Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,757 +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42337570 (0.000)56 (1.020)
+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC
39/186 0.4 100% intergenic (‑524/‑583) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233758 = 0 (0.000)intergenic (‑525/‑582) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCG  >  NC_000913_3_KO_pgi/4233609‑4233886
                                                                                                                                                    |                                                                                                                                 
gCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAa                                                                                                                                  >  1:1137857‑M1/1‑149 (MQ=255)
 cattcagTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAat                                                                                                                                 <  2:366292‑M1/147‑3 (MQ=255)
      agtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaa                                                                                                                            >  1:972651‑M1/1‑143 (MQ=255)
      agtagtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCACAAGAGTATTGCTAatcaaaa                                                                                                                            >  2:344265‑M1/1‑143 (MQ=255)
       gtagtTCAGCTAATAAATGCTTCACTGCGCTAGGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATATTACAAAGTCACAATTCTTAAAATAAGAAGAGTATTGTTAatgaaaaa                                                                                                                           >  2:1011551‑M1/1‑142 (MQ=255)
          gtTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                        <  1:117559‑M1/150‑12 (MQ=255)
              aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCTAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaat                                                                                                                    >  1:523719‑M1/1‑135 (MQ=255)
              aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaat                                                                                                                    <  1:503634‑M1/150‑16 (MQ=255)
               gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatc                                                                                                                   >  1:827721‑M1/1‑134 (MQ=255)
               gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacaacaatc                                                                                                                   >  1:165666‑M1/1‑134 (MQ=255)
                cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGTTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatcc                                                                                                                  >  1:234479‑M1/1‑133 (MQ=255)
                 taataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatcca                                                                                                                 >  2:854733‑M1/1‑132 (MQ=255)
                  aataaATGCTTCACTGCGCTAAGGTTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCGACAATCTTCCAAAGTTACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaa                                                                                                                <  1:664187‑M1/150‑20 (MQ=255)
                  aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaa                                                                                                                <  2:473695‑M1/150‑20 (MQ=255)
                  aaaaaatgcTTCACCTCCCTAAGGGTTAACACGCAACATTACTCTAACTGCACTAAAACCATCACATTTATCTGTGACTGGCGCTACCATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaa                                                                                                                <  1:1037238‑M1/147‑20 (MQ=255)
                   ataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCTCTATAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaac                                                                                                               >  1:302086‑M1/1‑130 (MQ=255)
                    taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacg                                                                                                              <  2:972651‑M1/150‑22 (MQ=255)
                      aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaccatcagtcacacaca                                                                                                            >  1:28176‑M1/1‑127 (MQ=255)
                      aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgca                                                                                                            >  2:255283‑M1/1‑127 (MQ=255)
                         gCTTCACGGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagac                                                                                                         <  1:840783‑M1/150‑27 (MQ=255)
                           ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccg                                                                                                       >  1:473025‑M1/1‑122 (MQ=255)
                             cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgct                                                                                                     <  2:1137857‑M1/150‑31 (MQ=255)
                                  cgcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctg                                                                                                >  2:383362‑M1/1‑115 (MQ=255)
                                   gcTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCATAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgg                                                                                               >  2:932538‑M1/1‑114 (MQ=255)
                                    cTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggc                                                                                              >  2:804249‑M1/1‑113 (MQ=255)
                                     taggggtttACACTCAACATTACGCTAACGACACTAAAACCATCACATTTTTCTGTTACTGGCGCTACAATCTTCCAACATCACAAATCTCAAAATCAGAAGAGTATTTCTAatgaaaaacatcaatccaacgcagaccgctgcctggca                                                                                             <  1:1011551‑M1/147‑39 (MQ=255)
                                     tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaacaacatcaatccaacgcagaccgctgcctggca                                                                                             <  2:384756‑M1/150‑39 (MQ=255)
                                     tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATATTTCTGTGACTGGCACTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca                                                                                             >  1:1278300‑M1/1‑112 (MQ=255)
                                     tAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAATTCAGTGTGGCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca                                                                                             <  1:42246‑M1/150‑39 (MQ=255)
                                      aaGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcag                                                                                            <  1:338974‑M1/150‑40 (MQ=255)
                                       cgggtTTACACTCAACATTACGCTATCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATGAGAAGAGTATTGCTAatgaaaaacatcaatcgaacgcagaccgctgcctggcagg                                                                                           <  1:1341036‑M1/149‑41 (MQ=255)
                                       aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcagg                                                                                           >  1:599177‑M1/1‑110 (MQ=255)
                                       aGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctgccagg                                                                                           >  1:264417‑M1/1‑110 (MQ=255)
                                          gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcac                                                                                        >  1:1022231‑M1/1‑107 (MQ=255)
                                          gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcac                                                                                        >  1:68418‑M1/1‑107 (MQ=255)
                                          gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatca                                                                                                                      <  1:694776‑M1/120‑14 (MQ=255)
                                          gTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatca                                                                                                                      >  2:694776‑M1/1‑107 (MQ=255)
                                                 cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtg                                                                                 <  1:523631‑M1/150‑51 (MQ=255)
                                                  tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggca                                                                                         >  1:705653‑M1/1‑99 (MQ=255)
                                                  tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggca                                                                                         <  2:705653‑M1/141‑43 (MQ=255)
                                                  tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca                                                                                             <  1:1415432‑M1/137‑39 (MQ=255)
                                                  tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca                                                                                             >  2:1415432‑M1/1‑99 (MQ=255)
                                                   cAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgta                                                                               <  2:103390‑M1/150‑53 (MQ=255)
                                                    aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtag                                                                              <  2:444007‑M1/150‑54 (MQ=255)
                                                     aCATTACGCTAACGGCACTAAAACCATCACATTTTGCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtagg                                                                             <  2:1247320‑M1/150‑55 (MQ=255)
                                                       aTTACGCTAACGGCACTAAAACCATCACATTTTTCTTTTTTTGTCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaactcagaccgctgcctggcaggcactacggtgttgtct                                                                           >  2:723542‑M1/1‑94 (MQ=255)
                                                       aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTTGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacacagaccgctgcctggcaggcactacgctgtaggct                                                                           >  2:730850‑M1/1‑94 (MQ=255)
                                                       aTTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggct                                                                           >  2:958261‑M1/1‑94 (MQ=255)
                                                          aCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCTAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctgga                                                                        <  2:523719‑M1/150‑60 (MQ=255)
                                                          aCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctgga                                                                        <  2:165666‑M1/150‑60 (MQ=255)
                                                          aCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctgga                                                                        <  2:473025‑M1/150‑60 (MQ=255)
                                                             cTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGTTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagct                                                                     <  2:234479‑M1/150‑63 (MQ=255)
                                                                aCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtagg                                                                             >  2:214286‑M1/1‑85 (MQ=255)
                                                                aCGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtagg                                                                             <  1:214286‑M1/139‑55 (MQ=255)
                                                                     aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaa                                                             >  2:1040906‑M1/1‑80 (MQ=255)
                                                                     aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaa                                                             <  1:913036‑M1/150‑71 (MQ=255)
                                                                     aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaa                                                             <  2:814420‑M1/150‑71 (MQ=255)
                                                                       tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagt                                                           >  2:939728‑M1/1‑78 (MQ=255)
                                                                             cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                        >  2:282004‑M1/1‑72 (MQ=255)
                                                                             cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat                                                                                                                        <  1:282004‑M1/83‑12 (MQ=255)
                                                                                cacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatact                                                  >  2:1078280‑M1/1‑69 (MQ=255)
                                                                                 acaTTTTTCTGTTACTGGCGCTAGAATTTTCCAAGGTCACAATTCTCAAAATCAGAAGAGTATTGCTGatgaaaaacatcaatccaacgcagaccgctgcctggcgggcactacggtgttggctggagctgcttcgtggttcctgtattt                                                 >  2:579070‑M1/1‑68 (MQ=255)
                                                                                 acaTTTTTCTGTGACAGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAaagaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactt                                                 >  2:1194535‑M1/1‑68 (MQ=255)
                                                                                 acaTTGGTCTGTGACAGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAacgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttccaagttcctatacgt                                                 >  2:1194557‑M1/1‑68 (MQ=255)
                                                                                  caTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatacttt                                                <  2:302086‑M1/150‑84 (MQ=255)
                                                                                     ttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttcta                                             <  2:305849‑M1/150‑87 (MQ=255)
                                                                                     ttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttcta                                             <  1:591398‑M1/150‑87 (MQ=255)
                                                                                       ttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctaga                                           <  1:1188928‑M1/150‑89 (MQ=255)
                                                                                             gACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatag                                     <  2:1336916‑M1/150‑95 (MQ=255)
                                                                                              aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagg                                    >  2:1374116‑M1/1‑55 (MQ=255)
                                                                                              aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagg                                    <  2:827721‑M1/150‑96 (MQ=255)
                                                                                              aCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttactatcattcctagagaatcgg                                    >  2:220348‑M1/1‑55 (MQ=255)
                                                                                                   cgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaactt                               <  1:248389‑M1/150‑101 (MQ=255)
                                                                                                    gcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttc                              <  2:883604‑M1/150‑102 (MQ=255)
                                                                                                      tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcga                            >  1:1098757‑M1/1‑47 (MQ=255)
                                                                                                       aCAATCTTCCAAAGTCACAATTCTCAAAATCATAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaa                           <  1:932538‑M1/150‑105 (MQ=255)
                                                                                                       aCAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctattctctagaaagtataggaacttcgaa                           <  1:1382038‑M1/150‑105 (MQ=255)
                                                                                                          aTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactg                        <  1:874914‑M1/150‑108 (MQ=255)
                                                                                                           tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgc                       <  1:854733‑M1/150‑109 (MQ=255)
                                                                                                            cTTCCAAAGTAACAATTCTCAAATTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgca                      <  2:515452‑M1/150‑110 (MQ=255)
                                                                                                             ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTCCTAatgaaaaacatcaatccaacgcagcccgctgcatgacaggcactacggactaggctggagctacttcgaaattcctatactttctagagaataggaacttcgaactgcag                     >  2:743361‑M1/1‑40 (MQ=255)
                                                                                                              tCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcagg                    >  1:674701‑M1/1‑39 (MQ=255)
                                                                                                                   aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgac               <  1:804249‑M1/150‑117 (MQ=255)
                                                                                                                   aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgac               <  2:1022231‑M1/150‑117 (MQ=255)
                                                                                                                    gTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacg              <  1:818950‑M1/150‑118 (MQ=255)
                                                                                                                       acaATTCTCCAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggat           >  2:177983‑M1/1‑30 (MQ=255)
                                                                                                                       acaATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcactccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggat           >  1:1361168‑M1/1‑30 (MQ=255)
                                                                                                                       acaATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaat                                       <  2:996977‑M1/122‑93 (MQ=255)
                                                                                                                       acaATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaat                                       >  1:996977‑M1/1‑30 (MQ=255)
                                                                                                                         aaTTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatcc         >  1:1046549‑M1/1‑28 (MQ=255)
                                                                                                                         aaTTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagacagctgcctgccaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtggacggatcc         >  1:721924‑M1/1‑28 (MQ=255)
                                                                                                                            tctcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccg      <  2:1381983‑M1/150‑126 (MQ=255)
                                                                                                                            tctcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccg      <  1:948361‑M1/150‑126 (MQ=255)
                                                                                                                              tcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccgga    >  1:997952‑M1/1‑23 (MQ=255)
                                                                                                                              tcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccgga    >  1:63887‑M1/1‑23 (MQ=255)
                                                                                                                               cAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaa   <  1:1374116‑M1/150‑129 (MQ=255)
                                                                                                                                aaaaTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactccggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaactccgaactgcaggtcgcggaatccccggaat  >  2:1280898‑M1/1‑21 (MQ=255)
                                                                                                                                        aagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctata                                                    >  2:1297456‑M1/1‑13 (MQ=255)
                                                                                                                                        aagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctata                                                    <  1:1297456‑M1/92‑80 (MQ=255)
                                                                                                                                                    |                                                                                                                                 
GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCG  >  NC_000913_3_KO_pgi/4233609‑4233886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.