Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 1,154,267 2 bp→CC 100% coding (865‑866/2544 nt) mdoH → glucans biosynthesis glucosyltransferase MdoH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324041,154,2670TC100.0% 246.1 / ‑6.7 69L289P (TTG→CCG) mdoHglucans biosynthesis glucosyltransferase MdoH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (33/35);  minor base A (0/1);  total (33/36)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*NZ_LN8324041,154,2680TC98.7% 239.5 / ‑5.8 69L289P (TTG→CCG) mdoHglucans biosynthesis glucosyltransferase MdoH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (31/36);  minor base A (2/0);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATAC  >  NZ_LN832404/1153981‑1154550
                                                                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                                                          
tATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGGCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATc                                                                                                                                                                                                                                                                                     <  1:258435/295‑1 (MQ=255)
    cTGGTCCTCTTGGGGGTACGGTTCTGTTGGGTGTCCGGCGGATTCTGGACGGCGGTAATGGGCGTCCGGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCg                                                                                                                                                                                                                                                                                 <  2:774201/295‑1 (MQ=255)
       aTCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCAAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATCTCTGCGTCg                                                                                                                                                                                                                                                                              >  1:182613/1‑295 (MQ=255)
          ctctTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCag                                                                                                                                                                                                                                                                           >  2:148132/1‑295 (MQ=255)
          ctctTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGAGTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGTCGGTGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCag                                                                                                                                                                                                                                                                           <  1:705437/295‑1 (MQ=255)
             tttGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGc                                                                                                                                                                                                                                                                        <  1:398971/295‑1 (MQ=255)
                     acggttctgttGGGTGTCCGCCGGAGTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGGGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGcc                                                                                                                                                                                                                                                                <  1:708151/292‑1 (MQ=255)
                         ttctgttGGGTGTCAGCCGGAGTCTGGACGTAGTTAATGGGCTTCCTGCAACGGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGATGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGAAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCtgga                                                                                                                                                                                                                                                            <  1:525105/295‑1 (MQ=255)
                               tGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGc                                                                                                                                                                                                                                                      <  1:699879/295‑1 (MQ=255)
                                 ggTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCtt                                                                                                                                                                                                                                                    <  1:253914/295‑1 (MQ=255)
                                 ggTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCtt                                                                                                                                                                                                                                                    <  1:851598/295‑1 (MQ=255)
                                   tgtCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGAGGGAGCTTAt                                                                                                                                                                                                                                                  >  1:554452/1‑295 (MQ=255)
                                            gATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCAGGAAATCTGCGTCGCAGAGAAAAAAGCCTGGCTGGAGCTTATCGCTGACGt                                                                                                                                                                                                                                         >  2:82187/1‑295 (MQ=255)
                                                cTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGt                                                                                                                                                                                                                                     <  1:781499/295‑1 (MQ=255)
                                                    aCGGCGTTAATGGGATTCTTGGAACTGGTTAGTGGGCGCGGTAAATACAGGATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTAGCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCg                                                                                                                                                                                                                                 <  2:47675/295‑1 (MQ=255)
                                                     cGGCGTTAGTGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGa                                                                                                                                                                                                                                <  1:829548/295‑1 (MQ=255)
                                                      actgtccgatctcTTCCTGCAATTGCTTATTGGTCGCGATAAATACAGGAGATCTGCGTCAACAGTTGGCGATGAACCATGAAACCCGGAGCATCGCACGGCGTGGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGaa                                                                                                                                                                                                                               <  2:404530/283‑1 (MQ=255)
                                                           tAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCGGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCa                                                                                                                                                                                                                          <  2:401513/295‑1 (MQ=255)
                                                             aTGGGCTTCTGGCAACTGCTTATTGGTCGCGATAAAGACAGTATATCTGCGTCAACAGTTGGCGAGGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGa                                                                                                                                                                                                                        <  2:677642/295‑1 (MQ=255)
                                                                   ttCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAgagctcggaagag                                                                                                                                                                                                                  >  1:404530/1‑283 (MQ=255)
                                                                     ccggCAACTGTTTTTTGGTACAGATAATATCAGTAGATCGGCGTAAACAGTTGGCGATGATCCATTAAACCCGGGGCAGCGCACGGCGTTGATCATGCCTATCGGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGAGGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTAt                                                                                                                                                                                                                <  2:666645/292‑1 (MQ=255)
                                                                       tGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATcg                                                                                                                                                                                                              >  1:450354/1‑295 (MQ=255)
                                                                           aCTGCGTAATGGTCGCGAGAAATACAGTATAGCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATcgccgc                                                                                                                                                                                                          <  2:579327/295‑1 (MQ=255)
                                                                              gCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATcgccgccgc                                                                                                                                                                                                       >  1:888034/1‑295 (MQ=255)
                                                                                 tATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGCATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGACGTCGGTGGCGAAGGTCCGCTTTTATATCGCCGCCGCCGt                                                                                                                                                                                                    >  1:586543/1‑295 (MQ=255)
                                                                                  aTTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAAAACTTTGATGTCTACATTATTAGTGACAGTTATAACCAGGATATATGAGTCGAAGAGCAAAAAGCATGGATGGAGCTTATAGATGAAGTCGGTGGAGAAGGTAAGATTTTCTCTCgcagtcgcagtc                                                                                                                                                                                                   >  1:656925/1‑295 (MQ=255)
                                                                                           gATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGccgccgccg                                                                                                                                                                                                     <  1:657281/284‑1 (MQ=255)
                                                                                           gATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGccgccgccg                                                                                                                                                                                                     >  2:657281/1‑284 (MQ=255)
                                                                                                 tACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTcgccgcagatcggcag                                                                                                                                                                                    >  1:564360/1‑285 (MQ=255)
                                                                                                   cAGGATAGCTGGGTCAAAAGGTGGCGATGAACCATTAAACCCGGAGCATCGGACGGCGTTGATCAGTCCTATCTGTAACGAAGACGTGAACCGTGTTTGTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTaaa                                                                                                                                                                                  <  2:764441/295‑1 (MQ=255)
                                                                                                     gTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAg                                                                                                                                                                                >  1:159060/1‑295 (MQ=255)
                                                                                                      tataTCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGc                                                                                                                                                                               >  1:413160/1‑295 (MQ=255)
                                                                                                       gcatctgggagaaCAGTTGGGGGTGAACCATTAAACCCGGAGCATTGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCg                                                                                                                                                                              <  2:791337/284‑1 (MQ=255)
                                                                                                          tCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACCTTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCCGAGCACAAAGCCTGGATGGAGCTTATAGATGAACTCGGTGGGCACGGGCAGATTTTCTATCTCCCGCGCCGCCGCGGCGGCAAGCGGAACAGCGGtg                                                                                                                                                                           >  2:853911/1‑294 (MQ=255)
                                                                                                                        aggCGATGAACCAGTAAACCCGGAGCATCGCACGGGTTTAAACATGCTTATCTGTAACGAAGACATGAACCGTGTGTTTGCTGGCCTGCGTGCAACGTAGGAAGCAGTAAAAGCCACCGGGAAGGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTAGAACCCGGATATCTGCGGCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTc                                                                                                                                                             <  2:751452/294‑1 (MQ=255)
                                                                                                                          gCGATGGACCATTAAAACCGGAGCATCGCACGGCGTTGGTCATGCCTGGCGGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTg                                                                                                                                                           <  2:606679/295‑1 (MQ=255)
                                                                                                                             aTGAACCATTAAAACCGAGGAGTCGGACGGGGGTGATAATGCCGATCGTGAACGAAGAAGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGGGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCGGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCg                                                                                                                                                        <  2:857552/295‑1 (MQ=255)
                                                                                                                                  ccATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGGGAAGCGTAAAAGCGGTAATATCGATGACTTATGCCGTCGcg                                                                                                                                                   >  2:311398/1‑294 (MQ=255)
                                                                                                                                                gCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTAc                                                                                                                                     >  2:595446/1‑295 (MQ=255)
                                                                                                                                                     gCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAACGCGGTAATATCGATGACtt                                                                                                                                                              >  2:395936/1‑265 (MQ=255)
                                                                                                                                                     gCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACtt                                                                                                                                                              <  1:395936/265‑1 (MQ=255)
                                                                                                                                                     gCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTa                                                                                                                                <  1:595446/295‑1 (MQ=255)
                                                                                                                                                             ttGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTTTCGCCGCCGCCGTCACCGCGTGGAGCGTAAAAGCGGTAATGTCGCGTAACTCTGGCGTCGGTGGGGAAGCCAGTACCGCTACAgggtgg                                                                                                                        >  2:66363/1‑295 (MQ=255)
                                                                                                                                                              tGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCAGTCGCTGGGGCAGCCAGTACAGCTACATggtggt                                                                                                                       >  1:68661/1‑295 (MQ=255)
                                                                                                                                                               gatgatGCCTGTCGGTAAGGAAGACGGTAACCGTGTTTTTGCTGGCCGGCGTGGAACGTGGGAATCAGTAAAAGCCAACGGGAATGCCAAACACTTTGTTGTCTACATTCTTAGTGACAGTTAGAACCCAGATATCTGCGTCGCAGAGCAAAAGGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGGGAAGCGTAAAAGCGGTGATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGgtggtg                                                                                                                      <  2:85306/295‑1 (MQ=255)
                                                                                                                                                                    gtccTAGCTGTACCGAAGACGTGAACCGTGTTTTTGCTGGCATGCGTGCAACGGTGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTGTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGa                                                                                                                 <  2:519387/293‑1 (MQ=255)
                                                                                                                                                                       cTATCTGTAACGAAGACGTGAACCGTGTTGTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGc                                                                                                              <  1:98370/295‑1 (MQ=255)
                                                                                                                                                                            tGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCCGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACt                                                                                                         >  1:13091/1‑295 (MQ=255)
                                                                                                                                                                                aCGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGt                                                                                                     >  1:659763/1‑295 (MQ=255)
                                                                                                                                                                                   aaGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCTCCGCGTGAAGCGTAAAAGCGGTAATATCGTTGTCTTCTGCCGTCGCTGGGGCAGCAAGTACAGCTACATGTTGGTGCTGGATGCTGACTCGGTGAt                                                                                                  >  1:537269/1‑295 (MQ=255)
                                                                                                                                                                                        gTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCg                                                                                             >  1:505433/1‑295 (MQ=255)
                                                                                                                                                                                           aaCCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGTTGGCGAAGGTCAGATTTTCTATCGCCGCCTCCGTCGCCGCGTAAAGCGTAAAAACGGTAAATTCGATGAATTCTGCCGTCGCTGGGGCAGCCCTAACAGGTCTATGGCGGTGCGTGGTGCTGACTCgcttgaccgggatc                                                                                          >  2:186110/1‑282 (MQ=255)
                                                                                                                                                                                                   ttttGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGAttgtttg                                                                                  <  2:888034/295‑1 (MQ=255)
                                                                                                                                                                                                    tttGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGAACGGTGAgtgtttgt                                                                                 >  1:73819/1‑295 (MQ=255)
                                                                                                                                                                                                          ggcctggtggCAACGTGGGAATCAGTAAAAGCCACCCGGAAGGCCAATCACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATAGCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGc                                                                           <  2:450354/286‑1 (MQ=255)
                                                                                                                                                                                                             gcacggggaaCGTGGGAAGCAGTAAAAGCCACCGGGAATGACAAATACTTTGATGTACGCAGTCTTTGTGACAGGTAGAACCCGGGTATCTGGGGCGGAGGGGAAAAGGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAATGTCAGATTTTCTATCGCCGCCGCCGTGGCCGCGTGAAGCGTAAAAGCGGTAATAGCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGGCCGGGGATTGTTTGTGCGGGCTgg                                                                        <  2:373227/287‑1 (MQ=255)
                                                                                                                                                                                                             cTGCGTGCAACTTGGGAATCAGTAAAAGCCACCGGTAATTCCAAACACTTTGATGTCTACAGTCTTAGTGACAGTTATAACCCGGATATCTGCGGCTCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTgg                                                                        <  1:405188/295‑1 (MQ=255)
                                                                                                                                                                                                                cGTGCAACGGGGGAAACAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACACGAATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTgc                                                                     <  1:853911/295‑1 (MQ=255)
                                                                                                                                                                                                                               tCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCa                                                      >  1:867509/1‑295 (MQ=255)
                                                                                                                                                                                                                                cAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGAACGGGGATTGTTTGGGCGGGCTGGTGCGCCTGGTGGCAGCCaa                                                     >  2:699320/1‑295 (MQ=255)
                                                                                                                                                                                                                                      aaGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCCGCCAGTACCGCTACCAGGTGGTGCTGGATGCTGACTCGGTAATGCACGGGGATTCTGTGGGCGGGGTGGTGCGGCGGGTGGACGCACACCCgga                                               >  2:523647/1‑293 (MQ=255)
                                                                                                                                                                                                                                             cGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCggg                                        >  1:149801/1‑295 (MQ=255)
                                                                                                                                                                                                                                                  aTGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGCAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGTATGCTGACTCGGTCATGGACGGTTCTTCTTTGTTCGGGCTGGTGCTCCTTATGGACGCCGACCCGACCGCCGGGagtct                                   >  2:773788/1‑291 (MQ=255)
                                                                                                                                                                                                                                                      cAAACACGTTGATGTCTCAAGTCTTAGTGACAGTTAGAACCCGGATATCGGGGTCGCAGGGCAAAAAGCCGGGATGGAGCTTATCGCTGAAGGCGGTGGCGAAGGTCAGATTTTCGATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAg                               <  2:465786/295‑1 (MQ=255)
                                                                                                                                                                                                                                                             tttGATGTCTACATTCTTAATGACAGTTATAACCCGAATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTTGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGc                        <  1:497814/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                gATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGACGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGCAGCGTAAAAGCGGTAATATCGCTGACTTCTGCCGTCGCTCGGGCAGACATTACAGCTACCTGGTGGTGCTGGATGCTGCCTCGGTAATGCCCGGTTATTGTTTGTGCGGGCTGGTGCGCCTGATTGTAGCCAACCCGAACGTCGGTAGCATTCAttcgcatcacc                     >  2:786636/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                 aTGTCTACATTCGTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGaa                    <  1:449676/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ttCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAg                  <  1:311398/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aCATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTc              >  1:711276/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                            cTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTa         <  1:148132/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATTACTTCTGCCGTCGCCGGGGATGCCAGTACAGCAACATGGTGGTGCTGGCTGCCGACTCCGTACAGACCGGCGCCTGTTTGTGCGGGCTGTTGCGCCTGATGGAAGCCATCACGACGGCCAGGACCATTCCGTCGGCCCCCaaaccctcacgcatgga     >  2:518883/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                                   aCAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCCGTCGTCGCCGAAAGCGTCCGGCATGGGTAc  >  2:150776/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                                                          
TATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCCGGATTCTGGACGGCGTTAATGGGCTTCCTGCAACTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATAC  >  NZ_LN832404/1153981‑1154550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑