| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 2,667,677 | G→A | A124V (GCG→GTG) | yphB ← | mutarotase superfamily protein, YphB family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,667,677 | 0 | G | A | 96.4% | 174.6 / NA | 57 | A124V (GCG→GTG) | yphB | mutarotase superfamily protein, YphB family |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (24/30); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTC > CP009273/2667532‑2667822 | ccgCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATccaccg < 1:159922/147‑1 (MQ=255)cTGCTCCCGCCCCGCCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATccaccg < 1:30927/149‑1 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGTCTAAATCCGCGTTTGCGGCGACAACGAAAAATAAGGATGCCAGCCGGTACCAAACGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGCCAGCGTATCCACCGTTAAAt < 2:248075/149‑1 (MQ=255) gCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGa < 2:681346/149‑1 (MQ=255) cagccagTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGAGACAGAGGGCGGCACCGCCAGCGTATCCCCCGTTAAATGAAACg > 1:313740/1‑149 (MQ=255) ccagTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGACGTTAAATGAAACGCCt > 2:748623/1‑149 (MQ=255) gTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGAc < 1:154539/149‑1 (MQ=255) ccGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGAAGCCAGCCGGTACCAAATGCCAGCGTCTCTGCCCCTGGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGTAACGCCttacttac > 2:273220/1‑149 (MQ=255) tCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGCCTTACCCGa > 1:426943/1‑149 (MQ=255) tCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGa < 2:714715/149‑1 (MQ=255) gCCTGGGCCTGAACCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATa < 2:860314/149‑1 (MQ=255) gCCCGTTCCTGAACCCGCTTTTGCGGCGACAACGGAAAACAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATa < 1:127471/149‑1 (MQ=255) cTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGt < 2:324353/149‑1 (MQ=255) tgCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCCGACTTACCCGATAGTGAt > 1:652656/1‑149 (MQ=255) ggcTGATTACGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATa < 2:68764/147‑1 (MQ=255) gCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATa < 2:671572/149‑1 (MQ=255) cTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTCCCAAATGGCCGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATCAAACGCCTGACTTACCCGATAGTGATAGa > 2:360238/1‑149 (MQ=255) aaTCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTGACCGGCACCGCATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAAacac > 2:170887/1‑149 (MQ=255) tCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCg < 2:576623/149‑1 (MQ=255) ccGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGc > 1:422702/1‑149 (MQ=255) ccGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGc > 1:867170/1‑149 (MQ=255) gcgTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTg > 1:52157/1‑149 (MQ=255) gTTTGCGGCCACAACTGAAAACAAGGATGCCAGTCGGTAACAAATGGCAGCCTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCTACCGTTAAATGACACGCCTGACTTACCTGATAGTGATAGACACCGCTGCg < 1:363388/149‑1 (MQ=255) gcggcgACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGt < 1:44430/149‑1 (MQ=255) gcggcgACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGt > 2:809030/1‑149 (MQ=255) ggcgACAACGGAACATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTc < 2:683324/149‑1 (MQ=255) gcgACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa < 2:744018/149‑1 (MQ=255) gACAACGGAAAATAAGGATGCCAGCCGGTACTAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAAAGCGTCTCCGACAGCGTATCTGCGGTTAAATCAAACACCCGACTTACCCGATAGTGTAAGGCCCCGCAGCGATGGTCATa > 1:427029/1‑149 (MQ=255) cGGAAAATAAGGATGCCAGCCGGTAGCAAAAGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGGCAGCGTATCCACCGCTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCTCTGCGATGTTCATACACCa > 2:463222/1‑149 (MQ=255) cGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCTCCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCa < 1:173634/149‑1 (MQ=255) cGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCa < 2:154677/149‑1 (MQ=255) aaaaTAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGac < 2:382187/149‑1 (MQ=255) aaaaTAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGaa > 2:221204/1‑148 (MQ=255) aaaTAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTAATTGGTGACAGAGAGCGTCACCGTCAGCGGATCCACCGTTAAATGAAACGCCTGACTTATCCGATAGTGATAGACACCGATGCGAGGATCGTACACAAGgca > 2:223179/1‑149 (MQ=255) aaGGATGCCAGCCGGTACCAAATGGCAGCGTCCCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACaaa < 2:94424/149‑1 (MQ=255) ggcccAAATGGCAGCGTCTCTGCCCCTTGAGTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGCGATAGACACCCCTGCGATGTTCATACACCAGACACAAACTATCATCGCtgtg < 2:59111/146‑1 (MQ=255) gTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGCTGTTCATACACCAGACACAAACTATCATCGCtgtg > 1:91939/1‑149 (MQ=255) ccAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCga < 1:439949/149‑1 (MQ=255) aagcggCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgaga < 1:273220/145‑1 (MQ=255) aactGGCAGCGTCTCTGCCCCCTGATTGGGGCCAGAGAGCGACACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGCTAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgcga < 1:748623/146‑1 (MQ=255) aaaTGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgaga < 2:426943/149‑1 (MQ=255) aaaTGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgaga > 2:636875/1‑149 (MQ=255) aaTGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACCCCGCTGCGATGTTCATACACCAGACACAAACTATGATCGCTGGCCGAGac > 1:738197/1‑149 (MQ=255) cAGCGTCCCTGCCCCTTGATTGGTGACAGAGAGCGGCCCCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTTCCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACAtt < 1:542584/149‑1 (MQ=255) tgcgggtctGCCGCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTCAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTg < 1:182449/143‑1 (MQ=255) gCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGc > 2:173842/1‑149 (MQ=255) tctGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATCAACAAGCCTGTTAGCTTCCTGCACCAGCCACAAACTATCATCGCTGTGCGAGAGACATTGCCACTc > 2:428689/1‑149 (MQ=255) tctGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTACCATCGCAGTGAGACACACATTGCCAccc > 1:577995/1‑147 (MQ=255) tctGCCCCTTGATTGGTGACAGAGAGCGGCACCGTCAGCGTATCCACCGTTAAATGAATCGCATGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTc > 2:536065/1‑149 (MQ=255) ctGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGAGAGACACATTGCCACTCg < 1:575087/149‑1 (MQ=255) cTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCccagc > 2:731921/1‑149 (MQ=255) gACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCACCGc > 1:617600/1‑149 (MQ=255) aCAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGcc > 1:566717/1‑149 (MQ=255) cAGAGAGCGGCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCg < 2:859055/149‑1 (MQ=255) aGCGTATCCACGGTTAAACTAAACGCGTGACGTGCCGGAGGGTGATAGGCGGCGGTGGGATGTTCATACACCAGCCACAAGCTTTCATCGCTGTGCGAGACACATTGCCAGTCGCCGAGCCAGCCATCGCCGTGTAGATAGTGCGCATc < 1:809030/149‑1 (MQ=255) tcGTATCCACCGTTAAATGAAACGCCTGACTTAACCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATcc < 1:360238/148‑1 (MQ=255) gCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATcc < 2:488965/149‑1 (MQ=255) aTCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCGCt > 1:663741/1‑149 (MQ=255) tCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTc > 2:488228/1‑149 (MQ=255) | CTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCACTC > CP009273/2667532‑2667822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |