Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,880,3500AC53.3% 0.7 / 18.8 30G100G (GGT→GGG) CJ019_04574hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (7/7);  new base C (10/6);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCACCCATCCCGTTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGT  >  ACB122/4880220‑4880481
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ccccGGCATCTGCCCGCAGCCCCCCCTCCTCGATCGCCCTGTTCGCACCCAGCTCGTCGAGATCCGCCGTATCGCCGCTGCTCCCCCCGGCCCCCCGCCCCGTGCCGTCCGGCTCACGCAGCAGCCCCCCCCCCgc                                                                                                                                 >  5:260145/1‑135 (MQ=255)
ccccGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCgg                                                                                                                                 >  7:200862/1‑136 (MQ=255)
        tCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGCCGTATCGACGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGCTGACGCAGCCGCACCCCACCCGGCGCCGGcc                                                                                                                         >  7:326226/1‑135 (MQ=255)
               gCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                  <  5:149461/136‑1 (MQ=255)
               gCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCAGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                  <  7:25076/136‑1 (MQ=255)
                     ccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCt                                                                                                             <  2:48461/135‑1 (MQ=255)
                                     aCTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGCCGCACCCCCAAccccgtcca                                                                                             >  2:245879/1‑129 (MQ=255)
                                            gCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCCGCGCCGGCACCACCGGCCGCGCCCCCCTCCCGGTCAAC‑GCGGCa                                                                                     >  8:318624/1‑135 (MQ=255)
                                               cacTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCACCCATCCCGTTCAAC‑GCGGCaaaaa                                                                                 <  5:157749/136‑1 (MQ=255)
                                                      cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCCCCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCCCCCGCCGCGCCCCCCACCCGGTCAAC‑CCGGCCAAAAAAgcccg                                                                          >  3:236984/1‑134 (MQ=255)
                                                      cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCCCCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCCGCGCCGGCACCACCCGCCGCTCACCCCGCCCGTTCAAC‑CCGGCAAAAAAAgccgg                                                                          >  7:74904/1‑136 (MQ=255)
                                                      cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGCGCACCCATCCCCGTCAAC‑GCGGCAAAAAAAgccgg                                                                          >  6:166005/1‑136 (MQ=255)
                                                      cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCCGCGCCGCCCCCCCCCGCCGCCCACCCCACCCCTTCCAC‑CCGGCAAAAAAAaccg                                                                           >  1:218961/1‑135 (MQ=255)
                                                               ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGCCGCTCCCCCATCCCGGTCAAC‑GCGGCAAAAAAAGCCGGCCGcggctg                                                                 >  7:235468/1‑136 (MQ=255)
                                                               ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGCGCCCCCATCCCGGTCAAA‑GCGGCCAAAAAAGCCCGCCGctgctg                                                                 >  4:177990/1‑136 (MQ=255)
                                                               ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCCGCGCCGGCCCCCCCCGCCGCCCACCCCACCCGGTCAAC‑GCGGCAAAAAAAGCCCGCCCcggctg                                                                 >  6:85301/1‑136 (MQ=255)
                                                               ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGAGCACGAATCAGCACCCCACCCGGCGCCGGCACCCCCGGCCGCGCCCCCCTCCCGTTCAAC‑GCGGCAAAAAAAGccgcccgcatcag                                                                 >  5:117440/1‑131 (MQ=255)
                                                                          ccgcTGACCCCCCCGCCCCCCAGCACCTTCCCGGCCTGACCACGAAGCCCCCCCCCCCCCGGCGCCGGCACCCCCGGCCGCTCACCCAGCCCGTTCAAC‑GCGCCAAAAAACCCCGGCCGCTGCTGCGCATcc                                                          <  1:87963/132‑1 (MQ=255)
                                                                                   cccACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCACCCATCCCGTTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAgc                                             <  4:53472/136‑1 (MQ=255)
                                                                                   cccACGGCACCCACCACCTTGCCGTCCTCATCACCAACCCCCACCCCACCCGGCGCCGGCACCACCGCCCGCGCACCCAACCCGTACAAC‑GCGCCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAgc                                             <  5:85301/136‑1 (MQ=255)
                                                                                         ccACCCCGCCCCTGGCCGGCCTGAGCACGACTCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGCGCCCCCATCCCGTGCAAC‑GCGGCAACAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAg                                        <  1:62802/134‑1 (MQ=255)
                                                                                             ccAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGCTCACCCATCCCGTTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                   <  6:35628/136‑1 (MQ=255)
                                                                                             ccAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCACCCATCCCGTTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                   <  5:157472/136‑1 (MQ=255)
                                                                                              cAGCACGTTGCCGGCCTTACCCCTAATCCGCACCCCCCCCGGCGCGGGCACCCCGGGCCGCTCACCCATCCCG‑TCAACGGCGGCAAAAAACCCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                   <  2:218961/135‑1 (MQ=255)
                                                                                               aGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCACCCATCCCGGTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCCTCCCGCGCCCGCCAGCGCCCGCCCTTg                                  >  1:16293/1‑135 (MQ=255)
                                                                                                  aCCTTGCCGTCCTGATCACGAATCAGCACCCCACCCCGCGCCGGCACCACCGGCCCCTCACCCAACCCCAACAAC‑CCCCCAAAAAAAACCGGCCCCTTCATCGCAACCACCGCCACCAAGCCCCAGCCCATGcccc                              >  4:89978/1‑135 (MQ=255)
                                                                                                       gCCGTCCTGACCCCGAAGCCCCACCCCCCCCGGCGCCGGCACCCCCGCCCGCTCCCCCATCCCGTGCACC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCa                         <  3:6132/136‑1 (MQ=255)
                                                                                                         cGTCCTGATCACGAATCAGCACCCCCCCCCGCCCCGGCACCACCGGCCGCTCACCCCACCCGTTCAAC‑CCGGCAAAAAAAGCCGGCCGCTGCTGCCCCTCCAGCCCCCGCCAGCGCCCCCCCGTTCCCAGGGCAAt                       >  4:126563/1‑136 (MQ=255)
                                                                                                                   aCGAATCAGCACCCCACCCGGCGCCGGCACCACCCGCCGCTAACCCCTCCCCGTCAAC‑GCGCGCAAAAAAGCCCGCCGCAGCAGCCCCTCCCCCCCCCGCCACCACCAGCCCGTTCCCAGGGCAATCAGAACCCAg             >  4:46553/1‑136 (MQ=255)
                                                                                                                    cGAATCCGCACCCCCCCCGGCGCCGGCACCCCCGGCCGCGCACCCATCCCGTTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAgg            <  8:134709/136‑1 (MQ=255)
                                                                                                                             accccccccGGCGCCGGCACCACCGGCCGCTCACCCATCCCGTTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGGTGCGCCTCCACCGCCAGCAACCGCCCCCCCATGCCCACGGCAATCGCCCCCCAGGCTTTGCCa    >  1:10222/1‑135 (MQ=255)
                                                                                                                              ccccccccGGCGCCGGCACCACCGGCCGCTCACCCACCCCGTTCCCC‑GCGCCAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGt  <  3:202936/136‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCACCCATCCCGTTCAAC‑GCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGT  >  ACB122/4880220‑4880481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑