| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_KO_pgi | 668,742 | C→G | E99Q (GAA→CAA) | rsfS ← | ribosomal silencing factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_KO_pgi | 668,742 | 0 | C | G | 97.4% | 117.3 / ‑5.6 | 38 | E99Q (GAA→CAA) | rsfS | ribosomal silencing factor |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (19/18); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCA > NC_000913_3_KO_pgi/668597‑668881 | gccggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATa > 1:216964/1‑149 (MQ=255) ggAATTTCAATCAGCTTGAAGGGCATATCTTTCGGAAAAGGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAg < 1:291055/149‑1 (MQ=255) tCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctc > 2:395345/1‑149 (MQ=255) cAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCCTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCAAGTTGATCCAGGCGACGGctct > 2:497174/1‑149 (MQ=255) gcGAAGGCCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc < 2:502966/148‑1 (MQ=255) gAAGGGCCTAGCTTTCGGAAAACGAGGTAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCt < 2:133215/149‑1 (MQ=255) ggCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt < 2:405168/149‑1 (MQ=255) cATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGa < 2:537910/149‑1 (MQ=255) atCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTATCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGGGCCGGCTCTCTTCCAGCATGACAt > 1:27938/1‑149 (MQ=255) tCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATg < 2:387746/149‑1 (MQ=255) tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa > 2:469328/1‑149 (MQ=255) aaaaCGACGCAGGGACTCGGTAAAACCGGTTTTTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGTTACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATca < 1:432986/149‑1 (MQ=255) aaCGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGCCGGCTCTCTTCCTGCATGACATGGACAATcaca > 1:77152/1‑149 (MQ=255) cgtcggaGGTCCTCGGTAAATCCGGTTTGTACTCAGTTCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACGCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTTTTCCTGCTTTACATGGACAATCCCATc < 2:158685/143‑1 (MQ=255) gacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCg > 1:110511/1‑149 (MQ=255) aGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaa > 2:710235/1‑149 (MQ=255) ggTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaaa < 2:414322/149‑1 (MQ=255) ccGGCAAAACCTGTTTGTACCCAGTCCGGCATTTTCGTTCCCACTGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCTAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc < 1:454442/148‑1 (MQ=255) aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt > 1:439309/1‑149 (MQ=255) cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAg > 1:369416/1‑149 (MQ=255) cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTg > 2:595649/1‑149 (MQ=255) ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGGCGGCGCTGt > 1:630186/1‑149 (MQ=255) ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCt > 1:6558/1‑149 (MQ=255) gTTCCCACGGCGACAAGTTGCAGCGTCACGCATTAACTCCAGAGTGTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc < 2:676794/149‑1 (MQ=255) ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGc > 1:593909/1‑149 (MQ=255) aaGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAAt > 1:138299/1‑95 (MQ=255) aaGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAAt < 2:138299/95‑1 (MQ=255) gTTGCAGCTTCACGCATTACCTCCAGAGTTCTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGccc < 1:497174/149‑1 (MQ=255) gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCACACCTCCAGACAATGGTGGACGATCACACCGACCACAACAACGGCGATGTATGCTGCTGTGTGTTTTCCTTCTACGCCGAGCGGTGACAGGCCCGCt > 2:516375/1‑149 (MQ=255) ctgcACGCAGTAACTACAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgcgc < 2:274538/146‑1 (MQ=255) cTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgcgc > 2:285606/1‑149 (MQ=255) cTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgcgc < 2:77152/149‑1 (MQ=255) cATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTc < 1:469328/149‑1 (MQ=255) aTTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc < 2:195698/73‑1 (MQ=255) aTTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc > 1:195698/1‑73 (MQ=255) cAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCTGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAAc < 1:710235/149‑1 (MQ=255) agagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACg > 2:540303/1‑149 (MQ=255) tttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACCTCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCa < 1:19096/149‑1 (MQ=255) | GCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCA > NC_000913_3_KO_pgi/668597‑668881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |