breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 44,293 Δ1 bp 100% intergenic (+124/‑367) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 91,899 T→G 17.6% L96V (TTG→GTG)  prlC → Oligopeptidase A
RA 120,975 Δ1 bp 100% intergenic (+272/+100) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 169,760 A→C 12.1% intergenic (+724/+2963) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,792 G→A 10.1% intergenic (+756/+2931) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,811 C→A 10.3% intergenic (+775/+2912) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,813 A→G 10.9% intergenic (+777/+2910) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,817 T→A 11.1% intergenic (+781/+2906) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,819 T→G 11.2% intergenic (+783/+2904) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,820 T→C 11.6% intergenic (+784/+2903) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,821 A→G 11.9% intergenic (+785/+2902) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 169,822 G→T 11.9% intergenic (+786/+2901) PP_RS08750 → / ← fhuA_1 tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor
RA 179,484 T→C 100% C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 258,568 T→C 5.8% L216P (CTG→CCG)  davT → 5‑aminovalerate aminotransferase DavT
RA 272,715 C→G 6.6% A194A (GCG→GCC yecS_1 ← L‑cystine transport system permease proteinYecS
RA 367,777 G→T 7.4% R6L (CGC→CTC)  fixB → Protein FixB
RA 392,749 G→C 7.2% intergenic (‑116/‑66) yihG ← / → CJ019_00361 putative acyltransferase YihG/hypothetical protein
RA 395,017 A→C 16.6% L187V (TTG→GTG)  yibF ← putative GST‑like protein YibF
RA 395,021 A→C 29.6% P185P (CCT→CCG yibF ← putative GST‑like protein YibF
RA 399,292 A→C 35.1% intergenic (‑186/+122) CJ019_00365 ← / ← aceF hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
RA 411,781 C→A 8.7% L172I (CTA→ATA)  kdkA_1 → 3‑deoxy‑D‑manno‑octulosonic acid kinase
RA 420,269 T→A 6.9% S194C (AGC→TGC)  fhuA_2 ← Ferrichrome outer membrane transporter/phagereceptor
RA 545,486 T→A 5.9% V46D (GTC→GAC)  rplW → 50S ribosomal protein L23
RA 575,740 T→G 23.9% F186V (TTT→GTT)  selB → Selenocysteine‑specific elongation factor
RA 709,887 G→A 7.5% G139S (GGC→AGC)  CJ019_00664 → hypothetical protein
RA 759,433 A→C 48.4% intergenic (+42/‑126) mmuM_1 → / → CJ019_00729 Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase
RA 812,764 G→T 6.0% R65L (CGT→CTT)  ada → Bifunctional transcriptional activator/DNArepair enzyme Ada
RA 924,333 T→G 25.8% D4780A (GAC→GCC)  CJ019_00881 ← hypothetical protein
RA 1,116,475 T→A 6.6% intergenic (‑59/+31) CJ019_01056 ← / ← capB_1 hypothetical protein/Cold shock protein CapB
RA 1,266,383 T→G 49.1% intergenic (+121/+47) cysK_1 → / ← CJ019_01185 Cysteine synthase/hypothetical protein
RA 1,313,943 A→C 33.3% L614R (CTG→CGG)  CJ019_01235 ← hypothetical protein
RA 1,313,950 T→G 35.1% R612R (AGG→CGG)  CJ019_01235 ← hypothetical protein
RA 1,317,342 C→A 14.6% A248S (GCA→TCA)  yfdC ← Inner membrane protein YfdC
RA 1,528,308 A→C 26.6% P87P (CCA→CCC argJ → Arginine biosynthesis bifunctional protein ArgJ
RA 1,563,328 C→T 5.9% S361S (AGC→AGT pcaF_1 → Beta‑ketoadipyl‑CoA thiolase
RA 1,567,244 C→T 7.0% D48D (GAC→GAT CJ019_01477 → hypothetical protein
RA 1,585,043 T→G 37.2% intergenic (‑94/‑109) rhaS_1 ← / → CJ019_01492 HTH‑type transcriptional activator RhaS/hypothetical protein
RA 1,645,364 C→T 7.4% V1487V (GTG→GTA shlA ← Hemolysin
RA 1,670,690 A→C 34.8% intergenic (+27/‑104) thrC_2 → / → CJ019_01569 Threonine synthase/hypothetical protein
RA 1,688,322 T→G 23.9% L411R (CTT→CGT)  wspC → putative biofilm formation methyltransferaseWspC
RA 1,720,951 T→G 33.4% D90A (GAC→GCC)  plsB ← Glycerol‑3‑phosphate acyltransferase
RA 1,806,731 G→T 52.8% K10N (AAG→AAT yeiG → S‑formylglutathione hydrolase YeiG
RA 1,807,474 G→A 19.3% G258D (GGC→GAC)  yeiG → S‑formylglutathione hydrolase YeiG
RA 1,890,959 T→G 25.9% E103D (GAA→GAC rdgC ← Recombination‑associated protein RdgC
RA 1,927,136 A→G 10.8% D182D (GAT→GAC minC ← Septum site‑determining protein MinC
RA 1,997,753 G→A 6.1% T348T (ACC→ACT CJ019_01900 ← hypothetical protein
RA 2,131,359 T→G 32.1% R135R (CGT→CGG yadG → putative ABC transporter ATP‑binding proteinYadG
RA 2,142,773 G→A 7.7% I268I (ATC→ATT rne ← Ribonuclease E
RA 2,203,886 A→C 35.2% T74P (ACC→CCC)  CJ019_02088 → putative protein
RA 2,241,556 T→G 24.0% N367T (AAC→ACC)  CJ019_02129 ← hypothetical protein
RA 2,296,924 C→T 8.6% Q325Q (CAG→CAA CJ019_02178 ← hypothetical protein
RA 2,350,451 G→A 7.3% intergenic (‑17/+92) CJ019_02223 ← / ← CJ019_02224 hypothetical protein/hypothetical protein
RA 2,380,000 T→G 16.3% N54T (AAC→ACC)  nrtA ← Nitrate/nitrite binding protein NrtA
RA 2,491,272 A→C 31.5% H33P (CAC→CCC)  iutA → Ferric aerobactin receptor
RA 2,553,519 T→G 19.0% W121G (TGG→GGG)  CJ019_02406 → hypothetical protein
RA 2,712,958 A→C 19.3% T83P (ACC→CCC)  CJ019_02561 → hypothetical protein
RA 2,723,474 G→A 6.4% A288A (GCG→GCA nepI_1 → Purine ribonucleoside efflux pump NepI
RA 2,791,318 C→A 9.3% P159P (CCG→CCT nucM ← Nuclease NucM
RA 2,829,804 A→G 6.2% L101P (CTG→CCG)  gabD1 ← Succinate‑semialdehyde dehydrogenase [NADP( )]1
RA 3,061,793 G→A 100% S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)
RA 3,244,200 G→A 8.3% A176A (GCG→GCA CJ019_03044 → hypothetical protein
RA 3,259,465 C→T 9.7% V32M (GTG→ATG)  CJ019_03060 ← hypothetical protein
RA 3,275,333 T→G 17.0% A30A (GCA→GCC CJ019_03079 ← hypothetical protein
RA 3,440,343 A→C 100% S18R (AGC→CGC)  CJ019_03266 → hypothetical protein
RA 3,617,883 T→G 44.2% D186A (GAC→GCC)  CJ019_03428 ← 2,6‑dihydropseudooxynicotine hydrolase
RA 3,648,351 G→A 5.8% Q367* (CAG→TAG)  gsiA_1 ← Glutathione import ATP‑binding protein GsiA
RA 3,661,125 C→A 6.6% G207C (GGC→TGC)  CJ019_03469 ← putative protein
RA 3,686,797 Δ1 bp 7.0% coding (2428/2502 nt) ligD ← Multifunctional non‑homologous end joiningprotein LigD
RA 3,775,251 A→C 19.7% D44A (GAC→GCC)  nikC → Nickel transport system permease protein NikC
RA 3,784,282 G→A 8.0% Q223Q (CAG→CAA CJ019_03594 → Formylglycine‑generating enzyme
RA 3,813,093 A→G 6.7% S43G (AGC→GGC)  CJ019_03616 → hypothetical protein
RA 3,848,001 A→C 22.1% F283V (TTT→GTT)  CJ019_03645 ← hypothetical protein
RA 3,905,078 A→C 29.2% E248A (GAG→GCG)  CJ019_03692 → hypothetical protein
RA 3,908,501 T→G 41.7% intergenic (‑70/‑176) rstA_2 ← / → acrE_2 Transcriptional regulatory protein RstA/Multidrug export protein AcrE
RA 3,909,205 A→C 20.9% T177P (ACC→CCC)  acrE_2 → Multidrug export protein AcrE
RA 3,934,892 A→C 19.3% V100G (GTG→GGG)  CJ019_03719 ← hypothetical protein
RA 3,957,351 G→A 100% H100Y (CAC→TAC)  dmlR_13 ← HTH‑type transcriptional regulator DmlR
RA 3,959,315 T→G 42.5% intergenic (+76/‑138) CJ019_03739 → / → rlhA_2 hypothetical protein/23S rRNA 5‑hydroxycytidine synthase
RA 3,986,622 T→G 31.2% D31A (GAT→GCT)  CJ019_03763 ← hypothetical protein
RA 4,029,454 A→C 23.4% D287A (GAC→GCC)  mcpU_3 → Methyl‑accepting chemotaxis protein McpU
RA 4,077,164 T→G 39.5% L173R (CTG→CGG)  artJ_2 → ABC transporter arginine‑binding protein 1
RA 4,080,182 T→G 23.3% R412R (CGT→CGG dadA → D‑amino acid dehydrogenase
RA 4,112,358 T→G 20.2% V468G (GTC→GGC)  iorB_2 → Isoquinoline 1‑oxidoreductase subunit beta
RA 4,131,763 T→G 22.7% E23A (GAG→GCG)  CJ019_03889 ← hypothetical protein
RA 4,135,592 A→C 11.9% N27T (AAC→ACC)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,135,607 A→C 26.5% Q32P (CAG→CCG)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,135,612 T→G 24.5% W34G (TGG→GGG)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,245,134 A→C 22.5% L182V (TTG→GTG)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,245,145 A→C 55.7% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,258,554 A→C 20.5% H41P (CAC→CCC)  pht2 → Phthalate 4,5‑dioxygenase oxygenase reductasesubunit
RA 4,500,158 T→C 6.2% L64P (CTG→CCG)  yqjG → Glutathionyl‑hydroquinone reductase YqjG
RA 4,502,376 A→C 40.5% V17G (GTC→GGC)  cysG_1 ← Siroheme synthase
RA 4,586,078 A→C 34.9% E230D (GAA→GAC accA1 → Acetyl‑/propionyl‑coenzyme A carboxylase alphachain
RA 4,626,159 C→A 19.9% intergenic (+117/+181) CJ019_04367 → / ← pgsA hypothetical protein/CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase
RA 4,656,288 A→C 16.6% D502A (GAC→GCC)  nuoG → NADH‑quinone oxidoreductase subunit G
RA 4,689,048 C→T 9.5% V295M (GTG→ATG)  CJ019_04426 ← hypothetical protein
RA 4,690,862 T→G 46.0% L281R (CTG→CGG)  yhaJ_2 → HTH‑type transcriptional regulator YhaJ
RA 4,710,264 C→T 7.4% S616S (AGC→AGT rcsC_16 → Sensor histidine kinase RcsC
RA 4,874,567 A→C 33.4% intergenic (+13/‑174) CJ019_04568 → / → uacT_1 hypothetical protein/Uric acid transporter UacT
RA 4,880,221 A→C 27.3% V96G (GTG→GGG)  CJ019_04574 ← hypothetical protein
RA 4,880,240 T→A 21.8% M90L (ATG→TTG)  CJ019_04574 ← hypothetical protein
RA 5,026,996 C→T 12.1% intergenic (+335/‑75) yabJ_3 → / → CJ019_03742 2‑iminobutanoate/2‑iminopropanoate deaminase/hypothetical protein
RA 5,066,751 T→A 5.6% K14* (AAA→TAA)  CJ019_04758 ← hypothetical protein
RA 5,073,255 G→T 7.7% intergenic (‑674/+518) CJ019_04767 ← / ← csrA2 Magnesium transporter MgtE/Translational regulator CsrA2
RA 5,149,443 G→A 5.8% A26T (GCC→ACC)  yhaJ_3 → HTH‑type transcriptional regulator YhaJ
RA 5,182,333 A→C 15.3% G130G (GGT→GGG CJ019_04876 ← hypothetical protein
RA 5,205,006 G→T 8.9% P257H (CCC→CAC)  eptA_2 ← Phosphoethanolamine transferase EptA
RA 5,284,713 T→G 20.5% F162C (TTT→TGT)  cmpR_6 → HTH‑type transcriptional activator CmpR
RA 5,290,634 G→A 6.3% T185T (ACC→ACT dgcN ← Diguanylate cyclase DgcN
RA 5,301,183 A→C 53.5% intergenic (‑106/+3467) recC ← / ← PP_RS08750 RecBCD enzyme subunit RecC/tRNA‑Val
RA 5,317,925 G→A 6.7% V245I (GTC→ATC)  CJ019_05003 → putative protein
RA 5,548,612 T→C 5.7% D18G (GAT→GGT)  hcp1_2 ← Protein hcp1
RA 5,669,107 T→C 7.3% K27R (AAG→AGG)  yceI ← Protein YceI
RA 5,689,794 T→G 48.2% R228R (CGT→CGG pyrC' → Dihydroorotase‑like protein
RA 5,689,824 T→G 45.1% R238R (CGT→CGG pyrC' → Dihydroorotase‑like protein
RA 5,695,008 C→T 12.3% S551F (TCC→TTC)  phaC_1 → Poly(3‑hydroxyalkanoate) polymerase subunitPhaC
RA 5,757,234 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein
RA 5,992,483 C→T 8.2% W166* (TGG→TGA ycaC_4 ← putative hydrolase YcaC
RA 6,016,995 T→G 51.9% intergenic (+75/+69) puuB_8 → / ← CJ019_05632 Gamma‑glutamylputrescine oxidoreductase/hypothetical protein
RA 6,021,519 A→C 36.0% L58R (CTG→CGG)  ycaD_4 ← putative MFS‑type transporter YcaD
RA 6,178,247 G→C 24.9% L614V (CTG→GTG)  mnmG ← tRNA uridine 5‑carboxymethylaminomethylmodification enzyme MnmG

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ KT2440 87974 88060 87 17 [14] [15] 18 [CJ019_00094]–[CJ019_00095] [CJ019_00094],[CJ019_00095]
* * ÷ KT2440 213560 213677 118 18 [15] [11] 22 [CJ019_00185] [CJ019_00185]
* * ÷ KT2440 346091 346223 133 17 [16] [16] 18 [btuD_1] [btuD_1]
* * ÷ KT2440 517190 517295 106 27 [14] [14] 18 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ KT2440 641246 641363 118 17 [16] [16] 17 [acoR_2] [acoR_2]
* * ÷ KT2440 711478 711559 82 18 [15] [15] 17 [thiO]–[aam] [thiO],[aam]
* * ÷ KT2440 1304746 1304852 107 17 [16] [16] 19 CJ019_01223/rapA putative signaling protein/RNA polymerase‑associated protein RapA
* * ÷ KT2440 1376289 1376338 50 17 [16] [15] 17 CJ019_01289 hypothetical protein
* * ÷ KT2440 1457225 1457334 110 18 [13] [16] 17 [algX] [algX]
* * ÷ KT2440 1499922 1500061 140 17 [16] [16] 20 [petC]–[sspA] [petC],[sspA]
* * ÷ KT2440 1585604 1585715 112 17 [14] [15] 18 CJ019_01493 hypothetical protein
* * ÷ KT2440 1608688 1608794 107 17 [16] [15] 17 CJ019_01513 hypothetical protein
* * ÷ KT2440 1697643 1697738 96 18 [14] [16] 17 [CJ019_01593] [CJ019_01593]
* * ÷ KT2440 1705085 1705189 105 17 [16] [14] 17 ppc/adk Phosphoenolpyruvate carboxylase/Adenylate kinase
* * ÷ KT2440 1914560 1914652 93 20 [15] [16] 17 [ppsC_1]–[CJ019_01830] [ppsC_1],[CJ019_01830]
* * ÷ KT2440 1923400 1923520 121 17 [15] [16] 17 [apeB] [apeB]
* * ÷ KT2440 2287020 2287129 110 17 [16] [16] 19 [CJ019_02168]–[CJ019_02169] [CJ019_02168],[CJ019_02169]
* * ÷ KT2440 2288824 2288926 103 17 [16] [16] 17 CJ019_02171/CJ019_02172 hypothetical protein/hypothetical protein
* * ÷ KT2440 2422917 2422993 77 17 [16] [16] 19 [ycbX] [ycbX]
* * ÷ KT2440 2467207 2467225 19 33 [4] [10] 35 CJ019_02325/tal hypothetical protein/Transaldolase
* * ÷ KT2440 3040201 3040304 104 17 [16] [16] 17 [pstB3] [pstB3]
* * ÷ KT2440 3046221 3046347 127 17 [16] [15] 20 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ KT2440 3103923 3104012 90 17 [16] [15] 22 [CJ019_02919] [CJ019_02919]
* * ÷ KT2440 3145290 3145390 101 18 [16] [15] 18 livH_4 High‑affinity branched‑chain amino acidtransport system permease protein LivH
* * ÷ KT2440 3769400 3769474 75 17 [14] [16] 17 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ KT2440 4163793 4163866 74 17 [16] [15] 17 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ KT2440 4346803 4346856 54 28 [15] [16] 21 nmoA Nitronate monooxygenase
* * ÷ KT2440 4523638 4523729 92 17 [16] [16] 17 [CJ019_04289] [CJ019_04289]
* * ÷ KT2440 4734437 4734536 100 17 [16] [16] 19 [CJ019_04468] [CJ019_04468]
* * ÷ KT2440 4846217 4846381 165 17 [16] [16] 17 recR/ybaB Recombination protein RecR/Nucleoid‑associated protein YbaB
* * ÷ KT2440 4883332 4883530 199 18 [16] [13] 18 [hyi]–[glxR] [hyi],[glxR]
* * ÷ KT2440 4944737 4944882 146 17 [16] [16] 19 [CJ019_04642] [CJ019_04642]
* * ÷ KT2440 5156292 5156448 157 17 [12] [16] 18 [CJ019_04852] [CJ019_04852]
* * ÷ KT2440 5188657 5188766 110 17 [16] [15] 17 [CJ019_04882]–[pxpC_2] [CJ019_04882],[pxpC_2]
* * ÷ KT2440 5193435 5193554 120 21 [16] [16] 20 [gltR_4]–[CJ019_04888] [gltR_4],[CJ019_04888]
* * ÷ KT2440 5250599 5250735 137 22 [13] [16] 19 [CJ019_04947]–[CJ019_04948] [CJ019_04947],[CJ019_04948]
* * ÷ KT2440 5331819 5331926 108 19 [12] [15] 17 [zraR] [zraR]
* * ÷ KT2440 5453344 5453446 103 18 [16] [16] 17 [lptE]–[holA] [lptE],[holA]
* * ÷ KT2440 5530837 5530938 102 18 [16] [16] 20 [nadE] [nadE]
* * ÷ KT2440 5551638 5551774 137 18 [16] [13] 17 CJ019_05227/purA hypothetical protein/Adenylosuccinate synthetase
* * ÷ KT2440 5727838 5727986 149 17 [16] [16] 17 [hutH] [hutH]
* * ÷ KT2440 5891553 5891653 101 18 [14] [16] 17 CJ019_05517/czcA_3 hypothetical protein/Cobalt‑zinc‑cadmium resistance protein CzcA
* * ÷ KT2440 5896919 5897041 123 19 [15] [16] 20 [CJ019_05521] [CJ019_05521]
* * ÷ KT2440 6074760 6074868 109 17 [16] [14] 17 [yeaD_2]–[CJ019_05690] [yeaD_2],[CJ019_05690]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? KT2440 2172773 =23 (0.410)9 (0.160) 8/232 NT 28.3% intergenic (‑50/+90) CJ019_02061/CJ019_02062 hypothetical protein/hypothetical protein
?KT2440 = 3965293 NA (NA)intergenic (+31/‑349) CJ019_03744/CJ019_00572 IS66 family transposase ISPpu14/IS110 family transposase ISPpu10
* ? KT2440 = 217278126 (0.470)8 (0.150) 7/232 NT 23.7% intergenic (‑58/+82) CJ019_02061/CJ019_02062 hypothetical protein/hypothetical protein
?KT2440 4479942 = NA (NA)intergenic (+171/‑99) CJ019_04248/CJ019_03742 hypothetical protein/hypothetical protein
* ? KT2440 4918800 =1 (0.020)77 (1.420) 44/228 NT 98.8% coding (7/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 = 5539492 NA (NA)intergenic (‑358/+71) CJ019_03622/rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
* ? KT2440 = 49188011 (0.020)46 (0.840) 29/230 NT 97.9% coding (6/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 5216408 = NA (NA)intergenic (‑50/+386) rhaS_5/CJ019_01212 HTH‑type transcriptional activator RhaS/IS110 family transposase ISPpu9
* ? KT2440 5066463 =61 (1.100)5 (0.090) 4/234 NT 7.6% coding (328/684 nt) CJ019_04758 hypothetical protein
?KT2440 = 5397613 NA (NA)coding (570/573 nt) dpkA_2 Delta(1)‑pyrroline‑2‑carboxylate/Delta(1)‑piperi deine‑2‑carboxylate reductase