Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,142,582 G→A H149Y (CAC→TAC)  ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,142,5820GA98.4% 205.0 / NA 64H149Y (CAC→TAC) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/28);  minor base C (0/1);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGACGCAGCTTACGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGG  >  CP009273/4142439‑4142726
                                                                                                                                               |                                                                                                                                                
tGGACGCAGCTTACGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGTATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCa                                                                                                                                             <  1:256729/149‑1 (MQ=255)
tGGACGCAGCTTACGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATCTCCTTGTGATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCa                                                                                                                                             <  2:615992/149‑1 (MQ=255)
tGGACGCAGCTTACGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCa                                                                                                                                             <  1:168983/149‑1 (MQ=255)
    cGCAGCTTACGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAgtgt                                                                                                                                         <  1:279608/149‑1 (MQ=255)
         cTTACGGATTTCATCGGTATGCCATGACTGGGAGATCAACTGGCGCAGGCGACGCATCTGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTCTCGAGCGGTTTTTAACAGGCGTTCACTTCCACCATTTGGTAGATCAGTGTatgac                                                                                                                                    >  2:318735/1‑149 (MQ=255)
           gtcGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACgg                                                                                                                                  <  1:61760/147‑1 (MQ=255)
            aCGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACggg                                                                                                                                 >  2:58393/1‑149 (MQ=255)
            aCGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACggg                                                                                                                                 <  2:3243/149‑1 (MQ=255)
                 tttCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAAtt                                                                                                                            <  1:797280/149‑1 (MQ=255)
                  ttCATCGGTATGCCAGGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAAttt                                                                                                                           <  1:422821/149‑1 (MQ=255)
                          tttGCCAGGACTGGGCGATCAATGCGCCCAGGCGTCGCAGCAGCTGGTTGTGTTCGGAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTggg                                                                                                                   <  2:162699/147‑1 (MQ=255)
                           aTGCCATGACTGGGCCATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGt                                                                                                                  <  1:121601/149‑1 (MQ=255)
                             gcttggccTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGa                                                                                                                <  2:382828/142‑1 (MQ=255)
                                     tGGTCGATCAACTGGCTCAGGCGACGCTTCAGCTGGTTGTGTTCTTAGTCAGCGATATCTTTTTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAg                                                                                                        <  2:210001/149‑1 (MQ=255)
                                       ggCGATGAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGa                                                                                                      <  1:282899/149‑1 (MQ=255)
                                        gCGATCAACTGGCGCAGGCGACGCAGCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGTGTAATTTCGGTTGGGTGAGCCGTGAGGAc                                                                                                     <  2:544838/149‑1 (MQ=255)
                                                 tGGCGCTGGCCACGCATCCGCTGGCTGTGTTCGTAGTCCGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGGGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCcag                                                                                            <  1:476294/149‑1 (MQ=255)
                                                     gcAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCcagcgac                                                                                        >  2:797728/1‑149 (MQ=255)
                                                     gcAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCcagcgac                                                                                        <  2:483027/149‑1 (MQ=255)
                                                              gCATCCGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGGTCACTTGCACCATTTTGTTGATCAGTGTACGACGGGGAATTTCGGTTGGGTGGGCCGTGAGGACCAGTTCCGGAGACAGCGCTTca                                                                               >  1:91780/1‑148 (MQ=255)
                                                              gCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCCTTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTcc                                                                               >  2:40837/1‑149 (MQ=255)
                                                                     cTGGTTGTGTTCGTAGTCAGCGATATCCGTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGCCGGGTAATCTCGTTTGGGTGAGCCGTGAGGTCCAGTTCCAGCAACAGCGATCACCCTGCtt                                                                        >  2:700528/1‑149 (MQ=255)
                                                                      tGGTTGTGTTCGGAGGAAGCGATGTCTACGTTATCGAGCTGTTTTAAACAGGCGTTCAGCTCCACCAATTTATAGAAGAGTGTCGGCCAGGGAGTTTCGCGGGGGTGCGCCGTGAGGACCAGTTCCAGCGACAGCGGTTCTACTGCttt                                                                       >  1:304115/1‑149 (MQ=255)
                                                                      gggTTGTGTTCGTAGTCAGCGGTATCTTTGTTGTCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGGTCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCttt                                                                       <  1:355234/148‑1 (MQ=255)
                                                                       ggTTGGGTTCGTAGTTAGCGATATCTTTGTTATCGACCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGTTCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCtttt                                                                      <  1:303124/149‑1 (MQ=255)
                                                                        gTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGGGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCttttt                                                                     >  2:34744/1‑149 (MQ=255)
                                                                            tgtTCGTAGTCAGCGATATTTTTGTTATCGGTCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACTGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGAt                                                                 <  1:499419/149‑1 (MQ=255)
                                                                              ttCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTc                                                                                >  1:740695/1‑132 (MQ=255)
                                                                              ttCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTc                                                                                <  2:740695/132‑1 (MQ=255)
                                                                                cGTAGTCAGCGATCTCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCCCCATTTTGTAGTTCAGTGTACGCCGGGCGATTTCGGTTGGGGGAGCCAGGAGGGCCAGTTCCAGCGCCAGCGATCCCCCGCCTTTCTtggtgggg                                                             >  1:35096/1‑147 (MQ=255)
                                                                                     tCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCAACTGCTTTTTTGATGGTGTCttg                                                        >  2:186065/1‑148 (MQ=255)
                                                                                       aGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGc                                                      >  2:146478/1‑149 (MQ=255)
                                                                                       aGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGc                                                      >  2:189070/1‑149 (MQ=255)
                                                                                              tCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTc                                               >  1:573920/1‑149 (MQ=255)
                                                                                               cTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGCTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTcc                                              >  1:464416/1‑149 (MQ=255)
                                                                                               cTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTcc                                              >  2:89869/1‑149 (MQ=255)
                                                                                               cTTTGTGATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTcc                                              <  2:730357/149‑1 (MQ=255)
                                                                                                tttGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCg                                             >  2:268485/1‑149 (MQ=255)
                                                                                                tttGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCg                                             >  1:812259/1‑149 (MQ=255)
                                                                                                tttGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCg                                             >  2:322503/1‑149 (MQ=255)
                                                                                                tttGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCg                                             >  2:863912/1‑149 (MQ=255)
                                                                                                tttGTTATCGAGCTGTTTTAAACAGGCATTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCg                                             >  2:585888/1‑149 (MQ=255)
                                                                                                 ttGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCgg                                            <  1:667475/149‑1 (MQ=255)
                                                                                                  tGTTCTCGAGCTGTTTTAAACAGGTGTATACCTCCACCATTTTGTAGATGAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGc                                           <  2:299657/149‑1 (MQ=255)
                                                                                                    ttATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTg                                         >  1:833431/1‑149 (MQ=255)
                                                                                                      aTCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGt                                       >  2:42808/1‑149 (MQ=255)
                                                                                                      aTCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGt                                       >  2:42763/1‑149 (MQ=255)
                                                                                                              gTTTTAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGt                               >  2:677408/1‑149 (MQ=255)
                                                                                                               ttttAAACAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGtt                              >  2:820967/1‑149 (MQ=255)
                                                                                                                  tgaaCTGGCGTTCATTTCCACCTTTTTGTAGTGCGGTGTACGTGGGGTTATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGGTGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTAc                           <  1:30690/147‑1 (MQ=255)
                                                                                                                  tAAACAGGCGTTCACTTCCACAATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGCCAGCGATTCCACTGCTTTTTTGATGGTGTCGTCGCTCAGTTCCGGCTGGTTGTTCAGTTTAc                           >  1:713126/1‑149 (MQ=255)
                                                                                                                  aaaaCAGGCCTTCACTTCAACCATTTTGTCGTTCAGGGTGCGACGGGTACTTTCGGTTGGGTGAGCCGTGAGGTCCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTAc                           <  2:733328/148‑1 (MQ=255)
                                                                                                                      cAGGCGTTCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAg                       >  2:575640/1‑149 (MQ=255)
                                                                                                                            ttCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCg                 >  2:343690/1‑149 (MQ=255)
                                                                                                                            ttCACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTATTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCCGGGGGCg                 >  1:19702/1‑149 (MQ=255)
                                                                                                                              cACTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTCAGCAGGGTGCggg               >  1:242483/1‑149 (MQ=255)
                                                                                                                               aCTTCCACCATTTTGTAGATCAGTGTACGACGGACAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCACTTTACGCGGGGAGCGGGc              >  1:55363/1‑149 (MQ=255)
                                                                                                                                cTTCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCg             >  2:397763/1‑149 (MQ=255)
                                                                                                                                 ttCCACCATTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGa            <  2:833431/149‑1 (MQ=255)
                                                                                                                                  tCCACCCTTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGAt           <  2:102837/149‑1 (MQ=255)
                                                                                                                                    caccaTTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCa         <  2:812259/149‑1 (MQ=255)
                                                                                                                                       caTTTTGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACtt      >  2:723374/1‑149 (MQ=255)
                                                                                                                                          tttGTAGATCAGTGTTCGACGGGTAATTTCGGTTGGGTGAGCCGTGGTGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTTTTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCg   <  1:34744/149‑1 (MQ=255)
                                                                                                                                           ttGTAGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCgg  >  2:811584/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TGGACGCAGCTTACGGATTTCATCGGTATGCCATGACTGGGCGATCAACTGGCGCAGGCGACGCATCAGCTGGTTGTGTTCGTAGTCAGCGATATCTTTGTTATCGAGCTGTTTTAAACAGGCGTTCACTTCCACCATTTTGTGGATCAGTGTACGACGGGTAATTTCGGTTGGGTGAGCCGTGAGGACCAGTTCCAGCGACAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGG  >  CP009273/4142439‑4142726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑