| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,840 | +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA | intergenic (‑607/‑500) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233840 | 0 (0.000) | 56 (1.020) +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA |
40/186 | 0.4 | 100% | intergenic (‑607/‑500) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233841 = | NA (NA) | intergenic (‑608/‑499) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
CATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGA > NC_000913_3_KO_pgi/4233691‑4233985 | aaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactccGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTCCGAACTGCAGGTCGCGGAATCCCCGGAat > 2:1280898‑M2/68‑150 (MQ=255) actacggtgtagGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgt < 1:899152‑M1/145‑63 (MQ=255) tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag < 2:721924‑M1/147‑65 (MQ=255) tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag > 1:890193‑M1/4‑86 (MQ=255) tacggtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag > 2:142395‑M1/4‑86 (MQ=255) tacggtggAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGTAACATCGCCCTCCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtag < 1:344265‑M1/147‑65 (MQ=255) gtgtAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtttgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccg < 1:958261‑M1/150‑69 (MQ=255) ggCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataa > 2:1288854‑M1/1‑77 (MQ=255) gCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatttaggccggctaag > 1:140008‑M1/1‑76 (MQ=255) cTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataagg > 2:769321‑M1/1‑75 (MQ=255) tGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcctcgtcgatatgtcggccggataaggc < 1:1194557‑M1/150‑77 (MQ=255) tGGAGCTGCTTCGAAGTTCCTATACTTGCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgttataaagcgtggcgcggttaatctccgtcgatatgttggccggataaggc < 1:1194535‑M1/150‑77 (MQ=255) ctTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGGCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgc < 1:1133789‑M1/150‑85 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc > 1:738977‑M1/1‑65 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc < 1:1338008‑M1/150‑86 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc > 2:357768‑M1/1‑65 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgcc > 2:508091‑M1/1‑65 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcga > 1:1319792‑M1/1‑65 (MQ=255) ttCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcga < 2:1319792‑M1/71‑7 (MQ=255) gAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgca > 2:932324‑M1/1‑62 (MQ=255) aaGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcat > 1:990440‑M1/1‑61 (MQ=255) aaGTTCATATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcat > 2:187586‑M1/1‑61 (MQ=255) gTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgtacacgccgcatcc > 1:1208382‑M1/1‑59 (MQ=255) attcCTAAACTTACTAGAAAAAAGGAACTTCGAACTACAAGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatcc < 1:579070‑M1/149‑92 (MQ=255) cTATACTTTCTAGAGAATAGGAACTTCGAACTGCAAGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggca > 2:1224187‑M1/1‑55 (MQ=255) tataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCGCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcg > 1:134979‑M1/1‑54 (MQ=255) tataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaa > 2:287158‑M1/1‑54 (MQ=255) tataCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcg < 2:134979‑M1/131‑78 (MQ=255) cTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccga < 1:769321‑M1/150‑101 (MQ=255) tCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgc < 1:177983‑M1/150‑104 (MQ=255) gagaATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctga > 1:1093061‑M1/1‑43 (MQ=255) tAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcga < 2:448839‑M1/150‑113 (MQ=255) gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgc > 1:315609‑M1/1‑35 (MQ=255) gAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgc > 2:1387369‑M1/1‑35 (MQ=255) ttCGAACTGCAGGTCGACGGTGCCCCTGAATtctcgatcaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc < 2:1133998‑M1/150‑120 (MQ=255) ttCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtc > 2:239553‑M1/1‑31 (MQ=255) tCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcg < 1:449665‑M1/150‑121 (MQ=255) gAACTGCAGGTCGACGGATCCCCGGAATgatagaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcg > 2:1004160‑M1/1‑28 (MQ=255) aaCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgt < 1:672688‑M1/150‑124 (MQ=255) aaCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgt < 2:738977‑M1/150‑124 (MQ=255) aaCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtc > 2:112994‑M1/1‑27 (MQ=255) aaCTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtc < 1:112994‑M1/82‑56 (MQ=255) cTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtct < 1:932324‑M1/150‑126 (MQ=255) tGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggctcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctt > 1:1145574‑M1/1‑24 (MQ=255) tGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctt < 1:730850‑M1/150‑127 (MQ=255) tGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtctt < 1:723542‑M1/150‑127 (MQ=255) ggTTGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgataagacgcgaccgcgtcgcatca < 1:383362‑M1/150‑131 (MQ=255) gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcag < 1:70206‑M1/150‑132 (MQ=255) gcgACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcagg < 2:228811‑M1/149‑133 (MQ=255) gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgccgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcc > 2:481105‑M1/1‑16 (MQ=255) gggtcccCGGAATgctcgacccatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatgtgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctac < 1:1040906‑M1/147‑138 (MQ=255) gATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgataagacgcgaccgcgtcgcatcaggcatcgg < 1:142395‑M1/150‑139 (MQ=255) ccccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggt < 1:1252358‑M1/150‑142 (MQ=255) cccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc < 1:1224187‑M1/150‑143 (MQ=255) cccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc < 2:1159134‑M1/150‑143 (MQ=255) ccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggatacggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggc < 2:903421‑M1/140‑134 (MQ=255) ccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcg > 1:1405752‑M1/1‑7 (MQ=255) ccGGAATgctcaaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggc > 1:903421‑M1/1‑7 (MQ=255) ggAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcatccagggctgt < 2:15895‑M1/150‑146 (MQ=255) | CATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGA > NC_000913_3_KO_pgi/4233691‑4233985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |