Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 1,166,727 G→C E215Q (GAA→CAA)  ndh → respiratory NADH dehydrogenase 2/cupric reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi1,166,7270GC97.1% 243.5 / ‑0.4 70E215Q (GAA→CAA) ndhrespiratory NADH dehydrogenase 2/cupric reductase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (31/37);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAA  >  NC_000913_3_KO_pgi/1166589‑1166864
                                                                                                                                          |                                                                                                                                         
ggCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGaa                                                                                                                                <  2:306398/150‑1 (MQ=255)
     aGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAt                                                                                                                           >  2:31193/1‑150 (MQ=255)
     aGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAt                                                                                                                           <  1:31193/150‑1 (MQ=255)
                  aTTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTa                                                                                                              >  4:291386/1‑150 (MQ=255)
                    tGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAcc                                                                                                            <  1:487084/150‑1 (MQ=255)
                       cggcggcggCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGcc                                                                                                         <  2:205647/150‑1 (MQ=255)
                       cggcggcggCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGcc                                                                                                         >  1:652550/1‑150 (MQ=255)
                         gcggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCAc                                                                                                       >  2:329094/1‑150 (MQ=255)
                         gcggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCAc                                                                                                       <  4:137024/150‑1 (MQ=255)
                         gcggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCAc                                                                                                       >  4:256105/1‑150 (MQ=255)
                                     cGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgctg                                                                                           <  4:30557/150‑1 (MQ=255)
                                      gggTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCgg                                                                                                                                     >  1:218998/1‑107 (MQ=255)
                                      gggTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCgg                                                                                                                                     <  2:218998/107‑1 (MQ=255)
                                         tgtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGc                                                                                       <  3:205478/150‑1 (MQ=255)
                                          gtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGcc                                                                                      <  3:291386/150‑1 (MQ=255)
                                            aGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCca                                                                                    <  3:256105/150‑1 (MQ=255)
                                            aGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCca                                                                                    <  3:243594/150‑1 (MQ=255)
                                                ctctCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAAc                                                                                <  1:604006/150‑1 (MQ=255)
                                                 tctcCGCTGCATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACg                                                                               >  1:472685/1‑150 (MQ=255)
                                                   tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAg                                                                             >  1:344564/1‑150 (MQ=255)
                                                       cTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctg                                                                                              >  1:60206/1‑129 (MQ=255)
                                                       cTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctg                                                                                              <  2:60206/129‑1 (MQ=255)
                                                       cTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTaa                                                                         <  2:472460/150‑1 (MQ=255)
                                                        tGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAAc                                                                        >  1:362680/1‑150 (MQ=255)
                                                        tGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAAc                                                                        <  4:322769/150‑1 (MQ=255)
                                                          aaTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGa                                                                      >  2:64686/1‑150 (MQ=255)
                                                             tGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAAc                                                                   >  4:395948/1‑150 (MQ=255)
                                                              gCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACt                                                                  <  2:652550/150‑1 (MQ=255)
                                                                acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg                                                                <  2:268662/150‑1 (MQ=255)
                                                                 caACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTgg                                                               <  4:266117/150‑1 (MQ=255)
                                                                      cgGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTc                                                          >  1:573807/1‑150 (MQ=255)
                                                                        gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcgc                                                        <  1:64686/150‑1 (MQ=255)
                                                                         tCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAg                                                                             >  3:261125/1‑128 (MQ=255)
                                                                         tCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAg                                                                             <  4:261125/128‑1 (MQ=255)
                                                                          cAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCCCAACGAGCTAACGTAACTTGGCGTTCGCGt                                                      <  2:362680/150‑1 (MQ=255)
                                                                           aaGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgc                                                                                             <  3:220139/110‑1 (MQ=255)
                                                                           aaGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgc                                                                                             >  4:220139/1‑110 (MQ=255)
                                                                             gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAtttt                                                                                                                        >  2:299679/1‑81 (MQ=255)
                                                                             gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAtttt                                                                                                                        <  1:299679/81‑1 (MQ=255)
                                                                             gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCt                                                                                                                    <  3:21952/85‑1 (MQ=255)
                                                                             gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCt                                                                                                                    >  4:21952/1‑85 (MQ=255)
                                                                             gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCt                                                   <  1:329094/150‑1 (MQ=255)
                                                                                   gCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCa                                             >  3:297406/1‑150 (MQ=255)
                                                                                        gCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCa                                        >  1:171393/1‑150 (MQ=255)
                                                                                         cTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCAt                                       >  1:11267/1‑150 (MQ=255)
                                                                                         cTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCAt                                       >  2:163062/1‑150 (MQ=255)
                                                                                         cTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCAt                                       >  2:231697/1‑150 (MQ=255)
                                                                                            cGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGt                                    <  3:395948/150‑1 (MQ=255)
                                                                                            cGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGt                                    <  1:196181/150‑1 (MQ=255)
                                                                                            cGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCATGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGt                                    <  1:658706/150‑1 (MQ=255)
                                                                                                 aCAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCa                               <  2:344564/150‑1 (MQ=255)
                                                                                                  cAAGGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAg                              >  2:306649/1‑150 (MQ=255)
                                                                                                        ccTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtga                        >  3:28933/1‑150 (MQ=255)
                                                                                                           gACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGa                                                                              <  3:11434/93‑1 (MQ=255)
                                                                                                           gACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGa                                                                              >  4:11434/1‑93 (MQ=255)
                                                                                                            aCCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCt                          <  3:205051/144‑1 (MQ=255)
                                                                                                            aCCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCt                          >  4:205051/1‑144 (MQ=255)
                                                                                                             ccAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAg                   <  2:11267/150‑1 (MQ=255)
                                                                                                             ccAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAGTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAg                   <  2:41584/150‑1 (MQ=255)
                                                                                                                  gAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcggc              <  2:171393/150‑1 (MQ=255)
                                                                                                                     gCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTg           <  4:297406/150‑1 (MQ=255)
                                                                                                                        cTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac        >  2:216123/1‑150 (MQ=255)
                                                                                                                        cTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac        >  2:170656/1‑150 (MQ=255)
                                                                                                                              gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaaa  >  4:167155/1‑150 (MQ=255)
                                                                                                                              gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaaa  <  3:167155/150‑1 (MQ=255)
                                                                                                                                 aCGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCAGCAGTGCTGATGAAggcgg               <  2:430753/134‑1 (MQ=255)
                                                                                                                                 aCGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcgg               >  1:430753/1‑134 (MQ=255)
                                                                                                                                     tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac        >  2:213064/1‑137 (MQ=255)
                                                                                                                                     tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac        <  1:213064/137‑1 (MQ=255)
                                                                                                                                       gTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGc                                                              >  2:671164/1‑81 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAA  >  NC_000913_3_KO_pgi/1166589‑1166864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: