Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,184,761 T→G 5.4% R122R (AGG→CGG)  EO946_RS16485 ← tautomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,184,7610TG 5.4% 755.7 / 6.4 294R122R (AGG→CGG) EO946_RS16485tautomerase family protein
Reads supporting (aligned to +/- strand):  ref base T (140/127);  new base G (2/25);  total (142/152)
Fisher's exact test for biased strand distribution p-value = 2.99e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

TCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAC  >  NZ_CP034943/3184628‑3184900
                                                                                                                                     |                                                                                                                                           
tcaaactatcaaacAAGAAAACGGGAGAACAATTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCAtt                                                                                                                                       >  2:740035/1‑140 (MQ=255)
       atcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCCATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGaaa                                                                                                                                >  2:3454736/1‑140 (MQ=255)
       atcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGaaa                                                                                                                                >  2:3337476/1‑140 (MQ=255)
          aaacACGAACACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCAGCTGGGCCCGTCCATTTCCGAAAGAc                                                                                                                             <  2:1251344/140‑1 (MQ=255)
          aaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAc                                                                                                                             <  1:1550034/140‑1 (MQ=255)
          aaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAc                                                                                                                             >  1:1783744/1‑140 (MQ=255)
           aacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCGTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGATTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAcc                                                                                                                            <  1:2813217/140‑1 (MQ=255)
                gAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATcc                                                                                                                       >  2:3321318/1‑140 (MQ=255)
                gAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATcc                                                                                                                       >  2:1743270/1‑140 (MQ=255)
                            aCAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGAttt                                                                                                           <  1:4169389/140‑1 (MQ=255)
                                 tAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGc                                                                                                      <  2:5027/140‑1 (MQ=255)
                                 tAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCAATTAAATTTACTATATAACAATTCTCATACTGAtttagtgg                                                                                                      <  2:3135578/140‑6 (MQ=255)
                                     atagaAACAGGCACAAGGCGTTGAATCAACTTCCCATTAATAGACCACCACTCGGGTATCAATCCTCGGGTTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGc                                                                                                  <  2:2511412/138‑1 (MQ=255)
                                          aaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCCATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGAt                                                                                             >  1:1718323/1‑140 (MQ=255)
                                          aaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGAt                                                                                             >  1:3032841/1‑140 (MQ=255)
                                          aaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGAt                                                                                             >  1:930550/1‑140 (MQ=255)
                                           aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATa                                                                                            >  2:2812668/1‑140 (MQ=255)
                                           aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATa                                                                                            <  1:2015080/140‑1 (MQ=255)
                                                cacaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAacac                                                                                       <  1:859915/140‑1 (MQ=255)
                                                cacaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                                                                  >  2:3867277/1‑97 (MQ=255)
                                                cacaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAacac                                                                                       <  1:1141522/140‑1 (MQ=255)
                                                cacaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAacac                                                                                       <  1:975655/140‑1 (MQ=255)
                                                 acaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAACGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAAcaca                                                                                      <  1:2951806/140‑1 (MQ=255)
                                                          tGAATCACCTTCCCATTAATAGACCACCCCGCGGGTCTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttctttt                                                                             >  2:1533766/1‑140 (MQ=255)
                                                          tGAATCACCTTCCCATTAATAGACCACCCCGCGGGTCTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttcgttt                                                                             >  1:3728707/1‑140 (MQ=255)
                                                          tGAATCACCTTCCCATTAATAGACCACCCCGCGGGTCTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACAACttctttt                                                                             >  2:2620651/1‑140 (MQ=255)
                                                          tGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCACTCCTCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttctttt                                                                             >  2:406113/1‑140 (MQ=255)
                                                          tGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCAGCCTGATTTCCGGCCAGCATGATAAACACATCttctttt                                                                             >  1:2876807/1‑140 (MQ=255)
                                                             aTCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttcttttctt                                                                          >  1:1447682/1‑140 (MQ=255)
                                                             aTCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCCTCCTGATTTCCGGCCAGCATGATAAACACATCttcttttctt                                                                          <  1:59600/140‑1 (MQ=255)
                                                             aTCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttcttttctt                                                                          >  2:811403/1‑140 (MQ=255)
                                                                aCCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACg                                                                       >  1:368656/1‑140 (MQ=255)
                                                                 ccTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTATTAAGc                                                                      >  1:3963536/1‑140 (MQ=255)
                                                                  cTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGcc                                                                     <  2:3032841/140‑1 (MQ=255)
                                                                       cATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCt                                                                <  1:1931710/140‑1 (MQ=255)
                                                                       cATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCt                                                                <  1:3184800/140‑1 (MQ=255)
                                                                           actAGACCACCCCGCGGGGATCAATCCGCGGGGTTTTATTATTCAATCCTCTGGGCCCTTCCATGTCCGAACGACCAATCCGCAGCCTGATGTCCGGCCAGCAGGAGAACCCCATCTTCTTTTCTTACGCCAAGCTCGga                                                            <  2:838444/138‑1 (MQ=255)
                                                                            aTAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCg                                                                                                                                   >  2:2580070/1‑68 (MQ=255)
                                                                            aTAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCg                                                                                                                                   <  1:2580070/68‑1 (MQ=255)
                                                                               gACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatga                                                        >  1:1025120/1‑140 (MQ=255)
                                                                               gACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatga                                                        >  1:2146612/1‑140 (MQ=255)
                                                                                accaccCCGCGGGTCTCCAGCCGCGGGGTGTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAAGCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCGTCTTTTCTTACGCCACGCTCGgactgaa                                                       >  1:3918590/1‑140 (MQ=255)
                                                                                accaccCCGCGGGTATCACTCCGCCGGGTTTTATTAGTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCCTCCTGATGACCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatgaa                                                       >  2:778788/1‑140 (MQ=255)
                                                                                accaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCGATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCAAGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatgaa                                                       >  2:4079338/1‑140 (MQ=255)
                                                                                accaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatgaa                                                       >  1:1753486/1‑140 (MQ=255)
                                                                                accaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTGTCTTACGCCAAGCTCGgaatgaa                                                       >  2:2027603/1‑140 (MQ=255)
                                                                                accaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGACCTTCCATTTCAGAAAGACCAATCCTCATCCTGATTTCCGGCGAGCATGATAAACTCATCTTCTTTTCTTACGCCAAGCTCGgaatgta                                                       >  2:2976355/1‑138 (MQ=255)
                                                                                accaccCCGCGGGTATCAATCAGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTAAGCCAAGCTCGgaatgaa                                                       >  1:2378062/1‑140 (MQ=255)
                                                                                accacaCCGCGAGTATCACTCCCCGGGGTATTATAATTCAAAAATCAGGGCCCTTCCATTTCAGAAAGACAAATACTCATCCTGATATCCGGCCAGCTTGATAAAAACATATTCTTTTCTTACGCCAAGCTCGgaatgaa                                                       >  2:96916/1‑140 (MQ=255)
                                                                                 ccaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatgaat                                                      <  2:3608092/140‑1 (MQ=255)
                                                                                  ccccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATc                                                     <  1:2292326/138‑1 (MQ=255)
                                                                                    ccccGCGGGTATCCATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTACGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCGTTTCTTACGCCAAGCTCGGAATGAATCCg                                                   >  1:2720109/1‑140 (MQ=255)
                                                                                    ccccGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                                                   >  1:1669821/1‑140 (MQ=255)
                                                                                    ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACTCCAAGCTCGGAATGAATCCg                                                   >  1:2218170/1‑140 (MQ=255)
                                                                                    ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGCATGAATCCg                                                   >  2:3561372/1‑140 (MQ=255)
                                                                                    ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                                                   >  2:717615/1‑140 (MQ=255)
                                                                                    ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCCATCCTCATCCTGATTTCCGGCCAGCCTGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGACTCCg                                                   >  1:487336/1‑140 (MQ=255)
                                                                                     cccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCgg                                                  >  1:3487704/1‑140 (MQ=255)
                                                                                       cgcgGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCCGCATGATAAACACAACTTCGTTTCTTACGCCAAGCTCGGAATGAATCCGGCt                                                >  1:4091984/1‑140 (MQ=255)
                                                                                       cgcgGGTATCAATCCGCGGGGTTTTAGTATTCAATCATCTGGGCCCGTCCATGTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACCTCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCt                                                <  2:4248369/140‑1 (MQ=255)
                                                                                            gTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGa                                           <  2:609502/140‑1 (MQ=255)
                                                                                              aTCAATCCGCGGGGTTTTATTATTCACTCATCTGGGCCCTTCCATTTCCGAACGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAACCTCGGAATGAATCCGGCTTGCGAtt                                         <  1:1592916/140‑1 (MQ=255)
                                                                                              aTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt                                         <  1:565154/140‑1 (MQ=255)
                                                                                              aTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt                                         <  2:129967/140‑1 (MQ=255)
                                                                                              aTCAATCCGCGGGGATTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt                                         <  2:3951731/140‑1 (MQ=255)
                                                                                               tCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTg                                        >  2:2726657/1‑140 (MQ=255)
                                                                                                 taTCCGCGGGGTTTTTTTATTCAATCATCTGGGCCCTTCCATGTCCGAAAGCCCAATCCTCAGCCTGATTTCCGGCCAGCATGATACACACATCTTCTTTTCTTACGCCAAGGTCGGAATGAATCCGGCTTGCGATTGAg                                      <  2:3716952/139‑1 (MQ=255)
                                                                                                 aaTCCGCGGGGTTTTATTATTCAATGATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAATCTCGGAATGAATCCGGCTTGCGATTGAg                                      <  1:2118918/140‑1 (MQ=255)
                                                                                                 aaTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAg                                      <  1:1007748/140‑1 (MQ=255)
                                                                                                      gcgGGGTTTTATTATTCAATCCTCGGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCGTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTa                                 <  1:3405726/140‑1 (MQ=255)
                                                                                                      gcgGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTa                                 >  1:1378794/1‑140 (MQ=255)
                                                                                                      gcgGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTATTTTCTTAATCTAAGGTAGTAATTAATACTGCTTGCGATTGAggtgta                                 <  2:216012/140‑6 (MQ=255)
                                                                                                      gcgGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCGTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTa                                 <  1:218088/140‑1 (MQ=255)
                                                                                                       cgGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTaa                                >  1:1187964/1‑140 (MQ=255)
                                                                                                        ggggTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTaaa                               <  1:1397692/140‑1 (MQ=255)
                                                                                                        ggggTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTaaa                               <  1:3321318/140‑1 (MQ=255)
                                                                                                        ggggTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATATTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTaaa                               <  1:4000503/140‑1 (MQ=255)
                                                                                                        ggggATTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTaaa                               <  2:2143748/140‑1 (MQ=255)
                                                                                                           gTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt                                         <  2:902790/127‑1 (MQ=255)
                                                                                                             tttATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGct                          <  1:4260467/140‑1 (MQ=255)
                                                                                                             tttATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGct                          <  2:1691486/140‑1 (MQ=255)
                                                                                                             tttATTATTCAATCATCTGGGCCCTGCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGct                          <  2:1814940/140‑1 (MQ=255)
                                                                                                                  tattCCATCATCGGGGCCCTTCCAGTTCCGACAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTGTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCtttt                     <  2:2396503/140‑1 (MQ=255)
                                                                                                                  tattCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCAGCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCtttt                     <  2:381976/140‑1 (MQ=255)
                                                                                                                  tattCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCtttt                     <  2:1447682/140‑1 (MQ=255)
                                                                                                                  tattCAATCATCGGGGCCCTTCCAGTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTGTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCtttt                     <  2:3903199/140‑1 (MQ=255)
                                                                                                                  tattCAATCATCAGGGCCCGTCCAGTTCCGAAAGACCACTCCTCATCCTGAGTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCACGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCtttt                     <  2:865421/140‑1 (MQ=255)
                                                                                                                    ttCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCt                   >  2:3426654/1‑140 (MQ=255)
                                                                                                                      cAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTa                                 <  1:3699847/124‑1 (MQ=255)
                                                                                                                      cAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCAAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGt                 >  2:1169801/1‑140 (MQ=255)
                                                                                                                        atcatcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTc               <  2:1718323/140‑1 (MQ=255)
                                                                                                                        atcatcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTc               <  2:1612343/140‑1 (MQ=255)
                                                                                                                        atcatcTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTc               <  2:3225742/140‑1 (MQ=255)
                                                                                                                          catcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACCTCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  2:944883/140‑1 (MQ=255)
                                                                                                                          catcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  2:1986730/140‑1 (MQ=255)
                                                                                                                          catcTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTGTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  2:1045582/140‑1 (MQ=255)
                                                                                                                          catcGGGGCCGTTCCATGTCCGAAAGAACAATCCTCATCCTGATTTCCCGCCATCATGATAAACCCCACTTCTTTTCTGACGCCCAGCTCCGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAg             <  1:2487346/140‑1 (MQ=255)
                                                                                                                            gcTGGGCCCGTCCAGTGCCGACAGACCAATCCTCATCCTGAGTTCCGGCCAGCATGATAAACACCTCTTCTTTTCTTACGCCAAGCTCGGAATGAAGCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAgtt           <  2:2277484/139‑1 (MQ=255)
                                                                                                                                    cTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGa   <  1:811403/140‑1 (MQ=255)
                                                                                                                                     ttCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAc  >  1:4231108/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                           
TCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGCGGTAAAGTGCTCTTTTCTGTTCAGTTGTTCTTGAC  >  NZ_CP034943/3184628‑3184900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: