New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	951207 =	45 (1.100)	6 (0.150)	6/270	4.5	13.2%	intergenic (‑127/+65)	pflA/pflB	pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
	?	NC_000913_3_KO_pgi	951233 =	36 (0.920)					intergenic (‑153/+39)	pflA/pflB	pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAA  >  NC_000913_3_KO_pgi/951061‑951337                                                                                                                                                   |                                                                                                                                   aTGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTCGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCCCCTTACAGTATCTATAGCTGACAAAAAAGGCTCTCCCGCTaaaaca                                                                                                                                  >  2:30920/1‑147 (MQ=255)    cGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCCGACAAAAAAGGCTCTCGCGCTAAAAAAGGc                                                                                                                               <  2:37593/149‑1 (MQ=255)        cAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAAGCTCTCGCGCTAAAAAAGAccccc                                                                                                                           >  2:210726/1‑148 (MQ=255)            aaCTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACttt                                                                                                                       <  1:310319/149‑1 (MQ=255)             aCTGACATTGCGGTGTTTCCCCAGATGTGGCCCATCGGTGGCAGTGTGTTCGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTc                                                                                                                      <  1:349480/149‑1 (MQ=255)               tGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGt                                                                                                                    <  1:524923/149‑1 (MQ=255)                   aTTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTTTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAACAAAGGCCCAACTTTCGTGGAg                                                                                                                >  2:143595/1‑149 (MQ=255)                        ggTGTTTCTCCAGATGTGGCCCAGCTGAGGCCTTGTTGGTGCGCATCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTATCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCttc                                                                                                           >  1:573539/1‑148 (MQ=255)                         gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCAc                                                                                                                          >  2:79500/1‑133 (MQ=255)                         gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCAc                                                                                                                          <  1:78830/133‑1 (MQ=255)                         gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCAc                                                                                                                          <  1:79500/133‑1 (MQ=255)                         gtgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCAc                                                                                                                          >  2:78830/1‑133 (MQ=255)                                             cATCTGAGGCCGTGTTGGTGCGCAGCGCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTAc                                                                                      <  1:6471/149‑1 (MQ=255)                                              aTCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                                 >  1:46282/1‑121 (MQ=255)                                              aTCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                                 <  2:46282/121‑1 (MQ=255)                                                   aGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTc                                                                                >  1:34673/1‑149 (MQ=255)                                                    ggCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGCtttaa                                                                               >  2:679249/1‑147 (MQ=255)                                                    ggCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCa                                                                               >  2:137125/1‑149 (MQ=255)                                                       cGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTc                                                                            <  2:316093/149‑1 (MQ=255)                                                        gtgtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCa                                                                           >  2:596115/1‑149 (MQ=255)                                                               tGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTaa                                                                    <  1:628408/149‑1 (MQ=255)                                                                  gcAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTa                                                                 <  1:210726/149‑1 (MQ=255)                                                                             gCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAg                                                      <  1:644271/149‑1 (MQ=255)                                                                               tACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                                 <  1:695291/88‑1 (MQ=255)                                                                               tACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGa                                                                                                                 >  2:695291/1‑88 (MQ=255)                                                                                  gTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAg                                                 <  1:233553/149‑1 (MQ=255)                                                                                  gTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGAAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAg                                                 <  1:679249/149‑1 (MQ=255)                                                                                        tCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGa                                           <  1:137125/149‑1 (MQ=255)                                                                                            ttttGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGACGGTACGAGtaa                                       >  2:23972/1‑149 (MQ=255)                                                                                                 gCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACg                                  >  1:407349/1‑149 (MQ=255)                                                                                                   aGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTc                                >  2:551990/1‑149 (MQ=255)                                                                                                     tCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACACACATTGAGTGAAGGTACGAGTAATAACGTCct                              >  1:459598/1‑149 (MQ=255)                                                                                                     acACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATACCGTCct                              >  2:671761/2‑149 (MQ=255)                                                                                                       aCCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCaaa                                                                         <  2:18028/104‑1 (MQ=255)                                                                                                       aCCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCaaa                                                                         >  1:18028/1‑104 (MQ=255)                                                                                                                tataGATAGCTGACAAAAAAGGCCCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGATTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTAAAGGTACGAGTAATAACGTCATGCTGCTGTTCt                   >  2:343584/1‑149 (MQ=255)                                                                                                                 atagcaagCGGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCCTGGAGCCTTTATTTTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTCCTGTTCtt                  <  2:489574/143‑1 (MQ=255)                                                                                                                     atagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTCATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGt              <  2:531001/149‑1 (MQ=255)                                                                                                                           gACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTTAGTGAAGGTACGAGTAATAACGTCCTACTACTGTTCTTTAATAAGCGa        >  1:300119/1‑149 (MQ=255)                                                                                                                            aCAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAg       >  1:543800/1‑149 (MQ=255)                                                                                                                              aaaaaaGGTTGTGGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTAGATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt     <  2:302170/149‑1 (MQ=255)                                                                                                                              aaaaaaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt     >  2:633861/1‑149 (MQ=255)                                                                                                                                 aaaGGCTCTCGCGCTAAAAAAGGCCCCACTTTAGTGACGTGTACATGGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCAAGTTGaa  >  2:665352/1‑149 (MQ=255)                                                                                                                                                 aaaaaGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAtt                                                                                  <  1:442015/53‑1 (MQ=255)                                                                                                                                                 aaaaaGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGAtt                                                                                  >  2:442015/1‑53 (MQ=255)                                                                                                                                                   |                                                                                                                                   ATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAA  >  NC_000913_3_KO_pgi/951061‑951337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.