Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 464,304 A→G 100% V181V (GTT→GTC)  queC ← 7‑cyano‑7‑deazaguanine synthase QueC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN832404464,3040AG97.3% 253.2 / ‑0.4 77V181V (GTT→GTC) queC7‑cyano‑7‑deazaguanine synthase QueC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (33/41);  minor base T (1/1);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTT  >  NZ_LN832404/464015‑464593
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 
gtttgtttGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTTCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTaa                                                                                                                                                                                                                                                                                              <  1:125485/295‑1 (MQ=255)
    gtttgtATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGAGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCAGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATca                                                                                                                                                                                                                                                                                          >  1:64743/1‑294 (MQ=255)
     tttgtATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCa                                                                                                                                                                                                                                                                                         >  1:447595/1‑295 (MQ=255)
         tATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGttt                                                                                                                                                                                                                                                                                     >  1:291492/1‑295 (MQ=255)
                 ttAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGtaat                                                                                                                                                                                                                                                                             <  1:891701/295‑1 (MQ=255)
                   aaaaCGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGtaataa                                                                                                                                                                                                                                                                           >  1:434233/1‑295 (MQ=255)
                     aaCGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCCTCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGTCGCGTAAATTACATGCCGCCCAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTCATAATc                                                                                                                                                                                                                                                                         >  1:683029/1‑295 (MQ=255)
                      aCGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACCATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGCCAACGTTTCGTTACGGACCACATCCCCTTTGCCGTaataattt                                                                                                                                                                                                                                                                        >  2:495053/1‑293 (MQ=255)
                        acAGAAAGGATGGGCGCGGGAAGGGAAAGTGCGCGAAAAGCGGGAGCAGAGGGTTAAGTAAACGTTAGAATGGGCAGGATGCTCAAGCCGGATCCGGCGACACCCGGATTAATAACCTCAACCCGGTTTTCTACTTCATCGCTGCCAGCACCGTCGGTTAATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGc                                                                                                                                                                                                                                                                      <  2:512134/294‑1 (MQ=255)
                           gAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAg                                                                                                                                                                                                                                                                   >  1:590654/1‑295 (MQ=255)
                            aaaGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAgc                                                                                                                                                                                                                                                                  >  1:391674/1‑295 (MQ=255)
                                       tctGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTc                                                                                                                                                                                                                                                       >  1:680191/1‑295 (MQ=255)
                                       tctGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTc                                                                                                                                                                                                                                                       <  1:712715/295‑1 (MQ=255)
                                       tctGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCAAGGTTTc                                                                                                                                                                                                                                                       >  1:823937/1‑295 (MQ=255)
                                           gAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCACAGGTTTCCGCt                                                                                                                                                                                                                                                   >  1:565874/1‑295 (MQ=255)
                                           gAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATCACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAAACGTTGGCGCGTAAATTACATGCCGCACAATGAACGAAACCGTCGCCCTTAAAGCCGTTATAGCCGGTCACCGTTTCGTTATGGAATATATACCGTTCATCGTAAGTATCTGACTCAGCCCAGGCTTACGCt                                                                                                                                                                                                                                                   >  2:600971/1‑295 (MQ=255)
                                            aaGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCtt                                                                                                                                                                                                                                                  >  2:276331/1‑295 (MQ=255)
                                            aaGGGGAATTGCGAGAAAAGCTGGGGCAGATGGTTAAGTAACCGTTTTCTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCtt                                                                                                                                                                                                                                                  <  1:203929/295‑1 (MQ=255)
                                              ggggAATTGTGCGAAAAGTTGGAGTAGATGGTTAAGTAAACGTTTGGTGGTGCCGGATGCTCAATCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTa                                                                                                                                                                                                                                                <  1:64870/295‑1 (MQ=255)
                                                  aaTTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTACATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCaa                                                                                                                                                                                                                                            >  1:390308/1‑295 (MQ=255)
                                                    ttggacgAAAAGCTGGAGCGGATGGTTAAGTAAACGTGTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATc                                                                                                                                                                                                                                          <  2:326919/290‑1 (MQ=255)
                                                     tGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGACTAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGTCATCACCGTCGGTCTATCGGCCAGATAACGATTCAAACCGTGGGCGCGTAAATTACACCCCGCACAATGACCGCCACCGTCGCCTTTTAAGCTGTTATAGACGGTCAACGTCTCCGTCCGGACCAAATCCCGTTTGCACTAATTCTTCGCCATCCCCCAGGCATGCCCATTATCaaccg                                                                                                                                                                                                                                         >  2:648134/1‑292 (MQ=255)
                                                        gcgAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTAGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCCTTATCAATCcaca                                                                                                                                                                                                                                      >  1:499005/1‑295 (MQ=255)
                                                        gcgAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCGCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCcaca                                                                                                                                                                                                                                      <  1:572004/295‑1 (MQ=255)
                                                         cggaaaTCTGGAGCAGATGTGTAAGTAACCGTATTGTGGTGCCGGATGCTCAAGCCGCGTCCGGCGGCACCCGGAATAATTACCGCAACCCGGTTTTCTGCTTCATCGCGGCCAGCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAAGCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACAt                                                                                                                                                                                                                                     <  1:869118/292‑1 (MQ=255)
                                                             agggtGGAGCAGATGGTTAAGTAAACGTTGTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAgc                                                                                                                                                                                                                                 <  2:243113/291‑1 (MQ=255)
                                                               gCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGGCCCCAGGTTACCGCTTTTCCAATCCACATACgcgg                                                                                                                                                                                                                               >  2:235188/1‑295 (MQ=255)
                                                                 tgggtccgATGGTTACGTAAACGTTTGGGGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAgcggcg                                                                                                                                                                                                                             <  2:139499/288‑1 (MQ=255)
                                                                       aGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCAGTTATAGAAGGTCAACGTCTCGCTACGGACTAAATCCACTTTGCCGTAATAAGCCGACAGCGCCCAGGACTCCGCTTTCCCACTCCACCCCCGAGCCGTTTCaa                                                                                                                                                                                                                       >  2:362719/1‑295 (MQ=255)
                                                                         aTGGTTAAGGAAACGTTAGGTGGGGCCGGATGCTCAAGCCGCAGCCGGCGACGCCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCaaaa                                                                                                                                                                                                                     <  1:96497/295‑1 (MQ=255)
                                                                               aaGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAata                                                                                                                                                                                                               <  1:387018/295‑1 (MQ=255)
                                                                                  tAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCt                                                                                                                                                                                                            <  1:747075/295‑1 (MQ=255)
                                                                                    aaCGTTGTTGGGTGCCGTATGCCCTAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCttt                                                                                                                                                                                                          <  2:590654/295‑1 (MQ=255)
                                                                                    aaCGGTTTGTGGTGCCTGATGCTCAAGCCGCATCCGGCGACCCCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCttt                                                                                                                                                                                                          <  1:173528/295‑1 (MQ=255)
                                                                                         gaggtggtgCCGGATGCACAAGCAGGATCCGGCGACAACCGGAATAATGTCCTCAACCCGGTGTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAAACGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACGAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCa                                                                                                                                                                                                     <  1:641306/292‑1 (MQ=255)
                                                                                                      aTGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGAc                                                                                                                                                                                        <  1:56223/295‑1 (MQ=255)
                                                                                                         cTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGc                                                                                                                                                                                     >  1:127042/1‑295 (MQ=255)
                                                                                                            aaGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACg                                                                                                                                                                                       <  1:533234/290‑1 (MQ=255)
                                                                                                            aaGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACg                                                                                                                                                                                       >  2:533234/1‑290 (MQ=255)
                                                                                                            aaGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTGTCTTCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATg                                                                                                                                                                                  <  2:699723/295‑1 (MQ=255)
                                                                                                                       ggcgaacccagcaataagTACCCCAACCCGGTTGTCTGCTTCATCCATGCCAGCACCGACGTTGTATCGGCCAGCTGATGATTCAAAGCGTTGGCGCGGAAATTAGATGCTGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAGCGTGTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCtt                                                                                                                                                                       <  2:198731/283‑1 (MQ=255)
                                                                                                                         agACACCCGGAATAAGTACCTAAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGAACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTc                                                                                                                                                                     <  1:213266/294‑1 (MQ=255)
                                                                                                                          gACACCCGGAATAAGTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTca                                                                                                                                                                    <  1:342629/295‑1 (MQ=255)
                                                                                                                           acacCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGATTAGTGCTTTcac                                                                                                                                                                   >  1:306435/1‑295 (MQ=255)
                                                                                                                              cccGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACaaa                                                                                                                                                                >  1:298013/1‑295 (MQ=255)
                                                                                                                                  gAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATCTCTTTCGCCATGCCCAAACTGACGGCCTGGTTTAGTGCTTTCCCAAACTct                                                                                                                                                            >  2:407958/1‑294 (MQ=255)
                                                                                                                                   aataatTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCTAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCAt                                                                                                                                                           <  1:317445/295‑1 (MQ=255)
                                                                                                                                     taatTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCACACCGTTGGCGCGTAAATTACATGCCTCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTCTAGCAGGTCAACGTTTCGTTCCGCACTAAATCCAATTTGCCTTAATAATCTGCACGCGCCCAGTTTTCCGCCTTATCCATCCACCACCGCAGCCTTTTCACATGAATATCTTTCCCCCGCCCCAAACTGACGGCCTGGCTTAGGTCTTCCACCAACTCCTcg                                                                                                                                                         >  2:867411/1‑295 (MQ=255)
                                                                                                                                         tACCTCAACCCGGTTTGCTGCTGCATCGATGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGc                                                                                                                                                     <  1:183240/295‑1 (MQ=255)
                                                                                                                                                       tttCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCg                                                                                                                                       >  1:767843/1‑295 (MQ=255)
                                                                                                                                                        ttCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCACTCCACATCAGCGGCGTTTCAAACCGAATATCTTTCGCCATGCCCAAACTGCCGGCATGGTTTAGTGCTTTCACAAACTCATCCCGGCACACCGGGTGCCCgg                                                                                                                                      >  2:260876/1‑295 (MQ=255)
                                                                                                                                                                  tCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGt                                                                                                                            <  1:276331/295‑1 (MQ=255)
                                                                                                                                                                     cttgccgtaCCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTTGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTc                                                                                                                         <  2:710511/287‑1 (MQ=255)
                                                                                                                                                                       gCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGc                                                                                                                       <  1:837785/295‑1 (MQ=255)
                                                                                                                                                                       gCCATCACCGTCGGATTATGGGCCAGATAAAGATGCAAACCGTTGGAGCGTAAATTACATGCCGCACAAGTACCGCGACCGTCGCCTGTAAAGCCGTAATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTACCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAAGATCTTTCGCCATGCCCAAACTGACGGCATGGTTTTGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGATTCGc                                                                                                                       <  2:141106/295‑1 (MQ=255)
                                                                                                                                                                         cATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAg                                                                                                                     >  1:167010/1‑295 (MQ=255)
                                                                                                                                                                         cATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTCCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACCCATCGCGGCAATCCGGGTACCCGGAGAAACCCGTTTCGCAg                                                                                                                     >  2:184019/1‑295 (MQ=255)
                                                                                                                                                                           tCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATCACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGATTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGCTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAc                                                                                                                   >  1:327705/1‑295 (MQ=255)
                                                                                                                                                                                gTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCCATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAg                                                                                                              >  1:609445/1‑295 (MQ=255)
                                                                                                                                                                                      ttATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa                                                                                                        >  2:870746/1‑295 (MQ=255)
                                                                                                                                                                                      ttATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa                                                                                                        <  1:513034/295‑1 (MQ=255)
                                                                                                                                                                                      ttATCGGACAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa                                                                                                        <  1:495053/295‑1 (MQ=255)
                                                                                                                                                                                          cGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTACTAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCCCAAACTCATCGCGGCAATCCGGGTAGCACGTGAACTCCGTTTCGCCGACCCCAGTAATTCCGGc                                                                                                    >  2:472311/1‑295 (MQ=255)
                                                                                                                                                                                                       aTTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCAAGGTTTAGTGCTTTCCCAAACTCATCGCGGCAATCCGGGGACCCGGAGAAACCCGTTTCCACGACGCCGGTAACTCAGGCCTCTGCTGTTccc                                                                                       >  2:546120/1‑295 (MQ=255)
                                                                                                                                                                                                             aCCGTTGGGGCGTAAATTCCAGGCCAGACAATGACCGCAACCGTCGCATTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATAc                                                                                 <  2:244823/295‑1 (MQ=255)
                                                                                                                                                                                                                             ttACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCa                                                                 <  1:145482/295‑1 (MQ=255)
                                                                                                                                                                                                                              tACATGCCGCCCAAGGACCGAAACCTTCTCCTTTAAGACCGTTATCTCAGTTCAACGTTTCGCTACCGCCTAAATCCATTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTGTCAATCCACATCCGCGGCGTTTCAAAACTAATATCTTTCGCCATGCCCTAACTTGCGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAg                                                                <  2:499005/295‑1 (MQ=255)
                                                                                                                                                                                                                              tACATGCCGCACAATTACCGCAACCGTCTCCTTTAAAGCCGTTATTGCAGGTCAACGTTTCGTTACGGACTAAATCCGGTTTGCCGTAAGAATCTGCCAGCGCCCAAGTTTCCGCTTTATCAATCCTGATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCGAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGTGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATCACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAg                                                                <  2:112190/295‑1 (MQ=255)
                                                                                                                                                                                                                                cagcgcGCAAAATGACGTCAACCGGCGCCGTTAAAGCCGTGATAGCAGGTAAACGTTTCGTTACGGACTAAAGCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCg                                                              <  2:680191/290‑1 (MQ=255)
                                                                                                                                                                                                                                  tGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTc                                                            >  1:354110/1‑295 (MQ=255)
                                                                                                                                                                                                                                       caaacgtaCCGCAACCAGCGCCTTTGACGCCGTTATAGCAGGTCAACGTTTCGTTACTGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACTAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGaa                                                       <  2:574355/288‑1 (MQ=255)
                                                                                                                                                                                                                                         caATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAAGCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACa                                                     <  2:127042/295‑1 (MQ=255)
                                                                                                                                                                                                                                             gACCGCAACCGTAGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAAGCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTGTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAat                                                 <  1:670612/295‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaaCGGTCGCCTTTTAAGGCGTTATCGCGTGTCAACGTTGCGTGACGGACCAAATCCAGTTTGCCGTAATAATCTGCCATCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGATTATCTTTCGCCATGCCCACACCGGCGGCATAGTTTAGTGCCTTCACAAACGCATCGCGGCAATCCGGGTAGCCGGAGAAATGCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGCTACGCATATATTGCCGCCAGCGTCAGTAACAAAATATTAc                                            <  2:434233/294‑1 (MQ=255)
                                                                                                                                                                                                                                                       gTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCt                                       >  1:855331/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                  aGCCGGTAGAGAAGGTCAACGGTTCGGTACGGACTAAATCCAGTGTGCCGTAATAATCAGCCAGCGCCCAGGTTTCCGCGTTAGCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGt                            <  2:167010/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACgaa                           <  1:561050/295‑3 (MQ=255)
                                                                                                                                                                                                                                                                      gTTATAGCAGGTCAACGTTTCGTTACGTACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACACACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTCATTACGGCTTCTGCTTTTACCTGATCCGCATATATTGCAGCCAGCGTCCGGAACACACTATTACGCCCTGGGCCAACCGTCTTc                        >  2:358129/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                       ttATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATCCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCCCGGCAATCCCGGGAGACGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCGTCTGCTTGTACCTCATACGAGTATAGTGCCCCCAGCGTCCGGACCAATATATTACGCCCTGGTACAAACGTATTCg                       >  2:673477/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                        tataGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCgg                      >  2:617340/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                        tataGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCgg                      <  1:235188/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ataGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACACACGTATTCggg                     >  1:520003/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                            gCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATa                  >  1:761556/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                               cgTCAACGGTTCGTTACTGACTAAATCCTGGTGGCCATAAGATTCGGCCAGCGCCCAGGTTTCCGCGTTATCCATCCACATCAGCGGCTTTTCATAACGAATAGCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCa               <  2:64743/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCAt              <  1:118591/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         tCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAACATATTACGCCCTGGGACAAACGTATTCGGGCTACCATCGGCTTCAg     >  1:160702/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                            tgaCGTACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGtt  <  1:358129/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 
GTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGGAACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTT  >  NZ_LN832404/464015‑464593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑