Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,6920CA41.8% 84.3 / 27.6 55intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (2/30);  new base A (23/0);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.61e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

AAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903550‑2903841
                                                                                                                                              |                                                                                                                                                         
aaGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCAtt                                                                                                                                                   <  1:355315/151‑1 (MQ=255)
 aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTACAACCACTCATTa                                                                                                                                                  <  1:218335/151‑1 (MQ=255)
          aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaa                                                                                                                                        <  1:28700/150‑1 (MQ=255)
             tACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACACTCCTTATCCTCaaaa                                                                                                                                       >  1:502560/1‑148 (MQ=255)
                 cTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCATTATACTCCAaaaaaaaa                                                                                                                                 >  2:107049/1‑148 (MQ=255)
                     aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAAc                                                                                                                           <  1:378833/151‑1 (MQ=255)
                         cTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTTAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACTCATTAGCCTCCAaaaaaaaaaaaa                                                                                                                            >  2:257595/1‑146 (MQ=255)
                                       aCAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTa                                                                                                         <  1:313251/151‑1 (MQ=255)
                                         aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTTAAAAACACATTATACTCAAAACAAAAAAAAAATTCCAGATTTATTAttt                                                                                                          >  1:47317/1‑148 (MQ=255)
                                            aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCa                                                                                                     <  2:859027/150‑1 (MQ=255)
                                            aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCa                                                                                                     <  2:134133/150‑1 (MQ=255)
                                               aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTAACCTCAAAAAAAAAAAAAAATTTCAGATTTTTTACTTAAACAATg                                                                                                  >  2:152286/1‑150 (MQ=255)
                                                aTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGaa                                                                                                <  1:474039/151‑1 (MQ=255)
                                                  cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATa                                                                                              <  2:356949/151‑1 (MQ=255)
                                                       tttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAAATCATTTACCCCACAAAAAAAAAAAAAATCCAGATTTATTATTTAACACATGAATAaaaa                                                                                          >  1:720743/1‑150 (MQ=255)
                                                            cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGTTTGTGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAATCTTa                                                                                    <  1:868208/150‑1 (MQ=255)
                                                            cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTa                                                                                    <  2:412437/150‑1 (MQ=255)
                                                              aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaaa                                                                                 <  1:338402/151‑1 (MQ=255)
                                                                  tCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACTCATTATCCTCCAAAAAAAAACAAAATTCCAGATTTTTTTCTTAAACAAAGAATAAAAAACTTaaaaaa                                                                               >  2:516768/1‑149 (MQ=255)
                                                                   cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACACCCCTTAACCTCCAAAAAAAAAAAAAATTCCAGATTTTTTTCTTTAACAAATAATAACAAACTTaaaaaaaa                                                                             >  2:601152/1‑148 (MQ=255)
                                                                     cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAAAACTCATTTACCTCCAAAAAAAAAAAAAAATTCCAATTTTTTTATTTACCAAAAAAAAAAAAACATaaaaaaa                                                                              >  2:321265/1‑146 (MQ=255)
                                                                       tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTATAAAAACAATCCCTTCTCTCAAAAAAAAAAAAAAACTTCCGATTTTTTTCTTTACAAAAAAAAAAAAAACATTaaaaaa                                                                               >  2:323973/1‑145 (MQ=255)
                                                                       tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACt                                                                         <  1:453594/150‑1 (MQ=255)
                                                                           gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACAATCATCTTCCTCAAAAAAAAAAAAAAAATTCCGAATTTTTTATTTACCAAAAAAAAAAAAGATTaaaaaaaaaaatttttt                                                                    >  1:1184/1‑140 (MQ=255)
                                                                           gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAt                                                                    <  2:835449/151‑1 (MQ=255)
                                                                              tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTTAAAACACACCTTATCCTCAAAAAAAAAAAAAAAATTCAGAATTTTTTTTTTAACAAAGAAAAAAAAAACTAAAAAAAATAATGTTAtttt                                                                 >  2:421879/1‑149 (MQ=255)
                                                                                    ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACTCCTTATACTCAAAAAAAAAAAAAAATTCCAAATTTATTACTTAAAAAAAAAATAAAAAACATAAAAAAAATAATTTTTTTTTaaa                                                              >  1:395301/1‑148 (MQ=255)
                                                                                      atatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAACTCATTATCCTCAAAAAAAAGACAAAATTCCAGATTTTTTTCTTAAACAAAGAATAAAAAAATTAAAAAAAAAAATTTTATTTTTaaaaaa                                                          >  1:402495/1‑150 (MQ=255)
                                                                                       tatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACACCTTAAGCTCAAAAAAAAAACAAAACTTCCGATTTTTTTTTTAAACAAAGAAAAAAAAAACTAAAAAAAATAATTTTTTTTTaaaaaaaa                                                         >  2:123775/1‑150 (MQ=255)
                                                                                        atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCATTATCCCCCAAAAAAAAAAAAAAATCCAGATTTATTTTTTTAACAAAAAAAAAAAAAATTTAAAAAAAAAATGTTTTTTTAaaaaaaaa                                                        >  2:410083/1‑150 (MQ=255)
                                                                                        atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaa                                                        <  2:695688/150‑1 (MQ=255)
                                                                                        atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaa                                                        <  2:446365/150‑1 (MQ=255)
                                                                                           tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACACTCATTAACCTCAAAAAAAAAAAAAAAATCCAGATTTATTTATTTAACAAAAAAAAAAAAACTTAAAAAAAATAATTTTATTTTTAaaaaaaaaa                                                      >  2:262241/1‑148 (MQ=255)
                                                                                            ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACAACCATTAGCCTCAAAAAAAAAAAAAACTTCCAGATTTTTTTCTTTAACAAAAAAAAAAAAACATAAAAAAAATAATGTTATTTTaaaaaaaaaaa                                                      >  2:558592/1‑147 (MQ=255)
                                                                                            ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAAt                                                      <  2:811990/148‑1 (MQ=255)
                                                                                             tATTCATAAGAACTATTCATCAACAGAGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTTCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATCTACTGTTATTCTAAAGaaaaaa                                                       <  1:86825/146‑1 (MQ=255)
                                                                                              aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTACCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAAc                                                  <  1:688330/150‑1 (MQ=255)
                                                                                               ttCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACTCATTAACCTCCAAAAAAAAACAAAACTTCAGATTTTTTAATTAAACAAAGAAAAAAAAACTTAAAAAAAATAATTTTATTTTAAAAAAAAAAATaa                                                   >  2:252352/1‑148 (MQ=255)
                                                                                                tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAg                                                <  1:407665/150‑1 (MQ=255)
                                                                                                  aTAAGAACTATTCATCAACAGCGTATAGCGGCGGTGATATAAAAAAACTCTTTAATCTCTAAATAAAAAAAAAGATTAAGAATTATTATTTTATCAATTAAAAAAAAAACTACAAAAAAAAAAGTTTTTTTaaaaaaaaaaattactgg                                               >  1:504833/1‑141 (MQ=255)
                                                                                                    aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt                                           <  1:279184/151‑1 (MQ=255)
                                                                                                       aaCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAt                                        <  1:478253/151‑1 (MQ=255)
                                                                                                        aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt                                       <  1:733641/151‑1 (MQ=255)
                                                                                                          tATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTTACCTCAAAAAAAAAAAAAAATTTCCAATTTATTTCTTAAACAAAAAAAAAAAAACTTAAAAAAAATAATTTTTTTTTAAAAAAAAAAATAAAAGGCAATAAtt                                       >  2:765087/1‑149 (MQ=255)
                                                                                                           aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                      <  2:432295/149‑1 (MQ=255)
                                                                                                           aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                      <  2:68254/149‑1 (MQ=255)
                                                                                                           aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAACATACTGTTAATCTAAAGAAAAAATTAACAGGCATTAATTCa                                     <  1:820899/150‑1 (MQ=255)
                                                                                                            ttCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCaa                                    <  2:502689/150‑1 (MQ=255)
                                                                                                             tcatcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat                                   <  2:747927/150‑1 (MQ=255)
                                                                                                               atcaACAGCGTATAGAGGCGGTTTTGTAAAAACACTAATTATCCTCCAAAAAAAAACAAAAATCCATAATTTTTATTTATACAAAAAAAAAAAAAAATAAAAAAAATAATTTTTTTTTTAAAAAAAAAATAAAAAAAATTAATTTaaaa                                  >  1:827419/1‑147 (MQ=255)
                                                                                                                     aGCGTATAGAGGCGGTTATGTAAAAACACCCATTAGACTCAAAAAAAAAAAAAACTTCCAGATTTTTTACTTTAACAAAGAAAAAAAAACTTAAAAAAAATACTTTTATTTTTAAGAAAAAAATAAAAAGCATTAATTTAATATTtaaaa                           >  2:302302/1‑150 (MQ=255)
                                                                                                                       cGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaaa                         <  1:513659/150‑1 (MQ=255)
                                                                                                                             gagGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                     >  1:361590/1‑148 (MQ=255)
                                                                                                                                            aaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc   <  2:718949/151‑1 (MQ=255)
                                                                                                                                            aaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc   >  1:83705/1‑151 (MQ=255)
                                                                                                                                              ccACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg  >  2:485679/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                         
AAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903550‑2903841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: