breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 62,127 | +G | coding (1138/2907 nt) | rapA ← | RNA polymerase‑associated helicase protein |
RA | 547,694 | A→G | intergenic (+123/‑1156) | fdrA → / → ylbF | acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein |
RA | 547,835 | +G | intergenic (+264/‑1015) | fdrA → / → ylbF | acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein |
RA | 556,858 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional 5,10‑methylene‑tetrahydrofolatedehydrogenase/5,10‑methylene ‑tetrahydrofolatecyclohydrolase |
MC JC | 654,214 | Δ1,199 bp | insH_12 | insH_12 | |
RA | 987,574 | G→T | intergenic (‑170/+433) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
RA | 1,093,686 | T→C | V130A (GTA→GCA) | ycdT → | diguanylate cyclase |
RA | 1,197,797 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase,‑specific for NADP+ |
RA | 1,197,809 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase,‑specific for NADP+ |
MC JC | 1,301,041 | Δ1,336 bp | insC_8–insD_10 | insC_8, insD_10 | |
RA | 1,519,222 | G→T | V40V (GTG→GTT) | ydcX → | inner membrane protein |
RA | 1,669,601 | Δ1 bp | coding (678/1221 nt) | dgsA ← | DNA‑binding transcriptional repressor |
RA | 1,979,957 | G→T | intergenic (‑37/+398) | flhD ← / ← insH_10 | DNA‑binding transcriptional dual regulator/IS5 element protein |
RA | 2,005,401 | C→T | E115K (GAA→AAA) | fliC ← | flagellar filament structural protein |
MC JC | 2,176,983 | Δ1,199 bp | insH_11 | insH_11 | |
RA | 2,245,455 | A→G | S283P (TCG→CCG) | yeiB ← | inner membrane protein |
MC JC | 2,320,795 | Δ1,336 bp | insD_9–insC_5 | insD_9, insC_5 | |
RA | 2,382,028 | C→A | D79Y (GAC→TAC) | menH ← | peptidase |
RA | 2,474,595 | G→T | V7L (GTA→TTA) | gtrS → | inner membrane protein |
RA | 2,724,740 | C→A | noncoding (105/120 nt) | rrfG ← | 5S ribosomal RNA |
RA | 2,866,111 | A→G | intergenic (‑21/+159) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein |
MC JC | 3,261,134 | Δ1,199 bp | insH_16–[tdcD] | insH_16, [tdcD] | |
RA | 3,268,825 | A→G | T186A (ACA→GCA) | yhaC → | hypothetical protein |
RA | 3,449,813 | C→A | V507L (GTA→TTA) | rpoC ← | RNA polymerase, beta prime subunit |
MC JC | 3,742,936 | Δ1,336 bp | insD_8–insC | insD_8, insC | |
MC JC | 3,746,911 | Δ1,199 bp | insH_13 | insH_13 | |
MC JC | 3,750,591 | Δ1,199 bp | insH_14 | insH_14 | |
MC JC | 3,958,123 | Δ34 bp | coding (1156‑1189/1287 nt) | dctA → | C4‑dicarboxylic acid, orotate and citratetransporter |
RA | 4,038,630 | C→T | A101V (GCG→GTG) | ftsX → | transporter subunit |
RA | 4,150,184 | A→G | intergenic (+34/‑52) | pabA → / → argD | aminodeoxychorismate synthase, subunit II/bifunctional acetylornithineaminotransferase/succinyldiaminopimelate aminotransferase |
MC JC | 4,310,363 | Δ1,199 bp | insH_15–[alsK] | insH_15, [alsK] | |
RA | 4,342,252 | C→G | R119P (CGC→CCC) | adiY ← | DNA‑binding transcriptional activator |
RA | 4,371,274 | (A)5→3 | intergenic (‑9/+296) | dcuA ← / ← aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
RA | 4,371,467 | G→T | intergenic (‑202/+104) | dcuA ← / ← aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_007779_cfb | 1197818 | 1213115 | 15298 | 76 [75] | [75] 77 | [icd]–mcrA | [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,cohE,croE,ymfL,ymfM,ymfN,ymfR,ymfO,ymfP,ymfQ,stfP,tfaP,tfaE,pinE,mcrA |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_007779_cfb | 380484 = | NA (NA) | 119 (0.760) | 103/440 | 0.8 | NA | intergenic (‑418/‑46) | yaiO/insC_2 | hypothetical protein/IS2 insertion element repressor InsA |
? | NC_007779_cfb | = 381407 | NA (NA) | coding (510/906 nt) | insD | IS2 insertion element transposase InsAB' | |||||
* | ? | NC_007779_cfb | 381401 = | NA (NA) | 222 (1.440) | 163/436 | 0.0 | NA | coding (504/906 nt) | insD | IS2 insertion element transposase InsAB' |
? | NC_007779_cfb | = 381812 | NA (NA) | coding (303/387 nt) | yaiF | hypothetical protein | |||||
* | ? | NC_007779_cfb | = 3186081 | NA (NA) | 142 (0.920) | 115/438 | 0.5 | 96.6% | coding (26/2466 nt) | yqiG | outer membrane usher protein |
? | NC_007779_cfb | 3512753 = | 5 (0.030) | coding (529/1026 nt) | cytR | DNA‑binding transcriptional dual regulator | |||||
* | ? | NC_007779_cfb | = 3512759 | 7 (0.040) | 126 (0.810) | 100/438 | 0.9 | 94.8% | coding (535/1026 nt) | cytR | DNA‑binding transcriptional dual regulator |
? | NC_007779_cfb | = 3744262 | NA (NA) | intergenic (‑90/+31) | insC/yidZ | IS2 element protein/DNA‑binding transcriptional regulator | |||||
* | ? | NC_007779_cfb | = 3744263 | NA (NA) | 206 (1.320) | 163/440 | 0.0 | 98.6% | intergenic (‑91/+30) | insC/yidZ | IS2 element protein/DNA‑binding transcriptional regulator |
? | NC_007779_cfb | 3903274 = | 3 (0.020) | coding (37/825 nt) | bax | hypothetical protein | |||||
* | ? | NC_007779_cfb | = 3903279 | 6 (0.040) | 135 (0.870) | 115/438 | 0.5 | 95.8% | coding (42/825 nt) | bax | hypothetical protein |
? | NC_007779_cfb | = 4504190 | NA (NA) | intergenic (+10/‑89) | insD_3/yjgW | IS2 insertion element transposase InsAB'/hypothetical protein | |||||
* | ? | NC_007779_cfb | 3987185 = | NA (NA) | 193 (1.240) | 151/440 | 0.0 | 99.0% | intergenic (‑731/‑32) | slp/insH | outer membrane lipoprotein/IS5 transposase and trans‑activator |
? | NC_007779_cfb | 4366467 = | 2 (0.010) | coding (146/1539 nt) | cadC | DNA‑binding transcriptional activator | |||||
* | ? | NC_007779_cfb | = 3988378 | NA (NA) | 180 (1.160) | 138/438 | 0.1 | 97.8% | coding (747/783 nt) | yhiS | hypothetical protein |
? | NC_007779_cfb | = 4366471 | 4 (0.030) | coding (142/1539 nt) | cadC | DNA‑binding transcriptional activator | |||||
* | ? | NC_007779_cfb | = 4311556 | NA (NA) | 152 (0.980) | 110/438 | 0.6 | 99.4% | coding (922/1029 nt) | alsK | D‑allose kinase |
? | NC_007779_cfb | 4540915 = | 1 (0.010) | intergenic (+204/+379) | yjhR/nanS | frameshift suppressor/hypothetical protein |