New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 3003998110 (0.810)10 (0.090) 10/232 15.1 9.6% intergenic (+30/+10) xdhC/ygeV xanthine dehydrogenase, Fe‑S binding subunit/putative sigma‑54‑interacting transcriptional activator
?NC_000913_3_KO_pgi = 3003974 97 (0.860)intergenic (+6/+34) xdhC/ygeV xanthine dehydrogenase, Fe‑S binding subunit/putative sigma‑54‑interacting transcriptional activator
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTACCACGGCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGAT  >  NC_000913_3_KO_pgi/3003825‑3004121
                                                                                                                                                     |                                                                                                                                                   
gCTACCACGGCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGat                                                                                                                                                     <  2:1048573/150‑1 (MQ=255)
                         cACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGAtt                                                                                                                            >  3:409787/1‑150 (MQ=255)
                         cACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGAtt                                                                                                                            >  1:1071478/1‑150 (MQ=255)
                           cgcgAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCa                                                                                                                          <  1:595374/150‑1 (MQ=255)
                             cgAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACt                                                                                                                        <  2:904745/150‑1 (MQ=255)
                              gagaAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAGCGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTg                                                                                                                       <  1:129985/150‑1 (MQ=255)
                              gagaAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTg                                                                                                                       >  2:872980/1‑150 (MQ=255)
                               agaAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGa                                                                                                                      <  2:243572/150‑1 (MQ=255)
                                gaAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAg                                                                                                                     <  2:246625/150‑1 (MQ=255)
                                  aGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGTAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGt                                                                                                                   <  2:1385683/150‑1 (MQ=255)
                                     cATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTAt                                                                                                                <  4:644179/150‑1 (MQ=255)
                                      aTTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTAtg                                                                                                               >  1:204087/1‑150 (MQ=255)
                                        tAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTAtgtg                                                                                                             >  1:1425669/1‑150 (MQ=255)
                                          aCCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATCCAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGtt                                                                                                           >  1:526594/1‑150 (MQ=255)
                                          aCCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGtt                                                                                                           <  1:1372432/150‑1 (MQ=255)
                                            cATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTa                                                                                                         >  4:696222/1‑150 (MQ=255)
                                             aTTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGTATTCAGGCCGGATTCACTGAGGTTATGTGTTTaa                                                                                                        <  1:494825/150‑1 (MQ=255)
                                             aTTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTaa                                                                                                        <  1:272416/150‑1 (MQ=255)
                                             aTTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTaa                                                                                                        >  4:540269/1‑150 (MQ=255)
                                                aCGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGTTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTaacaa                                                                                                     <  3:61651/150‑1 (MQ=255)
                                                  ggAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACt                                                                                                   >  2:427503/1‑150 (MQ=255)
                                                  ggAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACt                                                                                                   >  4:21798/1‑150 (MQ=255)
                                                  ggAAATGAGTCGCGTACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACt                                                                                                   <  4:486474/150‑1 (MQ=255)
                                                           tcgCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCt                                                                                          >  2:298719/1‑150 (MQ=255)
                                                           tcgCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCt                                                                                          >  1:725097/1‑150 (MQ=255)
                                                             gcgGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTa                                                                                        <  4:346958/150‑1 (MQ=255)
                                                             gcgGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTa                                                                                        >  1:611153/1‑150 (MQ=255)
                                                                gACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATc                                                                                     <  1:427503/150‑1 (MQ=255)
                                                                gACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATGGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAAGTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATc                                                                                     >  1:718002/1‑150 (MQ=255)
                                                                  cTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCtt                                                                                   <  1:872980/150‑1 (MQ=255)
                                                                   tGGCGGGAAATCTTTGTCGATGCACGGGGTATCAGATGATTGTAAATACTGTTCTGGATTGCGAGTATACGTAGTAAAAGGATTTCCGGCCTGAATTAAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTg                                                                                  <  3:83510/150‑1 (MQ=255)
                                                                    ggCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGc                                                                                 <  3:94731/150‑1 (MQ=255)
                                                                     gCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCg                                                                                >  4:32857/1‑150 (MQ=255)
                                                                          aaaTCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGc                                                                                 >  4:265759/1‑144 (MQ=255)
                                                                          aaaTCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGc                                                                                 <  3:265759/144‑1 (MQ=255)
                                                                           aaTCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATagag                                                                          <  3:439658/150‑1 (MQ=255)
                                                                             tCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCg                                                                        >  2:112232/1‑150 (MQ=255)
                                                                             tCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCg                                                                        >  1:468879/1‑150 (MQ=255)
                                                                              cTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGACAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTAtgt                                                                                                              <  1:838986/111‑1 (MQ=255)
                                                                              cTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTAtgt                                                                                                              >  2:838986/1‑111 (MQ=255)
                                                                                ttGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAg                                                                     >  2:411490/1‑150 (MQ=255)
                                                                                ttGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATGCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAg                                                                     <  2:1129470/150‑1 (MQ=255)
                                                                                 tGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGc                                                                    <  2:725097/150‑1 (MQ=255)
                                                                                   tCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCaa                                                                  <  3:32857/150‑1 (MQ=255)
                                                                                    cGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCaac                                                                 >  1:908102/1‑149 (MQ=255)
                                                                                     gCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATg                                                                >  2:1057027/1‑150 (MQ=255)
                                                                                      cTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGc                                                               <  2:43333/150‑1 (MQ=255)
                                                                                        gCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCg                                                             >  2:1341914/1‑150 (MQ=255)
                                                                                          aCGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGAt                                                           <  1:1041986/150‑1 (MQ=255)
                                                                                          aCGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGAt                                                           >  2:381580/1‑150 (MQ=255)
                                                                                            ggggTATCAGATGATTGAAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGc                                                         <  2:1365161/150‑1 (MQ=255)
                                                                                             gggTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGcc                                                        >  2:855018/1‑150 (MQ=255)
                                                                                              ggTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGccc                                                       <  4:409787/150‑1 (MQ=255)
                                                                                                  tCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGtt                                                   <  2:301231/150‑1 (MQ=255)
                                                                                                     gatgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTg                                                                                  <  1:1031860/116‑1 (MQ=255)
                                                                                                     gatgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTg                                                                                  >  2:1031860/1‑116 (MQ=255)
                                                                                                     gatgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcat                                                >  4:238070/1‑150 (MQ=255)
                                                                                                     gatgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcat                                                >  2:78205/1‑150 (MQ=255)
                                                                                                     gatgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcat                                                >  2:1162348/1‑150 (MQ=255)
                                                                                                      atgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcatc                                               <  4:712722/150‑1 (MQ=255)
                                                                                                      atgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcatc                                               <  4:335123/150‑1 (MQ=255)
                                                                                                      atgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcatc                                               <  3:605161/150‑1 (MQ=255)
                                                                                                      atgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcatc                                               >  3:457170/1‑150 (MQ=255)
                                                                                                       tgatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTtcatca                                              <  3:540269/150‑1 (MQ=255)
                                                                                                              aaaTACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGTCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACtt                                       >  4:99636/1‑150 (MQ=255)
                                                                                                               aaTACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCTGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTg                                      >  4:347133/1‑150 (MQ=255)
                                                                                                                   cAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGcttc                                  <  1:1068383/150‑1 (MQ=255)
                                                                                                                    aGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGcttct                                 <  4:652506/150‑1 (MQ=255)
                                                                                                                    aGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGcttct                                 >  1:792986/1‑150 (MQ=255)
                                                                                                                      ttCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTg                               >  2:1239853/1‑150 (MQ=255)
                                                                                                                        ttGGAGTGCAAGAAAACGAAGTAAAAGGATACCCGGTCTGTGTTCAGGCCGTATTCATTGAGGTTATGTGCTTAACCACTCATATTTCTTATTCGTGAGAAAGGGCGTAGCAACGCCGGTGCCCATTCCATCAGCAACTTCGTCCTTGcc                             <  2:803682/149‑2 (MQ=255)
                                                                                                                          ggATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGt                           <  2:611153/150‑1 (MQ=255)
                                                                                                                           gATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGtt                          >  4:436241/1‑150 (MQ=255)
                                                                                                                            aTTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGATTACCAACTCATCTTACTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCCTGCTGTTa                         <  1:713727/150‑1 (MQ=255)
                                                                                                                             ttGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACt                                        >  1:1341416/1‑134 (MQ=255)
                                                                                                                             ttGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACt                                        <  2:1341416/134‑1 (MQ=255)
                                                                                                                               gCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGa                      <  3:243107/150‑1 (MQ=255)
                                                                                                                                ggagaAAACGAAGTAAAAGGATCTCCGGCCTCAATTCAGGCCGGAGTCACTGAGGCTATGTGTTTAACAACTCATATTTCTTATTCTTGCGATCGATCGTCGCAATGCCGATGCCCAGTTCATCAGCAACTTGGTTCTTGCTGTCAtccc                     >  2:723034/2‑147 (MQ=255)
                                                                                                                                cGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGAc                     <  4:875919/150‑1 (MQ=255)
                                                                                                                                cGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGAc                     >  2:69777/1‑150 (MQ=255)
                                                                                                                                  agaAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGt                   >  2:758560/1‑150 (MQ=255)
                                                                                                                                  agaAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGt                   >  4:587358/1‑150 (MQ=255)
                                                                                                                                    aaaaCGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGa                 >  2:822601/1‑150 (MQ=255)
                                                                                                                                     aaaCGTATTAAAAGGAGATCCGGTCTGAATCCAGGTCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCTATAGGGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGaa                <  2:526594/150‑1 (MQ=255)
                                                                                                                                     aaaCGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGaa                >  2:373970/1‑150 (MQ=255)
                                                                                                                                      aaCGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGcc                                                        <  1:1352025/109‑1 (MQ=255)
                                                                                                                                      aaCGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGcc                                                        >  2:1352025/1‑109 (MQ=255)
                                                                                                                                      aaCGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGaaa               <  2:690064/150‑1 (MQ=255)
                                                                                                                                        cGAAGTAAAAGGATATCCGGCCTTAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAGCTCATATTTCTTAATCCTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAgc             <  2:1023979/150‑1 (MQ=255)
                                                                                                                                         gAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAgcg            >  1:662606/1‑150 (MQ=255)
                                                                                                                                         gAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAgcg            >  4:726711/1‑150 (MQ=255)
                                                                                                                                          aaGTAAAAGGATATCCGGCCTGAATTCAGACCTGATTCACTGATGGTATGTGGTGAACAACTGATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAgcgc           <  1:131466/150‑1 (MQ=255)
                                                                                                                                           aGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGcc          <  2:1188642/150‑1 (MQ=255)
                                                                                                                                           aGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGcc          >  1:1333823/1‑150 (MQ=255)
                                                                                                                                               aaaGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTcgc      >  1:1333760/1‑150 (MQ=255)
                                                                                                                                                aaGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTcgcg     >  2:723703/1‑150 (MQ=255)
                                                                                                                                                  ggATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGa   >  1:621348/1‑150 (MQ=255)
                                                                                                                                                   gATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGCTCCCCAGTTCATCAGCAACTTGCTTCTTGCTGGTATGACGTGAAAGCGCCTCGCGGAt  >  4:475852/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCTACCACGGCAATGCTGGCGAAACCACGCGAGAAGCCATTAACCATTACGGAAATTCGTCGCGGACTGGCGGGAAATCTTTGTCGCTGCACGGGGTATCAGATGATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGAT  >  NC_000913_3_KO_pgi/3003825‑3004121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.