New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 3386201159 (1.050)17 (0.120) 15/252 15.2 10.3% intergenic (‑72/+20) yhcO/aaeB putative barnase inhibitor/p‑hydroxybenzoic acid efflux system component
?NC_000913_3_KO_pgi = 3386206 149 (1.070)intergenic (‑77/+15) yhcO/aaeB putative barnase inhibitor/p‑hydroxybenzoic acid efflux system component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

AGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCC  >  NC_000913_3_KO_pgi/3386057‑3386355
                                                                                                                                                     |                                                                                                                                                     
  gTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGc                                                                                                                                                     >  1:783807/1‑150 (MQ=255)
   tttGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCg                                                                                                                                                    >  1:1003807/1‑150 (MQ=255)
    ttGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGt                                                                                                                                                   >  1:124257/1‑150 (MQ=255)
       gCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCgg                                                                                                                                                >  1:1046222/1‑150 (MQ=255)
          aaaGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGttt                                                                                                                                             <  2:518015/150‑1 (MQ=255)
             gTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGTCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGa                                                                                                                                          <  1:988239/150‑1 (MQ=255)
             gTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGa                                                                                                                                          <  1:635986/150‑1 (MQ=255)
                aCGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGtttt                                                                                                                                            >  4:239677/1‑145 (MQ=255)
                        aaTCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCg                                                                                                                               <  3:745252/150‑1 (MQ=255)
                        aaTCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATAGCTAAGCAACGCCACGAAACTGCCGGCACTGTTATCGTCGGTTTGGGCTTAACTATCg                                                                                                                               >  1:750331/1‑150 (MQ=255)
                             tcctGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCCAAAAGCGTCGGtttttt                                                                                                                                          >  1:1266753/1‑132 (MQ=255)
                                tGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGc                                                                                                                       <  2:646127/150‑1 (MQ=255)
                                    tctcAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATc                                                                                                                                >  1:1112181/1‑137 (MQ=255)
                                    tctcAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGt                                                                                                                   <  1:1334075/150‑1 (MQ=255)
                                     ctcAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGtt                                                                                                                  <  4:631220/150‑1 (MQ=255)
                                      tcAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTg                                                                                                                 >  4:300533/1‑150 (MQ=255)
                                       cAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                                                >  1:1438191/1‑150 (MQ=255)
                                             catcaAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTa                                                                                                          <  3:880314/150‑1 (MQ=255)
                                              atcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTAt                                                                                                         >  2:1072042/1‑150 (MQ=255)
                                               tcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGa                                                                                                                <  3:229949/142‑1 (MQ=255)
                                               tcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATg                                                                                                        <  2:783807/150‑1 (MQ=255)
                                               tcaaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATg                                                                                                        >  2:40119/1‑150 (MQ=255)
                                                caaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATgg                                                                                                       >  1:566783/1‑150 (MQ=255)
                                                caaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATgg                                                                                                       >  1:146380/1‑150 (MQ=255)
                                                caaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATgg                                                                                                       >  1:268927/1‑150 (MQ=255)
                                                 aaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGa                                                                                                      <  3:937105/150‑1 (MQ=255)
                                                 aaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGa                                                                                                      <  3:683049/150‑1 (MQ=255)
                                                 aaaatcaaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGa                                                                                                      <  3:568733/150‑1 (MQ=255)
                                                       aaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATcc                                                                                                >  2:868702/1‑150 (MQ=255)
                                                       aaaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATcc                                                                                                >  1:1191484/1‑150 (MQ=255)
                                                        aaGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATccc                                                                                               >  4:144869/1‑150 (MQ=255)
                                                              taAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAg                                                                                         >  3:1026600/1‑150 (MQ=255)
                                                               aaaTATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGc                                                                                        <  2:1685676/150‑1 (MQ=255)
                                                                aaTATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATCGCCATGAAAAACCCGACGCTTTTGGCGTCGGTTTTGACTTATCTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGGCAGcc                                                                                       <  2:591136/150‑1 (MQ=255)
                                                                   atTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGAt                                                                                    >  4:591018/1‑150 (MQ=255)
                                                                       aTCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTAc                                                                                <  2:23119/150‑1 (MQ=255)
                                                                        tCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGc                                                                                        >  2:1334396/1‑141 (MQ=255)
                                                                        tCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGc                                                                                        <  1:1334396/141‑1 (MQ=255)
                                                                          tGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCgg                                                                             >  4:380509/1‑150 (MQ=255)
                                                                          tGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCgg                                                                             >  1:1575665/1‑150 (MQ=255)
                                                                          tGTTGCCACCGTCACGTTGCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGACAAAACCGACGCTTTTGTCGTCGATATTGACTTAACTATCGGCCAACGCATGTTGATACGTATGGAGCATCGCCGCAGGCCGATTTACCgg                                                                             >  2:903171/1‑150 (MQ=255)
                                                                             tGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGtt                                                                           >  3:385731/1‑149 (MQ=255)
                                                                              gCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTcc                                                                         >  3:510164/1‑150 (MQ=255)
                                                                                 aCCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTACCTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTc                                                                      <  4:737054/150‑1 (MQ=255)
                                                                                 aCCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTc                                                                      <  3:84644/150‑1 (MQ=255)
                                                                                     tCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCt                                                                  >  2:1469480/1‑150 (MQ=255)
                                                                                     tCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCt                                                                  >  2:329122/1‑150 (MQ=255)
                                                                                        cGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCg                                                               <  1:403306/150‑1 (MQ=255)
                                                                                         gTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACCTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCgg                                                              >  1:685547/1‑150 (MQ=255)
                                                                                            tCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTgg                                                           >  1:949428/1‑150 (MQ=255)
                                                                                             cgcgGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGgc                                                          <  3:300533/150‑1 (MQ=255)
                                                                                               cggcgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCg                                                               <  2:510860/143‑1 (MQ=255)
                                                                                               cggcgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCg                                                               >  1:510860/1‑143 (MQ=255)
                                                                                               cggcgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGgcgc                                                        >  4:457557/1‑150 (MQ=255)
                                                                                               cggcgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACAGGTTCCGTCACCTGCGGTGgcgc                                                        <  2:1441146/150‑1 (MQ=255)
                                                                                                 gcgAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCtt                                                      >  3:880549/1‑150 (MQ=255)
                                                                                                   gAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGc                                                    <  1:1531174/150‑1 (MQ=255)
                                                                                                     gTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTaa                                                                                                                                     <  1:139223/67‑1 (MQ=255)
                                                                                                     gTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTaa                                                                                                                                     >  2:139223/1‑67 (MQ=255)
                                                                                                      ttCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCa                                                                                                                           <  4:497254/76‑1 (MQ=255)
                                                                                                         tGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTAt                                                                                                                                 <  1:1244511/67‑1 (MQ=255)
                                                                                                         tGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTAt                                                                                                                                 >  2:1244511/1‑67 (MQ=255)
                                                                                                         tGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCg                                                               <  1:206080/133‑1 (MQ=255)
                                                                                                         tGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCg                                                               >  2:206080/1‑133 (MQ=255)
                                                                                                         tGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATg                                              >  2:1598660/1‑150 (MQ=255)
                                                                                                         tGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGGTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATg                                              >  2:959820/1‑150 (MQ=255)
                                                                                                         tGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACCTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATg                                              >  4:404813/1‑150 (MQ=255)
                                                                                                          gCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGGCAGATGc                                             <  4:880549/150‑1 (MQ=255)
                                                                                                           ccGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCg                                            <  3:282267/150‑1 (MQ=255)
                                                                                                             gTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTa                                          <  3:481471/150‑1 (MQ=255)
                                                                                                                 aTCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGc                                                                                        >  1:464055/1‑100 (MQ=255)
                                                                                                                  tCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCttt                                     <  4:1026600/150‑1 (MQ=255)
                                                                                                                    gCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTc                                   <  2:67037/150‑1 (MQ=255)
                                                                                                                     cTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGtt                                                                           <  4:798435/109‑1 (MQ=255)
                                                                                                                     cTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGtt                                                                           >  3:798435/1‑109 (MQ=255)
                                                                                                                      tAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCt                                 <  1:903171/150‑1 (MQ=255)
                                                                                                                        aGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTgg                               >  1:1003275/1‑150 (MQ=255)
                                                                                                                        aGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTgg                               <  4:558294/150‑1 (MQ=255)
                                                                                                                         gTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGt                              <  1:1661870/150‑1 (MQ=255)
                                                                                                                          tataGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa                             <  4:274211/150‑1 (MQ=255)
                                                                                                                          tataGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTa                             <  2:1575665/150‑1 (MQ=255)
                                                                                                                               ccATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGa                                                                                     >  2:122131/1‑89 (MQ=255)
                                                                                                                                 aTGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTcc                      <  2:566783/150‑1 (MQ=255)
                                                                                                                                 aTGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTcc                      <  2:268927/150‑1 (MQ=255)
                                                                                                                                 aTGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTcc                      >  1:727504/1‑150 (MQ=255)
                                                                                                                                  tGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCa                     <  1:218593/150‑1 (MQ=255)
                                                                                                                                  tGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCa                     <  2:1126820/150‑1 (MQ=255)
                                                                                                                                    tcaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCt                                 >  4:380223/3‑136 (MQ=255)
                                                                                                                                    aaaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGt                   >  3:158706/1‑150 (MQ=255)
                                                                                                                                     aaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGc                                                                                        >  2:1652352/1‑80 (MQ=255)
                                                                                                                                     aaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGgc                                                          <  1:344985/110‑1 (MQ=255)
                                                                                                                                     aaaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGgc                                                          >  2:344985/1‑110 (MQ=255)
                                                                                                                                      aaaaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGttc                 <  2:1249885/150‑1 (MQ=255)
                                                                                                                                        aaCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGttctt               <  2:109977/150‑1 (MQ=255)
                                                                                                                                         aCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGttcttc              <  3:33473/150‑1 (MQ=255)
                                                                                                                                         aCCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGttcttc              <  2:685547/150‑1 (MQ=255)
                                                                                                                                            gACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAg           <  3:380509/150‑1 (MQ=255)
                                                                                                                                                cTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAAc       <  2:983983/150‑1 (MQ=255)
                                                                                                                                                  tttGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATg                                                                                                        <  2:149739/51‑1 (MQ=255)
                                                                                                                                                     ggCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGcc  <  2:124257/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGTTTGGCTAAAGTCACGATAAAAATCCTCCTGACTCTCAATCTCATCAAAATCAAAGGTATAAATATTCATCTGTTGCCACCGTCACGTTTCGCGGCGAGTTCTGCCGTGCATCGCTAAGTATAGCCATGAAAAAACCGACGCTTTTGGCGTCGGTTTTGACTTAACTATCGGTCAACGCATGTTGATACTTATGGAGCATCCCCGCCAGCCGATTTACCGGTTCCGTCACCTGCGGTGGCGCTTGCCAGATGCGTAGCTTTTCCTGGTAGATTTCCAGTTCTTCCAGCAACTGGCC  >  NC_000913_3_KO_pgi/3386057‑3386355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.