Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 948,983 A→T Y196N (TAT→AAT)  ycaN ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi948,9830AT97.6% 127.1 / NA 43Y196N (TAT→AAT) ycaNLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base T (25/16);  minor base C (0/1);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGACGATGCAA  >  NC_000913_3_KO_pgi/948838‑949128
                                                                                                                                                  |                                                                                                                                                 
aTCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAGATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACg                                                                                                                                                >  1:94243/1‑149 (MQ=255)
aTCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACg                                                                                                                                                >  1:525588/1‑149 (MQ=255)
    gTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGaaaa                                                                                                                                            >  1:677187/1‑149 (MQ=255)
           gagCAGATACCCGATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAacac                                                                                                                                     >  2:366805/1‑149 (MQ=255)
                 aTACCCGATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATtgat                                                                                                                               <  1:606680/149‑1 (MQ=255)
                       gATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGACGCTGGGACTACGAAAAACAACACATTGATGATTag                                                                                                                         >  2:176967/1‑148 (MQ=255)
                        aTACCAGCACCCATCAATACGTTCCTCC‑GTTCAGCATCAACATCAGTCAGAATGCTCTTTCCTGCGACAGTAGTTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGTTGATTAAg                                                                                                                        <  2:611552/149‑1 (MQ=255)
                        aTACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAg                                                                                                                        <  2:252482/149‑1 (MQ=255)
                        aTACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACAAAAAACAACACATTGATGATTAAg                                                                                                                        >  2:274267/1‑149 (MQ=255)
                            cAGCACCCATCAAAACG‑GCATCCAGTTCAGCATCAAGATCATCCAGAATGATATTTCCTGCGCCAGCAATTTTCAGCTCCTTTGCAAATTGCCAGTGGAATGGTTGGCCGCTGGGCTTACGAAAATCACCACAATGATGATTAAGCAGa                                                                                                                    >  2:715680/1‑149 (MQ=255)
                                aCCCATCAAAACG‑GCCTGCAGTTCAGCATCAACATCATCCAGAATGATATTGCCTGAGACAGAAATTTCAATCTGCTTTGCAAATTGCCAGTGGAAATGTTTTCTGTTAAGATAACGAAAAACAACACATTGATGATTAAGCAGATCAt                                                                                                                <  2:535403/149‑1 (MQ=255)
                                   cATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAATGTTTGCTGCAGGGATTACGAAAAACAATACATTGATGATTCAGCAGATCATGAg                                                                                                             >  1:621802/1‑149 (MQ=255)
                                   cATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAg                                                                                                             <  2:586149/149‑1 (MQ=255)
                                         aaCG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATtg                                                                                                                                 >  2:657109/1‑123 (MQ=255)
                                         aaCG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATtg                                                                                                                                 <  1:657109/123‑1 (MQ=255)
                                          aCG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATCTTTCCTGCGACAGCCATTTGCATCTCCTTTGCAATTTGCCAGTTGAAAGGTTTGCCACTGGAATTAAAAAAAACAACACATTGATGATTAACCCGATCATGAGGGTGGca                                                                                                      >  2:82877/1‑148 (MQ=255)
                                          aCG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACATCCACTTCCATCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCg                                                                                                      >  1:141362/1‑149 (MQ=255)
                                          aCG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGGTGGCg                                                                                                      >  1:572894/1‑149 (MQ=255)
                                              gCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCgggg                                                                                                   <  2:677187/149‑1 (MQ=255)
                                                 tCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGttt                                                                                                >  2:264674/1‑149 (MQ=255)
                                                   cAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGc                                                                                              <  1:187635/149‑1 (MQ=255)
                                                     gTTCAGCATGAACATCATCCAGAAGCATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCa                                                                                            <  2:215712/149‑1 (MQ=255)
                                                            aTCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTg                                                                                     >  1:291347/1‑149 (MQ=255)
                                                                       ccAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTActct                                                                          >  2:465556/1‑149 (MQ=255)
                                                                        cAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCAGGGTTTGCCATATCTTGCAAAGTACTCTg                                                                         <  2:196380/149‑1 (MQ=255)
                                                                          gAATGCTATTTCCTGCGACAGCAATTTCCAGCTCCTGTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACCCAACAATAATCATTACGCCGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGc                                                                       <  2:660999/149‑1 (MQ=255)
                                                                             tGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTCCTCTGGCGtt                                                                    >  2:477230/1‑149 (MQ=255)
                                                                             tGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGATTTGCCATATCTTGCAAAGTACTCTGGCGtt                                                                    >  1:384001/1‑149 (MQ=255)
                                                                                  tttCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTtgctgc                                                               >  1:188485/1‑149 (MQ=255)
                                                                                             aGCAATTTCCAGCTGCGTTGCAAATTGCCCGTGGAAAGGTTTGCCACTGAGATTACGAAAAATAACACATTGATGATTAAGCAGAGCATGAGGATGGCGGAGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTCCACATAAtt                                                    >  2:653115/1‑149 (MQ=255)
                                                                                                    tCCAGCTCCTTTTCAAACTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTgg                                             <  1:530029/149‑1 (MQ=255)
                                                                                                       aGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCgg                                          >  1:522100/1‑149 (MQ=255)
                                                                                                                   aaTTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATg                                                                                                               <  2:361348/68‑1 (MQ=255)
                                                                                                                   aaTTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATg                                                                                                               >  1:361348/1‑68 (MQ=255)
                                                                                                                   aaTTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCAc                              <  1:148266/149‑1 (MQ=255)
                                                                                                                           gTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCgggg                                                                                                   >  2:519336/1‑72 (MQ=255)
                                                                                                                           gTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCgggg                                                                                                   <  1:519336/72‑1 (MQ=255)
                                                                                                                                 aGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCtt                >  1:511275/1‑149 (MQ=255)
                                                                                                                                    tttGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCttttt             >  1:563671/1‑149 (MQ=255)
                                                                                                                                     tttccGCTGGGATCACGAAAAACAACACATTGATGATTAATCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTc            <  2:141362/146‑1 (MQ=255)
                                                                                                                                          gcggggATTACGAAACACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTGCTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTcgacga       <  2:403540/146‑1 (MQ=255)
                                                                                                                                           cTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGACGAt      >  1:362495/1‑149 (MQ=255)
                                                                                                                                               gATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGACGATGCaa  <  2:511275/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACG‑GCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGACGATGCAA  >  NC_000913_3_KO_pgi/948838‑949128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑