New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 4201782 =188 (1.240)17 (0.130) 17/240 13.5 9.6% intergenic (‑94/‑144) zraP/zraS Zn‑dependent periplasmic chaperone/sensory histidine kinase in two‑component regulatory system with ZraR
?NC_000913_3_KO_pgi 4201828 = 155 (1.170)intergenic (‑140/‑98) zraP/zraS Zn‑dependent periplasmic chaperone/sensory histidine kinase in two‑component regulatory system with ZraR
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 189 total aligned reads displayed.

TCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGT  >  NC_000913_3_KO_pgi/4201634‑4201931
                                                                                                                                                    |                                                                                                                                                     
   ccATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTa                                                                                                                                                   >  4:356633/1‑150 (MQ=255)
    cATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTaa                                                                                                                                                  <  3:393158/150‑1 (MQ=255)
        gCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAAt                                                                                                                                              <  1:512757/150‑1 (MQ=255)
        gCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAAt                                                                                                                                              <  3:399533/150‑1 (MQ=255)
           gAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGAc                                                                                                                                           >  3:145197/1‑150 (MQ=255)
            aaaGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACt                                                                                                                                          >  2:727739/1‑150 (MQ=255)
             aaGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGCAACGCTGATATGACCGGTAATCTGGCCTGATAGACGAGTAAAAATGtctc                                                                                                                                         <  2:1209942/150‑1 (MQ=255)
                 gcCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGGTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATTTGGCATGATAAACGAGTAAAAAAGACTCAccg                                                                                                                                     >  2:507137/1‑149 (MQ=255)
                    atcatTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCctgct                                                                                                                                  >  3:763332/1‑150 (MQ=255)
                       atTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCg                                                                                                                               >  3:853342/1‑150 (MQ=255)
                       atTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCg                                                                                                                               <  1:1382743/150‑1 (MQ=255)
                       atTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCAAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCg                                                                                                                               <  2:1302234/150‑1 (MQ=255)
                        ttACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCgg                                                                                                                              >  1:273405/1‑150 (MQ=255)
                        ttACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCgg                                                                                                                              <  1:54156/150‑1 (MQ=255)
                         tACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCggg                                                                                                                             >  2:1098/1‑150 (MQ=255)
                         tACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTGCCGTGCCAGCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCggg                                                                                                                             <  4:7897/150‑1 (MQ=255)
                           ccAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTa                                                                                                                           >  2:352513/1‑150 (MQ=255)
                           ccAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTa                                                                                                                           <  2:164034/150‑1 (MQ=255)
                           ccAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTa                                                                                                                           <  3:918043/150‑1 (MQ=255)
                            cAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAAt                                                                                                                                              >  1:919477/1‑130 (MQ=255)
                            cAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAg                                                                                                                          >  2:45217/1‑150 (MQ=255)
                             aGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGc                                                                                                                         <  4:48498/150‑1 (MQ=255)
                             aGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGc                                                                                                                         <  4:958058/150‑1 (MQ=255)
                             aGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGCGTAGc                                                                                                                         <  4:853342/150‑1 (MQ=255)
                               ggCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACt                                                                                                                                          >  4:840064/1‑131 (MQ=255)
                                gCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGa                                                                                                                                                      <  1:147923/118‑1 (MQ=255)
                                  aaTTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGGATTGCATCTTACGTGCCAACGTTTGAACGCTGGTATGAGTGGTATTCTTGGATTGTAAACGAGTAATTGTGACTCGCCTGCTGCGGGTAGCGAGTc                                                                                                                    >  1:159738/1‑150 (MQ=255)
                                    ttttCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCAAGATAACCGAGTCAAAAAAACACGCCCGCGGCGGGTAGCGAGTCAt                                                                                                                  >  3:36944/1‑150 (MQ=255)
                                     tttCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCAtt                                                                                                                 >  2:1282458/1‑150 (MQ=255)
                                      ttCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTa                                                                                                                                                   <  1:1098636/115‑1 (MQ=255)
                                          tgtTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTAc                                                                                                            <  1:582210/150‑1 (MQ=255)
                                          tgtTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTAc                                                                                                            <  3:632164/150‑1 (MQ=255)
                                               cGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGa                                                                                                                                            >  3:297019/1‑113 (MQ=255)
                                               cGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCAtt                                                                                                       >  1:300654/1‑150 (MQ=255)
                                                gTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCTACCCGCAGCAGGCGAGTCATTTTTACTCGttt                                                                                                      >  1:1495491/1‑149 (MQ=25)
                                                   tCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGaaa                                                                                                   <  2:785629/150‑1 (MQ=255)
                                                   tCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGaaa                                                                                                   >  1:1222786/1‑150 (MQ=255)
                                                       ggTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTa                                                                                               <  2:479751/150‑1 (MQ=255)
                                                        gTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTaa                                                                                              >  2:1432289/1‑150 (MQ=255)
                                                        gTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTaa                                                                                              <  2:1217012/150‑1 (MQ=255)
                                                        gTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCAGCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTaa                                                                                              <  2:1310426/150‑1 (MQ=255)
                                                         ttAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAg                                                                                             <  3:119764/150‑1 (MQ=255)
                                                              cctccAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCttt                                                                                        <  3:479455/150‑1 (MQ=255)
                                                              cctccAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCttt                                                                                        <  1:978227/150‑1 (MQ=255)
                                                                tccAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTtgt                                                                                      >  2:636585/1‑150 (MQ=255)
                                                                tccAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATAACTCACCTGCTGCGGGTAGCGAGTCGTTTTTACTCATTGAAACTTAAGCCtttat                                                                                      >  2:951641/1‑148 (MQ=255)
                                                                      ggTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAACTGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACa                                                                                <  1:489421/150‑1 (MQ=255)
                                                                       gTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAg                                                                               >  2:1441519/1‑150 (MQ=255)
                                                                           tCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCa                                                                           >  1:534483/1‑150 (MQ=255)
                                                                           tCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCa                                                                           <  2:534483/150‑1 (MQ=255)
                                                                             ttACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAgg                                                                         >  2:471241/1‑150 (MQ=255)
                                                                                cTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTaa                                                                                              >  1:624975/1‑126 (MQ=255)
                                                                                  tCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAg                                                                    <  3:1014608/150‑1 (MQ=255)
                                                                                  tCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAAACTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAg                                                                    >  1:1655992/1‑150 (MQ=255)
                                                                                     ggTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAgcgc                                                                 >  2:1671185/1‑150 (MQ=255)
                                                                                        aTTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGa                                                              <  1:152986/150‑1 (MQ=255)
                                                                                            cATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCAtttt                                                                                                               >  1:1294536/1‑97 (MQ=255)
                                                                                            cATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATaaa                                                          >  1:340181/1‑150 (MQ=255)
                                                                                            cATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATaaa                                                          >  2:793962/1‑150 (MQ=255)
                                                                                            cATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATaaa                                                          >  4:684117/1‑150 (MQ=255)
                                                                                             aTCTTCCGTGCCAGCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGTGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTGACAGCGCAGGGTAAGCGCTGATaaaa                                                         <  4:580450/150‑1 (MQ=255)
                                                                                             aTCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCAt                                                                                                        >  3:249234/1‑103 (MQ=255)
                                                                                             aTCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATaaaa                                                         >  3:820628/1‑150 (MQ=255)
                                                                                              tCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAg                                                        >  4:1037522/1‑150 (MQ=255)
                                                                                                ttCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAGACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGAt                                                      <  4:194085/150‑1 (MQ=255)
                                                                                                ttCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGAt                                                      >  2:624835/1‑150 (MQ=255)
                                                                                                tgccGTGCCAACGTTGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGAt                                                      <  2:655246/148‑1 (MQ=255)
                                                                                                 tCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATg                                                     <  4:331086/150‑1 (MQ=255)
                                                                                                  ccGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATgg                                                    <  3:848254/150‑1 (MQ=255)
                                                                                                      gCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATg                                                >  1:1156531/1‑150 (MQ=255)
                                                                                                        cAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGAt                                              <  1:616467/150‑1 (MQ=255)
                                                                                                        cAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGAt                                              >  3:801610/1‑150 (MQ=255)
                                                                                                        cAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGAt                                              >  2:885703/1‑150 (MQ=255)
                                                                                                         aaCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCgg                                                                                                                              >  3:349176/1‑69 (MQ=255)
                                                                                                         aaCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGc                                                                                            >  3:122248/1‑103 (MQ=255)
                                                                                                         aaCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGc                                                                                            <  4:122248/103‑1 (MQ=255)
                                                                                                         aaCGATGAAACGCTGATATGACGGGTAATCTGGCAGGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATCCAAGATGGAATGAtt                                             >  4:725764/1‑150 (MQ=255)
                                                                                                          aCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATgg                                                    <  2:1601974/142‑1 (MQ=255)
                                                                                                          aCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGAttt                                            >  2:1024540/1‑150 (MQ=255)
                                                                                                          aCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCAGATAAAAGATGGCATGAttt                                            >  2:410679/1‑150 (MQ=255)
                                                                                                            gATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTct                                          <  1:742185/150‑1 (MQ=255)
                                                                                                            gATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTct                                          >  4:279915/1‑150 (MQ=255)
                                                                                                            gATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTct                                          >  4:560286/1‑150 (MQ=255)
                                                                                                             aTGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGAt                                              <  4:814272/145‑1 (MQ=255)
                                                                                                             aTGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctg                                         <  1:45217/150‑1 (MQ=255)
                                                                                                              tGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctgc                                        <  3:52043/150‑1 (MQ=255)
                                                                                                                 aaCGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGt                                     <  2:1156531/150‑1 (MQ=255)
                                                                                                                  aCGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTc                                    <  2:368067/150‑1 (MQ=255)
                                                                                                                   cGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCa                                   <  2:1553001/150‑1 (MQ=255)
                                                                                                                         tatGAGGGGTAATCTGGCCTGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAgg                             <  1:507137/150‑1 (MQ=255)
                                                                                                                            gACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGAt                          <  2:300654/150‑1 (MQ=255)
                                                                                                                             aCGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATg                         <  1:1098/150‑1 (MQ=255)
                                                                                                                               gggTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCGTTTTTGTTATAGTGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAg                       >  3:465707/1‑150 (MQ=255)
                                                                                                                                 gTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCa                                   >  2:961677/1‑136 (MQ=255)
                                                                                                                                 gTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCa                                   <  1:961677/136‑1 (MQ=255)
                                                                                                                                  tAATATGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAg                    <  4:514626/150‑1 (MQ=255)
                                                                                                                                        ggCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaag              <  3:910047/150‑1 (MQ=255)
                                                                                                                                         gCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaaga             <  1:793962/150‑1 (MQ=255)
                                                                                                                                            tGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAaagaaga          <  1:1070580/150‑1 (MQ=255)
                                                                                                                                                    gAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGt  <  1:404389/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCGCCATTGCTGAAAGCGCCATCATTACCAGGGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGT  >  NC_000913_3_KO_pgi/4201634‑4201931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.