Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	668,742	C→G	E99Q (GAA→CAA)	rsfS ←	ribosomal silencing factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	668,742	0	C	G	100.0%	168.8 / NA	47	E99Q (GAA→CAA)	rsfS	ribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (24/23);  total (24/23)

GCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGG  >  NC_000913_3_KO_pgi/668597‑668888                                                                                                                                                  |                                                                                                                                                   gccggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATAc                                                                                                                                                <  1:643583/150‑1 (MQ=255)  ccggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACa                                                                                                                                               <  1:262276/150‑1 (MQ=255)   cggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAg                                                                                                                                              <  2:93974/150‑1 (MQ=255)    ggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAgg                                                                                                                                             >  3:354103/1‑150 (MQ=255)     gAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGc                                                                                                                                            >  4:405853/1‑150 (MQ=255)                  cTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc                                                                                                                               <  4:354103/150‑1 (MQ=255)                    cGAAGGGCATATCTTTCGGAACACGACGCCGGTACTCGGTAACACCGGTTTGTACCCAGTCCGGCCTTTTCCTTCCCACGGCGACAAGTTGCATCTTCACTCATTAATTCCATACTTTTTCCAGTTGATACAGGCGACGTCTCTCTTCCt                                                                                                                             <  2:62827/150‑1 (MQ=255)                     gAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACa                                                                                                                                               >  2:621671/1‑131 (MQ=255)                     gAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACa                                                                                                                                               <  1:621671/131‑1 (MQ=255)                     gAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTg                                                                                                                            >  1:419652/1‑150 (MQ=255)                        gggCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt                                                                                                                         <  2:441873/150‑1 (MQ=255)                                cTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                                 <  3:112323/150‑1 (MQ=255)                                 tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGAc                                                                                                                >  3:189203/1‑150 (MQ=255)                                   tCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACaa                                                                                                              <  4:348602/150‑1 (MQ=255)                                      gAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACa                                                                                                                     >  2:17084/1‑140 (MQ=255)                                      gAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACa                                                                                                                     <  1:17084/140‑1 (MQ=255)                                        aaaCGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGAc                                                                                                                                         <  1:455857/118‑1 (MQ=255)                                        aaaCGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGAc                                                                                                                                         >  2:455857/1‑118 (MQ=255)                                                                 cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                                            >  1:451280/1‑138 (MQ=255)                                                                 cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                                            <  2:451280/138‑1 (MQ=255)                                                                   gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                                                                              >  2:524125/1‑150 (MQ=255)                                                                      tGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                                                                           <  3:405853/150‑1 (MQ=255)                                                                       gTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATcc                                                                                             >  4:302152/1‑131 (MQ=255)                                                                       gTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATcc                                                                                             <  3:302152/131‑1 (MQ=255)                                                                          cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCt                                                                                                                             >  2:604757/1‑96 (MQ=255)                                                                          cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCt                                                                                                                             <  1:604757/96‑1 (MQ=255)                                                                           ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATa                                                                                                                                                 >  2:225076/1‑75 (MQ=255)                                                                           ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATa                                                                                                                                                 <  1:225076/75‑1 (MQ=255)                                                                                  ggCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt                                                               >  2:217731/1‑150 (MQ=255)                                                                                   gCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCtt                                                              <  2:419652/150‑1 (MQ=255)                                                                                            ttCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGa                                                     >  4:263036/1‑150 (MQ=255)                                                                                                 aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGa                                                                                                                                          >  2:292229/1‑60 (MQ=255)                                                                                                 aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGa                                                                                                                                          <  1:292229/60‑1 (MQ=255)                                                                                                 aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGt                                                >  3:243856/1‑150 (MQ=255)                                                                                                 aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGt                                                <  4:243856/150‑1 (MQ=255)                                                                                                  cGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTa                                               >  2:545242/1‑150 (MQ=255)                                                                                                       aCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                                 >  3:127357/1‑79 (MQ=255)                                                                                                       aCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                                 <  4:127357/79‑1 (MQ=255)                                                                                                        cAAGTTGCAGCTTCACGCCTTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATGCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGCGCGGTAACAGGc                                         >  2:430257/1‑150 (MQ=255)                                                                                                              gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctctc                                                                                                                                  <  1:220850/55‑1 (MQ=255)                                                                                                              gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctctc                                                                                                                                  >  2:220850/1‑55 (MQ=255)                                                                                                                      aCGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGAc                           >  4:284332/1‑150 (MQ=255)                                                                                                                             aaCTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCATGca                    >  1:456795/1‑150 (MQ=255)                                                                                                                                   agagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACAGGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGt              >  2:415233/1‑150 (MQ=255)                                                                                                                                             cAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAAt    <  1:545242/150‑1 (MQ=255)                                                                                                                                              aGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATg   <  1:524125/150‑1 (MQ=255)                                                                                                                                               gTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGTTCAGCAATgg  >  2:415257/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGG  >  NC_000913_3_KO_pgi/668597‑668888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑