New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	1096961 =	59 (1.440)	3 (0.110) +GGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTT	3/188	4.4	8.1%	intergenic (+132/+604)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
	?	NC_000913_3_KO_pgi	1097062 =	44 (1.080)					intergenic (+233/+503)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CATTTTAATAAGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGC  >  NC_000913_3_KO_pgi/1096815‑1097107                                                                                                                                                   |                                                                                                                                                   cATTTTAATAAGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCAccc                                                                                                                                                  >  2:650544/1‑149 (MQ=255)  aTTTTAATAAGTGAGGAAGTGATAGAAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCg                                                                                                                                                 >  2:123617/1‑149 (MQ=255)    tttAATAAGTGAGGACGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGCCAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgt                                                                                                                                               >  2:86908/1‑149 (MQ=255)       aataaGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgtgtg                                                                                                                                            <  2:157238/149‑1 (MQ=255)       aataaGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACGTAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgtgtg                                                                                                                                            <  2:541192/149‑1 (MQ=255)         taaGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGCTTTCTCATCTCCCTAACAGACGTGGGCGGGGGCACCCGGtgtgtg                                                                                                                                          <  1:713806/149‑1 (MQ=255)             tGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgtgtgtaaaat                                                                                                                                      >  1:702767/1‑144 (MQ=255)                ggAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCAGTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAAtata                                                                                                                                   >  1:66236/1‑149 (MQ=255)                    gTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGTAATCTACGaa                                                                                                                               >  1:330014/1‑149 (MQ=255)                     tGATAGGAAGTGACCCGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGaaa                                                                                                                              <  1:136141/149‑1 (MQ=255)                           gAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAg                                                                                                                        >  2:388941/1‑149 (MQ=255)                                  ccAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTAc                                                                                                                 >  2:113457/1‑149 (MQ=255)                                                     tCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAACAGCCCGTACTTTCGTCCGAAGTcttctt                                                                                              >  1:338414/1‑149 (MQ=255)                                                     tCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTcttctt                                                                                              <  1:16486/149‑1 (MQ=255)                                                       tGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTa                                                                                            <  1:292274/149‑1 (MQ=255)                                                                         tgggttgctgACCGAGTAACCGGAAAGTTGAGTTTCACGATTTCCCCTCGCCCTAACAGACGTGGGCTGGGGCACGCGGTGTGGGCAATATACTAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAggggg                                                                          <  2:507812/145‑1 (MQ=18)                                                                          tGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                         >  2:452463/1‑149 (MQ=255)                                                                           gATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAggggggg                                                                        <  1:481652/149‑1 (MQ=255)                                                                              tgttgACGTAGTAACCAGACAGTTGATGTGGCGCATTTCCCCGCGCTCAAACAGACAGGGGCGGGGGCCGCCGGTGTGGGCAATATACGAAAAAAAAGCCCGTACTTTGGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAtt                                                                     <  2:659780/149‑1 (MQ=17)                                                                                ttgACTGAGTAACCAGGCAGTAGAGGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   <  1:479395/149‑1 (MQ=255)                                                                                ttgACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   <  1:696673/149‑1 (MQ=255)                                                                                  gACTGAGTAAGCAGACAGGTGATGGGCACGATTGCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGTAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACt                                                                 <  2:330014/149‑1 (MQ=21)                                                                                  gACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACt                                                                 <  1:406508/149‑1 (MQ=255)                                                                                     tGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgc                                                              >  1:316039/1‑149 (MQ=255)                                                                                        ggaaCCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttc                                                           <  1:307538/147‑1 (MQ=255)                                                                                            ccAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctc                                                       >  1:110748/1‑149 (MQ=255)                                                                                             cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCtctt                                                                                                 <  2:71368/106‑1 (MQ=255)                                                                                             cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCtctt                                                                                                 >  1:71368/1‑106 (MQ=255)                                                                                             cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTACATATGGCGGTGAGGGGGGGTTTGACTCGCTtcgctcg                                                      >  1:414888/1‑149 (MQ=255)                                                                                             cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctcg                                                      >  1:428624/1‑149 (MQ=255)                                                                                                  aGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggg                                                                           >  2:508385/1‑123 (MQ=255)                                                                                                  aGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggg                                                                           <  1:508385/123‑1 (MQ=255)                                                                                                    ttGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCg                                               >  1:192175/1‑149 (MQ=255)                                                                                                       aTGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAAtata                                                                                                                                   <  1:281559/62‑1 (MQ=255)                                                                                                       aTGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAAtata                                                                                                                                   >  2:281559/1‑62 (MQ=255)                                                                                                        tgttCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTGTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCa                                           <  1:475276/149‑1 (MQ=255)                                                                                                          tgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGc                                         >  1:521292/1‑149 (MQ=255)                                                                                                            cACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGccc                                       >  2:104912/1‑149 (MQ=255)                                                                                                            cACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGccc                                       >  1:577063/1‑149 (MQ=255)                                                                                                                    ccccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTg                               >  2:432545/1‑149 (MQ=255)                                                                                                                     cccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTc                                                                                                             >  2:285870/1‑70 (MQ=255)                                                                                                                     cccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTc                                                                                                             <  1:285870/70‑1 (MQ=255)                                                                                                                       cTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGGACGTACATGCGTTCGTGAGCCATAGGAAATATGGCGGTGCTGGGGAGATTGACCCTCGTCACTCGCCCGGCGGGCAGCGCGCTCACTTCGt                            >  1:534506/1‑149 (MQ=12)                                                                                                                       cTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTCCCTTCTTTCGAGATAGTACGTAAATATGGCGGTGACGGGGGGATTGACTCGCTTCTCGCGCCCCGCGGGCAGCCCGCTCACTGCGt                            >  1:534103/1‑149 (MQ=17)                                                                                                                       cTCCCCCTAACAGCCGTGGGCGGGGGCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGt                            <  1:187083/149‑1 (MQ=255)                                                                                                                         cGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTc                          <  2:521292/149‑1 (MQ=255)                                                                                                                           cccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAc                        <  1:113457/149‑1 (MQ=255)                                                                                                                           cccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAc                        <  2:110748/149‑1 (MQ=255)                                                                                                                            ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg                       >  2:422145/1‑149 (MQ=255)                                                                                                                               aaCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGCCAGCCCGCTAACTGCGTTCACGGTc                    >  1:710241/1‑149 (MQ=255)                                                                                                                                   gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGCGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc                >  1:685648/1‑149 (MQ=255)                                                                                                                                      gTGCGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCaa             <  1:86908/149‑1 (MQ=38)                                                                                                                                            ggggtCACCCGGTGTGGGCAATATACGAAAAAAAAGCCCGTACTTTCGTACCAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCt       <  1:611560/149‑1 (MQ=12)                                                                                                                                            gggggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   <  2:312051/89‑1 (MQ=39)                                                                                                                                            gggggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   >  1:312051/1‑89 (MQ=39)                                                                                                                                             ggggCACCCGGTGTGTACAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTg      <  2:702767/149‑1 (MQ=21)                                                                                                                                               ggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTc    >  2:34069/1‑149 (MQ=32)                                                                                                                                               ggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTc    >  1:128478/1‑149 (MQ=32)                                                                                                                                                 cACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGc  <  2:128478/149‑1 (MQ=31)                                                                                                                                                   |                                                                                                                                                   CATTTTAATAAGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGC  >  NC_000913_3_KO_pgi/1096815‑1097107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.