Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,718,735 G→T P124Q (CCG→CAG)  kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,718,7350GT98.1% 160.9 / NA 53P124Q (CCG→CAG) kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (22/29);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGC  >  CP009273/2718590‑2718879
                                                                                                                                                 |                                                                                                                                                
tCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAAATTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGCGCGAGAAGCAATAATGCCTGAg                                                                                                                                               >  2:299962/1‑149 (MQ=255)
  cgccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGcc                                                                                                                                             >  2:729107/1‑149 (MQ=255)
   gccgATCAACGTCACATACTGAAATGATGCGTAAAAACCTATGCGCCCTTCAACGAGAATGGCACGCAGATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAACCGGGCGAGAAGCAATAATGCCGGGGcgc                                                                                                                                            >  2:350637/1‑147 (MQ=255)
       aTCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCCCGt                                                                                                                                        >  1:672952/1‑149 (MQ=255)
           aCGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCt                                                                                                                                    >  2:179703/1‑149 (MQ=255)
             gTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCtat                                                                                                                                  <  1:868278/149‑1 (MQ=255)
                   tACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTc                                                                                                                            <  1:291632/149‑1 (MQ=255)
                   tACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTc                                                                                                                            >  1:642426/1‑149 (MQ=255)
                      tGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGGGCGAGAGGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATa                                                                                                                         >  1:685692/1‑149 (MQ=255)
                       gAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATaa                                                                                                                        <  1:403857/149‑1 (MQ=255)
                           tgatgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTg                                                                                                                    <  1:501211/149‑1 (MQ=255)
                              tgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCACTGGTGCCATATTCTCCGCCAACAGAGAATCCCTGAGATAAACGCGCGAGAAGCAATATGGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGa                                                                                                                 <  2:128417/149‑1 (MQ=255)
                               gCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGag                                                                                                                <  1:277741/149‑1 (MQ=255)
                                 gTAAAAGCCGTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggc                                                                                                              <  1:734775/149‑1 (MQ=255)
                                  tAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGAaggca                                                                                                             >  2:792799/1‑149 (MQ=255)
                                   aaaaaCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCGATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATGGTTTCATAACCTggagagcag                                                                                                            >  1:463764/1‑142 (MQ=255)
                                    aaatCCTTTGCGCCCTTCAACGGAACCGTCCCTCATATAGGTGGCGCTGGTGCCATATTCTCCGGCAACAGATAATCCCTGAAATAACCGATCGAGAAGCCATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcagg                                                                                                           <  1:391010/149‑1 (MQ=255)
                                     acaCCTTTGCGCCCTTCAACGGCAACTTCACTAATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGaggcaggc                                                                                                          <  1:295562/147‑1 (MQ=255)
                                              cgcCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg                                                                                                 <  1:465284/149‑1 (MQ=255)
                                                 ccTTCAACGGCAACTTCACTCATATAGGGGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATACACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGa                                                                                              <  1:435672/149‑1 (MQ=255)
                                                  cTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGAt                                                                                             >  2:7600/1‑149 (MQ=255)
                                                      aaCGCCAACTTCACTCATATAGGGGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGa                                                                                         <  1:19527/149‑1 (MQ=255)
                                                       aCGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa                                                                                        <  2:154698/149‑1 (MQ=255)
                                                             aCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGAGACCCGCGacacct                                                                                  >  1:841123/1‑147 (MQ=255)
                                                               ttCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatca                                                                                <  1:7600/149‑1 (MQ=255)
                                                                   cTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCCACAGATAATCCCAGAAATAAACGCGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATaca                                                                            <  2:412026/149‑1 (MQ=255)
                                                                    tCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGCTCCGAAACACATCATacac                                                                           >  2:100145/1‑149 (MQ=255)
                                                                      atatAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac                                                                         <  1:690868/149‑1 (MQ=255)
                                                                        ataGGTGGCGCTTGTGCCATATTCTGCGCCAACAGAGAGTCCCTGAAATAAACGAGCGAGAAGCAATACTGCCCGCGCCCACGTACCTATATTTTCATAACCTGGTAGGCAGGCGATAACCAGCGATCCGAAACACATCATACTCACCg                                                                       <  2:528686/149‑1 (MQ=255)
                                                                          aGGTCGCGCTGGTGCCATATTCCCCGCCAACACATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt                                                                     <  2:328399/149‑1 (MQ=255)
                                                                            gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAACCACATCATACACACCGATac                                                                   >  2:891798/1‑148 (MQ=255)
                                                                            gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATaa                                                                   >  2:673752/1‑149 (MQ=255)
                                                                                cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGc                                                               >  1:886832/1‑149 (MQ=255)
                                                                                 gcTGGTGCCATATTCTCCGCCAACAGATAAACCCAGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAGCACATCATACACACCGATAACAGCa                                                              <  1:766494/149‑1 (MQ=255)
                                                                                  cTGGTCCCATATGCGCCGCCAACGTATAATCCCTGAAATAAACGAGCGACAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCAt                                                             <  1:77001/149‑1 (MQ=255)
                                                                                    ggTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAGACACATCATACACACCGATAACAGCATCg                                                           <  2:543619/149‑1 (MQ=255)
                                                                                         cATATTCTCCGCCGACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAtttt                                                      >  1:544526/1‑149 (MQ=255)
                                                                                           tatTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGATTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGCTAACAGCGTCGCtttttt                                                    >  1:255346/1‑149 (MQ=255)
                                                                                            atTCGCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAAGGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTg                                                   <  1:484202/149‑1 (MQ=255)
                                                                                             ttCTCCGCCAACAGATAAGCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGc                                                  <  2:57281/149‑1 (MQ=255)
                                                                                                tcCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGAc                                               >  1:557759/1‑149 (MQ=255)
                                                                                                   gCCAACAGATAATCCCTGAAATAAACGGGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGTCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCAt                                            <  2:393095/149‑1 (MQ=255)
                                                                                                   gCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAACCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAGCAGCATCGATTTTTTGCGACCAt                                            <  1:612799/149‑1 (MQ=255)
                                                                                                   gCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGAAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACAAGCGATCCGAAACACATCATACACACCGATAACAGCATCGGTTTTTTGCGACCAt                                            >  1:250649/1‑149 (MQ=255)
                                                                                                                 ccTGAAATAAACGAGCGAGAAGCGATAACGCCTGAGCCCACGTACCTATAGTTACATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATg                              >  2:493155/1‑149 (MQ=255)
                                                                                                                  cTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGAACCGAAACACATCATACACACCGATAACAGCATAGCTTTTTTGCGACCATGTTTATCGGCTATGc                             >  2:323650/1‑149 (MQ=255)
                                                                                                                       aTAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTCCCTATAGTGTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCa                        >  2:880005/1‑149 (MQ=255)
                                                                                                                              ggcgggAATCAATAATGCCTGAGGCGCCGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGcc                 <  1:85807/148‑1 (MQ=255)
                                                                                                                                   gaAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCg            >  1:876988/1‑149 (MQ=255)
                                                                                                                                    aaGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTGGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGc           <  2:332208/149‑1 (MQ=255)
                                                                                                                                      gaaataacGCCTGAGCCCCCGTACCTAGAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCt         <  2:241195/147‑1 (MQ=255)
                                                                                                                                         ataatGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACATCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTAtt      <  1:79058/149‑1 (MQ=255)
                                                                                                                                             tGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGgc  <  2:488677/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGC  >  CP009273/2718590‑2718879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑