Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,979,248 T→C 26.1% noncoding (1553/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,979,2480TC26.1% ‑3.0 / 16.0 31noncoding (1553/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (6/16);  new base C (2/6);  total (8/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCAT  >  NZ_CP034943/2979114‑2979384
                                                                                                                                      |                                                                                                                                        
cttatcaTTCTCTCCTCGAATACATTAATCGGAATGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGaa                                                                                                                                     >  2:2093731/7‑140 (MQ=1)
ctgaGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGaa                                                                                                                                     <  1:2579728/137‑1 (MQ=1)
ctgaGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGaa                                                                                                                                     <  2:3458505/137‑1 (MQ=1)
          tcacctcGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGc                                                                                                                           >  2:2900688/4‑140 (MQ=1)
          taaactagCCTACCTGTGTCTGTTTTAGTTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGc                                                                                                                           >  2:1555279/8‑140 (MQ=1)
          taaactagCCTACCTGTGTCGGTTTTAGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGc                                                                                                                           >  2:409794/8‑140 (MQ=0)
                cggcTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGATACTACATTTCTCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTAttt                                                                                                                     <  2:2476114/137‑1 (MQ=0)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTATATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                   <  1:1581079/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                   <  1:2412477/137‑1 (MQ=1)
                  ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                   <  1:1333322/137‑1 (MQ=1)
                    gacccgggtCGGCTTGCGGGACGGGCACCTCTCACCCCGCTAGAGGCTGTTCTTGGCAGTGTGGAATCAGGAACGTCGCTACGACATTTCGCACGCCATCACAGCTCACCCGTATGGGAAACGGATTTGCCTATTTCCCa                                                                                                                 <  2:306115/133‑1 (MQ=1)
                        tgggtCTGTTTGCGGTACGGGCACCTCTCACATCGCTATAGGGTTTTATTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCAGAGCTCAGGCTTAGGGGTAACGGATTTGCCTATTTCGCAGCCt                                                                                                             <  2:1925990/137‑1 (MQ=1)
                                        accggCACCACTCACCTCGCTAGAGGCATTTCGTGGCAGTGTGGAATCATGAACGTCGCTAATACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCgg                                                                                             <  2:3289494/137‑1 (MQ=1)
                                        aaggtcACCTCTCACCTCGCTAGAGGCTTTTCTTTGCAGTGTGTAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCATTATGCGAAACGGATTTGACTATTTCCCAGCCTAACTGCTTGGACGCgg                                                                                             <  2:2333035/135‑1 (MQ=1)
                                                 taaaacatCGCTAGACGATTTTCTTTACAGACAGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAAt                                                                                    >  2:4079384/8‑140 (MQ=1)
                                                         cgttAGAGGCTTTTATTGTCAGTGTGTAATCAGGAACTTCGCTACTAAATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGATTGGACTCGGATATCCAATACCGCGCt                                                                            <  1:3066871/137‑1 (MQ=1)
                                                         cgatAGAGGCTTTTCTTGTCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCACAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCt                                                                            <  2:993698/137‑1 (MQ=0)
                                                         cgatAGAGGCTTTTCTTGGCAGTGTTGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATTGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCt                                                                            <  2:1610450/137‑1 (MQ=0)
                                                                 gcgtttCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTAt                                                                    >  1:1714358/4‑140 (MQ=1)
                                                                          ggcacggtgGAATCAGGAACTACGCTACGACAGTTCGCCCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTAGGACGCGGAGATCCAATACCGCGCGTACCCTATCCTCCTGCg                                                           <  2:1170160/134‑1 (MQ=1)
                                                                                 tgtaaTCAGTAAATTACCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTcccccc                                                    >  2:3735591/4‑140 (MQ=1)
                                                                                  ggcaTCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAAGACCGCGCTTACCCTATCCTCCTGCGTCCCCCCa                                                   <  1:1715044/137‑1 (MQ=0)
                                                                                                 gcgactacATTACGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCAATTTCCCAGCCTAACTGCTAGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTga                                    <  1:559251/137‑1 (MQ=1)
                                                                                                      gacttttCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATGTGCCTATTTCCCAGCCTCACTGCTTGGACGCGGATATCCAAGACCGCGCTTCCCCTATCCTCCTGCGTCCCCCCCTTGCTCAAATGGTGaggagg                               <  2:4181339/136‑1 (MQ=1)
                                                                                                        cacttCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCgcgc                                                                             >  1:2376481/4‑92 (MQ=1)
                                                                                                        cacttCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCgcgc                                                                             <  2:2376481/89‑1 (MQ=1)
                                                                                                         atgtcgctcgcCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAggtgg                            <  2:998789/137‑1 (MQ=1)
                                                                                                              cctagcCATCACAGCTCAGCCTGATGGGAATCGGATTTGCCTATGTCCCAGCCTAACTGCTTGGACGCGGCTCTCCAAGACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAg                       <  1:181350/136‑1 (MQ=1)
                                                                                                                            ctgaGCCTTATGTTAAACTGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATACAATACCGCGCTTACCCTATCCTCCGGCGTCCCCCGATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCtgt         <  1:3323702/137‑1 (MQ=1)
                                                                                                                                  ctgaTGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCa   <  1:2503709/137‑1 (MQ=1)
                                                                                                                                  ctgaTGGGAAACGGATTGGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATAAACCTGTTGTCCa   <  2:2411152/137‑1 (MQ=0)
                                                                                                                                   ttctGGGACACGGTTGTGCCTATTTCCCAGCCTAACTGCTTGGACGCTGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCAt  <  1:4217599/137‑1 (MQ=1)
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CTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCAT  >  NZ_CP034943/2979114‑2979384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑