Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,910 A→G 31.3% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9100AG31.3% 147.8 / 107.0 128intergenic (‑1/‑121)USA300HOU_0653/USA300HOU_0654ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (43/45);  new base G (16/24);  total (59/69)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

Only 100 of 131 total aligned reads displayed.

TTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAG  >  CP000730/707773‑708049
                                                                                                                                         |                                                                                                                                           
ttGATAAAGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTACTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGt                                                                                                                                          <  1:989159/141‑1 (MQ=255)
  gATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAATTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGttt                                                                                                                                        <  1:797823/141‑1 (MQ=255)
   aTAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATGATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTTCATGCTTATTACCTAAAAATAGATTTAAATCTTTTGTTTCTAACATACGTTta                                                                                                                                       >  2:655757/1‑141 (MQ=255)
    tAAGGGAAGATTTACCAGCACCATTCGGGCCCATGCTACCAATGATGTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTtat                                                                                                                                      <  1:220607/141‑1 (MQ=255)
    tAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTAACTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTtat                                                                                                                                      <  2:491516/141‑1 (MQ=255)
     aaGGGAAGATGTACCAGCACCATTCGGGCCCATGATACCAATTTTTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTtata                                                                                                                                     <  2:56386/141‑1 (MQ=255)
        ggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTCATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcct                                                                                                                                  <  1:1091156/141‑1 (MQ=255)
            gATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTa                                                                                                                              >  2:833123/1‑141 (MQ=255)
            gATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTa                                                                                                                              <  1:734836/141‑1 (MQ=255)
             ttttACCAGCACCACTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTaa                                                                                                                             <  2:1120219/140‑1 (MQ=255)
             aTTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCGTTTGTTTCTAACAAGCGGTTGTACCTCCTaa                                                                                                                             >  1:82166/1‑141 (MQ=255)
             aTTTACCAGAACCATTCGTGCCCATGATACCAATTATTTCGCCGCGTAATGGTATCTATAAAGAAATGTTTTTAAGAACATAAAAAAAACAAAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTaa                                                                                                                             <  2:458525/141‑1 (MQ=255)
              tttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTTTACCTCCTAAt                                                                                                                            >  1:906069/1‑141 (MQ=255)
              tttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAAt                                                                                                                            <  2:1136571/141‑1 (MQ=255)
              tttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAAt                                                                                                                            <  2:468440/141‑1 (MQ=255)
               ttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAAtt                                                                                                                           >  1:1158851/1‑141 (MQ=255)
                tACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTCTAACTCCTAATTa                                                                                                                          >  2:269054/1‑141 (MQ=255)
                  ccAGGACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaaa                                                                                                                        <  2:298884/141‑1 (MQ=255)
                     gCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGt                                                                                                                     <  1:340557/141‑1 (MQ=255)
                      cACCATTCGGGCCCATGATCCACTTTCTTGCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGtt                                                                                                                    >  1:880658/1‑141 (MQ=255)
                       aCCATTCGGGCCGATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACTTAAAAACAGATTTAAATCTTTTGTTTCTAACCTACGTTTATACCTCCTAATTAAAAGttt                                                                                                                   >  1:617646/1‑141 (MQ=255)
                        ccATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTa                                                                                                                  >  2:508559/1‑141 (MQ=255)
                            tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTAGCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCt                                                                                                              >  1:51151/1‑141 (MQ=255)
                              gggCCCATGATACCGATTAGTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGGACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATGCGTTTATACGTCCTAATTAACAGTTTAGGCTaa                                                                                                            >  2:668057/1‑141 (MQ=255)
                               ggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAc                                                                                                           >  1:1119331/1‑141 (MQ=255)
                               ggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAc                                                                                                           >  1:1119172/1‑141 (MQ=255)
                                gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                          <  1:988515/141‑1 (MQ=255)
                                gCCCAGGATGCCGAGTAGTGCGGCGCGTACTGGTATCGATAAGGAAAGGGTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                          <  2:719381/141‑1 (MQ=255)
                                  caaTGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCta                                                                                                        <  2:958328/139‑1 (MQ=255)
                                   cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                       <  2:1158851/141‑1 (MQ=255)
                                       aTACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaattaa                                                                                                   >  1:1148843/1‑141 (MQ=255)
                                          ccAATTATTTCGCCGGGGTCTGGTATCTATAATGAAATTTTTTTAAGGACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCACCTAACTAAAAGTTTAGGCTAACCTAATTAATTg                                                                                                <  1:284125/141‑1 (MQ=255)
                                           cAATTATTCCGCCGCGTACTGGTAGCGATAAGGAAATGTTTTTTCGTTCACGCGTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                                                               <  2:67897/141‑1 (MQ=255)
                                             attattTCTGCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtat                                                                                             <  2:466988/141‑1 (MQ=255)
                                              ttattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                                                            <  2:762722/141‑1 (MQ=255)
                                               tattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGGTAACCTAATTAATTGTataa                                                                                           <  1:728584/141‑1 (MQ=255)
                                                  ttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTAtaataa                                                                                        >  2:1173504/1‑141 (MQ=255)
                                                  ttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTAtaataa                                                                                        >  2:1173635/1‑141 (MQ=255)
                                                  ttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATAAGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTAtaataa                                                                                        >  2:556635/1‑141 (MQ=255)
                                                     gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                                                     <  2:446762/141‑1 (MQ=255)
                                                      ccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTg                                                                                    >  1:419250/1‑141 (MQ=255)
                                                         cgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                                                                 <  1:995208/141‑1 (MQ=255)
                                                           tACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAat                                                                               <  2:419555/141‑1 (MQ=255)
                                                            aCTGGTTCCGATAAGTTAATTTTTTTAAGTACATGCTTATTACATTAAATCAGATTAAATTCTTTTGTTTCTAACATACGTTTATACCTCCAAATCAAAGATTTAGACTAACCTAATTAATTGTATCATAAAGTGAGtata                                                                              <  2:370751/141‑1 (MQ=255)
                                                             cTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAatat                                                                             <  1:949030/141‑1 (MQ=255)
                                                               ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                                                           >  1:451973/1‑141 (MQ=255)
                                                               ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                                                           <  2:960743/141‑1 (MQ=255)
                                                               ggTATCCATAAGGAAATGTTTTTAAGTACATGAAAAAAAAAAAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                                                           <  2:960987/141‑1 (MQ=255)
                                                                 tATCGATAAGGAAATGTTTTTAAGTACATGCTTATTCCCTAAAAACAGATTTAAGTCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGTTAACCTAATTAATTGTATGATCAACTGAGAATATTTAt                                                                         <  1:668057/141‑1 (MQ=255)
                                                                  aTCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATc                                                                        <  2:1119172/141‑1 (MQ=255)
                                                                        aaGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCa                                                                  <  2:291379/141‑1 (MQ=255)
                                                                          ggAAATGTTTTTAAGTACATGCTTATTACCTAAATACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTAATAATTAAAAGTTTATGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTTAAg                                                                <  2:877/141‑1 (MQ=255)
                                                                          ggAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                                                                <  1:35093/141‑1 (MQ=255)
                                                                           gAAATGTTCTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTAAAGt                                                               >  2:889206/1‑141 (MQ=255)
                                                                            aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                              <  2:880658/141‑1 (MQ=255)
                                                                             aaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATTATGTCAAGTaa                                                             >  1:960154/1‑141 (MQ=255)
                                                                              aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATACACGGAGAATATTTATCATTTCAAGTaaa                                                            >  1:211348/1‑141 (MQ=255)
                                                                              aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                            >  2:1076169/1‑141 (MQ=255)
                                                                              aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                            <  2:1022251/141‑1 (MQ=255)
                                                                                   tttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTCAGGATAACCTAATTAATTGTATACTAAACTGAGTATATGTATCAAGACAAGTAAAttatt                                                       >  2:1086535/1‑138 (MQ=255)
                                                                                   tttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTGATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACGGGGAATATTTATCATTTCAAGTAATTTTGt                                                       >  2:719160/1‑141 (MQ=255)
                                                                                   tttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                                                       >  2:760396/1‑141 (MQ=255)
                                                                                   tttAAGTACAGGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTGGt                                                       >  2:734325/1‑141 (MQ=255)
                                                                                    ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                                      <  1:703445/141‑1 (MQ=255)
                                                                                      aaGTACCTTCTTTGTACCTAAAAACAGATTTAAATCTTTTTTTTCTAACATACGTTTATACCTCCTAATTAAAAGGTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGat                                                    <  2:703821/141‑1 (MQ=255)
                                                                                      aaGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGat                                                    <  2:222872/141‑1 (MQ=255)
                                                                                          aCATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAGTTCGTGatataa                                                >  1:910088/1‑141 (MQ=255)
                                                                                                 tattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAc                                         >  2:288890/1‑141 (MQ=255)
                                                                                                   ttaCCTAAAAACGGAGTTACATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTTTAATAAACTGAAAATATTTATCATGTCAAGTAAATACGATAATTAATGTAGACaa                                       >  2:437116/1‑141 (MQ=255)
                                                                                                   ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa                                       <  1:1076169/141‑1 (MQ=255)
                                                                                                      ccTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgt                                    >  2:762528/1‑141 (MQ=255)
                                                                                                       cTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAATCAATTCTTGATGTAATATAGACAattta                                   >  1:268958/1‑141 (MQ=255)
                                                                                                       cTAAAAACAGATTTAAATCTTTTGCAATGAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgta                                   >  1:285443/1‑141 (MQ=255)
                                                                                                          aaaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCATAATTAGAAGTTTAGGCTAATCTAGTTAATTTTATAATAAACTCAGTCTATTTATCATGTCGAGTAAATTGGTGTTATTATAAAgggatggtgtgt                                >  2:3856/1‑130 (MQ=255)
                                                                                                          aaaaCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatgt                                >  1:892869/1‑141 (MQ=255)
                                                                                                           aaaCAGATTTAAATCTTTTGTTGCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAAAATGTAtgtg                               <  2:206916/141‑1 (MQ=255)
                                                                                                            aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTGTAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGa                              >  2:940350/1‑141 (MQ=255)
                                                                                                            aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGa                              >  2:977372/1‑141 (MQ=255)
                                                                                                            aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGa                              >  1:107378/1‑141 (MQ=255)
                                                                                                            aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAAGTATgaga                              >  1:193307/1‑141 (MQ=255)
                                                                                                            aaCAGATTTAAATCTTTTGTTTCTAACATACGTTCATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTAGTGATATAATATAGACAAAGGAtgtgg                              >  1:249966/1‑140 (MQ=255)
                                                                                                              cAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                            <  1:918845/141‑1 (MQ=255)
                                                                                                                gATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgggt                          >  1:367869/1‑139 (MQ=255)
                                                                                                                gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgggg                          >  2:827098/1‑139 (MQ=255)
                                                                                                                   ttAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAGTAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGaaa                       <  2:1199864/141‑1 (MQ=255)
                                                                                                                     aaaTCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGt                     >  2:584138/1‑141 (MQ=255)
                                                                                                                      aaTCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTa                    >  2:17862/1‑141 (MQ=255)
                                                                                                                       aTCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTAAAGTAAATTCGTGATATAATATAGACAATGTATGAGGGGGGAAAGTAt                   >  1:611675/1‑141 (MQ=255)
                                                                                                                        tCTTTTGGTTCTAACCAGCTTTTATAACTCCTAACTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATg                  <  1:960990/141‑1 (MQ=255)
                                                                                                                           tttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTGATTGTGTAATAAACTGAGAATATTTATCATGTGAAGTAAATTGGTGATATAATATAGACAATGTATGGGAGGTGAAAGTGTGTTa               >  1:22822/1‑141 (MQ=255)
                                                                                                                              ttttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCATGATATAATATAGACAATGTATGTCAGGTGAAAGTATGTTAACt            <  1:655757/140‑1 (MQ=255)
                                                                                                                              gTTTCTAACATACGTTTATACCTCCTAATTAAGAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACt            >  1:857421/1‑141 (MQ=255)
                                                                                                                              gTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGTGGTGAAAGTATGTTAACt            >  2:921146/1‑141 (MQ=255)
                                                                                                                              gTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACt            >  1:1031223/1‑141 (MQ=255)
                                                                                                                                ttCTAGCATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga          <  2:501978/141‑1 (MQ=255)
                                                                                                                                ttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga          <  2:501971/141‑1 (MQ=255)
                                                                                                                                tgcTAGCATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga          <  1:1166910/139‑1 (MQ=255)
                                                                                                                                    aaCATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaagaa      <  1:833123/141‑1 (MQ=255)
                                                                                                                                        taCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGCAGaaaacg  >  2:351353/2‑139 (MQ=255)
                                                                                                                                        taCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAag  >  2:824765/2‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAAAAG  >  CP000730/707773‑708049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: