breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
JC 89,331 Δ3 bp 100% coding (1432‑1434/1767 nt) ftsI → transpeptidase involved in septal peptidoglycan synthesis (penicillin‑binding protein 3)
JC 360,104 183 bp x 2 61.0% duplication lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor
MC JC 776,903 Δ208 bp 100% lysQ lysQ
RA 822,078 C→T 5.3% G211S (GGC→AGC)  ybhS ← inner membrane putative ABC superfamily transporter permease
RA 1,169,841 G→C 5.7% R261G (CGA→GGA)  ycfT ← inner membrane protein
RA 1,191,676 C→T 11.8% H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T 14.7% T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,701 T→C 14.3% L375L (TTA→CTA) ‡ icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,703 A→G 18.1% L375L (TTA→TTG) ‡ icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,733 C→T 11.9% N385N (AAC→AAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,736 G→C 13.3% A386A (GCG→GCC icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,739 A→G 14.2% K387K (AAA→AAG icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,748 C→T 13.7% T390T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,760 G→A 12.4% E394E (GAG→GAA icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,772 T→A 11.7% D398E (GAT→GAA icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,546,067 C→T 5.6% A158V (GCG→GTG)  fdnI → formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible
RA 1,562,775 G→A 100% G102G (GGC→GGT yddW ← liprotein, glycosyl hydrolase homolog
RA 1,594,122 T→C 8.6% R115R (AGA→AGG lsrK ← autoinducer‑2 (AI‑2) kinase
RA 1,601,762 C→A 41.1% P54T (CCT→ACT)  tam → trans‑aconitate methyltransferase
RA 1,692,653 A→G 5.6% L262S (TTG→TCG)  malI ← transcriptional repressor of Mal regulon
RA 2,328,514 G→T 5.3% P537Q (CCG→CAG)  yfaA ← DUF2138 family protein, putative host defense protein
RA 3,529,154 G→T 100% A25D (GCC→GAC)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR
RA 4,368,791 C→A 32.3% intergenic (‑30/+33) ampC ← / ← frdD penicillin‑binding protein; beta‑lactamase, intrinsically weak/fumarate reductase (anaerobic), membrane anchor subunit

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 1203246 =102 (1.060)6 (0.070) 4/324 NT 7.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 63 (0.720)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 1203261102 (1.060)11 (0.130) 10/324 NT 12.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 63 (0.720)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 3610675 =85 (0.880)4 (0.040) 4/340 NT 5.2% coding (301/765 nt) nikD nickel transporter subunit
?CP009273 3610774 = 66 (0.720)coding (400/765 nt) nikD nickel transporter subunit
* ? CP009273 3851553 =117 (1.210)6 (0.070) 5/330 NT 5.8% coding (212/1494 nt) yidJ sulfatase/phosphatase superfamily protein
?CP009273 3851602 = 87 (0.970)coding (163/1494 nt) yidJ sulfatase/phosphatase superfamily protein