Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,201,057 C→G 6.1% L226V (CTG→GTG)  EO946_RS16570 → spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,201,0570CG 6.1% 705.2 / 6.2 249L226V (CTG→GTG) EO946_RS16570spore germination protein
Reads supporting (aligned to +/- strand):  ref base C (105/121);  new base G (17/2);  total (125/124)
Fisher's exact test for biased strand distribution p-value = 2.35e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

GAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTCATTGAAAGGT  >  NZ_CP034943/3200925‑3201195
                                                                                                                                    |                                                                                                                                          
gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTATTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATTAAGAAAAATAAATAAACATTAAGATATGCGGCTGTCGGCTTTCTGATTca                                                                                                                                     >  2:1222934/1‑139 (MQ=255)
gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTACATCTCGTTTTTAGGAAAGGAAGTGATTCTGTTTATTCCTGAACAAATGAATAAAAAGAAAAAAACAATCAGATATTCGGCTGTAGGCGTTATGAttac                                                                                                                                     >  1:57245/1‑138 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATGGCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACCTATGAAGAAAAAGAAATACAAATTCAGATATGGGGCTGTGGGGTTTCTGATTGCGa                                                                                                                                   >  2:1424482/1‑140 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATTAAGAAAAAGAAATAAACATTAAGATATTCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                                   >  2:2066608/1‑140 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTTTCGGCTTTATGATTACGa                                                                                                                                   >  2:3406298/1‑140 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                                   >  2:1989690/1‑140 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                                   <  2:3603214/140‑1 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                                   <  1:1386391/140‑1 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                                   >  2:537964/1‑140 (MQ=255)
  aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTGTACATCTCTTTTTTAGGAAAGGAAGTGATGCTGTTTCTGGCTGAACATATGTAGAAAAACAAATACACATTCAGATATGCGGCTGTCGGGTTTCTGCTTGCgg                                                                                                                                   >  1:1926119/1‑139 (MQ=255)
  aGGTCTTGGACCCATTGCTAACACTCTTACTGTCGTTTGGATCTGTTTTTTAGGTAAGGTCGTGATGCTGTTTGTTGCGGAACAGATGAAGCAAAAGAAATACAGATTCAGATATGCGGCTGTCGGCTGTGTGATTGCgg                                                                                                                                   >  2:1264263/1‑139 (MQ=255)
  aGGTCGTGGACCCATTGCTAACTCTCTTACTGTCGGTTCCATCTCTTTTTGAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATAGGCGGCTGTCGGCTTTCTGATTCCGa                                                                                                                                   <  2:4071047/140‑1 (MQ=255)
      cTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattat                                                                                                                               <  2:337603/140‑1 (MQ=255)
      cTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattat                                                                                                                               <  1:234351/140‑1 (MQ=255)
        tGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTATGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATAAAAAATAATATATGCGGCTGTCGGCTTTCTGATTGCGATTAttt                                                                                                                             >  1:2006279/1‑140 (MQ=255)
          gACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTta                                                                                                                           <  1:3393951/140‑1 (MQ=255)
           aCCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTtat                                                                                                                          <  2:1360676/140‑1 (MQ=255)
               aTTGCTAACTCTCTTACTGTCGTTTGCATCTGTTTTTTAGGAATTGAAGTGATGCTGTTTGTTACTGAACATATTAAGAAAAATAAATACAAAATCACATATGCGGCTGTAGGCTTTCTGATTCCGAATATTTTatatat                                                                                                                      >  1:3702920/1‑140 (MQ=255)
               aTTGCTAACTCTCTTACTGTCGTTTCCCACTATTTTTTTGGTATGGTAGTGATGCGTTTTCTTGCAAAACATATTAAAAAAAAAAAATAAACAATAACAAATTAGGCTGTCGCCTTTCTGAATGCCATTATTTTataaat                                                                                                                      >  1:1045634/1‑140 (MQ=255)
               aTTGCTAACTCTCTTACTGTCGTTTCCATCTGTTTTTTATGCATTGAAGTTATGCTGTTTGTTGCTGCACATATTAAGTAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTACGATTATTTTatatat                                                                                                                      >  1:3847749/1‑140 (MQ=255)
               aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTGCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                                                                                                      >  2:1896754/1‑140 (MQ=255)
               aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTGGGCTTTGTGATTGCGATTATTTTatatat                                                                                                                      >  2:366037/1‑140 (MQ=255)
               aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAACTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                                                                                                      >  2:2634407/1‑140 (MQ=255)
                ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                                                                                                     <  2:3304496/140‑1 (MQ=255)
                ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                                                                                                     <  2:3709500/140‑1 (MQ=255)
                ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                                                                                                     <  2:709173/140‑1 (MQ=255)
                agacagaCTCTCTTACTGTCGTGTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                                                                                                     <  2:2959620/134‑1 (MQ=255)
                      aCTCTCTTACTGTCGTTTGCCTCTCTTTTTTTTGAATGGAACTGATGCTGTTTCTTCCTGAACAGATGAAGAAAAAGAAAAAAACAATCAGATATGCGGCTGTGGGCTTTCTGGTTGCGATTATTTTATATATACTGaaa                                                                                                               >  1:279011/1‑138 (MQ=255)
                      aCTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACa                                                                                                               <  2:1963119/140‑1 (MQ=255)
                      aCTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACa                                                                                                               <  2:429550/140‑1 (MQ=255)
                       ctctctTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGTTGCTGTTTCTTCCGGAACATATTAAGAAAAAGAAATACAAAATCAGATATGCGGCTGTCGGCGTTCTGATTCCGAGTATTTTATATATACTGACat                                                                                                              >  1:3056239/1‑140 (MQ=255)
                         ctctTACTGTCGTTTACATCTCTTTTTTATTAATGGAAGTGATGCTGTTTCTTCCTGAACTTATGATGAATAATATTTAAACTTTCATATATTCTGCTTTCGTGTTTTTTATTTTTATTATTTTTTATATCCTTAGatat                                                                                                            <  2:825079/140‑1 (MQ=255)
                           ctTACTGTCGTTTCCATCTCTTTTTTAGGAATTGAAGTGATGCTGTTTGTTCCTGAACATATGAAGAAAAAGAAATACACATTAAGATATGCGGCTGTGGGCGTTGTGATTGCGATTATTTTATATATACAGACATATGt                                                                                                          >  1:2872299/1‑140 (MQ=255)
                                  tCGTTTCCATCTCTTTTTTAGGAATTGAAGTGATGCTGTTTCTTCCTGAACATAAGAAGAAAAAGAAATACACATTAAGATATGCGGCTGTCGGAGTTGTTATTCCCATTATTTTATATATCCTTAAATATGTAATAgtt                                                                                                   >  2:3454700/1‑140 (MQ=255)
                                  tCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATTAAGAAAAAGAAATACACATTCAGATATTCGGCTGTCGGGTTTCTGATTCCGATTATTTTATATATCCTTACAAGTGTGATGgtt                                                                                                   >  2:4160892/1‑140 (MQ=255)
                                  tCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTACGATTATTTTATATATACTGACATATGTCATCgtt                                                                                                   >  2:941788/1‑140 (MQ=255)
                                  tCGTTTCCATCTCTTTTTTAGGAAAGGAAGTGATGCTGTTTGTTACTGAACAAATCAACAAAAATAAATAAACATTCAGATATGCGGCTGTCGGCGTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCggt                                                                                                   >  2:2418040/1‑138 (MQ=255)
                                  tCGTTTCCATCGGTTTTTTAGGAATGGAAGTGATGCGGTTTCTTGCGGAACATATGAATAAAAAGAAATACAAATTCAGATATGCGGCTGTCGGCTTTGTGATTCCGATTATTTTATATATAATGACATATGTCATCgtt                                                                                                   >  2:1501231/1‑140 (MQ=255)
                                    gTTTCCATCTCTTTTTTATGAATGGAACTGATGCTGTTTCTTCCTGAACAAATGAAGAAAAATAAATACACAATCAGATATGCGGCTGTGGGGTTTCTGATTCCGATTATTTTATATATCCTGACATATTTCAACgttgt                                                                                                 >  2:1833236/1‑140 (MQ=255)
                                    gTTTCCAACTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTTATTCCCATTATTTTATATATCCTGACAAATTTCATAgtttt                                                                                                 >  2:4000225/1‑138 (MQ=255)
                                     tttCCATCTCTATTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgtt                                                                                                <  2:2301762/140‑1 (MQ=255)
                                     gttCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgtt                                                                                                <  2:1517361/139‑1 (MQ=255)
                                                tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                                     <  1:1014392/140‑1 (MQ=255)
                                                tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                                     <  1:3243275/140‑1 (MQ=255)
                                                tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                                     <  2:3702920/140‑1 (MQ=255)
                                                tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                                     <  2:1045634/140‑1 (MQ=255)
                                                tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                                     <  2:513182/140‑1 (MQ=255)
                                                         aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                                            >  1:4158677/1‑140 (MQ=255)
                                                         aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                                            <  1:3967635/140‑1 (MQ=255)
                                                         aTGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGa                                                                            >  1:4009141/1‑140 (MQ=255)
                                                                  aTGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAAc                                                                   >  2:208806/1‑140 (MQ=255)
                                                                      tGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGAGTCTTTTATATATCCTGAGATATGTCATCGTTGTTGGGGCGTTGACCGCTCCCGAGGTGAAATCGCTg                                                               >  1:4015411/1‑140 (MQ=255)
                                                                        tttCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGat                                                             >  2:2539108/1‑140 (MQ=255)
                                                                          tcttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGatat                                                           >  2:3021020/1‑140 (MQ=255)
                                                                          tcttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGatat                                                           >  1:2277775/1‑140 (MQ=255)
                                                                            ttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTCTATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATgg                                                         <  2:3646022/140‑1 (MQ=255)
                                                                            ttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATgg                                                         <  2:2917545/140‑1 (MQ=255)
                                                                            ttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATgg                                                         <  1:631626/140‑1 (MQ=255)
                                                                            ttcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATgg                                                         <  2:789023/140‑1 (MQ=255)
                                                                             tcCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGc                                                        <  2:3132849/140‑1 (MQ=255)
                                                                              ccTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                                       <  1:69668/140‑1 (MQ=255)
                                                                              ccTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                                       <  2:617250/140‑1 (MQ=255)
                                                                              ccTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                                       <  2:3084220/140‑1 (MQ=255)
                                                                              ccTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                                       <  1:3454700/140‑1 (MQ=255)
                                                                              catGAACATATGAAGAAAAATAAATACACATTCAGCTCTGCGGCTGTCGGCTTTCTGATTCCGATTATTGTCTATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                                       <  1:2476600/138‑1 (MQ=255)
                                                                               cTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCGGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCg                                                      >  2:1323865/1‑140 (MQ=255)
                                                                                  aaCATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTCTATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc                                                                                     <  1:1238086/106‑1 (MQ=255)
                                                                                  aaCATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACg                                                                  >  1:2011830/1‑125 (MQ=255)
                                                                                    cATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACtat                                                 <  1:1005468/140‑1 (MQ=255)
                                                                                    cATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACtat                                                 <  1:2634407/140‑1 (MQ=255)
                                                                                       atGAAGAAAAAGAAATTCACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTAGTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCGGAGCTGGCCGACTctatg                                              >  1:2063628/1‑139 (MQ=255)
                                                                                       atGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGa                                                     <  2:112036/133‑1 (MQ=255)
                                                                                       atGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtc                                              >  2:914107/1‑140 (MQ=255)
                                                                                       atGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGGTGGGGCTTTGACCGCTCCCGAGGTGACATCGCGGCTCTGGCCGACtctagc                                              >  2:2705909/1‑138 (MQ=255)
                                                                                       atGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGGTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATAtc                                              >  2:3240537/1‑140 (MQ=255)
                                                                                          aagaaaaagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGcc                                                       <  1:915159/128‑1 (MQ=255)
                                                                                             aaaaagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCtt                                        <  2:2872269/140‑1 (MQ=255)
                                                                                               aaagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGTTTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTGTc                                      >  1:2960722/1‑140 (MQ=255)
                                                                                               aaagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTc                                      >  1:4236707/1‑140 (MQ=255)
                                                                                               aaagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTc                                      <  2:3898141/140‑1 (MQ=255)
                                                                                               aaagaaaTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTc                                      <  2:201247/140‑1 (MQ=255)
                                                                                                     aTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCt                                <  1:2073863/140‑1 (MQ=255)
                                                                                                     aTACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCt                                <  1:1378247/140‑1 (MQ=255)
                                                                                                         acaTTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGa                            <  1:1167288/140‑1 (MQ=255)
                                                                                                           aTTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGc                          <  2:279011/140‑1 (MQ=255)
                                                                                                             tCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTc                        >  1:2594111/1‑140 (MQ=255)
                                                                                                              ctgATTTGCGGCTGTCGGCTTTGTGATTCCGATTATTTTATATATCGTGACATTTGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCTGTCCTTTGAGCTCa                       <  1:517459/138‑1 (MQ=255)
                                                                                                                   atGCGGCTGTCGGCTGTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGa                  <  2:400935/140‑1 (MQ=255)
                                                                                                                     gCGGCTGTCGGCTTTCTGATTCCGATTCTTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTCTATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACt                >  1:1540618/1‑140 (MQ=255)
                                                                                                                     gCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAACACGCTGATATGGCCGACTAGATCTCTCTTGCAGTCCTTTGAGCTCAAAGGACt                >  1:2797047/1‑140 (MQ=255)
                                                                                                                     gCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACt                >  1:1699121/1‑140 (MQ=255)
                                                                                                                     gCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACt                >  1:3654779/1‑140 (MQ=255)
                                                                                                                      cggctgtcggctgtCTTATTCCGATTATTTTATATATCCTGACATAGGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAATCGCTGATCTGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACtc               >  1:327463/1‑139 (MQ=255)
                                                                                                                         cTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTc            >  2:214318/1‑140 (MQ=255)
                                                                                                                         cTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATAGGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTAtgc            >  1:896798/1‑138 (MQ=255)
                                                                                                                           gTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTCAt          >  2:2586203/1‑140 (MQ=255)
                                                                                                                           gTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTCAt          >  1:3119516/1‑140 (MQ=255)
                                                                                                                              ggCTTTGTGTTTTCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGTGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATGTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTCATTGa       <  1:182766/140‑1 (MQ=255)
                                                                                                                               gCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTCATTGaa      <  2:172759/140‑1 (MQ=255)
                                                                                                                                   tCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTCATTGAAAGGt  >  1:2313806/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                          
GAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCCCGAGGTGAAAACGCTGATATGGCCGACTATATCTCTCTTTCAGTCCTTTGAGCTCAAAGGACTATTCATTGAAAGGT  >  NZ_CP034943/3200925‑3201195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: