Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,440,3670TG55.0% 2.0 / 14.0 22W26G (TGG→GGG) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/4);  new base G (11/0);  total (18/4)
Fisher's exact test for biased strand distribution p-value = 2.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB122/3440237‑3440497
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gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCGGGCGGCGCCCCGGCCGCCgggggg                                                                                                                               >  6:6135/1‑134 (MQ=255)
                   cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCCGGCAGCCTCGGGGTGGCGGGCCCACCCCta                                                                                                              >  1:92584/1‑134 (MQ=255)
                    aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCCCGCCgat                                                                                                             >  2:59441/1‑134 (MQ=255)
                        cccTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCCGTGGCGGCGCCCGCGCGGGCGGGGGGTGGCGGGCCCACGCCTCTAATgcg                                                                                                       >  7:95979/1‑136 (MQ=255)
                                       cgcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCACGCCGATAAGGCGCCGCGCGCGGCTCa                                                                                         >  1:264429/1‑135 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCCCGGGGGGCGGCGCGGCCGCCGGGGGGTGGGCGGGCCCCCCCGCTAAAGCGCCGCTCGCTGATCAg                                                                                        >  2:327505/1‑135 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGGCCGGGCCGCCGGGGGGGGGGCGGCCCCCCCCCATAACGCGCCGCACGCGGATAAgg                                                                                       >  6:40642/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGAGCCCGGGGGTGGCCGGCCCACGGCGATAAGTCGCCGCGCGCTGCTCAgggc                                                                                     >  8:324138/1‑134 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCGGGGCGGTGGCAGGGCCCCCCCCATAAAGCGCCCCTCGGGGATCAGGcc                                                                                     >  3:301862/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCACGCCGATAAGGCGCCGCGCGCGGCTCAGGcc                                                                                     >  5:154162/1‑136 (MQ=255)
                                                     cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCCCCCCCGATAAGGCGCAGCTCGCGGCTCAGGCCGCTGACTGCgg                                                                          >  7:172262/1‑136 (MQ=255)
                                                     cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCGGGCCGCCCGGGGGTGGCCGGCCAACGCCCAAAAAGCGCCCCTCGCGGCTCAGGCCGCTGACttag                                                                           >  2:23512/1‑133 (MQ=255)
                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTTCTGATGGCCCCCACCGCGGGCGGCGCGCGGGCCGCCTGGGTGTGGGCGGCCAACCCCACTAACGCGCCGCTCGGTGCTGAGGCCGGGGGCTTGGGCGAGCAgg                                                                  >  4:299170/1‑135 (MQ=255)
                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCCGGCCAACCCCTATAAGGCGCAGCTCGCGGATCAGGCCGATGACTTGGGCACGCAgg                                                                  >  7:140461/1‑135 (MQ=255)
                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCCCCGGGGTGTGGGCGGCCCACGCCGATAAGGCGCAGCGCGCGGAGCAGGCCGCTGCCTGCGGCAAGCAGt                                                                  >  4:168894/1‑136 (MQ=255)
                                                                  ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCCCGCCGAGAAGGCGCCGCGCGCTGATCCGGCCGCTGGCGTCGGCAAGCAGGTGGCg                                                             >  7:77227/1‑136 (MQ=255)
                                                                          gCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGCGGGGCCCGGGCGGCCGGGGGGTGGGGGGGCCACGCCCATAATGCGGCGCGCGCTGAGCAGGCCGCGGCCGTAGGCCAGCAGGTGGGGGGGGAggg                                                     >  4:278881/1‑135 (MQ=255)
                                                                                gggCCGTGGCGCTGCTGCGGCCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTTGCAGGCCATCGCCTATAAGTCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCTGAGAAGTATcgcgc                                               <  8:5629/134‑1 (MQ=255)
                                                                                                  gggCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGga                             <  7:299929/135‑1 (MQ=255)
                                                                                                     ccgccCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                          <  4:124505/136‑1 (MQ=255)
                                                                                                                ggcggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcag               <  4:32304/136‑1 (MQ=255)
                                                                                                                   ggcgCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGcagcagcag            >  3:191040/1‑136 (MQ=255)
                                                                                                                              aGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa  >  2:295548/1‑135 (MQ=255)
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GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA  >  ACB122/3440237‑3440497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑