breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 56,417 C→T 5.3% P231P (CCG→CCA lptD ← LPS assembly OM complex LptDE, beta‑barrel component
RA 97,310 G→T 6.2% R20L (CGG→CTG)  murC → UDP‑N‑acetylmuramate:L‑alanine ligase
RA 221,407 A→T 5.2% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 5.8% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 6.0% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 9.8% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 10.2% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 239,075 G→T 5.7% A239E (GCA→GAA)  fadE ← acyl coenzyme A dehydrogenase
RA 286,409 T→A 6.8% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 293,469 C→A 5.2% pseudogene (34/78 nt) ykgT ← pseudogene, MobA‑related
RA 345,428 T→C 6.7% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 347,281 G→T 5.7% G119V (GGC→GTC)  prpD → 2‑methylcitrate dehydratase
RA 353,317 C→T 5.3% intergenic (+152/+185) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 361,680 G→T 5.5% R22S (CGC→AGC)  lacI ← DNA‑binding transcriptional repressor
RA 365,405 C→A 8.6% P299Q (CCG→CAG)  mhpB → 2,3‑dihydroxyphenylpropionate 1,2‑dioxygenase
RA 369,637 G→T 5.6% R105L (CGG→CTG)  mhpE → 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I
RA 370,501 G→A 5.5% intergenic (+164/‑414) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 393,552 C→A 6.3% N75K (AAC→AAA yaiW → putative lipoprotein
RA 406,581 Δ1 bp 6.0% intergenic (+73/+172) mak → / ← araJ manno(fructo)kinase/arabinose‑inducible putative transporter, MFS family
RA 418,995 T→G 13.7% L341R (CTG→CGG)  malZ → maltodextrin glucosidase
RA 433,686 C→A 5.8% intergenic (‑95/+85) yajO ← / ← dxs 2‑carboxybenzaldehyde reductase/1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
RA 444,190 G→T 6.1% Y636* (TAC→TAA cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 520,636 G→A 5.8% E640E (GAG→GAA rhsD → Rhs family putative polymorphic toxin
RA 529,223 C→A 5.4% L253M (CTG→ATG)  allR → transcriptional repressor of all and gcl operons; glyoxylate‑induced
RA 690,087 G→T 5.6% H109N (CAC→AAC)  miaB ← tRNA‑i(6)A37 methylthiotransferase
RA 697,697 G→T 6.6% L171M (CTG→ATG)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 725,098 C→T 5.7% V20V (GTC→GTT rhsC → Rhs family putative polymorphic toxin
RA 726,319 G→C 5.5% A427A (GCG→GCC rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 5.6% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 733,372 T→C 6.2% pseudogene (1092/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
RA 845,088 C→A 5.4% A156S (GCC→TCC)  rhtA ← threonine and homoserine efflux system
RA 976,236 G→T 5.3% A1485A (GCG→GCT mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 1,012,469 C→T 7.3% Q429Q (CAG→CAA ycbZ ← putative peptidase
RA 1,081,867 C→A 6.5% A37S (GCC→TCC)  pgaD ← biofilm PGA synthase PgaCD, regulatory subunit; c‑di‑GMP‑stimulated activity and dimerization
RA 1,264,818 G→T 5.3% noncoding (40/67 nt) rdlA → sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA
RA 1,265,875 T→C 5.4% noncoding (27/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,282,421 A→G 5.1% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,430 C→T 6.1% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,439 A→G 6.9% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,292,010 C→A 10.6% S523I (AGC→ATC)  adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
RA 1,301,639 G→T 6.4% L422I (CTT→ATT)  clsA ← cardiolipin synthase 1
RA 1,343,387 C→A 5.4% L326L (CTG→CTT yciW ← putative oxidoreductase
RA 1,418,604 G→T 5.1% W189L (TGG→TTG)  trkG → Rac prophage; potassium transporter subunit
RA 1,422,040 A→G 13.8% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 16.6% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,460,752 C→T 5.0% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,861 G→A 8.3% pseudogene (1213/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,887 G→C 7.2% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,896 C→T 7.0% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,461,046 C→T 5.7% pseudogene (1398/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,471,744 G→T 6.8% intergenic (+37/‑134) ydbD → / → ynbA PF10971 family putative periplasmic methylglyoxal resistance protein/inner membrane protein
RA 1,492,200 G→T 5.7% W363L (TGG→TTG)  opgD → osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
RA 1,499,367 G→T 5.3% A324E (GCA→GAA)  ydcO ← BenE family inner membrane putative transporter
RA 1,522,209 C→T 7.1% pseudogene (51/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,527,173 C→T 5.6% Y367Y (TAC→TAT ydcC → H repeat‑associated putative transposase
RA 1,529,167 G→T 5.2% intergenic (+41/+55) nhoA → / ← yddE N‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein
RA 1,542,239 C→T 5.4% R194R (CGC→CGT fdnG → formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
RA 1,629,823 A→G 6.2% G94G (GGT→GGC stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,652,056 C→A 5.6% R79S (CGC→AGC)  ynfD → DUF1161 family periplasmic protein
RA 1,673,954 C→T 5.1% G47G (GGC→GGT ydgI → putative arginine/ornithine antiporter transporter
RA 1,681,696 A→G 5.7% G313G (GGT→GGC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,729 G→A 7.7% R302R (CGC→CGT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,732 A→G 6.6% I301I (ATT→ATC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,735 C→A 7.7% V300V (GTG→GTT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,681,741 G→A 6.2% I298I (ATC→ATT fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,715,530 A→T 7.3% K4* (AAA→TAA)  ydhJ → putative membrane fusion protein (MFP) of YdhJK efflux pump
RA 1,760,360 C→T 5.3% M861I (ATG→ATA ydiJ ← putative FAD‑linked oxidoreductase
RA 1,783,404 C→A 5.5% P238Q (CCG→CAG)  aroH → 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible
RA 1,800,810 G→T 6.2% G62C (GGT→TGT)  pfkB → 6‑phosphofructokinase II
RA 1,967,228:1 +T 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 1,967,228:2 +T 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 1,967,228:3 +G 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 1,967,228:4 +C 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 1,967,228:5 +T 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 1,967,228:6 +C 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 1,967,228:7 +A 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 1,967,228:8 +C 5.1% coding (369/504 nt) cheW ← purine‑binding chemotaxis protein
RA 2,012,632 C→A 5.1% Q256K (CAA→AAA)  fliK → flagellar hook‑length control protein
RA 2,014,492 C→A 5.1% L309I (CTC→ATC)  fliM → flagellar motor switching and energizing component
RA 2,027,199 G→A 5.4% noncoding (70/91 nt) rseX → sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent
RA 2,059,656 T→C 5.1% intergenic (‑21/+130) yeeH ← / ← insH1 pseudogene/IS5 transposase and trans‑activator
RA 2,088,305 G→A 5.3% G97D (GGC→GAC)  hisH → imidazole glycerol phosphate synthase, glutamine amidotransferase subunit with HisF
RA 2,142,326 T→C 11.9% L391P (CTG→CCG)  yegD → Hsp70 chaperone family protein
RA 2,170,401 G→T 5.1% R95S (CGT→AGT)  gatY ← D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit
RA 2,174,270 G→T 7.2% E233* (GAA→TAA)  yegU → ADP‑ribosylglycohydrolase family protein
RA 2,184,239 G→T 5.4% P51T (CCC→ACC)  yehB ← putative outer membrane protein
RA 2,370,515 G→A 7.3% A186V (GCC→GTC)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,531,960 C→G 5.8% intergenic (+118/+71) yfeS → / ← cysM WGR domain protein/cysteine synthase B (O‑acetylserine sulfhydrolase B)
RA 2,553,008 A→T 7.5% T264T (ACA→ACT intZ → CPZ‑55 prophage; putative phage integrase
RA 2,564,235 C→T 6.8% Q292Q (CAG→CAA eutE ← aldehyde oxidoreductase, ethanolamine utilization protein
RA 2,577,997 G→T 6.0% G296G (GGC→GGA aegA ← putative oxidoreductase, FeS binding subunit/NAD/FAD‑binding subunit
RA 2,663,836 C→A 5.5% R30S (CGT→AGT)  hcaF → 3‑phenylpropionate dioxygenase, small (beta) subunit
RA 2,670,551 C→A 7.1% R390R (CGG→CGT yphE ← putative sugar transporter subunit of ABC superfamily, ATP‑binding component
RA 2,719,536 G→T 23.1% noncoding (12/120 nt) rrfG ← 5S ribosomal RNA of rrnG operon
RA 2,771,529 T→C 6.1% V1519V (GTA→GTG ypjA ← adhesin‑like autotransporter
RA 2,771,561 T→C 5.8% S1509G (AGT→GGT)  ypjA ← adhesin‑like autotransporter
RA 2,782,622 T→C 8.0% G93G (GGT→GGC csiD → carbon starvation protein
RA 2,789,625 C→A 10.2% A198D (GCC→GAC)  csiR → transcriptional repressor of csiD
RA 2,811,350 A→C 7.1% intergenic (‑131/+68) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,811,356 A→G 7.7% intergenic (‑137/+62) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,811,405 A→G 7.6% intergenic (‑186/+13) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,811,408 T→A 6.6% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,833,065 C→A 7.3% N61K (AAC→AAA ascF → fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
RA 2,881,708 C→A 5.7% intergenic (‑37/+38) cysH ← / ← cysI phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent/sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
RA 2,881,714 Δ1 bp 7.4% intergenic (‑43/+32) cysH ← / ← cysI phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent/sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
RA 2,881,723 G→T 8.0% intergenic (‑52/+23) cysH ← / ← cysI phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent/sulfite reductase, beta subunit, NAD(P)‑binding, heme‑binding
RA 3,048,255 C→G 8.5% A183A (GCG→GCC ygfB ← UPF0149 family protein
RA 3,064,718 T→G 16.3% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,353,283 C→A 6.0% Q243K (CAG→AAG)  gltD → glutamate synthase, 4Fe‑4S protein, small subunit
RA 3,354,909 G→T 5.8% K125N (AAG→AAT gltF → periplasmic protein
RA 3,374,414 C→T 5.4% L105L (CTG→TTG)  degQ → serine endoprotease, periplasmic
RA 3,386,478 G→T 6.1% P1047Q (CCG→CAG)  yhdP ← DUF3971‑AsmA2 domains protein
RA 3,416,899 A→G 11.7% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,422,276 Δ1 bp 6.2% intergenic (‑155/‑319) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,422,282 Δ1 bp 5.8% intergenic (‑161/‑313) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,422,286 T→G 6.5% intergenic (‑165/‑309) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,470,094 C→A 6.1% G229C (GGT→TGT)  fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
RA 3,607,325 C→A 6.3% P100Q (CCG→CAG)  nikA → nickel‑binding, heme‑binding periplasmic protein
RA 3,615,572 G→A 5.6% R1007R (CGG→CGA rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,618,160 A→G 7.3% G141G (GGA→GGG yhhI → putative transposase
RA 3,753,389 C→A 5.5% A407A (GCG→GCT selA ← selenocysteine synthase
RA 3,766,819 G→T 7.6% M393I (ATG→ATT mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
RA 3,780,173 C→A 5.2% intergenic (+9/‑25) gpmM → / → envC phosphoglycero mutase III, cofactor‑independent/activator of AmiB,C murein hydrolases, septal ring factor
RA 3,813,894 C→A 6.6% D213Y (GAC→TAC)  ligB ← DNA ligase, NAD(+)‑dependent
RA 3,822,340 A→C 28.6% Q12H (CAA→CAC xanP → xanthine permease
RA 3,901,867 T→G 7.7% intergenic (‑141/+42) pstB ← / ← pstA phosphate transporter subunit/phosphate transporter subunit
RA 3,904,600 G→T 7.1% Q96K (CAG→AAG)  pstS ← periplasmic phosphate binding protein, high‑affinity
RA 3,935,371 G→T 5.6% noncoding (204/1542 nt) rrsC → 16S ribosomal RNA of rrnC operon
RA 3,971,399 A→G 7.0% N172S (AAT→AGT)  wecF → TDP‑Fuc4NAc:lipidIIFuc4NAc transferase
RA 4,132,070 C→A 6.0% C27F (TGT→TTT)  frwA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
RA 4,164,335 C→A 5.9% G207V (GGG→GTG)  coaA ← pantothenate kinase
RA 4,165,449 C→T 12.4% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 12.4% noncoding (51/85 nt) tyrU → tRNA‑Tyr
JC 4,178,733 +CCGGCAATC 83.3% coding (3456/4224 nt) rpoC → RNA polymerase, beta prime subunit
RA 4,233,133 G→T 5.4% T104T (ACC→ACA malG ← maltose transporter subunit
RA 4,336,238 A→G 9.4% R302R (CGT→CGC fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,241 G→A 9.7% I301I (ATC→ATT fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,244 A→C 8.1% V300V (GTT→GTG fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,336,250 A→G 7.8% I298I (ATT→ATC fumB ← anaerobic class I fumarate hydratase (fumarase B)
RA 4,575,190 C→T 6.2% pseudogene (1443/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,604,397 G→A 6.8% intergenic (+320/+100) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,623,921 G→T 5.0% W103L (TGG→TTG)  ytjC → phosphatase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 80475 80671 197 31 [23] [21] 34 leuL/leuO leu operon leader peptide/global transcription factor
* * ÷ CP009273 4249411 4249970 560 30 [24] [25] 29 zur zur

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 353228NA (NA)8 (0.080) 3/238 NT NA noncoding (34/79 nt) REP27 (repetitive extragenic palindromic) element; contains 2 REP sequences REP27 (repetitive extragenic palindromic) element; contains 2 REP sequences
?CP009273 736444 = NA (NA)noncoding (35/106 nt) RIP66 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP66 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? CP009273 360104 =93 (0.870)114 (1.070) 41/250 NT 55.1% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 370386 =NA (NA)10 (0.140) 4/164 NT 8.2% noncoding (1/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372978 = 124 (1.600)intergenic (+211/+13) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 370704 =145 (1.360)18 (0.200) 6/212 NT 11.1% noncoding (319/436 nt) REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences REP31 (repetitive extragenic palindromic) element; contains 9 REP sequences
?CP009273 372963 = 167 (1.850)noncoding (173/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 1203246 =47 (0.440)43 (0.460) 21/218 NT 50.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 42 (0.450)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326151 (0.480)25 (0.270) 12/218 NT 36.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 42 (0.450)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 2283592NA (NA)125 (1.190) 47/246 NT 94.7% noncoding (1/1195 nt) IS5 repeat region
?CP009273 2399636 = 7 (0.070)coding (485/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
* ? CP009273 = 23996397 (0.070)89 (0.830) 39/250 NT 92.7% coding (482/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
?CP009273 = 3124699 NA (NA)intergenic (+146/+1) insH1/yghQ IS5 transposase and trans‑activator/putative inner membrane polysaccharide flippase