Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 64,352 G→C R477G (CGC→GGC)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091364,3520GC98.2% 198.7 / ‑3.1 54R477G (CGC→GGC) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (23/30);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

GATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGAT  >  NC_000913/64208‑64499
                                                                                                                                                |                                                                                                                                                   
gatgaCCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCGGGCGGTGGGGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTg                                                                                                                                               <  2:60/151‑1 (MQ=255)
      cACGCAGGGTGACGGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCa                                                                                                                                            <  1:62927/148‑1 (MQ=255)
       aCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATg                                                                                                                                        >  2:28410/1‑151 (MQ=255)
        cGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATg                                                                                                                                        >  1:287773/1‑150 (MQ=255)
           aTGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTa                                                                                                                                     <  2:371011/150‑1 (MQ=255)
            tGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAg                                                                                                                                    <  1:57375/150‑1 (MQ=255)
             ggTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGGGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTc                                                                                                                                  <  1:718345/151‑1 (MQ=255)
                 aTCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTcaca                                                                                                                               <  2:471157/150‑1 (MQ=255)
                     acgaTGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGAAAGCGGTTTGATACCCTGGCGTTTGGCTTCATCGCCCCCGTGACAGATGTTAGTCACAAtctc                                                                                                                          >  2:581397/1‑151 (MQ=255)
                      cgaTGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTcc                                                                                                                         >  1:656183/1‑151 (MQ=255)
                       gaTGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCg                                                                                                                        >  2:270431/1‑151 (MQ=255)
                       gaTGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCg                                                                                                                        >  1:413281/1‑151 (MQ=255)
                       gaTGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCg                                                                                                                        <  1:74021/151‑1 (MQ=255)
                          gCCAGCCGCGGATGGAAGAAGCGGCGGGCGGTGGGGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCggc                                                                                                                      <  1:199221/150‑1 (MQ=255)
                            cAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCggc                                                                                                                      <  1:609821/148‑1 (MQ=255)
                                 gcgGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATg                                                                                                              <  2:628657/151‑1 (MQ=255)
                                 gcgGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATg                                                                                                              <  1:390515/151‑1 (MQ=255)
                                     aTCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGtttt                                                                                                          >  1:401537/1‑151 (MQ=255)
                                     aTCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGtttt                                                                                                          <  1:576283/151‑1 (MQ=255)
                                       cGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGttttt                                                                                                         <  2:97597/150‑1 (MQ=255)
                                        gaagaaGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTtct                                                                                                       >  2:678982/1‑151 (MQ=255)
                                               cGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTcgcg                                                                                                   >  2:681833/1‑148 (MQ=255)
                                               cGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCgaga                                                                                                <  2:760752/151‑1 (MQ=255)
                                                  cAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAAcc                                                                                             <  2:828062/151‑1 (MQ=255)
                                                    ggCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTTGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAAcc                                                                                             >  2:206131/1‑149 (MQ=255)
                                                     gCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAgg                                                                                          <  2:663333/151‑1 (MQ=255)
                                                      cGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGc                                                                                         >  2:150076/1‑151 (MQ=255)
                                                          ggtgCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCg                                                                                     <  1:844250/151‑1 (MQ=255)
                                                             gCCGAGCACGCCATAAAAGTCATTCATGATGATTTTCAGCGCCTGCGGCAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCgaga                                                                                  <  1:412403/151‑1 (MQ=255)
                                                                      gCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCg                                                                         >  2:232634/1‑151 (MQ=255)
                                                                         aTAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTa                                                                      <  2:542711/151‑1 (MQ=255)
                                                                            aaaGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACtg                                                                   >  2:280690/1‑151 (MQ=255)
                                                                                  aTTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGctct                                                             >  2:890950/1‑151 (MQ=255)
                                                                                     cATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTgg                                                           >  2:797041/1‑150 (MQ=255)
                                                                                     cATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGa                                                          <  1:362012/151‑1 (MQ=255)
                                                                                      atgatgATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGAt                                                         >  1:138874/1‑151 (MQ=255)
                                                                                        gatgatTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATc                                                        <  2:268630/150‑1 (MQ=255)
                                                                                              tttCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTg                                                  <  2:59151/150‑1 (MQ=255)
                                                                                               ttCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTgcg                                                >  2:193947/1‑151 (MQ=255)
                                                                                                 cAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGcc                                              <  1:377075/151‑1 (MQ=255)
                                                                                                    cgcCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATg                                           >  2:192035/1‑151 (MQ=255)
                                                                                                     gcCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGc                                          >  2:605516/1‑151 (MQ=255)
                                                                                                       cTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCt                                        >  1:68203/1‑151 (MQ=255)
                                                                                                           gACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTcca                                    <  1:816285/151‑1 (MQ=255)
                                                                                                                  gTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGccc                             <  1:414260/151‑1 (MQ=255)
                                                                                                                           ccTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGatc                    <  2:296327/151‑1 (MQ=255)
                                                                                                                              ggCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGatca                   <  2:656358/149‑1 (MQ=255)
                                                                                                                                 gTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGatcaatc                >  2:252330/1‑149 (MQ=255)
                                                                                                                                 gTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAg              <  1:378703/151‑1 (MQ=255)
                                                                                                                                   ttGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGa             <  1:377772/150‑1 (MQ=255)
                                                                                                                                     ggCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAATCCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAg          <  1:123928/151‑1 (MQ=255)
                                                                                                                                     ggCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAg          <  1:539286/151‑1 (MQ=255)
                                                                                                                                       cTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGt        >  1:100194/1‑151 (MQ=255)
                                                                                                                                            tCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCgg    >  2:144327/1‑150 (MQ=255)
                                                                                                                                             cgccCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGgat  >  1:142306/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGAT  >  NC_000913/64208‑64499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑