breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 1,436,746 | T→C | K50E (AAA→GAA) | ompN ← | outer membrane pore protein N, non‑specific |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,141,832 | A→T | D128E (GAT→GAA) | dcd ← | deoxycytidine triphosphate deaminase; dCTP deaminase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,743,181 | A→G | I267V (ATC→GTC) | yfiN → | putative membrane‑anchored diguanylate cyclase |
RA | 3,440,924 | C→G | G36R (GGC→CGC) | rpoA ← | RNA polymerase, alpha subunit |
RA | 3,473,615 | C→T | W156* (TGG→TAG) | rpsG ← | 30S ribosomal subunit protein S7 |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,627,567 | C→T | Q85* (CAG→TAG) | nadR → | trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423781–3424234 | 3424527–3424238 | 5–747 | 16 [15] | [15] 16 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
* | * | ÷ | NC_000913 | 4392424 | 4392599 | 176 | 16 [15] | [15] 17 | [glyV]–[glyY] | [glyV], glyX, [glyY] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 2 (0.030) | 42 (0.710) | 39/266 | 0.6 | 96.8% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 1 (0.020) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 1 (0.020) | 47 (0.800) | 42/266 | 0.5 | 98.0% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 1 (0.020) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 47 (0.730) | 41/290 | 0.7 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3316548 = | 73 (1.100) | 85 (1.330) | 77/288 | 0.0 | 58.1% | coding (979/1488 nt) | nusA | transcription termination/antitermination L factor |
? | NC_000913 | = 3399212 | 52 (0.810) | coding (767/1104 nt) | mreC | cell wall structural complex MreBCD transmembrane component MreC |