breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 42,566 C→A 5.5% A55E (GCG→GAG)  fixA → anaerobic carnitine reduction putative electron transfer flavoprotein subunit
RA 48,827 G→A 5.3% E353E (GAG→GAA kefC → potassium:proton antiporter
RA 75,208 G→A 11.9% G367G (GGG→GGA setA → broad specificity sugar efflux system
RA 75,590 G→T 5.7% N117K (AAC→AAA leuD ← 3‑isopropylmalate dehydratase small subunit
RA 89,224 G→A 8.0% R442H (CGC→CAC)  ftsI → transpeptidase involved in septal peptidoglycan synthesis (penicillin‑binding protein 3)
RA 145,878 C→A 8.7% A71S (GCC→TCC)  panB ← 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
RA 171,709 C→T 8.0% A39V (GCG→GTG)  clcA → H(+)/Cl(‑) exchange transporter
RA 189,173 G→T 8.1% intergenic (+106/‑186) pyrH → / → frr uridylate kinase/ribosome recycling factor
RA 222,173 T→C 6.2% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA of rrnH operon
RA 247,671 G→A 6.1% R96H (CGC→CAC)  dinB → DNA polymerase IV
RA 249,874 G→C 15.1% intergenic (+226/‑92) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 249,908 A→G 17.9% intergenic (+260/‑58) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 258,104 C→T 8.3% G297G (GGC→GGT proA → gamma‑glutamylphosphate reductase
RA 262,526 C→T 9.5% pseudogene (153/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 262,529 C→T 10.2% pseudogene (150/192 nt) ykfK ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related
RA 263,285 G→A 7.7% D144D (GAC→GAT yafZ ← CP4‑6 prophage; conserved protein
RA 263,333 G→C 5.7% L128L (CTC→CTG yafZ ← CP4‑6 prophage; conserved protein
RA 267,804 G→A 5.2% pseudogene (330/426 nt) insN → pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 335,776 C→T 9.1% intergenic (+322/‑100) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 350,026 C→A 8.3% A537E (GCG→GAG)  prpE → propionate‑‑CoA ligase
RA 381,744 G→T 12.2% E28* (GAG→TAG)  tauB → taurine transporter subunit
RA 423,515 A→T 6.6% D138V (GAT→GTT)  secD → SecYEG protein translocase auxillary subunit
RA 423,517 A→C 6.6% M139L (ATG→CTG)  secD → SecYEG protein translocase auxillary subunit
RA 423,521 Δ1 bp 6.9% coding (419/1848 nt) secD → SecYEG protein translocase auxillary subunit
RA 423,524 C→G 7.0% T141S (ACC→AGC)  secD → SecYEG protein translocase auxillary subunit
RA 445,212 C→T 100% G296S (GGT→AGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 446,280 C→A 5.7% V263L (GTG→TTG)  cyoA ← cytochrome o ubiquinol oxidase subunit II
RA 478,554 C→A 13.2% G436W (GGG→TGG)  acrB ← multidrug efflux system protein
RA 519,282 C→G 5.4% S189C (TCT→TGT)  rhsD → Rhs family putative polymorphic toxin
RA 523,498 C→A 7.7% pseudogene (91/708 nt) rhsH → pseudogene, Rhs familly protein
RA 573,883 G→C 9.1% T107T (ACG→ACC
R34P (CGG→CCG) 
rzpD →
rzoD →
DLP12 prophage; putative murein endopeptidase
DLP12 prophage; putative lipoprotein
RA 625,575 G→C 8.7% D76H (GAC→CAC)  cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
RA 664,088 C→A 9.6% P28P (CCG→CCT rlmH ← 23S rRNA m(3)Psi1915 pseudouridine methyltransferase, SAM‑dependent
RA 702,494 G→A 6.3% V316M (GTG→ATG)  glnS → glutamyl‑tRNA synthetase
RA 705,299 G→T 8.9% A18A (GCG→GCT chiQ → chitosugar‑induced verified lipoprotein
RA 725,164 G→A 6.2% G42G (GGG→GGA rhsC → Rhs family putative polymorphic toxin
RA 726,190 G→A 5.6% K384K (AAG→AAA rhsC → Rhs family putative polymorphic toxin
RA 726,848 A→G 5.4% T604A (ACC→GCC)  rhsC → Rhs family putative polymorphic toxin
RA 727,325 A→G 5.3% N763D (AAT→GAT) ‡ rhsC → Rhs family putative polymorphic toxin
RA 727,327 T→A 5.0% N763K (AAT→AAA) ‡ rhsC → Rhs family putative polymorphic toxin
RA 727,329 A→C 5.2% E764A (GAA→GCA)  rhsC → Rhs family putative polymorphic toxin
RA 727,369 A→G 7.9% A777A (GCA→GCG rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 10.7% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 728,467 T→C 7.7% I1143I (ATT→ATC rhsC → Rhs family putative polymorphic toxin
RA 733,915 A→G 5.6% K123R (AAG→AGG)  ybfD → H repeat‑associated putative transposase
RA 733,994 C→T 7.7% S149S (TCC→TCT ybfD → H repeat‑associated putative transposase
RA 816,707 G→T 9.7% A153A (GCG→GCT ybhM → UPF0005 family inner membrane protein
RA 868,982 G→A 14.3% G183D (GGC→GAC)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 968,986 G→T 6.7% intergenic (‑129/‑7) ycbC ← / → smtA DUF218 superfamily protein/putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 1,012,744 C→A 10.5% E338* (GAG→TAG)  ycbZ ← putative peptidase
RA 1,033,634 G→A 5.4% A147T (GCC→ACC)  cbdA → cytochrome bd‑II oxidase, subunit I
RA 1,045,764 C→G 20.0% D94E (GAC→GAG insB1 → IS1 transposase B
RA 1,045,767 G→C 24.7% V95V (GTG→GTC insB1 → IS1 transposase B
RA 1,045,771 A→G 16.1% I97V (ATA→GTA)  insB1 → IS1 transposase B
RA 1,045,775 G→A 16.0% W98* (TGG→TAG)  insB1 → IS1 transposase B
RA 1,045,806 C→A 12.8% S108S (TCC→TCA insB1 → IS1 transposase B
RA 1,045,830 A→T 8.2% V116V (GTA→GTT insB1 → IS1 transposase B
RA 1,062,181 G→T 7.2% A381S (GCG→TCG)  agp → glucose‑1‑phosphatase/inositol phosphatase
RA 1,068,155 G→A 14.3% T55T (ACC→ACT rutB ← ureidoacrylate amidohydrolase
RA 1,075,394 T→C 8.3% F212L (TTT→CTT)  putP → proline:sodium symporter
RA 1,195,844 A→G 6.9% L140P (CTT→CCT)  intE ← e14 prophage; putative integrase
RA 1,198,991 G→T 7.2% D90Y (GAC→TAC)  ymfL → e14 prophage; putative DNA‑binding transcriptional regulator
RA 1,219,339 G→A 5.5% pseudogene (213/237 nt) ycgI → pseudogene
RA 1,264,818 G→T 9.5% noncoding (40/67 nt) rdlA → sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA
RA 1,264,977 G→A 6.0% intergenic (+132/+182) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
RA 1,277,812 G→A 7.8% A831A (GCG→GCA narG → nitrate reductase 1, alpha subunit
RA 1,279,968 G→A 14.8% P303P (CCG→CCA narH → nitrate reductase 1, beta (Fe‑S) subunit
RA 1,282,249 A→G 12.6% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 7.0% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,454:1 +C 5.0% intergenic (+472/+68) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,494 T→G 5.7% intergenic (+512/+28) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,288,276 C→A 85.9% V35F (GTT→TTT)  hns ← global DNA‑binding transcriptional dual regulator H‑NS
RA 1,340,055 C→T 16.1% G315G (GGG→GGA gmr ← cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of Rnase II stability
RA 1,422,060 G→C 9.1% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 5.4% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,681 A→G 6.4% pseudogene (303/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,798 A→G 5.1% pseudogene (186/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,426,257 A→G 10.6% T984T (ACA→ACG stfR → Rac prophage; putative tail fiber protein
RA 1,432,843 C→T 6.5% G733G (GGG→GGA pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,461,113 A→G 6.2% pseudogene (1465/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,471,475 G→T 9.1% R692L (CGC→CTC)  ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,471,890 C→A 8.4% Q5K (CAG→AAG)  ynbA → inner membrane protein
RA 1,475,612 G→T 9.1% L149L (CTG→CTT ynbD → putative phosphatase inner membrane protein
RA 1,481,402 G→T 5.0% intergenic (+182/‑90) hrpA → / → ydcF putative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
RA 1,487,324 G→T 12.1% G200C (GGT→TGT)  trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,497,052 C→A 17.2% T113T (ACC→ACA ydcL → lipoprotein
RA 1,509,778 T→C 7.0% W254R (TGG→CGG)  ydcV → putative spermidine/putrescine transporter subunit
RA 1,523,601 G→A 8.6% pseudogene (1443/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,523,604 G→A 9.1% pseudogene (1446/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,527,189 A→G 7.2% T373A (ACG→GCG)  ydcC → H repeat‑associated putative transposase
RA 1,544,249 G→A 7.1% P864P (CCG→CCA fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,544,826 T→A 17.5% S37T (TCC→ACC)  fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,544,867 G→A 10.0% S50S (TCG→TCA fdnH → formate dehydrogenase‑N, Fe‑S (beta) subunit, nitrate‑inducible
RA 1,563,996 C→A 7.0% V251F (GTT→TTT)  gadC ← glutamate:gamma‑aminobutyric acid antiporter
RA 1,605,684 G→T 11.8% A143E (GCG→GAG)  yneF ← putative membrane‑bound diguanylate cyclase
RA 1,620,567 C→A 14.2% K357N (AAG→AAT dcp ← dipeptidyl carboxypeptidase II
RA 1,659,962 G→T 17.6% A131S (GCC→TCC)  clcB → H(+)/Cl(‑) exchange transporter
RA 1,665,324 C→T 6.9% intergenic (+115/‑309) ynfM → / → asr putative arabinose efflux transporter/acid shock‑inducible periplasmic protein
RA 1,681,729 G→A 6.3% R302R (CGC→CGT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,732 A→G 6.2% I301I (ATT→ATC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,885 C→A 5.2% A250A (GCG→GCT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,699,312 T→C 16.0% L36P (CTG→CCG)  cnu → nucleoid‑associated oriC‑binding protein; H‑NS and StpA stabilizing factor
RA 1,702,919 C→G 11.6% Q582E (CAA→GAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,158 A→T 10.5% R661R (CGA→CGT rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,173 A→G 9.5% K666K (AAA→AAG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,741,287 C→T 12.5% intergenic (+25/‑101) ydhR → / → ydhS putative monooxygenase/putative oxidoreductase
RA 1,846,485 T→A 5.1% N114K (AAT→AAA pncA → nicotinamidase/pyrazinamidase
RA 1,852,102 T→C 18.2% E330G (GAG→GGG)  ydjJ ← putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 1,866,529 G→T 6.3% E78* (GAA→TAA)  yeaJ → putative diguanylate cyclase
RA 1,879,041 G→T 6.5% E40D (GAG→GAT yeaW → putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster
RA 1,891,477 C→A 13.6% L97M (CTG→ATG)  sdaA → L‑serine dehydratase 1
RA 1,932,791 C→T 11.4% P296S (CCG→TCG)  pykA → pyruvate kinase II
RA 1,991,191 G→A 7.2% I35I (ATC→ATT yecC ← putative ABC superfamily transporter ATP‑binding subunit
RA 2,002,242 G→T 9.7% G162V (GGC→GTC)  yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein
RA 2,027,880 G→T 7.6% pseudogene (349/663 nt) yedS → pseudogene, outer membrane protein homology; putative outer membrane protein
RA 2,029,504 C→T 14.6% T63T (ACC→ACT hchA → glyoxalase III and Hsp31 molecular chaperone
RA 2,059,656 T→C 5.9% intergenic (‑21/+130) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,086,760 C→A 8.7% D294E (GAC→GAA hisC → histidinol‑phosphate aminotransferase
RA 2,141,742 G→T 6.4% A196A (GCG→GCT yegD → Hsp70 chaperone family protein
RA 2,150,813 G→C 8.7% W690C (TGG→TGC mdtB → multidrug efflux system, subunit B
RA 2,179,894 G→T 9.7% G167C (GGT→TGT)  rcnA → membrane protein conferring nickel and cobalt resistance
RA 2,215,032 G→T 6.1% A146E (GCG→GAG)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,271,518 C→T 11.8% D67N (GAC→AAC)  yejG ← uncharacterized protein
RA 2,298,340 T→G 21.8% intergenic (+468/+247) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,341 C→G 16.3% intergenic (+469/+246) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,355 G→A 12.5% intergenic (+483/+232) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,662 G→T 9.0% N189K (AAC→AAA ompC ← outer membrane porin protein C
RA 2,329,010 C→T 14.8% G372S (GGT→AGT)  yfaA ← DUF2138 family protein, putative host defense protein
RA 2,333,985 C→A 7.1% V1222L (GTG→TTG)  yfaL ← adhesin
RA 2,363,970 G→T 7.4% V158V (GTG→GTT arnD → undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
RA 2,369,611 G→A 5.1% R230C (CGC→TGC)  menB ← dihydroxynaphthoic acid synthetase
RA 2,397,608 G→T 8.5% P162H (CCT→CAT)  nuoB ← NADH:ubiquinone oxidoreductase, chain B
RA 2,425,649 G→A 6.4% A258V (GCC→GTC)  folC ← bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
RA 2,429,298 G→A 8.9% N277N (AAC→AAT usg ← putative semialdehyde dehydrogenase
RA 2,433,927 C→A 8.2% M386I (ATG→ATT fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,434,989 C→A 10.2% G32G (GGG→GGT fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,439,360 G→T 16.1% A168A (GCC→GCA mepA ← murein DD‑endopeptidase
RA 2,465,153 T→C 8.5% pseudogene (449/579 nt) yfdL ← pseudogene, CPS‑53 (KpLE1) prophage;Phage or Prophage Related
RA 2,488,470 G→T 14.0% intergenic (+18/+59) ypdI → / ← yfdY putative lipoprotein involved in colanic acid biosynthesis/DUF2545 family putative inner membrane protein
RA 2,547,258 C→T 6.7% Y225Y (TAC→TAT hemF → coproporphyrinogen III oxidase
RA 2,570,843 C→T 6.6% A298A (GCG→GCA maeB ← malic enzyme: putative oxidoreductase/putative phosphotransacetylase
RA 2,604,168 A→G 9.5% Y35C (TAC→TGC)  hyfI → hydrogenase 4, Fe‑S subunit
RA 2,640,133 C→A 7.4% S184S (TCG→TCT pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain
RA 2,655,826 T→C 11.0% intergenic (‑336/+116) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,663,322 C→A 7.9% P311H (CCC→CAC)  hcaE → 3‑phenylpropionate dioxygenase, large (alpha) subunit
RA 2,677,505 A→G 14.3% intergenic (+90/+108) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,677,506 C→T 14.3% intergenic (+91/+107) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,677,509 A→T 14.8% intergenic (+94/+104) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,677,510 G→T 14.9% intergenic (+95/+103) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,677,511 A→T 14.8% intergenic (+96/+102) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,677,512 T→A 12.1% intergenic (+97/+101) yphH → / ← glyA putative DNA‑binding transcriptional regulator/serine hydroxymethyltransferase
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,719,536 G→T 38.9% noncoding (12/120 nt) rrfG ← 5S ribosomal RNA of rrnG operon
RA 2,719,545 G→A 22.2% noncoding (3/120 nt) rrfG ← 5S ribosomal RNA of rrnG operon
RA 2,749,725 G→A 10.5% D70N (GAT→AAT)  intA → CP4‑57 prophage; integrase
RA 2,761,407 C→T 9.9% P113P (CCC→CCT yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,761,410 G→C 9.8% R114R (CGG→CGC yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,771,529 T→C 13.1% V1519V (GTA→GTG ypjA ← adhesin‑like autotransporter
RA 2,793,713 C→T 8.2% A70V (GCC→GTC)  ygaM → DUF883 family protein, putative membrane‑anchored ribosome‑binding protein
RA 2,804,924 C→T 7.3% R47C (CGT→TGT)  emrA → multidrug efflux system
RA 2,839,358 C→A 16.3% R158L (CGT→CTT)  hycE ← hydrogenase 3, large subunit
RA 2,845,707 G→T 10.5% V71V (GTG→GTT hypD → hydrogenase maturation protein
RA 2,903,090 Δ1 bp 6.3% intergenic (‑65/+163) pyrG ← / ← mazG CTP synthetase/nucleoside triphosphate pyrophosphohydrolase
RA 2,903,091 Δ1 bp 6.2% intergenic (‑66/+162) pyrG ← / ← mazG CTP synthetase/nucleoside triphosphate pyrophosphohydrolase
RA 2,903,092 Δ1 bp 6.2% intergenic (‑67/+161) pyrG ← / ← mazG CTP synthetase/nucleoside triphosphate pyrophosphohydrolase
RA 2,904,970 G→A 9.7% R681C (CGT→TGT)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,905,224 C→A 8.7% R596L (CGC→CTC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,908,741 A→G 12.0% Q109R (CAG→CGG)  barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 2,939,360 C→A 8.2% intergenic (‑159/+80) tcdA ← / ← mltA tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA/membrane‑bound lytic murein transglycosylase A
RA 3,000,170 G→T 5.6% E184* (GAA→TAA)  ygeW → putative carbamoyltransferase
RA 3,005,829 C→T 16.0% intergenic (+77/+144) yqeA → / ← yqeB putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
RA 3,005,830 G→A 16.0% intergenic (+78/+143) yqeA → / ← yqeB putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
RA 3,005,832 G→A 14.8% intergenic (+80/+141) yqeA → / ← yqeB putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
RA 3,064,718 T→G 16.5% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,072,806 A→G 100% intergenic (‑117/+197) cmtB ← / ← tktA putative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding
RA 3,088,680 C→A 15.8% T75K (ACA→AAA)  yggS → UPF0001 family protein, PLP‑binding
RA 3,092,899 G→A 10.0% T9M (ACG→ATG)  yggM ← DUF1202 family putative secreted protein
RA 3,095,806 G→T 5.5% R136S (CGC→AGC)  trmI ← tRNA m(7)G46 methyltransferase, SAM‑dependent
RA 3,096,855 A→T 7.1% N162Y (AAT→TAT)  mutY → adenine DNA glycosylase
RA 3,115,752 C→A 9.3% L471L (CTG→CTT glcB ← malate synthase G
RA 3,129,074 C→G 8.9% A219A (GCG→GCC) ‡ pitB ← phosphate transporter
RA 3,129,075 G→A 8.9% A219V (GCG→GTG) ‡ pitB ← phosphate transporter
RA 3,129,076 C→T 8.9% A219T (GCG→ACG) ‡ pitB ← phosphate transporter
RA 3,129,085 C→G 9.3% V216L (GTT→CTT)  pitB ← phosphate transporter
RA 3,129,086 A→G 9.0% I215I (ATT→ATC pitB ← phosphate transporter
RA 3,157,130 C→A 5.9% D735Y (GAT→TAT)  parC ← DNA topoisomerase IV, subunit A
RA 3,157,164 G→A 6.1% R723R (CGC→CGT parC ← DNA topoisomerase IV, subunit A
RA 3,210,552 C→T 23.5% R139C (CGC→TGC)  yqjI → PadR family putative transcriptional regulator
RA 3,211,302 A→G 10.4% R378R (CGT→CGC aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
RA 3,211,305 C→A 10.6% A377A (GCG→GCT aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
RA 3,213,138 G→T 7.4% V96L (GTG→TTG)  patA → putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
RA 3,240,194 G→A 10.3% V62I (GTC→ATC)  exuR → hexuronate regulon transcriptional repressor; autorepressor
RA 3,257,240 G→A 5.5% A379A (GCC→GCT tdcC ← L‑threonine/L‑serine transporter
RA 3,274,891 G→A 6.8% pseudogene (462/555 nt) agaA → pseudogene, N‑acetylgalactosamine‑6‑phosphate deacetylase fragment; putative N‑acetylgalactosamine‑6‑phosphate deacetylase
RA 3,408,537 A→G 5.5% Y49C (TAT→TGT)  acrF → multidrug efflux system protein
RA 3,416,890 T→G 11.0% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 17.4% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,435,515 C→A 11.8% A106S (GCA→TCA)  rpsM ← 30S ribosomal subunit protein S13
RA 3,446,514 G→T 7.4% P39Q (CCG→CAG)  rpsJ ← 30S ribosomal subunit protein S10
RA 3,464,590 G→A 5.0% T33T (ACC→ACT tufA ← translation elongation factor EF‑Tu 1
RA 3,473,059 G→A 7.3% A303V (GCG→GTG)  kefB ← potassium:proton antiporter
RA 3,536,567 C→A 8.2% F14L (TTC→TTA yhgA → transposase_31 family protein
RA 3,542,080 C→A 13.8% V450V (GTG→GTT malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,546,353 T→A 10.5% intergenic (‑521/‑91) malP ← / → malT maltodextrin phosphorylase/mal regulon transcriptional activator
RA 3,594,959 T→C 5.5% D163G (GAT→GGT)  ftsX ← inner membrane putative ABC superfamily transporter permease
RA 3,614,441 G→T 6.0% G630G (GGG→GGT rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,614,534 T→C 15.6% L661L (CTT→CTC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,615,572 G→A 10.0% R1007R (CGG→CGA rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,615,782 C→G 5.4% A1077A (GCC→GCG rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,616,061 T→C 5.0% Y1170Y (TAT→TAC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,616,121 G→C 6.4% R1190R (CGG→CGC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,618,160 A→G 6.9% G141G (GGA→GGG yhhI → putative transposase
RA 3,635,961 G→A 11.4% Q178* (CAG→TAG)  rsmJ ← 16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,636,203 G→T 5.7% A97E (GCA→GAA)  rsmJ ← 16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,641,201 C→T 8.5% intergenic (‑53/‑222) dinQ ← / → agrA UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/Inactive antisense sRNA
RA 3,642,356 G→T 9.5% G21V (GGT→GTT)  arsB → arsenite/antimonite transporter
RA 3,693,119 G→T 7.5% D516Y (GAC→TAC)  bcsG → DUF3260 family inner membrane protein associated with cellulose production
RA 3,695,359 C→A 7.7% P290P (CCG→CCT dppF ← dipeptide transporter
RA 3,717,571 C→T 9.3% E63K (GAA→AAA)  glyS ← glycine tRNA synthetase, beta subunit
RA 3,717,835 C→T 5.5% A282T (GCA→ACA)  glyQ ← glycine tRNA synthetase, alpha subunit
RA 3,747,137 G→A 8.2% A108V (GCG→GTG)  yiaV ← membrane fusion protein (MFP) component of efflux pump, signal anchor
RA 3,758,773 G→C 6.1% A1077A (GCG→GCC rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,758,971 C→T 6.0% I1143I (ATC→ATT rhsA → Rhs family protein, putative polymorphic toxin; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
RA 3,772,814 C→T 8.5% A134V (GCA→GTA)  lldR → dual role activator/repressor for lldPRD operon
RA 3,827,841 C→T 6.7% P19P (CCG→CCA yicI ← putative alpha‑glucosidase
RA 3,859,358 G→T 5.2% R92R (CGC→CGA yidE ← putative transporter
RA 3,868,004 G→T 5.1% Q173K (CAG→AAG)  dgoR ← putative DNA‑binding transcriptional regulator
RA 3,911,530 C→T 6.4% M32I (ATG→ATA atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit
RA 3,973,574:1 +G 10.5% coding (259/741 nt) wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,973,574:2 +G 10.5% coding (259/741 nt) wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,973,574:3 +C 10.5% coding (259/741 nt) wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,973,574:4 +A 10.5% coding (259/741 nt) wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,973,575:1 +C 10.5% coding (260/741 nt) wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 3,993,539 G→T 9.4% intergenic (+34/+113) uvrD → / ← yigE DNA‑dependent ATPase I and helicase II/DUF2233 family protein
RA 3,993,616 T→C 8.0% intergenic (+111/+36) uvrD → / ← yigE DNA‑dependent ATPase I and helicase II/DUF2233 family protein
RA 4,007,115 C→T 11.4% Q235* (CAG→TAG)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,034,757 C→A 21.3% A13S (GCC→TCC)  mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,076,606 T→G 14.3% G859G (GGA→GGC fdoG ← formate dehydrogenase‑O, large subunit
RA 4,076,623 A→T 11.8% F854I (TTT→ATT)  fdoG ← formate dehydrogenase‑O, large subunit
RA 4,077,245 C→A 11.8% A646A (GCG→GCT fdoG ← formate dehydrogenase‑O, large subunit
RA 4,148,281 G→T 7.8% noncoding (42/110 nt) oxyS ← sRNA antisense regulator activates genes that detoxify oxidative damage, Hfq‑dependent
RA 4,153,912 C→A 8.2% L116I (CTT→ATT)  btuB → vitamin B12/cobalamin outer membrane transporter
RA 4,165,449 C→T 27.2% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 27.2% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,173,148 A→G 7.3% Q659R (CAG→CGG)  rpoB → RNA polymerase, beta subunit
RA 4,173,745 G→A 10.2% G858D (GGT→GAT)  rpoB → RNA polymerase, beta subunit
RA 4,177,142 A→G 37.1% D622G (GAC→GGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,209 C→T 14.0% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,197,511 G→T 13.4% intergenic (‑51/‑564) purH ← / → rrsE fused IMP cyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase/16S ribosomal RNA of rrnE operon
RA 4,202,979 A→C 18.2% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,236,118 G→T 6.0% A77E (GCG→GAG)  malE ← maltose transporter subunit
RA 4,263,421 G→T 6.4% L127M (CTG→ATG)  uvrA ← ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
RA 4,288,032 G→A 6.2% S384S (AGC→AGT fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit
RA 4,312,921 C→A 18.9% pseudogene (127/426 nt) phnE ← defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
RA 4,315,805 G→T 13.0% intergenic (‑247/+411) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,905 T→G 6.3% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,315,955 Δ1 bp 6.1% intergenic (‑397/+261) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,353,271 G→T 11.5% P530Q (CCG→CAG)  dsbD ← fused thiol:disulfide interchange protein: activator of DsbC/conserved protein
RA 4,451,424:1 +A 5.4% coding (1054/2139 nt) nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,455,722 C→A 13.7% Q92H (CAG→CAT treB ← fused trehalose(maltose)‑specific PTS enzyme: IIB component/IIC component
RA 4,463,488 A→G 6.9% L128S (TTA→TCA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,472,667 C→A 9.8% A330S (GCA→TCA)  valS ← valyl‑tRNA synthetase
RA 4,498,909 G→T 5.2% pseudogene (84/785 nt) insO → pseudogene, IS911 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,499,846 C→A 7.0% pseudogene (226/330 nt) yjhV → pseudogene, KpLE2 phage‑like element
RA 4,508,633 A→G 6.1% pseudogene (96/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,508,651 T→C 8.1% pseudogene (114/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,516,029 A→G 7.0% L226P (CTA→CCA)  sgcR ← putative DNA‑binding transcriptional regulator
RA 4,521,810 A→G 10.8% intergenic (‑342/+57) sgcX ← / ← yjhY putative endoglucanase with Zn‑dependent exopeptidase domain/pseudogene, zinc finger protein family
RA 4,550,326 G→T 6.9% L58M (CTG→ATG)  yjiH ← nucleoside recognition pore and gate family putative inner membrane transporter
RA 4,551,778 C→A 7.2% D239Y (GAT→TAT)  yjiJ ← DUF1228 family putative inner membrane MFS superfamily transporter
RA 4,561,269 G→A 80.9% S41S (AGC→AGT yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase
RA 4,612,009 G→T 9.6% V142L (GTG→TTG)  yjjJ → putative protein kinase
RA 4,619,818 G→A 14.3% C174C (TGC→TGT yjjK ← putative transporter subunit of ABC superfamily: ATP‑binding component
RA 4,626,934 C→A 8.3% A141D (GCC→GAC)  creC → sensory histidine kinase in two‑component regulatory system with CreB or PhoB, regulator of the CreBC regulon

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 2027120 2027506 387 6 [4] [4] 5 rseX rseX
* * ÷ CP009273 3448799 3448865 67 7 [4] [3] 28 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ CP009273 4323132 4323683 552 6 [4] [4] 5 basR response regulator in two‑component regulatory system with BasS

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 311135NA (NA)3 (0.070) 3/244 NT NA coding (134/300 nt) insE1 IS3 transposase A
?CP009273 311327 = NA (NA)coding (30/867 nt) insF1 IS3 transposase B
* ? CP009273 360104 =29 (0.620)33 (0.710) 16/248 NT 53.2% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =18 (0.390)34 (0.840) 15/216 NT 76.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 5 (0.120)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326114 (0.300)5 (0.120) 4/216 NT 36.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 5 (0.120)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related