Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,247,0650CT100.0% 674.0 / ‑6.6 196intergenic (‑11/+110)bdbD/cadAdisulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (119/74);  minor base A (1/1);  total (120/76)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

CTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGACG  >  NZ_CP034943/3246940‑3247199
                                                                                                                             |                                                                                                                                      
cTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTACGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaatt                                                                                                                          >  2:3138601/1‑140 (MQ=255)
agtCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTttaaaatt                                                                                                                          >  1:2730035/3‑140 (MQ=255)
        ccTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTaaaaaaac                                                                                                                  >  1:1323639/1‑139 (MQ=255)
        ccTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaag                                                                                                                  >  2:1663927/1‑140 (MQ=255)
          ttGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaa                                                                                                                >  2:13012/1‑140 (MQ=255)
           tggtCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               <  1:3745345/137‑1 (MQ=255)
           tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               >  1:3514835/1‑140 (MQ=255)
           tGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAaagaag                                                                                                               >  2:3312207/1‑140 (MQ=255)
             ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTATTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                             >  1:807355/1‑140 (MQ=255)
             ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTATAAAAAGAAGCg                                                                                                             >  1:3572521/1‑140 (MQ=255)
             ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                             <  2:1839801/140‑1 (MQ=255)
             ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                             >  1:4214714/1‑140 (MQ=255)
             ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                             >  1:3731765/1‑140 (MQ=255)
             ttCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGAACGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCg                                                                                                             >  2:2408535/1‑140 (MQ=255)
               ccGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                           <  1:38806/140‑1 (MQ=255)
               ccGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAAACACAACCGTAAGGATGACCGCGAATTTCGAAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGa                                                                                                           <  2:3248165/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCCCAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCGTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:497233/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:2904194/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:66232/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:3497258/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:902523/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:208001/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  2:307258/140‑1 (MQ=255)
                cGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTATTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGAt                                                                                                          <  1:496272/140‑1 (MQ=255)
                  ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  2:3213532/1‑140 (MQ=255)
                  ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  1:383606/1‑140 (MQ=255)
                  ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  1:848450/1‑140 (MQ=255)
                  ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  2:2929663/1‑140 (MQ=255)
                  ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  1:1975898/1‑140 (MQ=255)
                  ttttGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCg                                                                                                        >  1:2679283/1‑140 (MQ=255)
                       ttattaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGGTTTAAAATTAAAAAAAGAAGAGGATCGCAATa                                                                                                   >  2:2574644/1‑140 (MQ=255)
                         attaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGa                                                                                                 <  1:1032218/140‑1 (MQ=255)
                         attaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGa                                                                                                 <  1:2228179/140‑1 (MQ=255)
                         attaAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGa                                                                                                 <  1:4105078/140‑1 (MQ=255)
                           taAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                               <  2:2730035/140‑1 (MQ=255)
                           taAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                               <  2:4035518/140‑1 (MQ=255)
                           taAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGAcc                                                                                               <  1:3009784/140‑1 (MQ=255)
                            aaaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAATAAGAAGCGGATCGCAATAGAccc                                                                                              >  2:1120749/1‑140 (MQ=255)
                             aaGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCa                                                                                             <  2:4231102/140‑1 (MQ=255)
                                 tGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtc                                                                                         >  1:3297384/1‑140 (MQ=255)
                                 tGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtc                                                                                         >  1:1201442/1‑140 (MQ=255)
                                 tGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtc                                                                                         >  1:3612645/1‑140 (MQ=255)
                                  gACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCActtct                                                                                        <  2:3899855/140‑1 (MQ=255)
                                       tGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtt                                                                                   >  2:3010266/1‑140 (MQ=255)
                                       tGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTAtt                                                                                   >  2:1521005/1‑140 (MQ=255)
                                          ctgctAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTAATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTa                                                                                >  1:3315646/1‑140 (MQ=255)
                                                 tAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagac                                                                         <  2:2699218/140‑1 (MQ=255)
                                                 tAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagac                                                                         <  2:3731765/140‑1 (MQ=255)
                                                 tAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagac                                                                         <  2:1544198/140‑1 (MQ=255)
                                                 tAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAacaagac                                                                         <  2:807355/140‑1 (MQ=255)
                                                     acaacaACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGt                                                                     <  2:1813089/140‑1 (MQ=255)
                                                     acaacaACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGt                                                                     >  1:3564688/1‑140 (MQ=255)
                                                       aacaacCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGc                                                                   >  2:930680/1‑140 (MQ=255)
                                                       aacaacCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGc                                                                   >  2:67275/1‑140 (MQ=255)
                                                        acaaccaccaccGGACGGAGGACCGCGAATTTCGCAGAAGACTGCTGTTTCTGATTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCCCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                                  <  2:1168724/140‑1 (MQ=255)
                                                        acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGACGCCTGCTGTTTCTTTTTCACTCCGACACCTAATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGcc                                                                  <  2:1147301/140‑1 (MQ=255)
                                                        acaaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCCCTTCTTTATTTTAACAAGACAAGTggc                                                                  >  1:3989787/1‑138 (MQ=255)
                                                         caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCt                                                                 >  1:2017648/1‑140 (MQ=255)
                                                         caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCt                                                                 >  1:3084105/1‑140 (MQ=255)
                                                         caaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCGTATCGTTTTAAAATTAAACAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACACGACAAGTGCCt                                                                 >  2:4096824/1‑140 (MQ=255)
                                                          aaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCta                                                                >  1:913145/1‑140 (MQ=255)
                                                          aaccacaaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCta                                                                <  2:1886585/140‑1 (MQ=255)
                                                               caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                           >  1:1266724/1‑140 (MQ=255)
                                                               caaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGa                                                           >  2:3439240/1‑140 (MQ=255)
                                                                aaccGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGaa                                                          >  2:3688080/1‑140 (MQ=255)
                                                                       aGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAg                                                                                                  <  1:2159260/93‑1 (MQ=255)
                                                                       aGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAg                                                                                                  >  2:2159260/1‑93 (MQ=255)
                                                                       aGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCg                                                   >  2:1142131/1‑140 (MQ=255)
                                                                            gACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaa                                              >  1:1313095/1‑140 (MQ=255)
                                                                             acagcgAATTTCGCAGAAGACTGATGTTTATTTTTAACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGAGGATCGCAATAGACACACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1526165/4‑140 (MQ=255)
                                                                             aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCTGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:3910270/1‑140 (MQ=255)
                                                                             aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGa                                                                                                 >  1:3894590/1‑88 (MQ=255)
                                                                             aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1073240/1‑140 (MQ=255)
                                                                             aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:1364463/1‑140 (MQ=255)
                                                                             aCCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGaaaaaa                                             >  1:2566915/1‑140 (MQ=255)
                                                                              ccGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAAt                                            >  1:469698/1‑140 (MQ=255)
                                                                              ccGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAAt                                            >  1:3418108/1‑140 (MQ=255)
                                                                              ccGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAAt                                            >  2:466189/1‑140 (MQ=255)
                                                                                 cgAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGaa                                         <  1:1289682/140‑1 (MQ=255)
                                                                                        cGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATtctctc                                  >  1:3269557/1‑140 (MQ=255)
                                                                                        cGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATtctctc                                  >  1:2449074/1‑140 (MQ=255)
                                                                                           agaagaCTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGa                               <  1:1771298/140‑1 (MQ=255)
                                                                                                aCTGCTGTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAAtt                          >  1:4036658/1‑140 (MQ=255)
                                                                                                  tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                        >  1:1598310/1‑140 (MQ=255)
                                                                                                  tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                        >  2:601911/1‑140 (MQ=255)
                                                                                                  tgctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTa                        >  1:2861455/1‑140 (MQ=255)
                                                                                                   gctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTAt                       <  2:2566915/140‑1 (MQ=255)
                                                                                                   gctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTAt                       <  1:1663927/140‑1 (MQ=255)
                                                                                                    ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATc                      >  2:2811070/1‑140 (MQ=255)
                                                                                                    ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATc                      >  2:141408/1‑140 (MQ=255)
                                                                                                    ctgTTTCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATc                      >  2:2759452/1‑140 (MQ=255)
                                                                                                         tCTTTTTCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGttt                 >  1:3212981/1‑140 (MQ=255)
                                                                                                             tttCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga             <  2:3817367/140‑1 (MQ=255)
                                                                                                             tttCACTCCGACACATTATCTTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTACTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTga             <  2:4241762/140‑1 (MQ=255)
                                                                                                               tCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTgaga           <  1:3444541/140‑1 (MQ=255)
                                                                                                               tCACTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTgaga           <  1:4244194/140‑1 (MQ=255)
                                                                                                                 aCTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACg         >  2:2827375/1‑140 (MQ=255)
                                                                                                                 aCTCCGACACCTTATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACg         >  2:956411/1‑140 (MQ=255)
                                                                                                                      gACACCTTCTCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGa    <  2:550790/140‑1 (MQ=255)
                                                                                                                      gACACCTGATCGATTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGa    <  2:3900506/140‑1 (MQ=255)
                                                                                                                             |                                                                                                                                      
CTTCATTGCCTTGTTCCGTTTTGTTATTAAAGATGACAATGGCTGCTAGTAAGACAACAACCACAACCGTAAGGATGACCGCGAATTTCGCAGAAGACTGCTGTTTCTTTTTCACTCCGACACCTCATCGTTTTAAAATTAAAAAAAGAAGCGGATCGCAATAGACCCACTTCTTTATTTTAACAAGACAAGTGCCTATAAGAAAACCCCGAAAAAATGAATTCTCTCCGACAATTTATCCGTTTTTGAGACGCATGACG  >  NZ_CP034943/3246940‑3247199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: