Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,719,426	T→C	intergenic (‑321/+2)	kgtP ← / ← rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,719,426	0	T	C	100.0%	198.7 / NA	65	intergenic (‑321/+2)	kgtP/rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/26);  total (39/26)

TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566                                                                                                                                              |                                                                                                                                              tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCt                                                                                                                                        <  1:841341/149‑1 (MQ=255) tGGAAGTGGGCAAGTTGGGGAAGCCATATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCt                                                                                                                                        >  2:324852/1‑149 (MQ=255)   gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCCgg                                                                                                                                      >  1:343177/1‑149 (MQ=255)            aaGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCt                                                                                                                             <  2:465343/149‑1 (MQ=255)             aGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCACAAAGGCCATCCGTCAGGATGGCCTCTCGCATAATTCGATGCCTGGCAGTCCCCtc                                                                                                                            >  1:350208/1‑148 (MQ=255)             aGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCGGGATGGCCTTTCGCATCATTCGATGCCTGGCAGTTcccga                                                                                                                            >  1:400548/1‑147 (MQ=255)             aGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTGCCTa                                                                                                                            >  1:890491/1‑149 (MQ=255)               ttGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTAct                                                                                                                          >  2:157401/1‑149 (MQ=255)                   ggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCg                                                                                                                      >  2:304522/1‑149 (MQ=255)                    gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                     >  2:148348/1‑149 (MQ=255)                    gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                     >  2:27287/1‑149 (MQ=255)                       gCCGTATCCGTTGCTGAATCTGGTATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                  <  2:661480/149‑1 (MQ=255)                       gCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                  >  2:351044/1‑149 (MQ=255)                       gCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                  <  1:884577/149‑1 (MQ=255)                         cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGCTGGCCTTGCGCATAATTCGATGCCTGGCAGTTCCCCACTCTCGCATggg                                                                                                                <  2:639685/149‑1 (MQ=255)                         cGTACCCGTTGCTGAATCTGGCATATGTGGGAGGATACGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATggg                                                                                                                <  2:304358/149‑1 (MQ=255)                                gTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAccc                                                                                                         >  2:840441/1‑149 (MQ=255)                                    ctgcatacgcgacatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                     >  1:233831/14‑149 (MQ=25)                                    cTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                     >  1:631916/1‑149 (MQ=255)                                     taaaTCTGGCATATGTGAGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                                                                    <  2:177697/147‑1 (MQ=255)                                        aTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAc                                                                                                 >  2:603031/1‑149 (MQ=255)                                         tCTGGCATATGTGGGAGTATAAGAAGCGGAGGGTCGAATCTGGCTTTGTTTGAGGCTTGAATGCAAAAGGGCGATCGTTCATGATGGCGCTTCGCTTAATTCGAAGCCTGGCAGTTGCCTACTCTCGCATGGGGGGACCCCACaccacc                                                                                                >  1:178988/1‑149 (MQ=255)                                            gggctatGTGGGGGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCTGGCTGGCCTTTCGCATATTTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACCCTACCATc                                                                                             <  1:351068/145‑1 (MQ=25)                                            ggCATATGTGGGAGTATAAGCCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATc                                                                                             <  1:166409/149‑1 (MQ=255)                                              cATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCgg                                                                                           <  1:775848/149‑1 (MQ=255)                                                   gtgGGCGTATAAGACGCGCAGCGGCGCATCAGGCATTTTTCTCTGCCCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAAGTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                      <  1:262122/149‑1 (MQ=25)                                                   gtgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGACAATGCAAAAATGCCATCCGACAGGCTGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGGGACACCACACTACCCTCGGCGCTa                                                                                      >  1:399888/1‑149 (MQ=37)                                                   gtgGGAGGATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                      >  1:841789/1‑149 (MQ=255)                                                    tgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCACGATTGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCCTCCGCGCTAc                                                                                     >  1:412894/1‑149 (MQ=25)                                                     gggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGGCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGCGACCCCACACTACCCTCGGCGCTACg                                                                                    >  1:357464/1‑149 (MQ=255)                                                        aGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCg                                                                                 >  1:516390/1‑149 (MQ=255)                                                           ataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                              >  2:620099/1‑149 (MQ=255)                                                             aaGCCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCACTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                                                            <  1:557714/149‑1 (MQ=255)                                                             aaGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGCCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTGCCCTAGCCTCGCATGGGGAGTCCACACAGTACCATCGGCGCTACGGCGTTTCa                                                                            >  2:74982/1‑149 (MQ=21)                                                              aGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTAAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACTCGACGCTACCGTCGGCGCTACGGCGGTTCAc                                                                           >  2:272168/1‑149 (MQ=21)                                                               gACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGCCCCCCCACTACCATCGCCGCTCCGCCGTTTCACt                                                                          >  1:406411/1‑149 (MQ=25)                                                                aCGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACGGCGTTTCACtt                                                                         >  1:74080/1‑149 (MQ=255)                                                                    gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                     >  2:724790/1‑149 (MQ=255)                                                                               tCAGGCATTTTTTTCGGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑gg                                                         <  2:426683/149‑1 (MQ=255)                                                                                cAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGTTAT‑ggg                                                        <  1:241319/149‑1 (MQ=255)                                                                                cAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGAGGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑ggg                                                        <  1:17163/149‑1 (MQ=255)                                                                                      tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAgg                                                  >  1:823204/1‑149 (MQ=255)                                                                                      tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTCCACTTCTGAGTTCGGCAT‑GGGGTCAgg                                                  >  1:241615/1‑149 (MQ=255)                                                                                       ttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGt                                                 <  1:157401/149‑1 (MQ=255)                                                                                         ttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTgg                                               <  1:27287/149‑1 (MQ=255)                                                                                                 gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACcg                                       <  1:603031/149‑1 (MQ=255)                                                                                                 gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGGCAGGTGGGACCACcg                                       >  2:714742/1‑149 (MQ=255)                                                                                                    aaTGCAGAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCCACGCAGGTTCACTCCTGAGCTCGGCCTGGGGGTCAAGTGGGcccgccccc                                     >  1:22543/1‑146 (MQ=17)                                                                                                         aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACgg                               >  2:858338/1‑149 (MQ=255)                                                                                                         aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGAGTTTGACTTCTTAGTTCGGCAT‑GGGGTCAGTTGGGACCACCGCGCTACgg                               >  1:757597/1‑149 (MQ=17)                                                                                                              ggCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGGCCACCGAGCTACGtccgcc                          >  1:749007/1‑149 (MQ=255)                                                                                                                ccATCCGCCAGGAGGGCCTGTCGCATAACTCGATGCCGGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        <  2:441762/149‑1 (MQ=14)                                                                                                                  accggTCAGGATGGCCTTTCGCCTAAGTCGATGCCTGGCAGTTCCCTACTCTTGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGc                      <  1:556410/145‑1 (MQ=14)                                                                                                                          ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTcag              >  2:289539/1‑147 (MQ=255)                                                                                                                          ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTg              <  2:51549/149‑1 (MQ=255)                                                                                                                          ggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTg              >  1:228866/1‑149 (MQ=255)                                                                                                                           gATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt             <  2:418076/149‑1 (MQ=255)                                                                                                                               gCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTa         <  2:516390/149‑1 (MQ=38)                                                                                                                                ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        >  2:440281/1‑149 (MQ=37)                                                                                                                                ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCAGAGTTCGGCAT‑GGGGTCAGGTGCGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        <  1:546778/149‑1 (MQ=255)                                                                                                                                 cTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATc       >  2:524324/1‑149 (MQ=25)                                                                                                                                  tttCGCATAATTCGATGCCTGGCAGTTCCCGACTCTCGCATGGGGAGACACCACACTACCATCGGCGCTACGGCGTTTCTCTTCTGAGTTCGGCAT‑GGGGTCAGGGGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGCTTTATCa      >  2:221351/1‑149 (MQ=11)                                                                                                                                    tCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGa    <  2:233831/149‑1 (MQ=18)                                                                                                                                    ccGCATAATTCGATGCCTGGCATTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGa    <  1:809095/148‑1 (MQ=17)                                                                                                                                      gCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCAGCGCTACGCCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTCCGGCCGCCAGGCAAATGCCGTTTTATCATAcc  >  1:327879/1‑149 (MQ=11)                                                                                                                                              |                                                                                                                                              TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAT‑GGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑