| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_CP034943 | 2,980,160 | G→A | 25.0% | noncoding (641/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 2,980,160 | 0 | G | A | 25.0% | ‑2.7 / 14.8 | 28 | noncoding (641/2928 nt) | EO946_RS15430 | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (6/15); new base A (2/5); total (8/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC > NZ_CP034943/2980021‑2980296 | cgcgCCTCCATTCAGTGTGACCTGCACTTCACCCTGGACATGGGTAGATCACCAGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTAGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACg < 1:4026767/137‑1 (MQ=1) ccgccATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGAt < 2:263124/137‑1 (MQ=1) agtctaTTCTGGGTTCCCTGAACTTCAGCCTGGACATGGGTAGATCACCTGGTTTCGGGGCTACGACCACGTAGTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTGCGCATCTTCTGCTTAACCTTGCACGGGAt < 2:2344889/135‑1 (MQ=1) tggtACCTGAACTTCACCCTGGACATGGGTAGATCACGTGGGTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCTCTTTCGCTGAGGCTCCGCATCTTCTGCTTAACCTTGCACGGGAACGTAACTctac > 2:1910845/4‑137 (MQ=1) tgatACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGcc > 1:2277455/4‑140 (MQ=1) gtgaCCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCg < 2:3348333/137‑1 (MQ=1) ccagAACTTCACCCTGGACATGGGTAGATCACCTGGTTACGGGTCTACGAACACGTACTCATGCGCCCTATTAAGCCTCGCTTTCGCTCCGGATCCGAATCTTCTGCATAACCTTGCACGCGAACCTAACTCGCCGGtaa > 2:1525681/4‑138 (MQ=1) tgaccgtCACCCAGGACATGGGTAGATCCCCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCAt < 1:956011/134‑1 (MQ=0) cgacctcggTAGATCACCTGCTTTCGGGTCTACGACCACGTACTCATGCGCCCTCTTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCa < 1:2440279/133‑1 (MQ=1) ggctAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTAGTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCAt < 1:2190943/137‑1 (MQ=1) ccgggTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTGTCGCTGCGGCGCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTaa > 2:1037695/4‑140 (MQ=0) cttgTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAAGCTTTCACGGGATCGTAACTCGCCGGTTCTTTCTACAAAAGGCACGCCATCACCCGTTAAc > 2:3484582/4‑140 (MQ=0) cttgTTTCGGGTCTACGACCACGAACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCGCCGCCTCTTCTGCGTAACCTTGCTCGGGATCGTAACTCGCCGGGTCATGCTACACAAGGCACGCCATCACCCGTTAAc > 2:1581608/4‑140 (MQ=1) gtgtCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACggg < 2:4012102/137‑1 (MQ=1) ggcgcgTCCACGACCACGGACACAGGCGCCCGCGTCAGCCTCGCTTGCGCGGCGGCTCCGCATCTTCTGCTGAACCGTGCACGGGATAGTACCTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGct < 2:2417679/135‑1 (MQ=1) gtataagACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGac < 1:453590/134‑1 (MQ=1) attcgcCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACGCGCCGGTGCATTCTACAAAAGGCACGCCATCACCCGTTAACGGCCGCTGCCTAGTTGTCGGCACACGGt > 1:2417120/4‑140 (MQ=1) cctaggcAGACTCGCTGTCGCTGCGGCTCCGCATCTTCGGCTTACCCTGGCACACGATCGGAACCCGCCGGTTCATTCTACACAAGGCACCCCATCACCCGTGAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAgg < 2:139863/134‑1 (MQ=1) tagtCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGAt < 1:3787249/137‑1 (MQ=1) tctctgtCGATGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCTTAACTCGCCGGTTCATTCTAAAAAAGGCACGCCATCACCGGTTAACGGGATCTGACTTGTTTTAGGCACACGGTTTGAGGATCTCTTTCAc < 2:266469/134‑1 (MQ=1) gcgttCGCTGCGGCTCCGCATCTTCTGCTTACCCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTc < 2:1400864/137‑1 (MQ=0) gcgttCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTc < 1:218598/137‑1 (MQ=1) gcgttCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTc < 2:1470350/137‑1 (MQ=1) cgtcTCCGGATCTTCTGATTAACATTGCACGGGATCGTAACTCGCAGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTAATTCTAGGCACACGGTTTCAGTATCTCTTTCAATCCCCTTCCggg < 2:1673394/137‑1 (MQ=1) gggtCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGGTCATTCTACAAAAGGGACGCCATCTCCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCgggg < 2:2843721/137‑1 (MQ=0) cagcttctGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCgttt > 1:1895599/4‑140 (MQ=0) cttcTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACCAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCtt < 2:866169/137‑1 (MQ=1) acattGCACGGGATCGTAACTGGCCGGTTCATTGTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTAGTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCa < 1:2901440/137‑1 (MQ=0) acattGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCAATACACTTCCGGGGTGCTTTTCACCTGTCCCTCa < 2:838116/137‑1 (MQ=1) catgggATCGTAACTCGCCGGTGCATTCTACATAAGGCACGCCATCACCCGTTAACGGGCTATGAATACTTTTAGTCACACGGTTTCAGGATCTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAc < 2:3588353/137‑1 (MQ=1) | CGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC > NZ_CP034943/2980021‑2980296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |