Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,637,344 T→A 14.7% Y44N (TAC→AAC)  EO946_RS18740 → DUF2529 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,637,3440TA14.7% 832.5 / 113.8 296Y44N (TAC→AAC) EO946_RS18740DUF2529 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (142/109);  new base A (26/18);  total (168/128)
Fisher's exact test for biased strand distribution p-value = 8.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

GGATTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCGG  >  NZ_CP034943/3637205‑3637483
                                                                                                                                           |                                                                                                                                           
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCCTTCCATTTATTtata                                                                                                                                            >  2:3487579/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:3865181/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  1:2569306/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:2538692/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  2:1414423/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  1:3193114/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:332933/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  2:2289826/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  1:1908559/1‑140 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:1135958/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:1757333/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:55436/140‑1 (MQ=255)
 gaTTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGATGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  1:3993995/140‑1 (MQ=255)
   ttCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACg                                                                                                                                          <  2:2910955/140‑1 (MQ=255)
   ttCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACg                                                                                                                                          <  2:3759700/140‑1 (MQ=255)
    tCACTTATTTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACgg                                                                                                                                         <  2:3426015/140‑1 (MQ=255)
    tCACTTATGTTGAAACTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCAGTCCATTTATTTATACgg                                                                                                                                         <  2:2318335/140‑1 (MQ=255)
    tCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACgg                                                                                                                                         <  2:333369/140‑1 (MQ=255)
      aCTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTCTTTATACGGAg                                                                                                                                       >  2:1828887/1‑140 (MQ=255)
      aCTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAg                                                                                                                                       >  2:1697290/1‑140 (MQ=255)
           tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAAt                                                                                                                                  >  2:2040102/1‑140 (MQ=255)
           tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                                  >  1:3521870/1‑140 (MQ=255)
           tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGGGAACAGCGGGCATTCCATTTCTTTATACGGAGCTAAt                                                                                                                                  >  2:1360701/1‑140 (MQ=255)
            gTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATg                                                                                                                                 >  2:2135197/1‑140 (MQ=255)
             ttGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGa                                                                                                                                >  2:3872358/1‑140 (MQ=255)
                acaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTACTGAGCt                                                                                                                             >  2:1733872/3‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCt                                                                                                                             >  2:1576411/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCt                                                                                                                             >  2:900730/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCt                                                                                                                             >  2:2699034/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCt                                                                                                                             >  2:3257022/1‑140 (MQ=255)
                aaaaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCt                                                                                                                             >  1:1073041/1‑140 (MQ=255)
                aaaaTATTTACGACGAAGTTAACAGGAATTTTTTCCCGCATTCAGGATAAGGAATCTTACGCGATTGAAGATGGGAAGCGGATGATTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATAAGGAGCTAATGAGCt                                                                                                                             >  2:130419/1‑140 (MQ=255)
                      tttACGACGCAGTTAACTGGGAATGTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGGGGGCATTCCATTTTTTTATACGGAGCTAATGAGCTTCAggg                                                                                                                       <  2:2453584/140‑1 (MQ=255)
                      tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAggg                                                                                                                       <  1:4029238/140‑1 (MQ=255)
                      tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAggg                                                                                                                       <  1:1454202/140‑1 (MQ=255)
                                    aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                         <  1:278607/140‑1 (MQ=255)
                                    aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                         <  1:2107447/140‑1 (MQ=255)
                                    aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                         <  1:4216435/140‑1 (MQ=255)
                                       aGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCa                                                                                                      >  2:1245737/1‑140 (MQ=255)
                                          tATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCg                                                                                                   >  1:81751/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATCAGGAATCTGACGCGATTGAAGATGGGGCGCGGGTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTGATTTAAACGGAGCTAATGAGCTTCAGGGCGTGTTTTATGAGGCCACCGaa                                                                                                 >  1:2877035/1‑140 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            >  1:1793961/1‑97 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTtata                                                                                                                                            <  2:1793961/97‑1 (MQ=255)
                                            ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 <  1:1397652/140‑1 (MQ=255)
                                                 tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagcag                                                                                            <  2:2597960/140‑1 (MQ=255)
                                                   ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                          <  1:3575797/140‑1 (MQ=255)
                                                        ttCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAAccc                                                                                     <  1:3784452/140‑1 (MQ=255)
                                                               tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                              >  1:708998/1‑140 (MQ=255)
                                                                aaGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATc                                                                             >  1:993864/1‑140 (MQ=255)
                                                                  ggAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTg                                                                           >  2:692876/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCCGCGAACCCTTCCCATCTGTCaaa                                                                      >  2:1106412/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCcaa                                                                      >  2:3153349/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  2:65075/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  1:1602992/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  2:3061205/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  1:3834662/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                      >  2:3798791/1‑140 (MQ=255)
                                                                       cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATGTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTGAGGAGGCCACCGAAAGCTGCGACCCCTTCGCAGCTGTCaaa                                                                      >  2:2219844/1‑140 (MQ=255)
                                                                           cgcgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCt                                                                  <  2:3024148/140‑1 (MQ=255)
                                                                           cgcgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCAGTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCt                                                                  <  2:3925755/140‑1 (MQ=255)
                                                                               aTTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTcc                                                              >  1:1165006/1‑140 (MQ=255)
                                                                               aTTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTcc                                                              >  1:3439130/1‑140 (MQ=255)
                                                                               aTTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGCTGATCAGCGGGCATTCCATTTATTTATACGTAGCTAATGAGCTTCAGGGCGTCTTTTATGAGTCCACCGACAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTcc                                                              >  1:2728896/1‑140 (MQ=255)
                                                                                        ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGa                                                           <  1:2165954/134‑1 (MQ=255)
                                                                                        ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGa                                                           >  2:2165954/1‑134 (MQ=255)
                                                                                         gggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCt                                                    >  1:1285253/1‑140 (MQ=255)
                                                                                         gggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCt                                                    <  2:4054635/140‑1 (MQ=255)
                                                                                          ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGACAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGACCGCGCTg                                                   >  1:2240519/1‑140 (MQ=255)
                                                                                          ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTg                                                   >  1:2992142/1‑140 (MQ=255)
                                                                                             gcgGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                                 >  1:736670/1‑91 (MQ=255)
                                                                                             gcgGCTGCTTGCTCAAGCGGTGATCAGCGGGCAGTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAgg                                                <  2:2365648/140‑1 (MQ=255)
                                                                                                gctgctTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAggg                                                                                                                       <  2:282930/66‑1 (MQ=255)
                                                                                                      tgctCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAg                                       >  2:40963/1‑140 (MQ=255)
                                                                                                         tCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGaaa                                    >  2:3580681/1‑140 (MQ=255)
                                                                                                               ggTTATCAGCGGGATTTCCATTTATTTATACGTCGCTAATGAGCTTCAGGGCGTATTTTATGAGGCCACCTAAAGCAGCGAACCCTTCCCATCTTAAAAAGCCTTTCCACAAAGCGCTGAGGAAGTCACACAAAGCGACa                              <  1:2389695/140‑1 (MQ=255)
                                                                                                               ggTGAACAGCCGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGCCCACCGAAACCAGCCAACCCTTCCCCTCTTTCAAAGCATTTCCACAACGCGCTGAGGAAGTGACAGAACGCGACa                              <  1:4124829/140‑1 (MQ=255)
                                                                                                                gTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAg                             >  2:4149478/1‑140 (MQ=255)
                                                                                                                  gATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGAGACAggg                           >  2:561175/1‑140 (MQ=255)
                                                                                                                  gATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAggg                           >  2:766003/1‑140 (MQ=255)
                                                                                                                   aTCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                              <  2:1078835/136‑1 (MQ=255)
                                                                                                                       gggggCATTCAATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAACGCGCTGTGGAAGTGACAGAAAGCGACAGGGTGCTg                      <  1:1562184/138‑1 (MQ=255)
                                                                                                                       gggggAATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCAACCTAAAGCAGCGAACCTTTCCCATCTGTCAAAGCCTTCCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTg                      <  2:2740077/138‑1 (MQ=255)
                                                                                                                        cGGGCATTCCATTTATTTATACTGAGCTACTGAGCTTCAGGGCGTCTTTTATGTGGCCACCGAAAGCAGCGAACCCTTCCCATCTGACAAAGAATTTCAAGAAAGCGCTGAGTAAGTGACAGAAACAGACAGGCTGCGGa                     >  1:1085292/1‑140 (MQ=255)
                                                                                                                        cGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGa                     >  2:3179951/1‑140 (MQ=255)
                                                                                                                        cGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGa                     >  2:876283/1‑140 (MQ=255)
                                                                                                                        cGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGa                     >  2:1877454/1‑140 (MQ=255)
                                                                                                                        cGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGa                     >  2:531099/1‑140 (MQ=255)
                                                                                                                        cGGGCATTCCATGTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGTGGAAGTGACAGAAAGCGACAGGGTGCTGa                     >  1:522939/1‑140 (MQ=255)
                                                                                                                           gCATTCCATTTATTTATACGGAGCTAATTAGCTTCAGGGCGTCTTTTATGAGGCCACCTAAAGCAGCGAACCCTTCACATCAGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGAGACAGGGTGCTGATGt                  >  2:734051/1‑140 (MQ=255)
                                                                                                                           gCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCCAAGCCTTTCCAGAAAGCGCTGAGGACGTGACAGAAAGCGACAGGGTGCTGATGt                  >  2:3949828/1‑140 (MQ=255)
                                                                                                                           gCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGt                  >  2:4013528/1‑140 (MQ=255)
                                                                                                                           gCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGt                  >  2:1264177/1‑140 (MQ=255)
                                                                                                                            cATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCATAAAGCGCTGAGGAAGTGACAGAACGCGACAGGGTGCTGATGtt                 <  1:133329/140‑1 (MQ=255)
                                                                                                                               tCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGACGTGACAGAAAGCGACAGGGTGCTGAGGTTTTg              >  2:227035/1‑140 (MQ=255)
                                                                                                                                   tttatttaTAGGGAGATATTGAGATTCAGGGGGTATTTTATGCGGCCACCTAAAGCAGCGAACCTTTCCCATCTGTAAAAGCTTTTCCAGAACGCGCTGTGGAAGTGACACAAAGCGACAGGGTGCTGATGTTTTGCTCa          <  1:2289826/140‑1 (MQ=255)
                                                                                                                                   tttatttaTACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGCGGCCACCGAAAGCAGCGAACCCTTCCCATCTGACAAAGCCTTCCCCCAAAGCGCTGCGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCa          <  1:1850547/140‑1 (MQ=255)
                                                                                                                                        ttatACGGAGCTAATGAGCTTCCGGGCGTCTTTTAGGCGGCCACCGAAAGCAGCGAACCCTTCCAATCTGTCAAAGCCTTTCCAGAACGCGCTGAGGAATTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAc     <  1:764612/140‑1 (MQ=255)
                                                                                                                                         tataCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAcc    >  2:1497585/1‑140 (MQ=255)
                                                                                                                                           taCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCgg  >  1:3382958/1‑140 (MQ=255)
                                                                                                                                           taCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCgg  >  2:3689527/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GGATTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGACCGG  >  NZ_CP034943/3637205‑3637483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: