Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,366,028 A→C 7.0% T176P (ACG→CCG)  EO946_RS07135 → PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,366,0280AC 7.0% 527.7 / 3.8 189T176P (ACG→CCG) EO946_RS07135PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (79/88);  new base C (2/19);  total (81/108)
Fisher's exact test for biased strand distribution p-value = 7.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

AAAGAAGATGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTG  >  NZ_CP034943/1365890‑1366163
                                                                                                                                          |                                                                                                                                       
aaaGAAGATGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAc                                                                                                                                        >  2:3386030/1‑140 (MQ=255)
aaaGAAGATGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAc                                                                                                                                        >  2:4243210/1‑140 (MQ=255)
   gaagaTGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAcggg                                                                                                                                     <  1:2814088/140‑1 (MQ=255)
    aagaTGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAcgggc                                                                                                                                    >  1:4160883/1‑140 (MQ=255)
     agaTGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAcgggcg                                                                                                                                   <  1:1661804/140‑1 (MQ=255)
          gagaAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACa                                                                                                                              >  2:3542794/1‑140 (MQ=255)
              aaCATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCgc                                                                                                                          >  2:501990/1‑139 (MQ=255)
               aCATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGa                                                                                                                         <  1:2994360/140‑1 (MQ=255)
               aCATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGa                                                                                                                         <  1:3746204/140‑1 (MQ=255)
                 aTACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATg                                                                                                                       >  2:1564513/1‑140 (MQ=255)
                       aGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAg                                                                                                                 >  1:2004842/1‑140 (MQ=255)
                       aGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAg                                                                                                                 >  1:1168962/1‑140 (MQ=255)
                           aaGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGAtct                                                                                                             <  2:165587/140‑1 (MQ=255)
                                aGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGa                                                                                                                                <  1:3373261/116‑1 (MQ=255)
                                aGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGa                                                                                                                                >  2:3373261/1‑116 (MQ=255)
                                aGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGaaa                                                                                                        <  1:3699666/140‑1 (MQ=255)
                                 gACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGaaaa                                                                                                       >  1:172489/1‑140 (MQ=255)
                                 gACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGaaaa                                                                                                       >  1:661847/1‑140 (MQ=255)
                                  aCATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACAGATTACGAAAAAAGAAGCCAAAACCATTTCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGaaaaa                                                                                                      >  1:4089577/1‑140 (MQ=255)
                                       aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTgg                                                                                                 >  1:3247036/1‑139 (MQ=255)
                                       aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGa                                                                                                 >  1:3734003/1‑140 (MQ=255)
                                       aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGa                                                                                                 >  2:221739/1‑140 (MQ=255)
                                       aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGa                                                                                                 >  2:3731875/1‑140 (MQ=255)
                                       aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAACTGa                                                                                                 >  1:913532/1‑140 (MQ=255)
                                       aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATAACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGa                                                                                                 >  2:1079965/1‑140 (MQ=255)
                                        aTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAg                                                                                                >  1:2250557/1‑140 (MQ=255)
                                        aTGCAAAAGCAGTCGTGTCAGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAg                                                                                                >  1:1160518/1‑140 (MQ=255)
                                         tGCACAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAACACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAgg                                                                                               <  1:1607266/140‑1 (MQ=255)
                                         tGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAgg                                                                                               <  2:804956/140‑1 (MQ=255)
                                            aaaaGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCa                                                                                            <  1:15567/140‑1 (MQ=255)
                                            aaaaGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCa                                                                                            <  1:2214418/140‑1 (MQ=255)
                                              aaGCAGTCGTGTCTGCTGATCGAATCCGCGGTGAAGATCAACAAAAAACACCGAGTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCAct                                                                                          >  1:3550793/1‑140 (MQ=255)
                                                gCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAACACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCActct                                                                                        <  1:209675/140‑1 (MQ=255)
                                                gCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCActct                                                                                        <  1:2771505/140‑1 (MQ=255)
                                                gCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCActct                                                                                        <  1:981715/140‑1 (MQ=255)
                                                gCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCActct                                                                                        <  1:450453/140‑1 (MQ=255)
                                                   gTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGAtct                                                                                                             <  2:393623/116‑1 (MQ=255)
                                                   gTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGAtct                                                                                                             >  1:2563317/1‑116 (MQ=255)
                                                    tCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATc                                                                                    >  2:1862859/1‑140 (MQ=255)
                                                    tCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATc                                                                                    >  2:3762828/1‑140 (MQ=255)
                                                       tgtCTGCTGATCGAATCAGCGGTGAAGATCAACAAACAACACCGATTACGAAAAAAGAAGCCAAAACCAGTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCttt                                                                                 <  1:3870772/140‑1 (MQ=255)
                                                       tgtCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCttt                                                                                 <  1:4257933/140‑1 (MQ=255)
                                                            gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAACAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  2:455466/140‑1 (MQ=255)
                                                            gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAACACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  1:181377/140‑1 (MQ=255)
                                                            gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGGGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  2:1764767/140‑1 (MQ=255)
                                                            gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  1:586066/140‑1 (MQ=255)
                                                            gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  2:1937401/140‑1 (MQ=255)
                                                            gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCAAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  1:3104787/140‑1 (MQ=255)
                                                                aTCGACTCAGCGGTGAAGATCAACAAAAAACACCGAGTACGACAAATGAAGCCAAAACCAGTGCTGAGCGAGAACCGGGCGGGACAGCGGAGGAGGCAGAGCTCGAAAAATGTGAGGGCCCTCTCATCTGTGAAGTGGag                                                                        >  1:2510752/1‑139 (MQ=255)
                                                                aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  1:1126750/1‑140 (MQ=255)
                                                                aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  1:3047352/1‑140 (MQ=255)
                                                                aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  1:62938/1‑140 (MQ=255)
                                                                aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  1:528680/1‑140 (MQ=255)
                                                                aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  1:3944500/1‑140 (MQ=255)
                                                                aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  1:2052727/1‑140 (MQ=255)
                                                                   gAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAACCGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGtt                                                                     <  1:241681/140‑1 (MQ=255)
                                                                     aTCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGa                                                                   >  1:1222705/1‑140 (MQ=255)
                                                                       cAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATc                                                                 <  2:3990722/140‑1 (MQ=255)
                                                                            gTGAAGATCAACAAAAAACCCCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCg                                                            <  1:3815925/140‑1 (MQ=255)
                                                                            gTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCg                                                            <  1:213001/140‑1 (MQ=255)
                                                                              gAAGATCAACAAAACACACCGATTACGAAAAAAGAAGCCAAACCCATGGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCACATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  1:3424579/140‑1 (MQ=255)
                                                                              gAAGATCAACAAAAACCACCGATTACGAACAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  1:3517682/140‑1 (MQ=255)
                                                                              gAAGATCAACAAAAAACCCCGATTACGCAAACTGCAGCCAAAACCCTTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAACACTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  2:66416/140‑1 (MQ=255)
                                                                              gAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  2:3174543/140‑1 (MQ=255)
                                                                              gAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  1:2943217/140‑1 (MQ=255)
                                                                              gAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  2:1969408/140‑1 (MQ=255)
                                                                               aaGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCAct                                                                                          >  2:608700/1‑107 (MQ=255)
                                                                               aaGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGgat                                                         >  2:3500931/1‑140 (MQ=255)
                                                                                    caacaaAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGgatg                                                        >  2:657355/1‑136 (MQ=255)
                                                                                      acaaAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAaag                                                  >  2:2852675/1‑140 (MQ=255)
                                                                                       caaAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAaaga                                                 >  2:2522289/1‑140 (MQ=255)
                                                                                       caaAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAaaga                                                 >  2:1114142/1‑140 (MQ=255)
                                                                                       caaAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAaaga                                                 >  1:905989/1‑140 (MQ=255)
                                                                                            aaCACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGa                                            >  1:13257/1‑140 (MQ=255)
                                                                                                   aTTACTAAAAAACAACCCAAAACCTTTGCTGAGCGATAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGAACTCTCATATTTGAAGTGGATGTTGATCTTCCGTATTATAAAGAAGCGACTGTGaa                                     <  2:4089577/140‑1 (MQ=255)
                                                                                                       ctaaaaaaCAAGCAAAAACCTTTGCTGCGCGATAACGGGGCGGGACAGCGGATGACGCATATCTCTAAAAAAGTGTGGGCACTCTAATCTTTTAAGTGTTTTTTGATGTTCCGTATGATAAAGAAGCGACTGTGAAAAtt                                 <  2:2397987/138‑1 (MQ=255)
                                                                                                       cGAAACAAGAACCCAAACCCTTTGCTGCGCGAGAAACGGGCGGGACAGGGGATGACTCAGATATCGAAAAAAGTGAGGGCACTATAATGTTTGATTTGGTTGTTGATCTTCCGCATGATAAACACGCGACTGTGAAAAtt                                 <  2:2101179/140‑1 (MQ=255)
                                                                                                       cGAAAAAACAACCAAAACCCAGTGCTCCGCGAGAACCGGGCGGGACTGCGGATGACCCAGATCTCTAAAAAAGTGAGGGCACTCTCATATTTGAAGTGTTTGTTGATCTTCCGGATCATAAAGAAGCGACTGTGAAAAtt                                 <  2:3047352/140‑1 (MQ=255)
                                                                                                             attaagCCAAAACCATTCCTGCGCGCCAAAGGGGCGGTACATGGTCTGACGCACATGTTCAAAAAATTGCTGGCACTATAATTTTTTAAGTGGATGTTTATATACCGGATGATATTTAAGCGACTGTGAAAATTAATGcc                           <  2:1010176/137‑1 (MQ=255)
                                                                                                                  gCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGa                                                                                                                         <  2:1463121/41‑1 (MQ=255)
                                                                                                                  gCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGa                                                                                                                         >  1:1463121/1‑41 (MQ=255)
                                                                                                                       aaCCTTTGCTGAGCTACAACCGGGCGGGACAGCGGTTGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGGGGTTGTTGATCTTCGGGATTATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGaa                 <  2:1396474/140‑1 (MQ=255)
                                                                                                                       aaCCATGGCTGAGCTAAAAACGGGCGGGACAGCGGATGACGCTGATCTCGAAAAAAGTGAGGGAACTATCATCTTTTATGTGTATGTTTATCTTCGGGATCATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGaa                 <  2:3676870/140‑1 (MQ=255)
                                                                                                                       aaCCATGGCTGAGCGAGAAACGGGCGGGACAGCGGATCACGCAGATATCTAAAAAAGTGAGGGCACTATAATATTTGAAGTGCATGTTTTTCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGaa                 <  2:3413620/140‑1 (MQ=255)
                                                                                                                         cctgtGCTGAGGGAGAACCGGGCGTGACTGCGTATCACGATTATCTCGAAAAAAGTGAGGGCACTCTAATTTTTTAAGTGTATTTTTATCTTCCGGATGATAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAg               <  2:2695039/136‑1 (MQ=255)
                                                                                                                           cttGCTGAGCGACAAACGGGCGGGACAGCGGATGACCCAGATCTCCAAAACAGTGAGGGCACTCTCATATTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGt             <  2:3133483/139‑1 (MQ=255)
                                                                                                                            ttGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGtt            >  2:2742054/1‑140 (MQ=255)
                                                                                                                            ttGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGtt            >  2:1866511/1‑140 (MQ=255)
                                                                                                                             tGCTTAGCGAGAAAAGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGGTGATCTGCCGGATGATAAAGAAGCGACTGTGAAAATTAATGACTACAACGGAAAGGTTg           >  2:3710012/1‑140 (MQ=255)
                                                                                                                               cttaGCGATAAACGGGCTGCACCGGGGTTGACTCAGATCTCCAAAAATGTGAGGCCAATATAATCTTTTAAGTGCTTGTTGATGTTCCGGATGATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  1:960552/137‑1 (MQ=255)
                                                                                                                               cttaGCCAGAACCGGGCGGGACCGGGGATGACGCAGATCTCTAAAAACGTGAGGGCACTCTCATGTTTCAAGTGTATGTTGATCTTCCGCTTTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  2:254744/137‑1 (MQ=255)
                                                                                                                               cTGAGCTACAACCGGGCGGGACCGCGGTTGACGCATATCTCTAAAAAAGTGAGGCCACTCTAATCTTTGATGTGTATGTTGATCTTCCGTATGATAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  1:3056687/140‑1 (MQ=255)
                                                                                                                               cTGAGCGAGAACCGGGCGGGACCGGGGATGACGCTTATCTCTAAAAAAGTGAGGCCACTCTCATCTTTTAAGGGGTTGTTTATGTTCGGGATTATAAATACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  2:536407/140‑1 (MQ=255)
                                                                                                                                  aGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAAc      >  1:2696526/1‑140 (MQ=255)
                                                                                                                                   gctagaAACGGGCGGGACCGCGTATGACGCATATCTCTAAAAAAGTGAGGGCACTATAATATTTCAAGTGTATGTTTATCTTCCGTATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  2:172489/137‑1 (MQ=255)
                                                                                                                                   gCGAGAACCGGGCGGCACAGCGGATGACGCAGACCTCCAAAAACGTGAGGGCACTCTAAACTTTGAGGTGGTTGTTTATCTGCCGGTTTATAAACCAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  2:3564223/140‑1 (MQ=255)
                                                                                                                                    cGAGAAACGGGCGTCACTGCGGATGCCGAATTTCTCGAAAAAAGTGAGGGAACTATCATATTTTAAGTGGATGTTGCTCTTCCGGATGATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACAt    <  2:2004842/140‑1 (MQ=255)
                                                                                                                                    cGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGCCACTCTCATCTTTGAAGTGTTTGTTGATCTTCCGGATTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACAt    <  2:1168962/140‑1 (MQ=255)
                                                                                                                                      agaAACGGGCGGTACCGCGTCTGACGCAGATCTCTAAAAAATTGAGGGAACTATCATCTTTGAAGTGGATGTTGATCTTCCGTATGAAAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATtg  <  2:3200222/140‑1 (MQ=255)
                                                                                                                                        aaaCGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCa                                                                                            <  1:1114009/48‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
AAAGAAGATGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTG  >  NZ_CP034943/1365890‑1366163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: