Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,294,893 T→G 5.9% N293H (AAC→CAC)  eno ← phosphopyruvate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,294,8930TG 5.9% 728.1 / 4.2 290N293H (AAC→CAC) enophosphopyruvate hydratase
Reads supporting (aligned to +/- strand):  ref base T (133/129);  new base G (25/2);  total (159/131)
Fisher's exact test for biased strand distribution p-value = 1.97e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TGATCAGGATAGAGTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTC  >  NZ_CP034943/3294755‑3295031
                                                                                                                                          |                                                                                                                                          
  aTCAGGATAGAGTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTc                                                                                                                                         >  1:113312/1‑140 (MQ=255)
  aTCAGGATAGAGTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTGTTc                                                                                                                                         >  2:3906804/1‑140 (MQ=255)
  aTCAGGATAGAGTTGCCTAAGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGACAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTGTTc                                                                                                                                         >  1:572041/1‑140 (MQ=255)
     aGGATAGAGGTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTc                                                                                                                                      <  2:2653011/140‑1 (MQ=255)
       gATAGAGTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCaa                                                                                                                                    >  1:2224030/1‑140 (MQ=255)
       gATAGAGTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTAGTCaa                                                                                                                                    >  1:2236519/1‑140 (MQ=255)
           gagTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACACGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTcc                                                                                                                                <  2:1649895/140‑1 (MQ=255)
           gagTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTcc                                                                                                                                <  2:2761662/140‑1 (MQ=255)
           gagTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTcc                                                                                                                                <  1:3047364/140‑1 (MQ=255)
            agTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCa                                                                                                                               >  1:4027044/1‑140 (MQ=255)
            agTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCa                                                                                                                               <  1:2964683/140‑1 (MQ=255)
            agTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCa                                                                                                                               >  1:848963/1‑140 (MQ=255)
            agTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCa                                                                                                                               <  1:1337892/140‑1 (MQ=255)
            agTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGGCAAGTCCa                                                                                                                               >  2:443071/1‑140 (MQ=255)
            agTTGCCTACGCCGTTTGTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTGAAGTCCa                                                                                                                               >  1:1991930/1‑140 (MQ=255)
              ttGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATc                                                                                                                             <  1:1170800/140‑1 (MQ=255)
              ttGCCTACGCCGTTTTTGATACCTTCAGCAAGATTTTTCTTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTTCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCCGTCGTTTTCGTCAAGTCCATc                                                                                                                             <  1:626629/140‑1 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACCAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGGTTTCGTCAAGTCCATCTTc                                                                                                                          >  2:3064268/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTTAACTTTTTTGCCAATACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTACTCAAATcccaatta                                                                                                                          >  1:2990823/1‑134 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTGGTGAACTACATCtta                                                                                                                          >  1:1420297/1‑139 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGt                                                                                                                                  >  2:2323389/1‑132 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATcgtg                                                                                                                          >  2:1190317/1‑137 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCttg                                                                                                                          >  1:2330954/1‑139 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                          >  1:2159708/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                          >  2:1025236/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                          >  2:3739040/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                          >  2:1260508/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTc                                                                                                                          >  2:3004005/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGGCAAGTGCATCTTc                                                                                                                          >  2:664433/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTAGTGAAGTCCATcata                                                                                                                          >  1:446445/1‑137 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTGTGCGTCCAGTCCATCTTc                                                                                                                          >  1:737333/1‑140 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGACAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGGCGTGTTCGGAAAGTCCATcgtg                                                                                                                          >  1:1779298/1‑137 (MQ=255)
                 ccTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGGTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTGTTCGTCAAGTCCaaattc                                                                                                                          >  1:1541786/1‑135 (MQ=255)
                 ccTACGCCCTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTAGTCAATTACATGTTc                                                                                                                          >  2:3657620/1‑140 (MQ=255)
                   gccGCCGTTTTTGAGACCTTCAGCAAGTTTTTTCGTGTGTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGa                                                                                                                        <  2:2902907/138‑1 (MQ=255)
                    aCGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCGGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGAt                                                                                                                       <  2:1084984/140‑1 (MQ=255)
                      gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTTCCAAGACGCTCAGTAAGAAGCTTTTGGCCTTCCCAGTCGTTTTAGTAAAGTCCAAAATCGATag                                                                                                                     >  1:1193073/1‑140 (MQ=255)
                      gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATGTTCGATag                                                                                                                     >  2:2059497/1‑140 (MQ=255)
                      gCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTGTTCGTCAAGTACATCTTCGATag                                                                                                                     >  1:3527089/1‑140 (MQ=255)
                              tGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGAtt                                                                                                             <  1:3999214/140‑1 (MQ=255)
                                  aCCGTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGt                                                                                                         <  2:1930346/140‑1 (MQ=255)
                                    cTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTAt                                                                                                       >  2:2282420/1‑140 (MQ=255)
                                    cTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTAAAGTACATATTCGATAGAGATGATTGGGTAt                                                                                                       >  2:3495944/1‑140 (MQ=255)
                                       cAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTa                                                                                                    <  2:693956/140‑1 (MQ=255)
                                       cAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTa                                                                                                    <  2:1211837/140‑1 (MQ=255)
                                         gCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGa                                                                                                  <  2:2441825/140‑1 (MQ=255)
                                         gCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGa                                                                                                  <  2:1106652/140‑1 (MQ=255)
                                               tttttCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCAt                                                                                            <  2:2184447/140‑1 (MQ=255)
                                               tttttCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCAt                                                                                            <  1:2586554/140‑1 (MQ=255)
                                                         ttGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTGTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTAc                                                                                  <  2:3620141/140‑1 (MQ=255)
                                                               cGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCa                                                                            <  2:1973249/140‑1 (MQ=255)
                                                                    tgttcatcaAGAACAATCTGAACTTTTTTGCCAAGACTCTCAGTAAGAAGCTTGTGGCCTTCCGAGTAGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCAt                                                                       <  1:2794087/137‑1 (MQ=255)
                                                                    agGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCGTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCAt                                                                       <  1:3209054/140‑1 (MQ=255)
                                                                      gTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGGATTTAGAAACCATTTCGTCGTACCAGTCAACCAttt                                                                     >  1:895267/1‑140 (MQ=255)
                                                                      gTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATATTCGATATAGATGTATGGGTATTTATAAAACATTTGTTCGTAACAGTCAACCAatt                                                                     >  1:678660/1‑140 (MQ=255)
                                                                         atcaCCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATATAGATGATTGGGTATTTAGAAACCAATTGTTCGTACCAGTCAAACATTTaag                                                                  >  1:3028577/1‑140 (MQ=255)
                                                                         atcaCCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCAACGTCGATAGAGATGATTTGGGATTTAGAAACCATTTCTTCGTACCAGGCAACCATTTcag                                                                  >  1:3632228/1‑140 (MQ=255)
                                                                         atcaCCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTAAAGTCAAACTGAGATAGAGATGATTGGGTATTTAGAAACCATTTCTTAGTAACAGTCAACCAATTcag                                                                  >  1:507729/1‑140 (MQ=255)
                                                                                acaaGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGGATTTAGAAACCCTTTCGTCGTACCAGTCAACCATTTCAGCAGATGt                                                           >  2:1039915/1‑140 (MQ=255)
                                                                                   aGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGTGATGATTGGGTAATTAGAAACCATTTCGTCGTACCAGTCAACCATTTCAGCAGATGtttt                                                        >  2:3363172/1‑140 (MQ=255)
                                                                                       taaCTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTcaca                                                    <  2:598247/139‑1 (MQ=255)
                                                                                       gAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTcaca                                                    <  1:1655805/140‑1 (MQ=255)
                                                                                        aaCTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACaa                                                   >  2:952962/1‑140 (MQ=255)
                                                                                           ttttttGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGc                                                <  2:4027044/140‑1 (MQ=255)
                                                                                             ttttGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTGGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTAAGCAGATGTTTTCACAACGCCt                                              >  2:2805669/1‑140 (MQ=255)
                                                                                             ttttGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCt                                              >  2:1882604/1‑140 (MQ=255)
                                                                                                 gCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGc                                          <  1:2177228/140‑1 (MQ=255)
                                                                                                 gCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGc                                          <  1:1685406/140‑1 (MQ=255)
                                                                                                   cAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCca                                        <  1:316549/140‑1 (MQ=255)
                                                                                                   cAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCca                                        <  1:3411554/140‑1 (MQ=255)
                                                                                                   cAAGACGCTCAGTAAGAAGCTTGTGGCCGTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCca                                        <  2:3225273/140‑1 (MQ=255)
                                                                                                         gCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCcagacaga                                  <  2:1752328/140‑1 (MQ=255)
                                                                                                          cTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGAt                                 <  2:4186198/140‑1 (MQ=255)
                                                                                                          cTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGAt                                 <  2:1549757/140‑1 (MQ=255)
                                                                                                          cTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGAt                                 <  1:2822081/140‑1 (MQ=255)
                                                                                                                     gCTTGTGGCCTTCCCAGTCGTGTTCGTCAAGTCCATCGTCGATAGAGATTATTGGGTATTTAGAAACCATTTGTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAAAGCCGTCGCCACACACATGGTATTTTccc                      >  1:1917065/1‑139 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTTTTCGTGAAGTCCATATTCGCTAGAGATGATTGGGTATTTAGAAACAATTTCTTCGTACCAGTCAACCATTTAAGCAGATGTTTTCAAAACGCCATCGGCACACAGATGGTATTTGCCGgctt                  >  1:3697425/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGCTAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGtctt                  >  2:831478/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTAACAGTCAACCATTTCAGCAGATGTTTTCAAAACGCCTTAGCCAGACAGATGGTATTTGCCGtctt                  >  2:2973371/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAAACAATTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCGTCGCCAGACACATGGTATTTGCCGtctt                  >  1:4136128/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGGATTTAGAAACCATTTGTTCGTACCAGTCAACCATTTCATCCGATTTTTTGACAACCCCTTCGCCAGACAGATGGTATTTTCCGtgtt                  >  1:1095215/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTTTTCGTCAAGTACAACTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTAGTACCAGTCAACCATTTCAGCAGGTGTTTTAACAACGCCATCGCCAGACACATGGGAGTTGGCggcat                  >  2:2377430/1‑136 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTGTTGGTCAAGTCCATCTTCGATAGATATTTTTGGGTATTTTTAAACCATTTCTTCGTTCCAGTCAACCAATTAAGCAGATTTTTTAACAACGCCTTCGCCCGCCAGATGGTATTTTCCGtctt                  >  1:3773070/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTGTTCGTCAAGTCCATCTTCGATAGAGCTGATTGGGGATTTAGAAACCATTTCGTCGTACCACGCAACCAGTTCAGCACATGTTTTCACAACCCCGTCGCCAGACAGATGGTCTTTGCCGtgtt                  >  1:3479907/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTGTTCGTCAAGTCCAACTTCGATAGAGATGTTTGGGTATTTATAAACCATTTGTTCGTACCAGTCAACCAATTAAGCAGATGTTTTCTCAAAGCCATCGCCAGACAGATGGTATTTGCCGtctt                  >  1:1000833/1‑140 (MQ=255)
                                                                                                                         gtgGCCTTCCCAGTCGTGTTCGGCAAGTCCCTCTTCGATAGAGAAGAGTGGGTAGTTAGCAACCCTGTCTTCGTACCAGTCAACCATTTCAGCAGATGGTTTCAAAACGCCTTCGCCAGACCGATGGTCTTTGCCGtctt                  >  2:1991824/1‑140 (MQ=255)
                                                                                                                            gCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTcttctt               <  2:1497951/140‑1 (MQ=255)
                                                                                                                            gCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTcttctt               <  2:790267/140‑1 (MQ=255)
                                                                                                                              cTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTGTTCGTACCAGTCAACCATTTCATAAGATGTTTTCACAAAGCCTTCGCCAGACAGATGGTATTTTCCGTCTTCTttg             >  2:1378168/1‑140 (MQ=255)
                                                                                                                              cTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTttg             <  2:527905/140‑1 (MQ=255)
                                                                                                                              cTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGGATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTGTttg             >  2:3107457/1‑140 (MQ=255)
                                                                                                                              cTTCCCAGTCGTGTTAGTCAAGTCCATCTTCGATAGAGATTAGTGGGTATTTTGTAACCATTTATTCGTACCAGTCCACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGGATTTTCCGTCGTCTttg             >  2:1807577/1‑140 (MQ=255)
                                                                                                                                tCCCAGTCGTTTTCGTCAAGTCCCTCTTGGATAGAGATTATTTGGTATTTAGAAACCAATTATTCGTACCAGTCAACCATTTAAGCACATGTTTTGACAAAACCATAGCCACACACAAGTTATTTTCCGTATtgtttgtt           >  1:1554138/1‑140 (MQ=255)
                                                                                                                                tCCCAGTCGTTTTCGTCAAGTCCATCTTGGATAGAGATGATTGGGGATTTAGAAACCAATTGTTCGTACCAGGCAACCATTTAAGCAGATGTTTTAACAACGCCTTCGCCAGACAGATTGTATTTTCCGTCTtgtttgtt           >  1:2677601/1‑140 (MQ=255)
                                                                                                                                tCCCAGTCGTTTTCGTCAAGTCCATCTTCGCTAGAGATGATTGGGGTTTTAGAAACCATTTGTTCGTACCAGTCAACCCTTTAAGCAGATGTTTTAACAACGCCGTCGCCAGACAGATGGTATTTGCCGTCTTCGgtgtt           >  1:3284629/1‑140 (MQ=255)
                                                                                                                                   cAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTa        <  2:2235497/140‑1 (MQ=255)
                                                                                                                                   cAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTa        <  1:124180/140‑1 (MQ=255)
                                                                                                                                    aGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAg       <  1:3938413/140‑1 (MQ=255)
                                                                                                                                       cGTTTTCGTAAAGTCCAACTTCGATACAGATGAATGGGTATTTATAAAACATTTGGTCGTACCAGTCAACCATTTCAGCCGATGTTTTCACAACGCCGTCGCCAGAAACATGGTATTTGCAGTCTTCTTTGTTGTAGAAc    >  1:1039452/1‑140 (MQ=255)
                                                                                                                                         ttttCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTc  <  2:1156784/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TGATCAGGATAGAGTTGCCTACGCCGTTTTTGATACCTTCAGCAAGTTTTTTCGTGTTTGTAACGAAGAGGTCATCACCAACAAGCTGAACTTTTTTGCCAAGACGCTCAGTAAGAAGCTTGTGGCCTTCCCAGTCGTTTTCGTCAAGTCCATCTTCGATAGAGATGATTGGGTATTTAGAAACCATTTCTTCGTACCAGTCAACCATTTCAGCAGATGTTTTCACAACGCCTTCGCCAGACAGATGGTATTTGCCGTCTTCTTTGTTGTAGAACTC  >  NZ_CP034943/3294755‑3295031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: