Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 3,397,657 Δ9 bp coding (131‑139/1470 nt) rng ← ribonuclease G

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 33976568 (0.060)96 (0.750) 71/264 1.3 93.0% coding (140/1470 nt) rng ribonuclease G
?NC_000913_3_KO_pgi 3397666 = 7 (0.050)coding (130/1470 nt) rng ribonuclease G

Only 100 of 111 total aligned reads displayed.

CCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATC  >  NC_000913_3_KO_pgi/3397507‑3397802
                                                                                                                                                     |                                                                                                                                                  
ccAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGAC                                                                                                                                                    >  1:522096‑M1/1‑150 (MQ=255)
      ccGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTacac                                                                                                                                              >  2:134761/1‑150 (MQ=255)
       cGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgta                                                                                                                                             >  1:845779‑M1/1‑143 (MQ=255)
       cGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgta                                                                                                                                             <  1:1424700‑M1/150‑8 (MQ=255)
         agaTGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtaga                                                                                                                                           >  1:741905‑M1/1‑141 (MQ=255)
           aTGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagata                                                                                                                                         >  1:660093‑M1/1‑139 (MQ=255)
             gTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGGCccttgtagatatt                                                                                                                                       >  3:57029‑M1/1‑137 (MQ=255)
               cgcgCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgc                                                                                                                                     <  3:851093‑M1/150‑16 (MQ=255)
                  gcACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgccta                                                                                                                                  <  1:982176‑M1/150‑19 (MQ=255)
                      cGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATAtt                                                                                                                              >  3:587139/1‑150 (MQ=255)
                        tGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattc                                                                                                                            <  1:1350440‑M1/150‑25 (MQ=255)
                             tGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgt                                                                                                                       <  3:55760‑M1/150‑30 (MQ=255)
                             tGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgt                                                                                                                       <  3:876452‑M1/150‑30 (MQ=255)
                               cTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcg                                                                                                                     >  2:1328830‑M1/1‑119 (MQ=255)
                                ttttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgta                                                                                                                                             >  3:17281‑M1/1‑118 (MQ=255)
                                ttttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgta                                                                                                                                             <  4:17281‑M1/125‑8 (MQ=255)
                                 tttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATTTCGGATGCATGAAGAAACGCGGCTTTATCAAGCTCAATATCTACAAAAGCCGCCTGCATACCGGGAAGTGCACGACccttgtagctattgcctactattccgcctcgcg                                                                                                                   >  2:666733‑M1/1‑117 (MQ=255)
                                  ttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgc                                                                                                                  <  3:559324‑M1/150‑35 (MQ=255)
                                  ttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgc                                                                                                                  <  4:203986‑M1/150‑35 (MQ=255)
                                       cttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTc                                                                                                             >  2:655109/1‑150 (MQ=255)
                                        ttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacg                                                                                                            >  1:956951‑M1/1‑110 (MQ=255)
                                         tcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgt                                                                                                           <  1:1333125‑M1/150‑42 (MQ=255)
                                             ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaa                                                                                                       >  1:618743‑M1/1‑105 (MQ=255)
                                              cGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaat                                                                                                      >  1:921288‑M1/1‑104 (MQ=255)
                                                       ttCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttc                                                                                             >  3:311791‑M1/1‑95 (MQ=255)
                                                       ttCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttc                                                                                             <  3:850487‑M1/150‑56 (MQ=255)
                                                        tCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcc                                                                                            <  2:1440570‑M1/150‑57 (MQ=255)
                                                          ggTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgattttcctg                                                                                          <  2:1430089‑M1/150‑59 (MQ=255)
                                                          ggTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctg                                                                                          <  2:874214‑M1/150‑59 (MQ=255)
                                                          ggTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctg                                                                                          <  2:371079‑M1/150‑59 (MQ=255)
                                                           gtgtGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgc                                                                                         >  1:984627‑M1/1‑91 (MQ=255)
                                                           gtgtGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgc                                                                                         <  4:642710‑M1/150‑60 (MQ=255)
                                                            tgtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgca                                                                                        <  2:660093‑M1/150‑61 (MQ=255)
                                                              tgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgccttctattccgcgtcgcgcctcacgttcaataggaattttctgcaga                                                                                      >  1:410426‑M1/1‑88 (MQ=255)
                                                                 ggCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGTCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaata                                                                                   >  3:253342‑M1/1‑85 (MQ=255)
                                                                   cATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgccttacgttcaatatgaatttcctgcagaatacc                                                                                 <  2:741905‑M1/150‑68 (MQ=255)
                                                                          tCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaattttctgcagaataccgccatca                                                                          >  3:730904‑M1/1‑76 (MQ=255)
                                                                          tCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatca                                                                          <  1:1403982‑M1/150‑75 (MQ=255)
                                                                          tCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatca                                                                          <  2:8797‑M1/150‑75 (MQ=255)
                                                                          tCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatca                                                                          >  2:453853‑M1/1‑76 (MQ=255)
                                                                          tCGGATGCATGAAGAAACGCGGCTTTAACCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgccccacgttcaatatgaatttcctgcagaataccgccagca                                                                          >  2:480514‑M1/1‑76 (MQ=255)
                                                                             gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaata                                                                       <  3:551119‑M1/150‑78 (MQ=255)
                                                                             gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaata                                                                       >  1:44508‑M1/1‑73 (MQ=255)
                                                                             gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaata                                                                       >  1:587322‑M1/1‑73 (MQ=255)
                                                                             gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaata                                                                       >  1:735795‑M1/1‑73 (MQ=255)
                                                                             gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTGCAATATGAATTTCCTGCAGAATACCg                                                                       >  1:1390134/1‑150 (MQ=255)
                                                                                  aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgc                                                                  >  4:107352‑M1/1‑68 (MQ=255)
                                                                                  aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgc                                                                  >  4:779491‑M1/1‑68 (MQ=255)
                                                                                    gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgcca                                                                >  1:243357‑M1/1‑66 (MQ=255)
                                                                                    gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgcca                                                                >  1:872275‑M1/1‑66 (MQ=255)
                                                                                      agaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccact                                                              <  2:587322‑M1/150‑87 (MQ=255)
                                                                                      agaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccact                                                              >  4:682955‑M1/1‑64 (MQ=255)
                                                                                          aCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaatacccccatcaatatacgccactcgcg                                                          >  4:296183‑M1/1‑60 (MQ=255)
                                                                                           cgcgGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgt                                                         >  2:377194‑M1/1‑59 (MQ=255)
                                                                                            gcgGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtt                                                        >  2:798849‑M1/1‑58 (MQ=255)
                                                                                                 tttATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccga                                                   >  2:1193525‑M1/1‑53 (MQ=255)
                                                                                                 tttATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccga                                                   >  3:824956‑M1/1‑53 (MQ=255)
                                                                                                 tttATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccat                                                                            <  4:671757‑M1/125‑73 (MQ=255)
                                                                                                 tttATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccat                                                                            >  3:671757‑M1/1‑53 (MQ=255)
                                                                                                  ttATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaa                                                  <  4:79462‑M1/150‑99 (MQ=255)
                                                                                                   tATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaag                                                 >  2:515836‑M1/1‑51 (MQ=255)
                                                                                                    aTCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaagg                                                >  1:1056708‑M1/1‑50 (MQ=255)
                                                                                                    aTCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGt                                                <  1:655109/150‑1 (MQ=255)
                                                                                                     tCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggc                                               <  2:845779‑M1/150‑102 (MQ=255)
                                                                                                     tCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggc                                               >  2:319886‑M1/1‑49 (MQ=255)
                                                                                                      ccAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGttt                                              >  4:481632/1‑150 (MQ=255)
                                                                                                          cccAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttac                                          <  4:545080‑M1/150‑107 (MQ=255)
                                                                                                          cccAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgc                                                                                                                  >  2:743465‑M1/1‑44 (MQ=255)
                                                                                                          cccAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgc                                                                                                                  <  1:743465‑M1/78‑35 (MQ=255)
                                                                                                            cAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgtaggcgttacgt                                        >  1:525507‑M1/1‑42 (MQ=255)
                                                                                                            cAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgt                                        >  2:33505‑M1/1‑42 (MQ=255)
                                                                                                              atatCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgttt                                      <  1:1328830‑M1/150‑111 (MQ=255)
                                                                                                              atatCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgttt                                      >  4:601870‑M1/1‑40 (MQ=255)
                                                                                                               tatCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgcccctcgcgtttccgaaggcgttacgttta                                     >  1:63309‑M1/1‑39 (MQ=255)
                                                                                                                   tACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaat                                                                                                      <  1:380820‑M1/81‑47 (MQ=255)
                                                                                                                   tACAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaat                                                                                                      >  2:380820‑M1/1‑35 (MQ=255)
                                                                                                                    aCAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaac                                >  1:958271‑M1/1‑34 (MQ=255)
                                                                                                                     cAAAAGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaaca                               >  4:503644‑M1/1‑33 (MQ=255)
                                                                                                                      aaaaGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaa                              >  1:373718‑M1/1‑32 (MQ=255)
                                                                                                                        aaGCCGCCTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaatt                            >  2:361374‑M1/1‑30 (MQ=255)
                                                                                                                        aaGCCGCCTGCATACCCGGAAGTACACGACccttgtagat                                                                                                                                          >  2:456937‑M1/1‑30 (MQ=255)
                                                                                                                        aaGCCGCCTGCATACCCGGAAGTACACGACccttgtagat                                                                                                                                          <  1:456937‑M1/40‑11 (MQ=255)
                                                                                                                          gccgccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattca                          <  2:543755‑M1/150‑123 (MQ=255)
                                                                                                                             gccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagcc                       >  3:620482‑M1/1‑25 (MQ=255)
                                                                                                                             gccTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagcc                       >  2:1347021‑M1/1‑25 (MQ=255)
                                                                                                                               cTGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgt                     >  2:1108452‑M1/1‑23 (MQ=255)
                                                                                                                                tGCATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtc                    <  2:735795‑M1/150‑129 (MQ=255)
                                                                                                                                 gCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAAtt                   >  1:458603/1‑150 (MQ=255)
                                                                                                                                  cATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcat                  <  1:1204674‑M1/150‑131 (MQ=255)
                                                                                                                                  cATACCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcat                  <  3:367762‑M1/150‑131 (MQ=255)
                                                                                                                                     aCCCGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtt               <  3:682955‑M1/150‑134 (MQ=255)
                                                                                                                                      cccGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgttt              <  3:253090‑M1/150‑135 (MQ=255)
                                                                                                                                       gcgGAATTACACGACcgttgtagatattgactactattccgcgtcgcgcctcacgtgcaatatgaaggtcgggcagaataccgccagcaatataggccactcgcgtttccgaaggcgttacgtttactaacagttcagccgtcatgttta             >  2:497088‑M1/2‑15 (MQ=255)
                                                                                                                                       ccGGAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgttta             >  3:492799‑M1/1‑15 (MQ=255)
                                                                                                                                         ggAAGTACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcccgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatc           >  2:1232879‑M1/1‑13 (MQ=255)
                                                                                                                                           aaGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCAt         <  1:134761/150‑1 (MQ=255)
                                                                                                                                              tACACGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatcccttt      >  1:117340‑M1/1‑8 (MQ=255)
                                                                                                                                                  cGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatcccttttctc  <  1:584724‑M1/150‑147 (MQ=255)
                                                                                                                                                  cGACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatcaatatacgccactcgcgtttccgaaggcgttacgtttactaacaattcagccgtcatgtttatcccttttctc  <  3:503644‑M1/150‑147 (MQ=255)
                                                                                                                                                   gACccttgtagatattgcctactattccgcgtcgcgcctcacgttcaatatgaatttcctgcagaataccgccatca                                                                          <  3:799710‑M1/77‑75 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATC  >  NC_000913_3_KO_pgi/3397507‑3397802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.