Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,840 +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA intergenic (‑607/‑500) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42338400 (0.000)163 (0.990)
+GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA
86/190 0.8 100% intergenic (‑607/‑500) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233841 = NA (NA)intergenic (‑608/‑499) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Only 100 of 266 total aligned reads displayed.

TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAA  >  NC_000913_3_KO_pgi/4233705‑4233990
                                                                                                                                        |                                                                                                                                                     
gcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGatat                                                                                                                                          >  1:1178518‑M2/137‑150 (MQ=255)
  actacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGt                                                                                                                                        <  1:2409173‑M2/16‑1 (MQ=255)
  actacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGt                                                                                                                                        <  4:352442‑M2/16‑1 (MQ=255)
  actacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGt                                                                                                                                        <  2:577243‑M2/16‑1 (MQ=255)
    tacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAg                                                                                                                                      >  1:558235‑M2/133‑150 (MQ=255)
    tacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAg                                                                                                                                      >  1:2274253‑M2/133‑150 (MQ=255)
    tacggtgtaggctggagctgcttcgaagttcctatacttactagagaataggaacttcgaactgcagggcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttgATCATCGTCGATATGTAg                                                                                                                                      >  1:763465‑M2/133‑150 (MQ=255)
        gtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCg                                                                                                                                  <  2:2254766‑M2/22‑1 (MQ=255)
         tgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCgg                                                                                                                                 <  3:152736‑M2/23‑1 (MQ=255)
         tgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCgg                                                                                                                                 >  4:1002855‑M2/128‑150 (MQ=255)
           taggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGAt                                                                                                                               >  3:189165‑M2/126‑150 (MQ=255)
                tggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggctaATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                          >  1:2599810‑M2/121‑150 (MQ=255)
                       gcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcg                                                                                                                   <  1:1290086‑M2/37‑1 (MQ=255)
                       gcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaACCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcg                                                                                                                   <  3:341334‑M2/37‑1 (MQ=255)
                         ttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                 >  1:655735‑M2/112‑150 (MQ=255)
                         ttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                 >  3:653508‑M2/112‑150 (MQ=255)
                            gaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                                                              >  3:247103‑M2/109‑150 (MQ=255)
                                    tatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  2:1419144‑M2/101‑150 (MQ=255)
                                    tatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  2:2257866‑M2/101‑150 (MQ=255)
                                    tatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  3:384682‑M2/101‑150 (MQ=255)
                                    tatactttctagagaataggaacttcgaactgcaagtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  4:147247‑M2/101‑150 (MQ=255)
                                     atactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                     >  4:856094‑M2/100‑150 (MQ=255)
                                      tactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATaa                                                                                                                             <  2:1112285‑M2/27‑1 (MQ=255)
                                      tacgatctagagaatacgaaatacgaactgcaggtcgactgatccccggaatgctcgaccaatggtctgattaaccgctataaagcgagacaaggtcaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                                    <  2:1560053‑M2/52‑1 (MQ=255)
                                      tacattctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggactgattaaccgcaataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                                    >  4:867281‑M2/99‑150 (MQ=255)
                                       actttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCg                                                                                                   >  2:288294‑M2/98‑150 (MQ=255)
                                         tttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                                 >  2:333977‑M2/96‑150 (MQ=255)
                                         tttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                                 >  2:70515‑M2/96‑150 (MQ=255)
                                           tctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                               <  1:327057‑M2/57‑1 (MQ=255)
                                           tctaaagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                               <  4:1630076‑M2/57‑1 (MQ=255)
                                               gagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                           <  1:70515‑M2/61‑1 (MQ=255)
                                                agaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                                          >  1:1698429‑M2/89‑150 (MQ=255)
                                                      ggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg                                                                                    <  1:293600‑M2/68‑1 (MQ=255)
                                                       gaacttcgaactgcaggtcgacggatccccggactgctcgaccaatggtctgcttaaccgctctaaagcgtggcgcggctaATCATCGTCGATATGTAGGCCGGATACGGCGTTCCCGCCGCATAAGGCCCCCGATGCCTGATGcgccgc                                                                                   >  2:1297675‑M2/82‑150 (MQ=255)
                                                          cttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                                >  4:266273‑M2/79‑150 (MQ=255)
                                                           ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                     >  3:456859‑M2/78‑128 (MQ=255)
                                                           ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                     <  4:456859‑M2/51‑1 (MQ=255)
                                                           ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                                 >  1:1684511‑M2/78‑132 (MQ=255)
                                                           ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                               >  2:796165‑M2/78‑150 (MQ=255)
                                                           ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                               >  3:809547‑M2/78‑150 (MQ=255)
                                                                  tgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                                        >  4:1419052‑M2/71‑150 (MQ=255)
                                                                   gcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                       >  1:2264539‑M2/70‑150 (MQ=255)
                                                                    caggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                                      <  1:100188‑M2/82‑1 (MQ=255)
                                                                     aggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                     <  4:1231537‑M2/83‑1 (MQ=255)
                                                                      ggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                                    <  1:1465928‑M2/84‑1 (MQ=255)
                                                                       gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCAtcgtc                                                                                                                                               <  2:2055554‑M2/9‑1 (MQ=255)
                                                                       gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCAtcgtc                                                                                                                                               >  1:2055554‑M2/66‑74 (MQ=255)
                                                                        tcgacggatcccctgaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAgg                                                                  <  1:2131529‑M2/86‑1 (MQ=255)
                                                                          gacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                  >  1:1897548‑M2/63‑100 (MQ=255)
                                                                          gacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                  <  2:1897548‑M2/38‑1 (MQ=255)
                                                                          gacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCt                                                                                             >  2:2601163‑M2/63‑121 (MQ=255)
                                                                             ggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                                             >  1:2217046‑M2/60‑150 (MQ=255)
                                                                              gatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa                                                            <  3:60795‑M2/92‑1 (MQ=255)
                                                                               atccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                                    >  1:502044‑M2/58‑141 (MQ=255)
                                                                                tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                          <  3:1229739‑M2/94‑1 (MQ=255)
                                                                                 atccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                         <  3:1565113‑M2/95‑1 (MQ=255)
                                                                                  cccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                        >  3:888733‑M2/55‑150 (MQ=255)
                                                                                   ccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                       >  2:1890040‑M2/54‑150 (MQ=255)
                                                                                     ggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCGTCGTCGATATGTAGGCCGGATa                                                                                                                              <  2:1350825‑M2/26‑1 (MQ=255)
                                                                                     ggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCGTCGTCGATATGTAGGCCGGATa                                                                                                                              >  1:1350825‑M2/52‑77 (MQ=255)
                                                                                       aatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCCCTCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                   <  3:1201956‑M2/101‑1 (MQ=255)
                                                                                       aatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                   <  2:824352‑M2/101‑1 (MQ=255)
                                                                                          gctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                                <  1:2433138‑M2/104‑1 (MQ=255)
                                                                                            tcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                              <  1:357755‑M2/106‑1 (MQ=255)
                                                                                            tcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                              >  1:1878602‑M2/45‑150 (MQ=255)
                                                                                             cgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                                             <  4:332976‑M2/107‑1 (MQ=255)
                                                                                              gaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                            >  1:1197003‑M2/43‑150 (MQ=255)
                                                                                               accaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                           <  4:946015‑M2/109‑1 (MQ=255)
                                                                                                ccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                           <  2:1686753‑M2/77‑1 (MQ=255)
                                                                                                  aatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                               >  4:693847‑M2/39‑95 (MQ=255)
                                                                                                     ggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                                     >  2:1230577‑M2/36‑150 (MQ=255)
                                                                                                       tctggttaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                               <  1:1256865‑M2/73‑1 (MQ=255)
                                                                                                       tctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa                                   >  2:1946883‑M2/34‑150 (MQ=255)
                                                                                                        ctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                                          >  4:1531591‑M2/33‑94 (MQ=255)
                                                                                                         tgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                             <  3:223022‑M2/43‑1 (MQ=255)
                                                                                                          gattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                                <  1:1760050‑M2/120‑1 (MQ=255)
                                                                                                           attaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                               >  1:2373691‑M2/30‑150 (MQ=255)
                                                                                                           attaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGACTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                               >  1:812930‑M2/30‑150 (MQ=255)
                                                                                                             taaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                             <  3:1136172‑M2/123‑1 (MQ=255)
                                                                                                              aaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa                            <  4:556804‑M2/124‑1 (MQ=255)
                                                                                                               accgctataaagcgtggcgcggttaATCATCGTCGATGTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                                        >  1:109410‑M2/26‑105 (MQ=255)
                                                                                                               accgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                                             <  4:1207908‑M2/91‑1 (MQ=255)
                                                                                                                 cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                         <  2:533180‑M2/127‑1 (MQ=255)
                                                                                                                 cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                                             <  2:2413417‑M2/91‑1 (MQ=255)
                                                                                                                 cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                         <  4:570780‑M2/127‑1 (MQ=255)
                                                                                                                 cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                         >  1:34771‑M2/24‑150 (MQ=255)
                                                                                                                  gctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                                 <  2:1086603‑M2/103‑1 (MQ=255)
                                                                                                                    tataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                      >  3:1304015‑M2/21‑150 (MQ=255)
                                                                                                                      taaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                    <  2:469088‑M2/132‑1 (MQ=255)
                                                                                                                       aaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTGGGCAATTAATACa                   >  2:2299855‑M2/18‑150 (MQ=255)
                                                                                                                       aaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACa                   >  2:968162‑M2/18‑150 (MQ=255)
                                                                                                                        aagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                           >  2:1759265‑M2/17‑125 (MQ=255)
                                                                                                                        aagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                  <  3:1009579‑M2/134‑1 (MQ=255)
                                                                                                                         tgcgtggcgcggttaATTATCGTTGTTATGTAGGCCGGATAAGGCGTTCACGCCGCTTCCGGTAACCGATGCCTGATGCGACGCGGTTGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa                 <  4:10347‑M2/135‑1 (MQ=255)
                                                                                                                         agcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa                 >  1:1135418‑M2/16‑150 (MQ=255)
                                                                                                                                cgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat          <  2:1372145‑M2/142‑1 (MQ=255)
                                                                                                                                 gcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt         <  1:390521‑M2/143‑1 (MQ=255)
                                                                                                                                 gcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt         <  3:147247‑M2/143‑1 (MQ=255)
                                                                                                                                    gttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgat      <  1:197107‑M2/146‑1 (MQ=255)
                                                                                                                                        atcatcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGGTGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTaaa  <  2:2127876/150‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                     
TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAA  >  NC_000913_3_KO_pgi/4233705‑4233990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.