTTAGATTAGCAGTTTGAAAACACTAGAATCAACATAAGCaa                                                            >  2:889021/1‑140 (MQ=255)
                                                                                  tGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAag                                                         <  1:786253/140‑1 (MQ=255)
                                                                                    ttttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTGAGGCTTTCCTTGTCATTTGGAAGCTGGCCGTTTGTTGTGGCAGTGACTTCCATTGGTTTCGATGAGCGGTTTGGATACCCGATAATGACCATAACCAAAaaca                                                       >  1:1538043/1‑140 (MQ=255)
                                                                                    ttttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGGCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTAGATGAGCTGTTTGAATTCCCTAGAATCACCATAAGCAAAagca                                                       >  2:593542/1‑140 (MQ=255)
                                                                                    ttttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGAt                                                                                               <  1:2859204/100‑1 (MQ=255)
                                                                                    ttttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagca                                                       >  2:4015394/1‑140 (MQ=255)
                                                                                    ttttATCAGCAGATCGGGGGAGTCGTTTTTCAGCGTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGGTTTCGATGAGCTGTTGGAAAACCCTACAATCACCATAAGCAAAagca                                                       >  2:3243962/1‑140 (MQ=255)
                                                                                      ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                     <  2:225274/140‑1 (MQ=255)
                                                                                      ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                     <  1:2919479/140‑1 (MQ=255)
                                                                                      ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                     <  1:1828136/140‑1 (MQ=255)
                                                                                      ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                     <  2:3966423/140‑1 (MQ=255)
                                                                                      tgaTCCGCAGATCGGGGGCCTCGTTTTTCAGCTTCAGGCTTGCCTGCTCCTTGCGAAGCTGGCCGTTTGTGGTGGCCGTGTCTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                     <  1:3418162/138‑1 (MQ=255)
                                                                                      ggaTCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCGGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                     <  1:3553402/138‑1 (MQ=255)
                                                                                              agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                             <  2:2405492/140‑1 (MQ=255)
                                                                                              agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                             <  2:340748/140‑1 (MQ=255)
                                                                                              agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                             <  2:1197248/140‑1 (MQ=255)
                                                                                              agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCAGTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTGTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                             <  1:417468/140‑1 (MQ=255)
                                                                                                          tCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                                                                        >  2:1333544/1‑101 (MQ=255)
                                                                                                                            cTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAaagcaag               <  1:2066295/140‑1 (MQ=255)
                                                                                                                            cTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACGTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAaagcaag               <  1:3509012/140‑1 (MQ=255)
                                                                                                                               tCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGa            >  1:3054186/1‑140 (MQ=255)
                                                                                                                                ccTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGaa           <  1:2572703/140‑1 (MQ=255)
                                                                                                                                  ttCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAAt         <  1:999390/140‑1 (MQ=255)
                                                                                                                                         tGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGaa  >  1:2218663/1‑140 (MQ=255)
                                                                                                                                         tGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAAATGCTAAACTTGAAAATTTAAAATCAAGCGAAATCGCAGaa  >  1:3337843/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAA  >  NZ_CP034943/1013871‑1014147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: