breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 7,675 C→A 8.0% M95I (ATG→ATT yaaJ ← putative transporter
RA 18,839 G→T 6.6% R42L (CGA→CTA)  nhaR → transcriptional activator of nhaA
RA 19,702 C→A 8.3% intergenic (+82/+109) nhaR → / ← insB1 transcriptional activator of nhaA/IS1 transposase B
RA 23,573 C→A 8.3% H395N (CAC→AAC)  ileS → isoleucyl‑tRNA synthetase
RA 52,948 C→A 6.8% E157* (GAA→TAA)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
RA 73,068 G→T 11.5% P240Q (CCG→CAG)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 75,293 G→T 9.2% intergenic (+7/+42) setA → / ← leuD broad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit
RA 114,678 G→A 10.8% T152M (ACG→ATG)  nadC ← quinolinate phosphoribosyltransferase
RA 183,678 G→T 6.8% T487K (ACG→AAG)  glnD ← uridylyltransferase
RA 197,763 C→A 6.0% P102P (CCC→CCA lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
RA 199,564 G→T 8.2% G173V (GGC→GTC)  lpxA → UDP‑N‑acetylglucosamine acetyltransferase
RA 221,425 T→C 7.1% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 7.3% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 235,845 G→A 6.2% pseudogene (253/273 nt) yafF → pseudogene, H repeat‑associated protein
RA 241,797 G→T 22.2% Q166K (CAG→AAG)  yafK ← L,D‑transpeptidase‑related protein
RA 242,788 C→A 7.4% G68C (GGC→TGC)  dinJ ← antitoxin of YafQ‑DinJ toxin‑antitoxin system
RA 259,857 G→T 5.6% S34R (AGC→AGA ykfH ← uncharacterized protein
RA 263,285 G→A 14.0% D144D (GAC→GAT yafZ ← CP4‑6 prophage; conserved protein
RA 263,324 A→G 8.3% G131G (GGT→GGC yafZ ← CP4‑6 prophage; conserved protein
RA 263,333 G→C 12.6% L128L (CTC→CTG yafZ ← CP4‑6 prophage; conserved protein
RA 263,336 C→A 6.9% G127G (GGG→GGT yafZ ← CP4‑6 prophage; conserved protein
RA 273,623 G→T 6.2% V297V (GTC→GTA afuC ← CP4‑6 prophage; putative ferric transporter subunit
RA 314,868 G→T 6.4% T282N (ACC→AAC)  rclA ← reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 331,526 C→A 6.5% T179K (ACG→AAG)  yahD → ankyrin repeat protein
RA 339,480 G→T 10.7% G296C (GGC→TGC)  yahK → aldehyde reductase, NADPH‑dependent, Zn‑containing, broad specificity
RA 362,046 C→T 6.0% G203D (GGC→GAC)  mhpR ← mhp operon transcriptional activator
RA 370,617 C→T 7.6% intergenic (+280/‑298) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 391,249 C→A 6.8% W165C (TGG→TGT ampH ← D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase
RA 422,610 C→A 6.9% R340S (CGT→AGT)  tgt → tRNA‑guanine transglycosylase
RA 429,111 C→A 6.9% T67T (ACC→ACA ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
RA 437,433 G→T 10.8% L143L (CTG→CTT thiI → tRNA s(4)U8 sulfurtransferase
RA 456,176 G→T 8.3% L611L (CTG→CTT lon → DNA‑binding ATP‑dependent protease La
RA 464,698 C→A 14.0% V124V (GTC→GTA mdlA → putative multidrug transporter subunit of ABC superfamily, ATP‑binding component
RA 469,378 G→A 5.2% W319* (TGG→TGA amtB → ammonium transporter
RA 479,223 G→A 9.6% Q213* (CAG→TAG)  acrB ← multidrug efflux system protein
RA 498,346 C→A 6.3% G117C (GGT→TGT)  ybaL ← inner membrane putative NAD(P)‑binding transporter
RA 505,648 G→A 7.5% D396D (GAC→GAT copA ← copper transporter
RA 510,558 C→T 6.9% A224A (GCG→GCA qmcA ← PHB domain membrane‑anchored putative protease
RA 512,594 Δ1 bp 7.5% coding (556/780 nt) fetB → iron exporter permease subunit, ABC transporter FetAB; peroxide resistance protein
RA 512,595 Δ1 bp 7.5% coding (557/780 nt) fetB → iron exporter permease subunit, ABC transporter FetAB; peroxide resistance protein
RA 518,784 C→A 6.1% S23* (TCG→TAG)  rhsD → Rhs family putative polymorphic toxin
RA 557,647 G→T 6.3% W832L (TGG→TTG)  sfmD → putative outer membrane export usher protein
RA 582,258 G→A 5.9% A36V (GCC→GTC)  envY ← porin thermoregulatory transcriptional activator
RA 593,276 C→A 12.0% V114V (GTC→GTA cusB → copper/silver efflux system, membrane fusion protein
RA 638,051 G→T 5.6% D170Y (GAT→TAT)  ybdR → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 660,171 G→T 6.9% S159* (TCA→TAA)  rlpA ← septal ring protein, suppressor of prc, minor lipoprotein
RA 691,051 C→T 6.4% D165D (GAC→GAT ubiF → 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase
RA 725,992 C→A 7.6% P318P (CCC→CCA rhsC → Rhs family putative polymorphic toxin
RA 726,169 T→C 5.4% S377S (AGT→AGC rhsC → Rhs family putative polymorphic toxin
RA 726,190 G→A 8.2% K384K (AAG→AAA rhsC → Rhs family putative polymorphic toxin
RA 726,513 G→T 5.8% W492L (TGG→TTG)  rhsC → Rhs family putative polymorphic toxin
RA 726,667 T→C 6.6% T543T (ACT→ACC rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 9.5% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 728,467 T→C 5.4% I1143I (ATT→ATC rhsC → Rhs family putative polymorphic toxin
RA 728,536 G→T 5.4% W1166C (TGG→TGT rhsC → Rhs family putative polymorphic toxin
RA 729,930 A→G 9.1% pseudogene (342/1521 nt) rhsO → pseudogene, Rhs family protein
RA 730,138 A→G 7.3% pseudogene (550/1521 nt) rhsO → pseudogene, Rhs family protein
RA 735,551 G→A 8.9% D197N (GAT→AAT)  phr → deoxyribodipyrimidine photolyase, FAD‑binding
RA 745,950 C→A 5.8% G559V (GGT→GTT)  ybgQ ← putative outer membrane protein
RA 768,819 T→A 6.2% S108T (TCC→ACC)  cydB → cytochrome d terminal oxidase, subunit II
RA 788,996:1 +C 7.8% coding (249/1473 nt) modF ← fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
RA 788,996:2 +G 7.8% coding (249/1473 nt) modF ← fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
RA 788,996:3 +A 7.8% coding (249/1473 nt) modF ← fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
RA 840,848 G→T 5.3% T30K (ACG→AAG)  ybiO ← mechanosensitive channel protein, intermediate conductance
RA 908,132 Δ1 bp 7.8% coding (1139/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,133 Δ1 bp 7.8% coding (1138/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,134 Δ1 bp 7.8% coding (1137/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,135 Δ1 bp 7.5% coding (1136/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,136 Δ1 bp 7.5% coding (1135/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,137 Δ1 bp 7.5% coding (1134/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,138 Δ1 bp 7.5% coding (1133/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,139 Δ1 bp 7.5% coding (1132/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 908,140 Δ1 bp 7.5% coding (1131/1653 nt) hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 910,499 C→T 7.3% intergenic (‑186/+309) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 919,348 C→A 7.8% P210Q (CCG→CAG)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 919,905 G→T 5.5% D396Y (GAT→TAT)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 923,065 G→T 8.6% V529V (GTC→GTA cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 940,395 G→T 9.8% Q207K (CAG→AAG)  ycaC ← putative isochorismatase family hydrolase
RA 990,493 C→A 10.9% E318* (GAA→TAA)  ssuD ← alkanesulfonate monooxygenase, FMNH(2)‑dependent
RA 1,000,299 T→C 9.5% L26L (TTA→CTA)  pyrD → dihydro‑orotate oxidase, FMN‑linked
RA 1,045,283 T→G 8.2% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
RA 1,069,762 C→T 5.5% A22V (GCG→GTG)  rutR → rut operon transcriptional repressor for
RA 1,071,713 G→T 9.5% R876S (CGC→AGC)  putA ← fused DNA‑binding transcriptional regulator/proline dehydrogenase/pyrroline‑5‑carboxylate dehydrogenase
RA 1,191,425 G→T 8.3% D283Y (GAT→TAT)  icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,198,894 G→T 10.0% P57P (CCG→CCT ymfL → e14 prophage; putative DNA‑binding transcriptional regulator
RA 1,203,113 G→T 6.2% A53S (GCA→TCA)  stfP → e14 prophage; uncharacterized protein
RA 1,218,996 G→A 8.6% intergenic (+91/‑24) ymgJ → / → ycgI uncharacterized protein/pseudogene
RA 1,264,818 G→T 5.5% noncoding (40/67 nt) rdlA → sRNA antisense regulator affects LdrA translation; proposed addiction module in LDR‑A repeat, with toxic peptide LdrA
RA 1,265,182 C→T 7.0% G29S (GGC→AGC)  ldrB ← toxic polypeptide, small
RA 1,266,575 G→T 8.0% S244* (TCG→TAG)  chaA ← calcium/sodium:proton antiporter
RA 1,274,639 C→A 8.3% F409L (TTC→TTA narK → nitrate/nitrite transporter
RA 1,276,027 T→C 6.4% T236T (ACT→ACC narG → nitrate reductase 1, alpha subunit
RA 1,282,163 C→A 6.3% intergenic (+181/+359) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,249 A→G 13.4% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,421 A→G 7.9% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,608 T→C 5.9% noncoding (85/171 nt)
T9A (ACT→GCT) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,294,341 C→T 5.9% S96S (AGC→AGT ychE → UPF0056 family inner membrane protein
RA 1,337,028 G→T 7.2% Q38H (CAG→CAT yciH → initiation factor function partial mimic, SUI1 family
RA 1,364,188 C→T 9.1% S81S (AGC→AGT pspE → thiosulfate:cyanide sulfurtransferase (rhodanese)
RA 1,395,455 C→T 6.2% S192S (TCG→TCA abgT ← p‑aminobenzoyl‑glutamate transporter; membrane protein
RA 1,399,985 C→T 7.5% noncoding (24/96 nt) mcaS ← motility and biofilm regulator sRNA
RA 1,422,060 G→C 13.2% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,252 A→G 8.4% pseudogene (732/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 5.9% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,460,602 T→C 8.9% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,461,113 A→G 5.0% pseudogene (1465/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,479,676 G→T 8.7% D787Y (GAT→TAT)  hrpA → putative ATP‑dependent helicase
RA 1,516,296 G→A 6.0% R343C (CGT→TGT)  yncD ← putative iron outer membrane transporter
RA 1,599,259 A→G 7.8% E319G (GAG→GGG)  lsrD → autoinducer 2 import system permease protein
RA 1,626,911 T→C 5.5% intergenic (‑369/‑418) ydfJ ← / → ydfK pseudogene, MFS transporter family; interrupted by Qin prophage;Phage or Prophage Related; putative transport protein/cold shock protein, function unknown, Qin prophage
RA 1,652,338 G→T 10.2% E5* (GAA→TAA)  ynfE → putative selenate reductase, periplasmic
RA 1,681,696 A→G 13.9% G313G (GGT→GGC fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,703,101 C→G 7.0% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,745,991 A→G 6.0% P699P (CCT→CCC ydhV ← putative oxidoreductase subunit
RA 1,749,213 G→T 13.9% T62T (ACC→ACA ydhZ ← uncharacterized protein
RA 1,754,361 C→T 9.5% E140E (GAG→GAA sufS ← cysteine desulfurase, stimulated by SufE; selenocysteine lyase, PLP‑dependent
RA 1,759,841 C→A 6.7% E17* (GAA→TAA)  menI ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,793,720 G→T 5.1% A96D (GCT→GAT)  rplT ← 50S ribosomal subunit protein L20
RA 1,805,250 T→C 7.2% A20A (GCT→GCC ydjN → putative transporter
RA 1,813,772 G→T 8.9% S97S (TCC→TCA chbA ← N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
RA 1,874,940 G→T 7.1% P26Q (CCA→CAA)  leuE ← leucine efflux protein
RA 1,876,540 T→C 6.0% P124P (CCT→CCC dmlA → D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase
RA 1,886,286 C→A 7.7% D403Y (GAC→TAC)  yoaA ← putative ATP‑dependent helicase, DinG family
RA 1,912,442 G→A 10.0% G226S (GGC→AGC)  yebT → MCE domain protein
RA 1,917,040 G→A 16.0% R63C (CGT→TGT)  pphA ← serine/threonine‑specific protein phosphatase 1
RA 1,965,072 C→A 11.5% R57L (CGG→CTG)  tap ← methyl‑accepting protein IV
RA 1,987,217 C→A 11.6% S324I (AGC→ATC)  uvrC ← excinuclease UvrABC, endonuclease subunit
RA 2,002,678 C→T 5.3% G307G (GGC→GGT yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein
RA 2,032,505 C→A 8.6% A23A (GCC→GCA hiuH → hydroxyisourate hydrolase
RA 2,040,512 T→C 12.1% Y698Y (TAT→TAC yeeJ → putative adhesin
RA 2,070,102 C→T 6.3% T105I (ACA→ATA)  yeeS → CP4‑44 prophage; putative DNA repair protein
RA 2,072,388 G→T 13.3% pseudogene (162/186 nt) yoeF → pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
RA 2,084,978 G→A 5.9% G134D (GGC→GAC)  hisD → bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
RA 2,101,241 C→T 7.0% P190P (CCG→CCA glf ← UDP‑galactopyranose mutase, FAD/NAD(P)‑binding
RA 2,108,810 G→T 13.4% Q190K (CAA→AAA)  wcaM ← colanic acid biosynthesis protein
RA 2,134,148 G→T 10.8% Q316K (CAA→AAA)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,134,628 C→T 12.1% V156M (GTG→ATG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,141,783 G→T 7.7% R210L (CGG→CTG)  yegD → Hsp70 chaperone family protein
RA 2,150,699 G→T 12.5% P652P (CCG→CCT mdtB → multidrug efflux system, subunit B
RA 2,156,618 G→T 7.8% D88Y (GAT→TAT)  baeS → sensory histidine kinase in two‑component regulatory system with BaeR
RA 2,172,672 C→A 13.1% R125S (CGT→AGT)  yegT → nucleoside transporter, low affinity
RA 2,177,322 C→A 6.7% R225L (CGC→CTC)  thiD ← bifunctional hydroxy‑methylpyrimidine kinase/ hydroxy‑phosphomethylpyrimidine kinase
RA 2,193,595 C→T 5.1% pseudogene (795/948 nt) yehH → DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
RA 2,211,227 C→T 10.2% L270L (CTG→CTA yehY ← inner membrane putative ABC superfamily transporter permease
RA 2,259,339 C→A 6.5% I137I (ATC→ATA yeiP → elongation factor P‑like protein
RA 2,262,159 G→T 9.3% Q284H (CAG→CAT yeiR → Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
RA 2,267,115 G→A 10.5% A425T (GCC→ACC)  yejA → microcin C transporter YejABEF, periplasmic binding protein; ABC family
RA 2,286,673 C→T 16.3% G571R (GGG→AGG)  ccmF ← heme lyase, CcmF subunit
RA 2,293,669 C→A 6.1% G24V (GGG→GTG)  napG ← ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)
RA 2,306,106 C→T 7.5% L41L (CTG→CTA ompC ← outer membrane porin protein C
RA 2,309,584 C→T 9.5% P873L (CCA→CTA)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,364,782 C→A 8.9% A132A (GCC→GCA arnT → 4‑amino‑4‑deoxy‑L‑arabinose transferase
RA 2,377,742 C→A 6.5% R487L (CGC→CTC)  yfbK ← Von Willebrand factor domain putative lipoprotein
RA 2,401,472 C→A 5.8% H145N (CAT→AAT)  alaA → valine‑pyruvate aminotransferase 2
RA 2,511,819 G→T 13.4% intergenic (‑109/‑127) alaW ← / → yfeC tRNA‑Ala/DUF1323 family putative DNA‑binding protein
RA 2,517,181 G→A 5.4% P55L (CCT→CTT)  xapB ← xanthosine transporter
RA 2,537,076 Δ1 bp 9.6% intergenic (‑189/+115) cysP ← / ← ucpA thiosulfate‑binding protein/furfural resistance protein, putative short‑chain oxidoreductase
RA 2,573,244 G→T 6.2% E84* (GAA→TAA)  tktB → transketolase 2, thiamine triphosphate‑binding
RA 2,604,660 C→T 7.1% A199V (GCG→GTG)  hyfI → hydrogenase 4, Fe‑S subunit
RA 2,651,876 C→A 6.2% R140L (CGG→CTG)  hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,668,328 C→T 8.3% A265T (GCT→ACT)  yphC ← putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 2,674,853 T→A 9.5% R418W (AGG→TGG)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,694,758 C→A 8.2% G111W (GGG→TGG)  pdxJ ← pyridoxine 5'‑phosphate synthase
RA 2,706,831 G→T 6.8% G193C (GGC→TGC)  srmB → ATP‑dependent RNA helicase
RA 2,707,703 C→T 6.2% intergenic (+114/+95) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
RA 2,716,743 C→T 7.7% Q220* (CAG→TAG)  pssA → phosphatidylserine synthase (CDP‑diacylglycerol‑serine O‑phosphatidyltransferase)
RA 2,742,362 C→A 5.1% A77D (GCC→GAC)  yfjD → UPF0053 family inner membrane protein
RA 2,744,774 C→A 8.7% G195G (GGC→GGA nadK → NAD kinase
RA 2,755,636 G→T 7.3% H422N (CAT→AAT)  yfjK ← DEAD/H helicase‑like protein, CP4‑57 putative defective prophage
RA 2,761,401 A→G 5.7% Q111Q (CAA→CAG yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein
RA 2,762,683 C→G 10.7% T220T (ACC→ACG yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,762,686 T→C 9.5% Y221Y (TAT→TAC yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,762,692 G→T 12.1% R223R (CGG→CGT yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,762,698 A→G 7.3% Q225Q (CAA→CAG yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,762,701 A→G 7.4% E226E (GAA→GAG yfjQ → CP4‑57 prophage; uncharacterized protein
RA 2,769,263 C→G 5.1% intergenic (+88/‑15) ypjI → / → yfjX pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related/CP4‑57 prophage; putative antirestriction protein
RA 2,773,745 C→A 5.7% G781C (GGC→TGC)  ypjA ← adhesin‑like autotransporter
RA 2,811,690 G→T 5.8% intergenic (‑57/+142) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,821,058 G→T 9.9% G113V (GGA→GTA)  srlB → glucitol/sorbitol‑specific enzyme IIA component of PTS
RA 2,840,412 G→T 6.1% S120R (AGC→AGA hycD ← hydrogenase 3, membrane subunit
RA 2,881,535 C→A 6.5% G46V (GGT→GTT)  cysH ← phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
RA 2,890,377 C→A 5.4% A285S (GCC→TCC)  ygcS ← putative transporter
RA 2,926,845 T→A 9.8% K68M (AAG→ATG)  fucA ← L‑fuculose‑1‑phosphate aldolase
RA 2,929,140 T→G 8.3% A66A (GCT→GCG fucI → L‑fucose isomerase
RA 2,952,390 G→T 6.9% intergenic (‑147/+29) ptrA ← / ← recC protease III/exonuclease V (RecBCD complex), gamma chain
RA 2,955,579 C→A 7.3% W70L (TGG→TTG)  recC ← exonuclease V (RecBCD complex), gamma chain
RA 2,956,163 C→A 6.8% R118L (CGC→CTC)  ygdB ← DUF2509 family protein
RA 3,032,611 C→A 8.1% Q164H (CAG→CAT xerD ← site‑specific tyrosine recombinase
RA 3,033,025 C→A 5.3% L26F (TTG→TTT xerD ← site‑specific tyrosine recombinase
RA 3,044,764 C→A 6.2% G305C (GGC→TGC)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,050,481 G→T 10.2% intergenic (+134/+56) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
RA 3,058,267:1 +G 10.0% coding (107/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,270 T→A 10.0% L37Q (CTA→CAA) ‡ scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,271 A→G 9.5% L37L (CTA→CTG) ‡ scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,273 C→G 9.5% P38R (CCC→CGC)  scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:1 +A 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:2 +C 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:3 +G 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:4 +G 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:5 +C 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:6 +T 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:7 +G 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:8 +G 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:9 +A 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,058,277:10 +A 9.3% coding (117/1479 nt) scpC → propionyl‑CoA:succinate CoA transferase
RA 3,064,718 T→G 12.7% intergenic (‑115/+100) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,075,020 G→T 8.9% intergenic (‑26/‑252) tktA ← / → loiP transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
RA 3,092,667 C→A 8.0% L86L (CTG→CTT yggM ← DUF1202 family putative secreted protein
RA 3,109,788 G→T 12.0% P895Q (CCA→CAA)  yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,118,543 G→A 8.0% H92H (CAC→CAT glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
RA 3,167,253 C→A 5.1% L501L (CTG→CTT parE ← DNA topoisomerase IV, subunit B
RA 3,276,293 C→T 7.9% A320V (GCT→GTT)  agaS → tagatose‑6‑phosphate ketose/aldose isomerase
RA 3,280,250 G→T 12.9% E208* (GAA→TAA)  agaI → galactosamine‑6‑phosphate isomerase
RA 3,335,515 G→A 6.6% M297I (ATG→ATA kdsD → D‑arabinose 5‑phosphate isomerase
RA 3,343,116 C→A 7.3% R18L (CGG→CTG)  mtgA ← biosynthetic peptidoglycan transglycosylase
RA 3,366,851 G→T 5.4% R29S (CGC→AGC)  nanA ← N‑acetylneuraminate lyase
RA 3,416,890 T→G 8.6% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 10.6% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,443,328 C→A 9.4% A89S (GCA→TCA)  rplV ← 50S ribosomal subunit protein L22
RA 3,447,692 C→A 7.0% V356F (GTC→TTC)  gspA ← general secretory pathway component, cryptic
RA 3,452,360 G→T 12.9% L221F (TTG→TTT gspE → general secretory pathway component, cryptic
RA 3,453,002 G→T 7.2% V435V (GTG→GTT gspE → general secretory pathway component, cryptic
RA 3,472,095 C→A 8.4% Q19H (CAG→CAT yheV ← DUF2387 family putative metal‑binding protein
RA 3,514,916 G→T 11.4% T139K (ACG→AAG)  hofN ← DNA catabolic putative fimbrial assembly protein
RA 3,522,094 G→T 16.4% D23Y (GAC→TAC)  yrfG → GMP/IMP nucleotidase
RA 3,546,887 T→G 9.2% H148Q (CAT→CAG malT → mal regulon transcriptional activator
RA 3,563,413 C→T 9.5% D423N (GAT→AAT)  glgX ← glycogen debranching enzyme
RA 3,563,737 Δ1 bp 6.0% coding (943/1974 nt) glgX ← glycogen debranching enzyme
RA 3,563,738 Δ1 bp 6.1% coding (942/1974 nt) glgX ← glycogen debranching enzyme
RA 3,563,739 Δ1 bp 6.1% coding (941/1974 nt) glgX ← glycogen debranching enzyme
RA 3,616,061 T→C 5.2% Y1170Y (TAT→TAC rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,617,792 A→G 7.1% T19A (ACC→GCC)  yhhI → putative transposase
RA 3,621,656 G→A 8.2% R415W (CGG→TGG)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,623,359 C→A 5.3% E202* (GAA→TAA)  yhiI ← putative membrane fusion protein (MFP) of efflux pump
RA 3,630,062 C→A 6.5% G237* (GGA→TGA)  yhiN ← putative oxidoreductase
RA 3,635,229 G→T 5.0% L336L (CTG→CTT dtpB → dipeptide and tripeptide permease B
RA 3,637,377 G→T 13.7% P389Q (CCG→CAG)  prlC ← oligopeptidase A
RA 3,637,604 C→A 14.7% L313L (CTG→CTT prlC ← oligopeptidase A
RA 3,660,797 A→G 7.8% D48D (GAT→GAC gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,689,381 C→A 6.9% L55L (CTG→CTT yhjR ← DUF2629 family protein
RA 3,692,266 T→C 6.0% D231D (GAT→GAC bcsG → DUF3260 family inner membrane protein associated with cellulose production
RA 3,697,602 G→T 9.1% P174T (CCG→ACG)  dppC ← dipeptide/heme transporter
RA 3,716,679 G→T 6.2% T360K (ACG→AAG)  glyS ← glycine tRNA synthetase, beta subunit
RA 3,751,950 G→T 7.0% L424L (CTC→CTA selB ← selenocysteinyl‑tRNA‑specific translation factor
RA 3,766,302 C→T 10.5% P221L (CCA→CTA)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
RA 3,782,848 G→T 5.5% F198L (TTC→TTA waaH ← LPS(HepIII)‑glucuronic acid glycosyltransferase
RA 3,802,712 G→T 5.4% P271P (CCG→CCT waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
RA 3,836,309 C→A 7.1% G281C (GGC→TGC)  yicO ← putative adenine permease
RA 3,844,897 C→A 5.7% L416L (CTG→CTT ilvB ← acetolactate synthase 2 large subunit
RA 3,862,665 G→A 6.1% A2V (GCG→GTG)  yidR ← DUF3748 family protein
RA 3,869,765 C→T 6.2% S183N (AGT→AAT)  yidA ← sugar phosphate phosphatase; substrates include erythrose 4‑P and mannose 1‑P phosphatase
RA 3,904,197 G→A 7.0% A230V (GCG→GTG)  pstS ← periplasmic phosphate binding protein, high‑affinity
RA 3,935,371 G→T 9.7% noncoding (204/1542 nt) rrsC → 16S ribosomal RNA of rrnC operon
RA 3,937,442 G→T 5.7% noncoding (379/2904 nt) rrlC → 23S ribosomal RNA of rrnC operon
RA 3,977,282 C→A 8.2% P322Q (CCG→CAG)  aslB → putative AslA‑specific sulfatase‑maturating enzyme
RA 3,983,376 C→A 6.6% E251* (GAG→TAG)  hemC ← hydroxymethylbilane synthase
RA 4,005,046 G→T 6.1% W204L (TGG→TTG)  yigM → putative inner membrane EamA‑like transporter
RA 4,051,370 T→G 8.1% M9L (ATG→CTG)  glnA ← glutamine synthetase
RA 4,098,283 G→T 6.7% M268I (ATG→ATT pfkA → 6‑phosphofructokinase I
RA 4,098,583 G→A 13.6% intergenic (+141/‑179) pfkA → / → sbp 6‑phosphofructokinase I/sulfate transporter subunit
RA 4,098,599 Δ1 bp 10.9% intergenic (+157/‑163) pfkA → / → sbp 6‑phosphofructokinase I/sulfate transporter subunit
RA 4,099,484 C→A 21.6% T241T (ACC→ACA sbp → sulfate transporter subunit
RA 4,120,021 A→G 7.9% S87G (AGC→GGC)  metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,120,084 C→A 6.3% R108S (CGC→AGC)  metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,135,984 C→T 6.5% L688L (CTG→TTG)  pflD → putative glycine radical domain‑containing pyruvate formate‑lyase
RA 4,164,754 G→T 6.6% S67R (AGC→AGA coaA ← pantothenate kinase
RA 4,165,449 C→T 25.7% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 25.6% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,166,006 C→T 6.2% R45R (CGC→CGT tufB → translation elongation factor EF‑Tu 2
RA 4,176,460 A→G 8.6% K395E (AAA→GAA)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 61.7% R1075C (CGT→TGT) ‡ rpoC → RNA polymerase, beta prime subunit
RA 4,178,501 G→T 30.9% R1075L (CGT→CTT) ‡ rpoC → RNA polymerase, beta prime subunit
RA 4,188,787 G→T 6.4% D24Y (GAT→TAT)  nfi → endonuclease V; deoxyinosine 3' endonuclease
RA 4,208,252 G→T 5.1% G406C (GGT→TGT)  aceA → isocitrate lyase
RA 4,271,272 G→T 8.0% E455D (GAG→GAT yjcE → putative cation/proton antiporter
RA 4,283,506 A→G 6.9% T12T (ACA→ACG nrfG → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG
RA 4,287,172 G→T 8.4% P671Q (CCG→CAG)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit
RA 4,296,881 C→A 11.1% G246C (GGT→TGT)  alsK ← D‑allose kinase
RA 4,306,837 G→T 14.7% P67T (CCG→ACG)  phnM ← ribophosphonate triphosphate hydrolase
RA 4,313,212 G→T 6.4% L320M (CTG→ATG)  phnD ← phosphonate ABC transporter periplasmic binding protein
RA 4,340,756 C→T 7.8% G242S (GGC→AGC)  dcuS ← sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
RA 4,369,894 G→T 6.3% S147S (TCC→TCA frdB ← fumarate reductase (anaerobic), Fe‑S subunit
RA 4,376,949 G→T 9.0% R747S (CGT→AGT)  mscM ← mechanosensitive channel protein, miniconductance
RA 4,381,059 C→A 8.9% G90C (GGC→TGC)  rsgA ← ribosome small subunit‑dependent GTPase A
RA 4,384,645 G→T 7.5% G255W (GGG→TGG)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
RA 4,398,586 G→A 5.1% V706M (GTG→ATG)  rnr → exoribonuclease R, RNase R
RA 4,407,614 G→A 5.8% S219S (AGC→AGT ulaR ← transcriptional repressor for the L‑ascorbate utilization (ula) divergent operon
RA 4,407,629 C→A 5.9% A214A (GCG→GCT ulaR ← transcriptional repressor for the L‑ascorbate utilization (ula) divergent operon
RA 4,417,101 C→A 12.2% D47Y (GAT→TAT)  yjfZ ← uncharacterized protein
RA 4,425,105 T→C 5.7% K426K (AAA→AAG cpdB ← 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase
RA 4,467,913 A→C 8.1% A72A (GCT→GCG argI ← ornithine carbamoyltransferase 1
RA 4,467,916 A→G 8.8% G71G (GGT→GGC argI ← ornithine carbamoyltransferase 1
RA 4,467,926 T→A 10.2% Y68F (TAT→TTT)  argI ← ornithine carbamoyltransferase 1
RA 4,492,706 G→T 6.5% A181A (GCC→GCA insG ← IS4 transposase
RA 4,508,885 T→A 5.4% pseudogene (348/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,520,242 G→T 5.4% Q37K (CAA→AAA)  sgcB ← putative enzyme IIB component of PTS
RA 4,522,243 G→T 7.4% intergenic (‑116/+11) yjhY ← / ← yjhP pseudogene, zinc finger protein family/putative methyltransferase
RA 4,575,139 C→A 9.7% pseudogene (1494/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,575,665 A→G 7.1% pseudogene (968/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R
RA 4,604,709 G→T 11.9% Q218K (CAG→AAG)  yjjW ← putative pyruvate formate lyase activating enzyme
RA 4,623,132 G→A 8.2% T144I (ACC→ATC)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 578863 579101 239 10 [8] [7] 9 tfaX/appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
* * ÷ CP009273 1524289 1524468 180 9 [8] [8] 10 ydcD uncharacterized protein
* * ÷ CP009273 3448766 3448865 100 9 [8] [4] 18 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ CP009273 3792502 3792785 284 12 [8] [3] 9 [waaU]–[waaZ] [waaU],[waaZ]
* * ÷ CP009273 3797648 3797871 224 9 [4] [8] 10 waaS lipopolysaccharide core biosynthesis protein
* * ÷ CP009273 4249415 4249935 521 9 [7] [7] 10 zur zur

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =40 (0.790)27 (0.530) 16/250 NT 40.3% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =31 (0.610)14 (0.320) 10/218 NT 33.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 29 (0.660)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326135 (0.690)27 (0.610) 12/218 NT 47.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 29 (0.660)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 2008478 =59 (1.170)4 (0.080) 3/246 NT 5.6% coding (118/996 nt) fliG flagellar motor switching and energizing component
?CP009273 = 2008634 76 (1.530)coding (274/996 nt) fliG flagellar motor switching and energizing component
* ? CP009273 = 2283592NA (NA)47 (0.940) 26/246 NT 90.4% noncoding (1/1195 nt) IS5 repeat region
?CP009273 2399636 = 5 (0.100)coding (485/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
* ? CP009273 = 23996395 (0.100)51 (1.010) 27/250 NT 91.1% coding (482/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
?CP009273 = 3124699 NA (NA)intergenic (+146/+1) insH1/yghQ IS5 transposase and trans‑activator/putative inner membrane polysaccharide flippase
* ? CP009273 3809368 =72 (1.420)7 (0.140) 3/248 NT 8.5% pseudogene (542/669 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
?CP009273 = 3809638 80 (1.590)pseudogene (272/669 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH