Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,176,173 G→T 10.8% G135V (GGT→GTT)  med → transcriptional regulator Med

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,176,1730GT10.8% 710.4 / 56.5 255G135V (GGT→GTT) medtranscriptional regulator Med
Reads supporting (aligned to +/- strand):  ref base G (121/105);  new base T (19/10);  total (140/115)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

CTCTATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAACACA  >  NZ_CP034943/1176034‑1176312
                                                                                                                                           |                                                                                                                                           
ctctATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATggg                                                                                                                                             >  1:1441396/1‑140 (MQ=255)
ctctATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATggg                                                                                                                                             >  1:2590964/1‑140 (MQ=255)
ctctATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATggg                                                                                                                                             >  1:3371265/1‑140 (MQ=255)
ctctATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATggg                                                                                                                                             >  1:699496/1‑140 (MQ=255)
     tGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGttttt                                                                                                                                        >  2:3302648/1‑140 (MQ=255)
     tGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTAAGCGGCGAAGCAATGGGttttt                                                                                                                                        >  2:2817977/1‑140 (MQ=255)
          aTGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTgga                                                                                                                                   >  2:2506704/1‑140 (MQ=255)
          aTGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTgga                                                                                                                                   >  2:2100957/1‑140 (MQ=255)
          aTGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTgga                                                                                                                                   >  2:1962139/1‑140 (MQ=255)
             ggAGTGAATATGCAGAGGTGTATAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATATATAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTACCGCTGCGAAGCAATGGGTTTTTTTggagga                                                                                                                                >  2:2440968/1‑140 (MQ=255)
                 tGAATGTGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGa                                                                                                                            >  1:2255455/1‑140 (MQ=255)
                 tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGt                                                                                                                                            >  1:1836832/1‑124 (MQ=255)
                 tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGt                                                                                                                                            <  2:1836832/124‑1 (MQ=255)
                         cAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGTGGAATGACAGCTGcc                                                                                                                    >  2:1122840/1‑140 (MQ=255)
                         cAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGcc                                                                                                                    >  1:864908/1‑140 (MQ=255)
                           gagGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCa                                                                                                                  >  2:4206676/1‑140 (MQ=255)
                           gagGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCa                                                                                                                  >  1:1001004/1‑140 (MQ=255)
                            agGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCAt                                                                                                                 <  1:1536426/140‑1 (MQ=255)
                            agGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCAt                                                                                                                 <  2:701479/140‑1 (MQ=255)
                            agGTCTTGAACTTGGTCAGCGAAGATTACCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCAt                                                                                                                 <  1:3214333/140‑1 (MQ=255)
                                   tAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGa                                                                                                          >  1:3605359/1‑140 (MQ=255)
                                      cTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGaaaa                                                                                                       >  1:229635/1‑140 (MQ=255)
                                      cTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGaaaa                                                                                                       <  1:4082049/140‑1 (MQ=255)
                                      cTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGaaaa                                                                                                       <  1:2765317/140‑1 (MQ=255)
                                        tGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACg                                                                                                     >  1:139505/1‑140 (MQ=255)
                                           tCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAAt                                                                                                  >  2:3121225/1‑140 (MQ=255)
                                               cGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGt                                                                                                                                            >  1:819991/1‑94 (MQ=255)
                                                gAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                             <  2:3098344/140‑1 (MQ=255)
                                                gAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                             <  2:2943093/140‑1 (MQ=255)
                                                   gATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCATAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGATGATTTACTGCTGCTTATCTGTAGAAAAGGTATCAGGtgtg                                                                                          <  2:1059642/140‑4 (MQ=255)
                                                    aTTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGc                                                                                         >  1:2820935/1‑140 (MQ=255)
                                                       aTCCAGATATGGAATTCGTAATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGAAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTg                                                                                      <  2:4152973/140‑1 (MQ=255)
                                                           aGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTg                                                                                  <  1:3336206/140‑1 (MQ=255)
                                                           aGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTg                                                                                  <  1:3447406/140‑1 (MQ=255)
                                                           aGATATAGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTg                                                                                  <  1:744242/140‑1 (MQ=255)
                                                            gATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGc                                                                                 <  2:2743566/140‑1 (MQ=255)
                                                             atatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCt                                                                                >  2:719207/1‑140 (MQ=255)
                                                              tatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCtt                                                                               >  1:2014533/1‑140 (MQ=255)
                                                                  gAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCg                                                                                        <  1:3757209/127‑1 (MQ=255)
                                                                  gAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCtt                                                                           >  2:398484/1‑140 (MQ=255)
                                                                     ttCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGACTGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTAc                                                                        >  2:3557746/1‑140 (MQ=255)
                                                                       cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCAATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATATGTCGAAAACGAATCAGGTCGGCGTGATTGATTCCTTTACGt                                                                      >  1:1309086/1‑140 (MQ=255)
                                                                         tCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTgg                                                                    <  1:4221393/140‑1 (MQ=255)
                                                                             ttCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGc                                                                <  1:2189196/140‑1 (MQ=255)
                                                                                tAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCgg                                                             <  1:1279818/140‑1 (MQ=255)
                                                                                 aaTGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGa                                                            <  1:2496286/140‑1 (MQ=255)
                                                                                  aTGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAg                                                           >  2:377672/1‑140 (MQ=255)
                                                                                   tGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTg                                                                                  >  1:38349/1‑116 (MQ=255)
                                                                                     cTAAAGCAAAAGCTGATACTGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTa                                                        <  1:3422108/140‑1 (MQ=255)
                                                                                     cTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTa                                                        <  1:1798698/140‑1 (MQ=255)
                                                                                      tAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAg                                                       <  1:2341537/140‑1 (MQ=255)
                                                                                      tAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAg                                                       <  1:2736222/140‑1 (MQ=255)
                                                                                      tAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAg                                                       <  2:2308435/140‑1 (MQ=255)
                                                                                       aaaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      >  2:1490117/1‑140 (MQ=255)
                                                                                       aaaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      <  2:2592723/140‑1 (MQ=255)
                                                                                       aaaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      <  2:3433234/140‑1 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     >  1:1220342/1‑140 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     >  2:1576508/1‑140 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     <  1:449489/140‑1 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     >  2:3450945/1‑140 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     >  2:3173733/1‑140 (MQ=255)
                                                                                           cAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGt                                                  <  2:803790/140‑1 (MQ=255)
                                                                                                    aTAATTTGACGAGTTTACATTTCAGCGGCGAAGCAATTGGTTTTTTTTGAGGAATTAAATCTGCCACTCTGTCGAAAACGAATCATGTCGGAGTGATTTCTTCATATAAGTTGAAGCAGGTTGTAGACTGTTTTATCaaa                                         >  2:3829476/1‑140 (MQ=255)
                                                                                                    aTAATGTGACGAGTGTCCATTTCAGCGGCGACGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGTATGAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTCTCCGGTTTTATCaag                                         >  1:967112/1‑139 (MQ=255)
                                                                                                    aTAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      >  1:315629/1‑127 (MQ=255)
                                                                                                    aTAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACg                                                    <  2:319317/129‑1 (MQ=255)
                                                                                                    aTAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGACAGCCGGAGGTAGACGGTTTTATCaaa                                         >  2:1617986/1‑140 (MQ=255)
                                                                                                    aTAATGAGACGAGGGTCCATTTCAGCTGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGATGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTGACGTGGCAGCCGGAGGTAGACGGTTTTATCaaa                                         <  2:2251751/140‑1 (MQ=255)
                                                                                                          tgACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      >  2:1908080/1‑121 (MQ=255)
                                                                                                          tgACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      >  2:1191603/1‑121 (MQ=255)
                                                                                                          tgACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      <  1:1908080/121‑1 (MQ=255)
                                                                                                          tgACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGcc                                   >  2:581346/1‑140 (MQ=255)
                                                                                                           gACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCa                                  >  2:1289330/1‑140 (MQ=255)
                                                                                                            aCGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGACTCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaa                                 <  2:1100681/140‑1 (MQ=255)
                                                                                                            aCGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaa                                 >  1:3046077/1‑140 (MQ=255)
                                                                                                            aCGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaa                                 >  2:373472/1‑140 (MQ=255)
                                                                                                              gAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaatt                               >  2:3375754/1‑138 (MQ=255)
                                                                                                                   tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                          >  1:898137/1‑140 (MQ=255)
                                                                                                                   tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                          >  1:4150039/1‑140 (MQ=255)
                                                                                                                   tCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCCAATATGaa                          >  2:60431/1‑140 (MQ=255)
                                                                                                                   tCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                          >  2:3263598/1‑140 (MQ=255)
                                                                                                                   tCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                          >  1:291254/1‑140 (MQ=255)
                                                                                                                   tCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaa                          >  1:2184168/1‑140 (MQ=255)
                                                                                                                      aTTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                       >  2:4000362/1‑140 (MQ=255)
                                                                                                                      aTTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                       <  2:139679/140‑1 (MQ=255)
                                                                                                                      aTTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                       >  2:3539031/1‑140 (MQ=255)
                                                                                                                        ttCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATcc                     <  2:965869/140‑1 (MQ=255)
                                                                                                                        ttCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATcc                     <  1:1632213/140‑1 (MQ=255)
                                                                                                                           aGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTc                                                                              >  1:1072357/1‑80 (MQ=255)
                                                                                                                             cggcgAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGata                >  2:173204/1‑140 (MQ=255)
                                                                                                                             cggcgAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGata                >  2:45046/1‑140 (MQ=255)
                                                                                                                                       aTGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCaa      <  2:2184168/140‑1 (MQ=255)
                                                                                                                                        tGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac     <  1:3986221/140‑1 (MQ=255)
                                                                                                                                        tGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac     <  1:3121225/140‑1 (MQ=255)
                                                                                                                                           gTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGGCAAATATGAAAATCCGGATATAGAAGTCAAcaca  <  2:389851/140‑1 (MQ=255)
                                                                                                                                           gTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAcaca  <  2:229635/140‑1 (MQ=255)
                                                                                                                                           gTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAcaca  <  1:45046/140‑1 (MQ=255)
                                                                                                                                           gTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAcaca  <  1:173204/140‑1 (MQ=255)
                                                                                                                                           gTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAcaca  <  1:1194478/140‑1 (MQ=255)
                                                                                                                                           gTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAcaca  <  2:839759/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTCTATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAACACA  >  NZ_CP034943/1176034‑1176312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: