Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,840 +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA intergenic (‑607/‑500) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42338400 (0.000)163 (0.990)
+GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA
86/190 0.8 100% intergenic (‑607/‑500) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233841 = NA (NA)intergenic (‑608/‑499) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Only 100 of 193 total aligned reads displayed.

TTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATT  >  NC_000913_3_KO_pgi/4233694‑4233987
                                                                                                                                                  |                                                                                                                                                   
atcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCggaatgct                                                                                                                                                  <  1:1488615‑M2/86‑4 (MQ=255)
 tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                                                                                                 <  2:1505904‑M2/87‑5 (MQ=255)
 tcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                                                                                                 >  3:896901‑M2/64‑146 (MQ=255)
  cagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCccggaatgctcg                                                                                                                                                <  2:1689387‑M2/88‑6 (MQ=255)
    gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATccccggaatgctcgac                                                                                                                                              >  4:296406‑M2/61‑143 (MQ=255)
    gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTACTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATccccggaatgctcgac                                                                                                                                              >  3:1038028‑M2/61‑143 (MQ=255)
                                                         gcactacggtgtaggcTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatat                                                                                         >  1:1178518‑M1/8‑90 (MQ=255)
                                                           actacggtgtagGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgt                                                                                       <  4:352442‑M1/145‑63 (MQ=255)
                                                           actacggtgtagGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgt                                                                                       <  1:2409173‑M1/145‑63 (MQ=255)
                                                           actacggtgtagGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgt                                                                                       <  4:1226406‑M1/145‑63 (MQ=255)
                                                             tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag                                                                                     >  1:558235‑M1/4‑86 (MQ=255)
                                                             tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag                                                                                     >  1:2274253‑M1/4‑86 (MQ=255)
                                                             tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgac                                                                                                                                              >  1:539808‑M1/4‑86 (MQ=255)
                                                                ggTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggcc                                                                                  <  2:593743‑M1/150‑68 (MQ=255)
                                                                 gtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccg                                                                                 <  2:2254766‑M1/150‑69 (MQ=255)
                                                                  tgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccgg                                                                                >  4:1002855‑M1/1‑81 (MQ=255)
                                                                    tAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggat                                                                              >  3:189165‑M1/1‑79 (MQ=255)
                                                                      ggCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggatgc                                                                            >  2:1941576‑M1/1‑77 (MQ=255)
                                                                       gCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag                                                                           <  2:532103‑M1/150‑75 (MQ=255)
                                                                           gAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctc                                                                                                                                                 <  1:1852337‑M1/76‑5 (MQ=255)
                                                                             gctgctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttc                                                                     <  1:2058585‑M1/150‑81 (MQ=255)
                                                                              ctgctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttca                                                                    >  2:1171286‑M1/1‑69 (MQ=255)
                                                                                gctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacg                                                                  <  1:1290086‑M1/150‑84 (MQ=255)
                                                                                 ctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgc                                                                 >  2:2107364‑M1/1‑66 (MQ=255)
                                                                                 ctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcg                                                                        <  1:1383109‑M1/143‑78 (MQ=255)
                                                                                 ctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcg                                                                        >  2:1383109‑M1/1‑66 (MQ=255)
                                                                                  ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc                                                                >  2:938524‑M1/1‑65 (MQ=255)
                                                                                  ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc                                                                >  3:653508‑M1/1‑65 (MQ=255)
                                                                                  ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc                                                                >  4:1229739‑M1/1‑65 (MQ=255)
                                                                                  ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaa                                                                                                                     <  1:109816‑M1/97‑33 (MQ=255)
                                                                                    cGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgc                                                              >  2:1341379‑M1/1‑63 (MQ=255)
                                                                                     gAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgca                                                             >  3:247103‑M1/1‑62 (MQ=255)
                                                                                     gAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaag                                                                                                                    >  2:454137‑M1/1‑62 (MQ=255)
                                                                                     gAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaag                                                                                                                    <  1:454137‑M1/95‑34 (MQ=255)
                                                                                          tCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccgg                                                        >  3:1250133‑M1/1‑57 (MQ=255)
                                                                                           ccTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgct                                                                                                                          <  2:2183609‑M1/83‑28 (MQ=255)
                                                                                           ccTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgct                                                                                                                          >  1:2183609‑M1/1‑56 (MQ=255)
                                                                                             tataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaa                                                     >  2:2257866‑M1/1‑54 (MQ=255)
                                                                                              ataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaac                                                    <  1:2517733‑M1/150‑98 (MQ=255)
                                                                                              ataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaac                                                    >  4:856094‑M1/1‑53 (MQ=255)
                                                                                              ataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctga                                                                                                                                   >  4:97365‑M1/1‑53 (MQ=255)
                                                                                               tacgatctagaGAATACGAAATACGAACTGCAGGTCGACTGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgagacaaggtcaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaacc                                                   <  2:1560053‑M1/145‑99 (MQ=255)
                                                                                               taCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcaagccgcatccggcaacc                                                   >  4:1459837‑M1/1‑52 (MQ=255)
                                                                                               taCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataa                                                                            >  1:1112285‑M1/1‑52 (MQ=255)
                                                                                               taCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataa                                                                            <  2:1112285‑M1/125‑74 (MQ=255)
                                                                                               taCATTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggactgattaaccgcaataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaacc                                                   >  4:867281‑M1/1‑52 (MQ=255)
                                                                                                  tttCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgat                                                >  2:2295385‑M1/1‑49 (MQ=255)
                                                                                                  tttCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgat                                                >  2:70515‑M1/1‑49 (MQ=255)
                                                                                                    tCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCTCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc                                              <  3:1396628‑M1/150‑104 (MQ=255)
                                                                                                    tCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc                                              <  4:1038028‑M1/150‑104 (MQ=255)
                                                                                                    tCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc                                              <  1:327057‑M1/150‑104 (MQ=255)
                                                                                                    tCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc                                              >  3:1225340‑M1/1‑47 (MQ=255)
                                                                                                    tCTAAAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc                                              <  4:1630076‑M1/150‑104 (MQ=255)
                                                                                                       agagAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctg                                           >  3:379295‑M1/1‑44 (MQ=255)
                                                                                                        gagaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctga                                          <  1:1960886‑M1/150‑108 (MQ=255)
                                                                                                        gagaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctga                                          >  2:540661‑M1/1‑43 (MQ=255)
                                                                                                        gagaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctga                                          <  1:70515‑M1/150‑108 (MQ=255)
                                                                                                         agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgat                                         <  2:1367599‑M1/150‑109 (MQ=255)
                                                                                                         agaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgat                                         >  1:1698429‑M1/1‑42 (MQ=255)
                                                                                                           aaTAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgc                                       <  4:56108‑M1/150‑111 (MQ=255)
                                                                                                           aaTAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgc                                       >  2:2428987‑M1/1‑40 (MQ=255)
                                                                                                             tAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtct                                                                                                                                     >  4:745797‑M1/1‑38 (MQ=255)
                                                                                                              aGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgac                                    <  1:669924‑M1/150‑114 (MQ=255)
                                                                                                                gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctatcaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgc                                  >  3:946015‑M1/1‑35 (MQ=255)
                                                                                                                gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgc                                  >  1:2142939‑M1/1‑35 (MQ=255)
                                                                                                                 aaCTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcg                                 <  2:1780741‑M1/150‑117 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc                              <  1:301053‑M1/150‑120 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc                              <  1:770657‑M1/150‑120 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc                              >  2:796165‑M1/1‑31 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc                              >  1:394663‑M1/1‑31 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc                              >  3:809547‑M1/1‑31 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgat                                                <  2:1684511‑M1/132‑102 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaac                                                    <  4:456859‑M1/128‑98 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaac                                                    >  3:456859‑M1/1‑31 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccgaataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc                              <  4:1478027‑M1/150‑120 (MQ=255)
                                                                                                                    ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtc                                                                                                                                      <  1:828326‑M1/46‑16 (MQ=255)
                                                                                                                         cctGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctatagagcgtggcgcggttaatcatcgtcgctatgtaggccggataaggcgttcccgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtc                         <  2:411388‑M1/149‑125 (MQ=255)
                                                                                                                           tGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctt                       >  4:1419052‑M1/1‑24 (MQ=255)
                                                                                                                            gCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctta                      >  1:2264539‑M1/1‑23 (MQ=255)
                                                                                                                             cAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttat                     <  1:100188‑M1/150‑129 (MQ=255)
                                                                                                                               ggTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaggcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatca                   <  2:300646‑M1/150‑131 (MQ=255)
                                                                                                                               ggTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatca                   <  2:275356‑M1/150‑131 (MQ=255)
                                                                                                                                gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcag                  >  1:1221540‑M1/1‑19 (MQ=255)
                                                                                                                                gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcag                  >  4:331306‑M1/1‑19 (MQ=255)
                                                                                                                                gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtc                                                                                              <  2:2055554‑M1/74‑56 (MQ=255)
                                                                                                                                gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgct                                                                                                                          >  1:654045‑M1/1‑19 (MQ=255)
                                                                                                                                 tCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcagg                 >  3:860513‑M1/1‑18 (MQ=255)
                                                                                                                                   gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcct                                            <  1:2601163‑M1/121‑106 (MQ=255)
                                                                                                                                   gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcct                                            >  2:2601163‑M1/1‑16 (MQ=255)
                                                                                                                                   gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgc                                                                 <  2:1897548‑M1/100‑85 (MQ=255)
                                                                                                                                    aCGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcct              >  3:332976‑M1/1‑15 (MQ=255)
                                                                                                                                    aCGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcct              >  1:37872‑M1/1‑15 (MQ=255)
                                                                                                                                      ggATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctac            >  1:2217046‑M1/1‑13 (MQ=255)
                                                                                                                                       gATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctaca           <  3:60795‑M1/150‑139 (MQ=255)
                                                                                                                                          ccccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggt        <  1:1341379‑M1/150‑142 (MQ=255)
                                                                                                                                          atccggAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggt        <  3:1565113‑M1/148‑142 (MQ=255)
                                                                                                                                            ccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcg      >  2:1890040‑M1/1‑7 (MQ=255)
                                                                                                                                            ccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctga                                          <  3:1611605‑M1/114‑108 (MQ=255)
                                                                                                                                              ggAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcgat    >  1:631537‑M1/1‑5 (MQ=255)
                                                                                                                                                aaTgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcgatgc  <  2:824352‑M1/150‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATT  >  NC_000913_3_KO_pgi/4233694‑4233987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.