breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA CP009273 155,132 C→G R161P (CGC→CCC)  pcnB ← poly(A) polymerase
RA CP009273 188,740 C→G I133M (ATC→ATG pyrH → uridylate kinase
RA CP009273 407,471 C→T W156* (TGG→TAG)  araJ ← arabinose‑inducible putative transporter, MFS family
RA CP009273 1,782,894 C→A A68E (GCA→GAA)  aroH → 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible
MC JC CP009273 2,400,028 Δ9 bp coding (85‑93/939 nt) lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA CP009273 3,208,093 T→C F563S (TTC→TCC)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA CP009273 3,466,500 G→A T125I (ACC→ATC)  fusA ← protein chain elongation factor EF‑G, GTP‑binding
RA CP009273 3,547,518 G→A E359K (GAA→AAA)  malT → mal regulon transcriptional activator
RA CP009273 3,729,347 G→A E337K (GAG→AAG)  xylR → xylose divergent operon transcriptional activator
RA CP009273 3,933,885 G→T L30I (CTT→ATT)  hsrA ← putative multidrug or homocysteine efflux system
RA CP009273 4,173,770 T→G D866E (GAT→GAG rpoB → RNA polymerase, beta subunit
RA CP009273 4,224,841 C→T H386Y (CAC→TAC)  pgi → glucosephosphate isomerase
JC Exported 918 Δ8 bp intergenic (‑126/‑86) aadA ← / → fdh aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1800618 1801543 926 20 [15] [16] 22 [pfkB] [pfkB]
* * ÷ CP009273 1929101 1930590 1490 18 [16] [16] 19 [zwf] [zwf]
* * ÷ CP009273 4097464 4098436 973 18 [14] [16] 21 [pfkA] [pfkA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 19796 =NA (NA)842 (0.040) 156/230 0.0 37.6% noncoding (768/768 nt) IS1 repeat region
?Exported = 906 1399 (0.030)intergenic (‑114/‑105) aadA/fdh aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag
* ? CP009273 = 20563NA (NA)971 (0.050) 162/230 0.0 25.3% noncoding (1/768 nt) IS1 repeat region
?Exported 899 = 2873 (0.070)intergenic (‑107/‑112) aadA/fdh aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag
* ? CP009273 360104 =33 (0.470)25 (0.380)
+AATTCC
18/218 2.1 44.8% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 4033989 = 32 (0.460)noncoding (113/120 nt) rrfA 5S ribosomal RNA of rrnA operon
* ? CP009273 360104 =33 (0.470)10 (0.010)
+AATTCCCC
9/214 2.6 24.6% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?KX077536 8127 = NA (NA)intergenic (+52/–) unknown/– cat/–
* ? CP009273 3576788 =NA (NA)93 (1.330) 50/230 0.2 88.6% intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
?CP009273 = 4232212 12 (0.170)intergenic (‑30/+342) xylE/malG D‑xylose transporter/maltose transporter subunit
* ? CP009273 = 3577555NA (NA)91 (1.300) 54/230 0.1 95.8% intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
?CP009273 4232204 = 4 (0.060)intergenic (‑22/+350) xylE/malG D‑xylose transporter/maltose transporter subunit
* ? CP009273 4532499 =10 (0.140)62 (0.960) 46/212 0.2 84.2% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 14 (0.220)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 = 453250710 (0.140)65 (1.010) 41/212 0.3 84.9% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 = 4532803 14 (0.220)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)