| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,825,056 | 0 | G | A | 44.0% | 63.5 / 24.3 | 50 | intergenic (‑119/+84) | ves/spy | cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced |
| Reads supporting (aligned to +/- strand): ref base G (25/3); new base A (0/22); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.18e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA > NC_000913/1824909‑1825202 | gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTgagaagagaa > 2:387855/1‑150 (MQ=255)gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTgagaagagaa > 1:566540/1‑150 (MQ=255) tttACGCATATCAAAGTATTCCATACTCCCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGCGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGagaaaa > 1:407714/1‑147 (MQ=255) gCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAAccgc > 1:190961/1‑151 (MQ=255) cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAAccgcc < 1:494/151‑1 (MQ=255) cATATCAAAGTATTCCATACTCACTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAAccgc > 2:32576/1‑150 (MQ=255) tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAAccgccg > 1:703164/1‑150 (MQ=255) tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTCACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATc > 1:664850/1‑151 (MQ=255) gTATTCCATACTCGCTCCCGGCCCTTTTTTTTCATTGTTATCGTTCAGGTACCCGTGGATGTTTATGGGCAACTCGGGTGATTTACTTTAGTGGTGATCAGGTTTTTTTTACAT‑CTGTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGt < 2:318020/150‑1 (MQ=255) tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc > 2:126216/1‑150 (MQ=255) cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc > 2:128301/1‑151 (MQ=255) cGCTCCGGGCCCGTTTTTTTATTTGTTTTCGTTCAGGAACCGGTGGTTGTTTATCGACAACTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAC‑CTGTTTAAGAAGGAAAAAGAAAACCGCCGATCCTGTCCACCGCATTa < 1:148930/149‑1 (MQ=255) cccGCCCTGTTTTTTTCTTTGTTATCGTTAGGGTACCCGTGGATGTTTATCGCCACCTGGGTTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAC‑CTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCa < 2:588954/150‑1 (MQ=255) cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg > 1:730407/1‑151 (MQ=255) ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg > 1:424734/1‑150 (MQ=255) ttCTTTTTTATCGTTCAGGTAACCGGGGATGGTTTCCGGCACCTGGTGTTATTTACGTTAGTGTTGATCAGGCTTTTTTTACAA‑CTGTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg < 1:334223/150‑1 (MQ=255) tttttATCTTTCAGGTACCGGTGGATGTTTATCGGCAACTGGGGTAATTTACGTTAGTGGTGATCAGGTTTTTTTTACAA‑CTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGa < 2:553576/144‑1 (MQ=255) ttttATCGTTCAGGTACCGGGGGTTGGTTATCGGCACCTGGGGTGTTTTACGTTAGTGGTGATCGGGTTTTTTTTAAAA‑CTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg < 2:746957/148‑1 (MQ=255) tGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc > 1:773255/1‑151 (MQ=255) ttATCTTTCAGGTACCGGGGGATGGTTATCGGCAACTGGGGTGTTTTACTTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:54957/149‑1 (MQ=255) tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg > 2:548607/1‑150 (MQ=255) ttggggTACCCGGGGTTGTTTAGCGCCACCGGGGGTGATTTAGGTTGGTGGTGATCAGGTTTTTTTTACAT‑CTGTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt < 2:856426/144‑1 (MQ=255) tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt > 2:425879/1‑148 (MQ=255) cAGGGACCCGTGGAGGTTTATCGGCATCTGGGGTGATTTACGTTAGTGGAGATCGGGTTTTTTTAACAA‑TTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTaa < 1:693826/149‑1 (MQ=255) tGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTgga > 1:256338/1‑147 (MQ=255) ttctCGGAAACGGGGTTGATTTACGTTAGGGGTGAGCAGTTTTTTTTAACAA‑CTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat < 1:676098/147‑1 (MQ=255) ttATGGGCACCTGGGGTGTTTTACTTTAGTGGTGATCAGGTTTTTTTTACAA‑CTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa < 2:151678/149‑1 (MQ=255) aTCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattat > 1:386441/1‑151 (MQ=255) tCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt > 2:373926/1‑151 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc > 2:415446/1‑149 (MQ=255) cGGGTGATTTACTTTAGGGGTGATCAGGTTTTTTTTACAA‑CTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt < 1:499822/150‑1 (MQ=255) gggTGATTTACGTTAGTGGTGATCGGGTTTTTTTAACAT‑CTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca < 1:534794/146‑1 (MQ=255) gTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc > 2:865208/1‑149 (MQ=255) gATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg > 2:95492/1‑150 (MQ=255) aCGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt > 2:837774/1‑151 (MQ=255) ttAGTGGTGATCAGTTTTTTTTTACAA‑TTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa < 2:191007/149‑1 (MQ=255) tAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAcc > 2:577749/1‑150 (MQ=255) ggggtaATCAGGTTTTTTTTACAA‑CTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt < 2:73506/149‑1 (MQ=255) tggtgATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt > 1:891715/1‑151 (MQ=255) gttgatcggtttttttttCAATCTGTGTAAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt < 1:95472/140‑1 (MQ=255) gATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc > 1:808794/1‑150 (MQ=255) ttgaGGTTTTTTTTACAC‑TTTTTTGGAAAGAAAAAAGAAGACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt < 2:627799/143‑1 (MQ=255) acgagtttttttttCCAC‑CTGTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc < 2:407500/144‑1 (MQ=255) aGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc > 1:60830/1‑146 (MQ=255) cTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg > 1:603581/1‑151 (MQ=255) tttttttACAA‑TTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCg < 2:461242/143‑1 (MQ=255) ttttACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCCGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt > 1:898527/1‑151 (MQ=255) tttACAA‑CTTTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt < 2:122245/150‑1 (MQ=255) tttACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt < 1:884424/150‑1 (MQ=255) ttACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGGTGAAAGATTATTCAGCAGGTGCCGGCATTTTACCTTTTGCCGCTGGATGGTCt > 2:561148/1‑149 (MQ=255) aa‑CTTTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa < 1:22308/151‑1 (MQ=255) aa‑CTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg < 2:736355/150‑1 (MQ=255) tttGAGAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg < 2:87632/148‑1 (MQ=255) aagaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc < 1:621742/148‑1 (MQ=255) aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa < 1:44551/151‑1 (MQ=255) agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTTTCAGGAGCTtctcca > 1:388706/1‑147 (MQ=255) | GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA > NC_000913/1824909‑1825202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |