Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,133,302 | 0 | T | G | 41.1% | 98.1 / 13.9 | 56 | H219P (CAC→CCC) | yghS | putative ATP‑binding protein |
Reads supporting (aligned to +/- strand): ref base T (3/30); new base G (23/0); total (26/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.21e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAAT > NC_000913/3133152‑3133445 | gAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgt < 1:287205/151‑1 (MQ=255) tGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCa < 2:851181/150‑1 (MQ=255) gggCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCcccccac > 2:383486/1‑148 (MQ=255) gCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCTGCGGGGTGTCGCCCATCCa < 1:491553/151‑1 (MQ=255) cgcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCCTCCCG‑ttt > 1:57866/1‑147 (MQ=255) gcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAgg < 2:69474/151‑1 (MQ=255) ggTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCG‑TTccaaggt > 1:134021/1‑145 (MQ=255) gTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCC‑ATC‑TAGGATCtt > 1:262013/1‑150 (MQ=255) tACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCTCCCCCAG‑TTccaagagtctt > 1:504588/1‑140 (MQ=255) tACTATTAACAGGTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAG‑CTC‑AAGGATCttt > 1:253501/1‑150 (MQ=255) aTTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTgcg < 1:575372/149‑1 (MQ=255) tAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCa < 1:482796/150‑1 (MQ=255) aCAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTg < 2:839910/151‑1 (MQ=255) cAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGa < 1:597022/151‑1 (MQ=255) agtttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCATCCCG‑TTC‑CAGGGTCTTTTCGCCAtt > 2:821658/1‑148 (MQ=255) tgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCG‑TTC‑AAGGATCTTTTCGCCCTTTAATGt > 2:339625/1‑150 (MQ=255) agtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAG‑TTC‑AAGGATCTTTTCGCCATTTAATGTc > 2:633030/1‑149 (MQ=255) tttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAG‑TTC‑AAGGGTCTTTTCTCCCTTTTATGTCAGtt > 1:792388/1‑151 (MQ=255) tgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGtt < 1:644558/149‑1 (MQ=255) tgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCCACCCG‑ATC‑AAGGATCTTTTTGCCATTGGATGTCAggt > 1:848464/1‑147 (MQ=255) gTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTgc < 2:720309/150‑1 (MQ=255) gCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGt < 1:446426/150‑1 (MQ=255) cATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTaa < 2:107303/151‑1 (MQ=255) ccATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACg < 2:702591/150‑1 (MQ=255) gTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAttt < 2:372201/151‑1 (MQ=255) gTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGATTC‑AGGGAT‑TTTGGTCCATTTAATGGCGGGTGCGGCGGTACGGCGGttt > 1:853750/1‑151 (MQ=255) aaaTCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGAttttt < 1:232820/150‑1 (MQ=255) atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTcc < 1:181283/151‑1 (MQ=255) atcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGt < 2:176554/151‑1 (MQ=255) ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCCCCCCTCCAA‑CTCAAAGAATCTTTG‑GCCATTTTATGTCCGCTGCGGCGCTAAGGCGGTTTTTTTCTGtt > 2:368184/1‑149 (MQ=255) tATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAG‑TTT‑AAGGATCTTTTCGCCATTGGATGGTAGTTGCGGGGTAACGGCGGTTTTTTCCTGttaag > 2:764134/1‑145 (MQ=255) cATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCa < 1:311929/151‑1 (MQ=255) tGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCG‑TTC‑AAGGAACTTTGCGCCCATGGATGTTCGTTGTGGCGGAACGGCGGTTTTTTTCTTTCACgccgcccggt > 1:38563/1‑145 (MQ=255) tGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCG‑TTC‑AAGGATCTTTTCGCCATTGAATGTCAGTTGCGGCGGAACGGCGGTTTTTTTCTGTAAAGCTGCCCGtt > 2:22684/1‑149 (MQ=255) ggTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtggt < 1:486816/151‑1 (MQ=255) ggTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtggt < 1:131832/151‑1 (MQ=255) aTCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCCTCCAG‑ATC‑AAGGATCTTTGCGCCATTTAATGGCAGTTGCGGGGTAACGGCGGGTTTTTTCTGTAACGCCGCCAGTtgggggg > 1:732914/1‑149 (MQ=255) tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAG‑GTC‑AAGGATCTTTGCGCCATTTAATGTCAGTTGCGGCGGAACGGCGGTTTTTTTCTGTAACGCTGCCCGGtggtgg > 1:118458/1‑148 (MQ=255) aGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAgg < 2:92838/151‑1 (MQ=255) gAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGtt < 1:600299/151‑1 (MQ=255) aGCGCGGCGTGAATTGCGCGTATTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTAc < 1:1568/151‑1 (MQ=255) gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTAcg < 1:837942/151‑1 (MQ=255) cgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAG‑TTT‑AAGGATATTTGCGCCATTTAATGTCAGTTGCGGGGTAACGGCGATTTTTTCCTTTAAAGCTGCCCGTTGGGGGGCCGGGTTTCgcgc > 2:674687/1‑148 (MQ=255) gTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCCTCCCG‑TTC‑AAAGGAATTTTCGCCACTGAATGTCAGATGAGGGGTAACCGCGGTTTTTTCCTGTAAAGACGCCCGCTGGTGGGCAGGGTTGCGCGCaa > 1:443455/1‑149 (MQ=255) aTTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCgg < 2:792599/151‑1 (MQ=255) gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGttt < 2:75039/151‑1 (MQ=255) gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGtt < 1:339526/150‑1 (MQ=255) gcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCATCCAG‑GTC‑AAGGAGATTTGCGCCATTGTATGTGAGTTGCGGCGCAACGGCGATTTTTTTCTGTAAAGCTGCCCGGTGGGGGGGAGGGTTACGCGCAAAAGCGGTTTGt > 1:739504/1‑151 (MQ=255) cgTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGtt < 1:436731/151‑1 (MQ=255) aGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtca < 2:695614/150‑1 (MQ=255) tGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAA‑TTC‑AAAGAACTTTGCGCCATTTAATGTCAGTTGGCGCGTAAAGGCGAATTTTTGCTGTAAAGCTGCCAGTTGGGGGGTAGGTTTACGCGCCAAAGCGGCTTGTtca > 2:501046/1‑148 (MQ=255) aCGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAG‑TTT‑AAGGATCTTTTCGCCATTGAATGTCAGTTGCGGGGTAAAGGCGGTTTTTTTCTGTAACGCTGCCAGGTGGGGGGCAGGGTTAAGCGCAAACGCGGGTTGTtcatca > 2:328012/1‑149 (MQ=255) cGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATg < 1:48369/151‑1 (MQ=255) cGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAG‑TTC‑AAGGATCTTTTCGCCATTGAATGTCAGTTGCGGCGGAACGGCGATTTTTTTCTGTAACGCTGCCAGTTGGGGGGCAGGGTTACGCGCAAAAGCGGTTTGTTCATCaa > 2:615250/1‑149 (MQ=255) ttGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAG‑TTC‑AAGGATCTTTTCGCCCTTGAATGTCAGTTGCGGGGTAACGGCGGTTTTTTTCTGTAACGCTGCCAGTTGGTGGGCCGGTTTACGCGCAAAAGCGGGTTGTTTATCAATGcc > 2:306173/1‑150 (MQ=255) tGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCa < 1:392782/150‑1 (MQ=255) ggcggGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCCAGACGAATCAACAAt > 1:632877/1‑151 (MQ=255) | GAATGTAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAG‑TTC‑AAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAAT > NC_000913/3133152‑3133445 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |